作者： Zhu, Shirong;Wang, Xiaoling;Liu, Gaoqiang

期刊： Toxics,2024年12(6):378- ISSN：2305-6304

通讯作者： Wang, XL

作者机构： [Liu, Gaoqiang; Wang, XL; Wang, Xiaoling; Zhu, Shirong] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;[Liu, Gaoqiang; Wang, XL; Wang, Xiaoling; Zhu, Shirong] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.

通讯机构： [Wang, XL ] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Peoples R China.

关键词： Ganoderma lucidum peptide;cadmium poisoning;lung injury;NLRP3 inflammasome

摘要： Ganoderma triterpenes and spore powder have shown promising results in mitigating cadmium-induced renal and hepatic injuries. Ganoderma lucidum active peptide GLP4 is a natural protein with dual antioxidant activities derived from the mycelium of Ganoderma lucidum. However, its efficacy in alleviating cadmium-induced lung injury remains unexplored. This study aims to investigate the protective effects of GLP4 against cadmium-induced lung injury in mice. Mice were exposed to cadmium chloride via nebulization to induce lung injury. The protective effect of GLP4 was assessed by measuring the total cell count in BALF, levels of inflammatory cytokines, and the expression of NLRP3 in lung tissues a through histopathological examination of lung tissue changes. The results showed that GLP4 significantly mitigated histopathological damage in lung tissues, decreased the secretion of inflammatory cytokines, and reduced the expression of NLRP3, which was elevated in cadmium-exposed mice. In vitro studies further revealed that GLP4 inhibited the cadmium-induced activation of the NLRP3 inflammasome. Notably, acute cadmium exposure by the respiratory tract did not affect the liver and kidneys of the mice. The findings suggest that GLP4 reduces cadmium-induced lung injury in mice by inhibiting the activation of the NLRP3 inflammasome, which provides a theoretical foundation for using Ganoderma lucidum as a preventive and therapeutic agent against cadmium poisoning.

语种： 英文

作者： Shi, Muling;Mi, Xujun;Huang, Ling;Qiu, Liping;Yang, Liu;...

期刊： Analytical Chemistry,2023年95(21):8357-8366 ISSN：0003-2700

通讯作者： Liu, GQ

作者机构： [Liu, GQ; Yang, Yingzhuo; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Liu, GQ; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang; Yang, Liu] Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.;[Sun, Xing; Shi, Muling; Qiu, Liping; Yang, Liu] Hunan Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Chem & Mol Med, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.;[Shi, Muling; Hong, Chao] Hainan Univ, Coll Mat Sci & Engn, Haikou 570228, Hainan, Peoples R China.

通讯机构： [Liu, GQ ] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.

摘要： Ganoderma is a prize medicinal macrofungus with a broad range of pharmaceutical values. To date, various attempts have been made to cultivate Ganoderma to improve the production of secondary metabolites with pharmacological activity. Among the adopted techniques, protoplast preparation and regeneration are indispensable. However, the evaluation of protoplasts and regenerated cell walls usually relies on electron microscopy assays, which require time-consuming and destructive sample preparation and merely provide localized information in the selected area. In contrast, fluorescence assays enable sensitive real-time detection and imaging in vivo. They can also be applied to flow cytometry, providing a collective overview of every cell in a sample. However, for macrofungi such as Ganoderma, the fluorescence analysis of protoplasts and regenerated cell walls is difficult owing to the hindrance of the homologous fluorescent protein expression and the lack of an appropriate fluorescence marker. Herein, a specific plasma membrane probe, TAMRA perfluorocarbon nucleic acid probe (TPFN), is proposed for the nondestructive and quantitative fluorescence analysis of cell wall regeneration. Exploiting the perfluorocarbon membrane-anchoring chains, hydrophilic nucleic acid linker, and fluorescent dye TAMRA, the probe is proven to be selective, soluble, and stable, enabling rapid fluorescence detection of a protoplast sample free of transgenic expression or immune staining. Based on the TPFN and flow cytometry techniques, a quantitative approach is constructed to monitor the process of cell wall growth in a fast, quantitative, and high-throughout manner, and the obtained results are consistent with those of conventional electron microscopy. In principle, with slight modifications or integration, the proposed probe and approach can be adapted to the preparation of cell protoplasts, inspection of cell wall integrity under environmental stress, and programmable membrane engineering for cytobiology and physiology research. © 2023 American Chemical Society. All rights reserved.

语种： 英文

作者： Ma, You-Chu;Huang, Ping;Wang, Xiao-Ling;Liu, Gao-Qiang

期刊： Bioresource Technology,2023年373:128705 ISSN：0960-8524

通讯作者： Liu, Gao-Qiang(gaoliuedu@csuft.edu.cn)

作者机构： [Huang, Ping; Liu, Gao-Qiang; Ma, You-Chu; Wang, Xiao-Ling] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.;[Huang, Ping; Liu, Gao-Qiang; Ma, You-Chu; Wang, Xiao-Ling] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Liu, Gao-Qiang; Ma, You-Chu; Wang, Xiao-Ling] Microbial Variety Creat Ctr, Hunan Prov Lab Yuelushan Seed Ind, Changsha 410004, Peoples R China.

通讯机构： [Gao-Qiang Liu] I;International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology, Changsha, Hunan 410004, China<&wdkj&>Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha, Hunan 410004, China<&wdkj&>Microbial Variety Creation Center, Hunan Provincial Laboratory of Yuelushan Seed Industry, Changsha 410004, China

关键词： Cordycepin biosynthesis;Cordyceps militaris;Multi-omics;Rotenone;Submerged fermentation

摘要： Cordycepin is the key pharmacologically active compound of Cordyceps militaris, and various fermentation strategies have been developed to increase cordycepin production. This study aimed to investigate the effect of rotenone on cordycepin biosynthesis in submerged fermentation of C. militaris, and also to explore its possible induction mechanisms via multi-omics analysis. Adding 5 mg/L rotenone significantly increased the cordycepin production by 316.09 %, along with mycelial growth inhibition and cell wall destruction. Moreover, transcriptomic analysis and metabolomic analysis revealed the accumulation of cordycepin was promoted by alterations in energy metabolism and amino acid metabolism pathways. Finally, the integration analysis of the two omics confirmed rotenone altered the nucleotide metabolism pathway toward adenosine and up-regulated the cordycepin synthesis genes (cns1-3) to convert adenosine to cordycepin. This work reports, for the first time, rotenone could act as an effective inducer of cordycepin synthesis. © 2023 Elsevier Ltd

语种： 英文

作者： 盘昱良;王晓玲;刘高强

期刊： 教育教学论坛,2022年(33):157-160 ISSN：1674-9324

作者机构： 中南林业科技大学 生命科学与技术学院,湖南 长沙 410211;[盘昱良; 刘高强; 王晓玲] 中南林业科技大学

关键词： 课程思政;生态文明;通识课;公共基础课

摘要： “生态文明通识教育”是高校实践生态育人的重要公共基础课,在其中融入课程思政是落实习近平总书记高校思想政治工作相关指示精神,践行“三全育人”的要求。而目前,高校对公共基础课的课程思政关注度不如专业课,教师开展思想政治教育的能力有待提升,部分大学生的思想成熟度不高等多种因素限制了“生态文明通识教育”课程思政的效果。因此,高校应更加重视公共基础课的课程思政建设,以加强顶层设计为起点,以提升教师能力为重点,以坚定学生理想信念为要点,不断完善课程思政体系,提升育人成效。

语种： 中文

作者： 曹小玉;刘高强;王晓玲

期刊： 高教学刊,2022年8(35):41-44 ISSN：2096-000X

作者机构： 中南林业科技大学 林学院,长沙 410004;中南林业科技大学 教务处,长沙 410004;中南林业科技大学 生命科学与技术学院,长沙 410004;[刘高强; 曹小玉; 王晓玲] 中南林业科技大学

关键词： 立德树人;三全育人;课程思政;协同创新;农林院校

摘要： 立德树人背景下农林院校课程思政协同创新是落实立德树人根本任务的重要保证；是巩固马克思主义在高校意识形态主导地位的根本所在；也是适应新时代高校课程体系建设趋势的必然要求。但目前农林院校课程思政协同创新中存在课程与思政功能严重脱节；课程思政协同不够；教师课程思政的意识与能力动力不足；课程思政协同创新机制不健全等问题。为使课程思政取得实效，必须充分发挥高校党委在课程思政协同创新中的主导作用；必须统筹推进课程思政创新主体协同发力；必须优化完善全课程、全过程、全员课程思政协同育人体系；必须积极探索课程思政协同创新培育、管理、平台和保障机制。

语种： 中文

作者： 尹媛媛;王晓玲;薛菲菲;黄佳;张少冰;...

期刊： 菌物学报,2021年40(9):2317-2329 ISSN：1672-6472

作者机构： [尹媛媛; 王晓玲; 薛菲菲; 黄佳; 张少冰; 刘高强] Hunan Provincial Key Laboratory of Forestry Biotechnology, International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology, Hunan, Changsha, 410004, China

关键词： 灵芝;三萜酸;生物合成;诱导机制;信号分子

摘要： 为探究9,10-环甲基十七烷酸(9,10-CMA)诱导灵芝三萜酸合成的相关信号机制,分析了NO信号的介导作用。结果表明,在NO信号分子介导下,9,10-CMA可有效地刺激灵芝菌丝体中三萜合成关键酶细胞色素CYP450和苯丙氨酸解氨酶(PAL)的活化以及三萜酸的合成。NO可作为灵芝菌丝体中CYP450产生和PAL活化的上游分子发挥作用。在9,10-CMA诱导下,通过荧光定量PCR分析了6个关键酶基因在灵芝三萜酸合成过程的动态表达。结果表明,在9,10-CMA诱导下与灵芝三萜酸合成相关的角鲨烯合成酶基因sqs、细胞色素P450单加氧酶CYP5150L8基因和3-羟基-3-甲基戊二酰辅酶A还原酶基因hmgr的上调最显著,提示这3个酶在三萜诱导过程中具有重要作用。

语种： 中文

作者： 郭璐;王雅葳;张少冰;刘帅;王晓玲;...

期刊： 菌物学报,2021年40(4):965-976 ISSN：1672-6472

作者机构： [郭璐; 王雅葳; 张少冰; 刘帅; 王晓玲; 刘高强] Hunan Provincial Key Laboratory of Forestry Biotechnology, International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology of Hunan Province, Central South University of Forestry & Technology, Hunan, Changsha, 410004, China

关键词： 忍冬桑黄;蛹虫草;共发酵;多糖

摘要： 忍冬桑黄和蛹虫草两种药用真菌均可产活性多糖,共发酵模式是产生新化合物或提高化合物含量或药效的潜在方式。本研究尝试用忍冬桑黄和蛹虫草共发酵联产真菌多糖,并对其共发酵所得的菌丝体多糖开展抗氧化活性试验。结果表明,当忍冬桑黄和蛹虫草预先分别发酵3d和1d后再同时接种共发酵,两菌可以较好地进行共生长,菌体总量和总多糖得率显著高于两菌单独进行发酵时的相应量。进一步对适宜两菌共发酵的培养基进行了优化,获得适宜两菌共发酵高产多糖的培养基组成为:可溶性淀粉30g/L、牛肉粉12g/L、磷酸氢二钾和硫酸镁各1.5g/L。共发酵菌丝体中多糖和黄酮含量均高于两菌单个菌体中的相应含量,但三萜含量和桑黄菌体中的三萜含量没有显著差异。抗氧化活性试验表明,共发酵菌体多糖、忍冬桑黄菌体多糖和蛹虫草菌体多糖对DPPH·自由基、羟基自由基、超氧阴离子自由基均具有清除作用,其中共发酵菌体多糖对3种自由基的清除作用显著强于两菌单个菌体多糖的清除效果。本研究表明忍冬桑黄和蛹虫草共发酵联产真菌多糖具有可行性。

语种： 中文

作者： Ma, Jiangshan;Yue, Huimin;Li, Hongqian;Zhang, Jing;Zhang, Yanghong;...

期刊： Biotechnology for Biofuels and Bioproducts,2021年14(1):1-15 ISSN：2731-3654

通讯作者： Gao-Qiang Liu

作者机构： [Zhang, Jing; Li, Hongqian; Gong, Si; Wang, Xiaoling; Liu, Gao-Qiang; Ma, Jiangshan; Zhang, Yanghong; Yue, Huimin] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Zhang, Jing; Li, Hongqian; Gong, Si; Wang, Xiaoling; Liu, Gao-Qiang; Ma, Jiangshan; Zhang, Yanghong; Yue, Huimin] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Liu, Gao-Qiang] H;Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, Hunan, P.R. China.;International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, Hunan, P.R. China.

关键词： Woody biomass;Fungal pretreatment;White-rot basidiomycete;Peniophora incarnate T-7;Submerged fermentation;Enzymatic saccharification;Quantitative proteomic analysis

摘要： Pretreatment is a critical step required for efficient conversion of woody biomass into biofuels and platform chemicals. Fungal pretreatment is regarded as one of the most promising technology for woody biomass conversion but remains challenging for industrial application. The exploration of potential fungus strain with high efficient delignification and less processing time for woody biomass pretreatment will be valuable for development of biorefinery industry. Here, a newly isolated white-rot basidiomycete Peniophora incarnate T-7 was employed for poplar wood pretreatment. The chemical component analysis showed that cellulose, hemicellulose and lignin from poplar wood declined by 16%, 48% and 70%, respectively, after 7 days submerged fermentation by P. incarnate T-7. Enzymatic saccharification analysis revealed that the maximum yields of glucose and xylose from 7 days of P. incarnate T-7 treated poplar wood reached 33.4% and 27.6%, respectively, both of which were enhanced by sevenfold relative to the untreated group. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffraction (XRD) and pyrolysis gas chromatography–mass spectrometry (Py-GC/MS) characterization confirmed that lignocellulosic structure of poplar wood was largely broken by P. incarnate T-7, including delignification and de-crystalline of cellulose. Meanwhile, lignin component of poplar wood was selectively degraded by P. incarnate T-7, and G-type unit of lignin was preferentially attacked by the strain. Furthermore, quantitative proteomic analysis revealed that a considerable amount of lignocellulolytic enzymes were detected in the secretory proteins of P. incarnate T-7, especially with high abundance of lignin-degrading enzymes and hemicellulases. Combination of quantitative proteomic with transcriptomic analysis results showed that most of those lignocellulolytic enzymes were highly upregulated on poplar wood substrate compared to glucose substrate. This study showed that P. incarnate T-7 could selectively delignify poplar wood by submerged fermentation with short time of 7 days, which greatly improved its enzymatic saccharification efficiency. Our results suggested that P. incarnate T-7 might be a promising candidate for industrial woody biomass pretreatment.

语种： 英文

作者： Wu, Qiang;Li, Yong;Peng, Kuan;Wang, Xiao-Ling*;Ding, Zhongyang;...

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019年67(29):8149-8159 ISSN：0021-8561

通讯作者： Wang, Xiao-Ling;Liu, Gao-Qiang

作者机构： [Li, Yong; Liu, Gao-Qiang; Peng, Kuan; Wang, Xiao-Ling; Wu, Qiang; Liu, GQ] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Li, Yong; Liu, Gao-Qiang; Peng, Kuan; Wang, Xiao-Ling; Wu, Qiang; Liu, GQ] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.;[Wu, Qiang] Shaoyang Univ, Coll Food & Chem Engn, Shaoyang 422000, Peoples R China.;[Ding, Zhongyang; Liu, Liming] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Jiangsu, Peoples R China.;[Xu, Peng] Univ Maryland Baltimore Cty, Coll Chem Biochem & Environm Engn, Baltimore, MD 21201 USA.

通讯机构： [Wang, XL; Liu, GQ] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.

关键词： Endothelial cells;Hydrogen bonds;Nitric oxide;Peptides;Ace inhibitory peptides;Anti-hypertension;Ganoderma Lucidum;Molecular dynamics simulations;Submerged fermentation;Molecular dynamics;antihypertensive agent;dipeptidyl carboxypeptidase;dipeptidyl carboxypeptidase inhibitor;peptide;amino acid sequence;cell line;chemistry;Ganoderma lucidum;human;isolation and purification;kinetics;mass spectrometry;metabolism;molecular dynamics;mycelium;peptide mapping;Amino Acid Sequence;Angiotensin-Converting Enzyme Inhibitors;Antihypertensive Agents;Cell Line;Humans;Kinetics;Mass Spectrometry;Molecular Dynamics Simulation;Mycelium;Peptide Mapping;Peptides;Peptidyl-Dipeptidase A;Reishi

摘要： Ganoderma lucidum (G. lucidum) has been widely used in Asia to treat hypertension, but the active substances responsible for its antihypertensive effects remain unclear. Using the well-established angiotensin I-converting enzyme (ACE) as a target, we identified three ACE inhibitory peptides (ACEIPs), Gln-Leu-Val-Pro (QLVP), Gln-Asp-Val-Leu (QDVL), and Gln-Leu-Asp-Leu (QLDL), which account for the antihypertensive activity of G. lucidum. Notably, QLVP worked in a mixed-type manner against ACE with an IC50 value of 127.9 μmol/L. Molecular dynamics simulation suggested that the potent charge energy of QLVP, which interacted with Gln242 and Lys472 of ACE via a hydrogen bond and a salt bridge, potentially contributed to ACE inhibitory activity. Moreover, QLVP markedly activated angiotensin I-mediated phosphorylation of endothelial nitric oxide synthase in human umbilical vein endothelial cells and partly reduced mRNA and protein expression of the vasoconstrictor factor endothelin-1. This is the first report of the antihypertensive activity of small ACEIPs originating from G. lucidum mycelia, paving the way for the possible application of these peptides as potent drug candidates for treating hypertension. © 2019 American Chemical Society.

语种： 英文

作者： Wang, Xiao-Ling;Ding, Zhong-Yang;Zhao, Yan;Liu, Gao-Qiang*;Zhou, Guo-Ying

期刊： INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS,2017年19(5):419-431 ISSN：1521-9437

通讯作者： Liu, Gao-Qiang

作者机构： [Liu, Gao-Qiang; Zhou, Guo-Ying; Zhao, Yan; Wang, Xiao-Ling] Cent South Univ Forestry Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha, Peoples R China.;[Ding, Zhong-Yang] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi, Peoples R China.;[Liu, Gao-Qiang; Zhou, Guo-Ying] Cent South Univ Forestry Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha, Hunan, Peoples R China.;[Liu, Gao-Qiang] 498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Liu, Gao-Qiang] 4;498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

关键词： ganoderic acid;Ganoderma lucidum extract;triterpene;unclassified drug;antineoplastic agent;ganoderic acid T;lanosterol;triterpene;antineoplastic activity;Article;batch fermentation;biomass production;chemical analysis;cytotoxicity;fungal biomass;Ganoderma lucidum;human;human cell;in vitro study;mathematical model;mortality;MTT assay;mycelial growth;nonhuman;response surface method;scale up;stirred reactor;submerged fermentation;validation process;analogs and derivatives;bioreactor;chemistry;fermentation;Ganoderma lucidum;isolation and purification;temperature;traditional medicine;tumor cell line;Antineoplastic Agents;Bioreactors;Cell Line, Tumor;Fermentation;Humans;Lanosterol;Medicine, Traditional;Reishi;Temperature;Triterpenes

摘要： Triterpene acids are among the major bioactive constituents of Ganoderma lucidum. However, submerged fermentation techniques for isolating triterpene acids from G. lucidum have not been optimized for commercial use, and the antitumor activity of the mycelial triterpene acids needs to be further proven. The aim of this work was to optimize the conditions for G. lucidum culture with respect to triterpene acid production, scaling up the process, and examining the in vitro antitumor activity of mycelial triterpene acids. The key conditions (i.e., initial pH, fermentation temperature, and rotation speed) were optimized using response surface methodology, and the in vitro antitumor activity was evaluated using the MTT method. The optimum key fermentation conditions for triterpene acid production were pH 6.0; rotation speed, 161.9 rpm; and temperature, 30.1°C, resulting in a triterpene acid yield of 291.0 mg/L in the validation experiment in a 5-L stirred bioreactor; this yield represented a 70.8% increase in titer compared with the nonoptimized conditions. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and a triterpene productivity of 47.9 mg/L/day was achieved, which is, to our knowledge, the highest reported in the large-scale fermentation of G. lucidum. In addition, the mycelial triterpene acids were found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro. Chemical analysis showed that the key active triterpene acid compounds, ganoderic acids T and Me, predominated in the extract, at 69.2 and 41.6 mg/g, respectively. Thus, this work develops a simple and feasible batch fermentation technique for the large-scale production of antitumor triterpene acids from G. lucidum. © 2017 Begell House, Inc.

语种： 英文

作者： 王晓玲;周广乙;刘高强

期刊： 菌物学报,2016年35(10):1250-1257 ISSN：1672-6472

作者机构： [王晓玲; 周广乙; 刘高强] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[王晓玲; 周广乙; 刘高强] 林业生物技术湖南省重点实验室, 湖南, 长沙, 410004

关键词： 药用真菌;液体发酵;虫草酸;活性成分;发酵动力学

摘要： 多糖和D-甘露醇是蛹虫草的重要药理活性成分。本文开展了蛹虫草在分批发酵过程中同时生产多糖和D-甘露醇的发酵动力学研究。利用Sigmoid函数构建了蛹虫草菌丝生长、糖基质消耗、多糖和D-甘露醇的非结构动力学模型,并根据Boltzmann方程拟合求解出各模型参数。结果显示,各模型的实测值和预测值拟合度较好。蛹虫草比生长速率在第1.0天达到最大值(μ_(max))1.244d~(-1);底物葡萄糖的比消耗速率在第0.6天达到最大值(q_(S, max))2.163d~(-1);多糖比合成速率在第2.0天达到最大值(q_(P, max))51.852mg/(g?d);D-甘露醇比合成速率在第0.99天左右达到最大值(q_(D, max))37.963mg/(g?d)。蛹虫草多糖的形成与菌丝细胞的生长呈现部分生长关联型,而D-甘露醇的形成与细胞生长呈现生长关联型关系。研究结果为利用分批发酵规模化同时发酵生产蛹虫草多糖和D-甘露醇提供了理论依据。

语种： 中文

作者： 张冬雪;熊婷;王晓玲;刘高强

期刊： 菌物学报,2016年35(9):1139-1150 ISSN：1672-6472

作者机构： [张冬雪; 熊婷; 王晓玲; 刘高强] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[张冬雪; 熊婷; 王晓玲; 刘高强] 南方林业生态应用技术国家工程实验室, 湖南, 长沙, 410004

关键词： 灵芝;多糖;Box‐Behnken设计;超声波‐酶水解法

摘要： 比较了热水回流法、热水‐酶回流法、超声波法以及超声波‐酶水解法等4种提取方法对灵芝菌丝体中β‐葡聚糖提取得率的影响。结果表明,采用超声波‐酶水解法所得β‐葡聚糖的得率最大,影响提取的关键因素为超声波功率(A),水料比(B),pH(C)和加酶量(D)。进一步采用Box‐Behnken设计及响应面分析法对这4个因素进行了优化。结果显示,因素A、B、C对β‐葡聚糖提取得率的影响达到极显著水平(P<0.01),但因素D和所有因素之间的交互作用并不显著(P>0.05)。通过回归拟合,建立了预测灵芝β‐葡聚糖提取的多项式模型。经响应面最优分析,获得4个因素的最佳水平为:超声波功率275.54W,水料比25.62:1(V/M,mL/g),pH 7.17,加酶量1.05%。经实际试验验证,在该条件下,灵芝菌丝体β‐葡聚糖得率可达5.50mg/g。

语种： 中文

作者： 刘高强;王晓玲;杨海龙

作者机构： [刘高强; 王晓玲; 杨海龙] College of Life Science and Technology,Central South University of Forestry and Technology;[刘高强; 王晓玲; 杨海龙] College of Life and Environmental Science,Wenzhou University

会议名称： 中国菌物学会2016年学术年会

会议时间： 2016-08-19

会议地点： 福州

关键词： Ganoderma lucidum;Triterpene acids;Fermentation;Process control;Biochemical engineering;Bioprocesses

摘要： BACKGROUND:Triterpenes(especially triterpene acids) is one of the major physiologically active components of Ganoderma lucidum.However,submerged fermentation techniques of triterpenes is not optimized

语种： 英文

作者： Wang, Xiao-Ling;Yang, Hailong;Liu, Gao-Qiang*

期刊： JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY,2016年91(12):2974-2982 ISSN：0268-2575

通讯作者： Liu, Gao-Qiang

作者机构： [Liu, Gao-Qiang; Wang, Xiao-Ling] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Coll Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yang, Hailong] Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Peoples R China.;[Liu, Gao-Qiang] 498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Liu, Gao-Qiang] 4;498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

关键词： Biochemical engineering;Feeding;Fermentation;pH;Process control;Acid concentrations;Active components;Bioprocesses;Ganoderma Lucidum;PH control strategies;Submerged cultures;Submerged fermentation;Triterpenes;pH effects;acid;palmitic acid;sugar;triterpene;Article;batch fermentation;biomass;biosynthesis;Catharsius molossus;comparative study;controlled study;feeding behavior;fermentation;Ganoderma lucidum;insect;kinetics;mycelial growth;nonhuman;pH;sugar intake

摘要： BACKGROUND: Triterpenes (especially triterpene acids) are some of the major physiologically active components of Ganoderma lucidum. However, submerged fermentation techniques for triterpenes have not been optimized for commercial use, and the total triterpene yield is still very low. The aim of this work was to develop a two-stage pH-control strategy integrated with the use of an ether extract from the medicinal insect, Catharsius molossus (EECM), as a stimulus for enhanced G. lucidum triterpene acid production. RESULTS: Triterpene acid production of 302.76 mg L−1 was obtained in G. lucidum cultured with the EECM; this production was higher by 60% than that of the control cultures not fed the EECM. Based on the EECM feeding method, a new two-stage pH-control strategy was integrated, resulting in triterpene acid yields of 361.47 mg L−1. Subsequent studies optimized the interaction between EECM feeding and two-stage pH-control strategies, which resulted in an optimal EECM concentration of 185.89 mg L−1 and optimal pH values of 5.07 in the first 72 h incubation and 4.37 after 72 h, with a maximum triterpene acid concentration of 449.37 mg L−1. This represented a 137% increase compared with that of the control cultures. Furthermore, when a 150 L fermenter was used under these conditions, a triterpene acid concentration of 431.83 mg L−1 was achieved. CONCLUSIONS: The enhanced production of triterpene acid in submerged cultures of G. lucidum was achieved using an EECM feeding stimulus integrated with a two-stage pH-control strategy at large scale. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry

语种： 英文

作者： 蒋程;王硕;王宝庆;张建华;赵天阳

期刊： 电工技术学报,2016年31(10):193-206 ISSN：1000-6753

通讯作者： Jiang, Cheng(jc_2002@163.com)

作者机构： 国网北京房山供电公司北京 100031;华北电力大学电气与电子工程学院北京 102206;[蒋程] 蒋程;[赵天阳] 赵天阳;[王硕] 王硕

通讯机构： State Grid Beijing Fangshan Electric Power Company, Beijing, China

关键词： 概率可靠性评估;电力系统;拉丁超立方采样;风电;Cholesky分解

摘要： 基于随机抽样的蒙特卡罗方法(MC_RS)由于原理简单、易于实现,常用于电力系统的可靠性评估,但在大规模风电接入,特别是以单个小容量的机组接入的情况下会存在样本容量大、效率低等不足。因此提出使用基于拉丁超立方采样的蒙特卡罗(MC_LHS)方法来解决含风电的电力系统概率可靠性评估问题,此方法由于使用了拉丁超立方采样,能有效地改善样本值对输入随机变量的分布空间的覆盖程度和使用Cholesky分解来降低输入变量之间的相关性系数,从而提高了采样效率、增加收敛速度和提高评估准确度。把提出的MC_LHS方法应用到改进IEEE-RTS79算例中,并与常规MC_RS进行比较,结果验证了该方法的有效性。

语种： 中文

作者： 曹静;王晓玲;刘高强;周国英

期刊： 菌物学报,2016年35(1):104-113 ISSN：1672-6472

作者机构： 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004;中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[刘高强] 南方林业生态应用技术国家工程实验室, 湖南, 长沙, 410004;[曹静; 王晓玲; 周国英] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004;[刘高强] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室

关键词： 灵芝;β-葡聚糖;热水提取;Box-Behnken设计

摘要： 采用乙醇-酶预处理体系,结合水提法较系统地研究了灵芝子实体中β-葡聚糖的提取技术。结果表明,乙醇-酶体系预处理的关键参数为:乙醇质量分数60%(V/V),加酶量1.5%(M/V,g/mL)、酶解温度45℃、酶解pH8.0;在乙醇-酶体系预处理的基础上,进一步采用单因素试验和Box-Benhnken试验设计与响应面分析法对灵芝子实体中β-葡聚糖的热水提取工艺进行了优化。结果显示水提温度、提取时间、水料比3个因素及水提温度与水料比二者的交互作用对β-葡聚糖的提取有显著影响。经优化后获得3个核心因素的最佳水平为:提取温度80℃、提取时间2.5h、水料比35:1。在乙醇-酶预处理结合水提取条件下,灵芝子实体中β-葡聚糖的提取得率可达0.412mg/g,是传统无水乙醇回流预处理结合水提法提取β-葡聚糖得率的2.1倍。本研究为灵芝β-葡聚糖的进一步提取放大试验奠定了技术基础。

语种： 中文

作者： Wang, Xiao-Ling;Ding, Zhong-Yang;Liu, Gao-Qiang*;Yang, Hailong;Zhou, Guo-Ying

期刊： Molecules,2016年21(10):1395 ISSN：1420-3049

通讯作者： Liu, Gao-Qiang

作者机构： [Liu, Gao-Qiang; Zhou, Guo-Ying; Wang, Xiao-Ling] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Liu, Gao-Qiang; Zhou, Guo-Ying; Wang, Xiao-Ling] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.;[Ding, Zhong-Yang] Jiangnan Univ, Natl Engn Lab Cereal Fermentat Technol, Wuxi 214122, Peoples R China.;[Yang, Hailong] Wenzhou Univ, Coll Life & Environm Sci, Wenzhou 325035, Peoples R China.

通讯机构： [Liu, Gao-Qiang] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.

会议名称： 中国菌物学会2018年学术年会

会议时间： 2018-08-11

会议地点： 中国山东泰安

会议论文集名称： 中国菌物学会2018年学术年会论文汇编

关键词： antineoplastic agent;ganoderic acid;ganoderic acid T;lanosterol;triterpene;analogs and derivatives;animal;bioreactor;cell proliferation;cell survival;chemistry;dose response;drug effects;drug screening;fermentation;Ganoderma;growth, development and aging;human;metabolism;microbiology;mouse;mycelium;sarcoma 180;tumor cell line;Animals;Antineoplastic Agents;Bioreactors;Cell Line, Tumor;Cell Proliferation;Cell Survival;Dose-Response Relationship, Drug;Fermentation;Ganoderma;Humans;Lanosterol;Mice;Mycelium;Sarcoma 180;Triterpenes;Xenograft Model Antitumor Assays

摘要： Triterpene acids (TAs) are the major bioactive constituents in the medicinal fungus Ganoderma lingzhi. However, fermentative production of TAs has not been optimized for commercial use, and whether the TAs isolated from G. lingzhi submerged culture mycelia possess antitumor activity needs to be further proven. In this study, enhanced TA yield and productivity were attained with G. lingzhi using response surface methodology. The interactions of three variables were studied using a Box-Benhnken design, namely initial pH, dissolved oxygen (DO) and fermentation temperature. The optimum conditions were an initial pH of 5.9, 20.0% DO and 28.6 XC. These conditions resulted in a TA yield of 308.1 mg/L in a 5-L stirred bioreactor. Furthermore, the optimized conditions were then successfully scaled up to a production scale of 200 L, and maximum TA production and productivity of 295.3 mg/L and 49.2 mg/L/day were achieved, which represented 80.9% and 111.5% increases, respectively, compared with the non-optimized conditions. Additionally, the triterpene acid extract (TAE) from G. lingzhi mycelia was found to be cytotoxic to the SMMC-7721 and SW620 cell lines in vitro, and the TAE exhibited dose-dependent antitumor activity against the solid tumor sarcoma 180 in vivo. Chemical analysis revealed that the key active triterpene compounds, ganoderic acid T and ganoderic acid Me, predominated in the extract. © 2016 by the authors.

语种： 英文

作者： 王晓玲;万芳芳;刘高强

期刊： 微生物学通报,2016年43(5):1019-1026 ISSN：0253-2654

作者机构： [王晓玲; 万芳芳; 刘高强] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[王晓玲; 万芳芳; 刘高强] 南方林业生态应用技术国家工程实验室, 湖南, 长沙, 410004

关键词： 菌株筛选;甲壳素脱乙酰酶;变色圈法;层生镰刀菌

摘要： 【目的】甲壳素脱乙酰酶(CDA)是将天然甲壳素生物转化为可商品化利用的壳聚糖的关键酶。本文旨在从湘江河岸的土壤中筛选可高产CDA的新菌株。【方法】以甲壳素为唯一碳源,利用4'-硝基乙酰苯胺为显色剂,通过变色圈法进行产CDA菌株初筛,产酶活性分析复筛;通过形态学和ITS区序列特征对菌株进行鉴定。【结果】从湘江(长沙段)河岸边的土壤中分离出的117株菌株中筛选到可产CDA的菌株30株,其中4株具有较强产CDA的能力。进一步经发酵产酶分析验证,菌株A1具有较强的产CDA能力,其胞外CDA酶活高达13.21 U/mL。结合形态学和ITS区序列特征,菌株A1初步鉴定为层生镰孢菌。【结论】从湘江河岸边的土壤中筛选到可高产CDA的菌株A1,具有较好的开发应用前景。

语种： 中文

作者： 张冬雪;王晓玲;刘高强

期刊： 菌物学报,2016年35(6):750-759 ISSN：1672-6472

作者机构： [张冬雪; 王晓玲; 刘高强] 中南林业科技大学, 经济林培育与保护湖南省部共建教育部重点实验室;[张冬雪; 王晓玲; 刘高强] 南方林业生态应用技术国家工程实验室, 湖南, 长沙, 410004

关键词： 灵芝;β-葡聚糖;Box-Behnken 设计;液体发酵

摘要： 灵芝β-葡聚糖是灵芝多糖中生物活性较高的一类多糖。通过单因素试验确定影响灵芝液体发酵β-葡聚糖的3个关键参数为:可溶性淀粉(A)40g/L,蛋白胨(B)8g/L,K_2HPO_4(C)1.5g/L。在此基础上,采用Box-Behnken设计及响应面分析法对各参数及其交互作用进行了研究。结果显示,3因素及AB、AC的交互作用对β-葡聚糖得率的影响均为极显著水平(P<0.01)。建立的预测灵芝β-葡聚糖发酵的多项式模型为:Y_(β-葡聚糖)=8.68+0.22A-0.13B+0.096C+0.13AB+0.16AC+ 0.045BC-1.17A~2-0.81B~2-1.06C~2。经响应面最优分析,获得3个关键因素的最佳水平为:可溶性淀粉40.47g/L,蛋白胨7.86g/L, K_2HPO_4 1.53g/L,在该条件下,灵芝菌体β-葡聚糖得率可达8.68mg/g。

语种： 中文

作者： 黎李平;张容容;雷含珺;王晓玲

期刊： 食药用菌,2016年(5):294-299 ISSN：2095-0934

作者机构： 中南林业科技大学生命科学与技术学院;[雷含珺; 张容容; 黎李平; 王晓玲] 中南林业科技大学

关键词： 灵芝;灵芝三萜;液体发酵;发酵动力学;研究进展

摘要： 灵芝三萜是灵芝的重要药效成分，液体发酵技术是大量获取灵芝三萜的重要途径。概述灵芝发酵产三萜的菌株筛选，培养基优化和发酵技术，三萜液体发酵动力学，三萜生物合成的相关酶和基因表达调控等，综述灵芝三萜液体发酵的最新研究进展，并对今后的相关研究进行了展望。

语种： 中文