期刊论文

中文

菌物学报

1672-6472

2016

35

6

750-759

10.13346/j.mycosystema.150082

(2012-4-11):国家“948”计划项目 (NCET-13-1046):教育部“新世纪优秀人才支持计划”基金 (2015JJ1025):湖南省自然科学***基金 (14K112):湖南省高校创新平台开放基金 (2012-4-11):Supported by National "948" Project,State Forestry Bureau (NCET-13-1046):Program for New Century Excellent Talents in University (2015JJ1025):Hunan Provincial Natural Science Foundation of China for Distinguished Young Scholars (14K112):The Research Foundation of Education Bureau of Hunan Province,China

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灵芝β-葡聚糖是灵芝多糖中生物活性较高的一类多糖。通过单因素试验确定影响灵芝液体发酵β-葡聚糖的3个关键参数为:可溶性淀粉(A)40g/L,蛋白胨(B)8g/L,K_2HPO_4(C)1.5g/L。在此基础上,采用Box-Behnken设计及响应面分析法对各参数及其交互作用进行了研究。结果显示,3因素及AB、AC的交互作用对β-葡聚糖得率的影响均为极显著水平(P

β-glucan is one of key bioactive components of Ganoderma lingzhi polysaccharides. Single factor experiment revealed that the key parameters affecting β-glucan yield in Ganoderma lingzhi submerged fermentation was as follows: starch (A) 40g/L, peptone (B) 8g/L, K_2HPO_4 (C) 1.5g/L. Based on the results, Box-Benhnken design combined with response surface methodology was applied to optimize the levels of three key parameters and study their interaction on β-glucan production. The results showed that the three factors and the interaction of AB a...