期刊论文

Analysis of Key Active Compounds and Fermentation Technology of Ophiocordyceps sinensis Mycelium

Liu, Gaoqiang(gaoliuedu@163.com)

中文

中国食品学报

1009-7848

2016

16

1

91-98

10.16429/j.1009-7848.2016.01.013

NCET-13-1046:新世纪优秀人才支持计划 2012-4-11:引进国际先进农业科学技术计划计划) 10K081:湖南省高等学校创新平台开放基金 12K069:湖南省高等学校创新平台开放基金

本校为第一且通讯机构

研究了重大害虫松毛虫作为冬虫夏草主要氮源基质的深层发酵技术,并对所得冬虫夏草菌体中的关键功效成分进行分析。结果表明,最适宜冬虫夏草菌体生长的松毛虫基质为松毛虫幼虫冻干粉,补充碳源为玉米粉,补充氮源为(NH_4)_2SO_4,无机盐为KH_2PO_4。采用均匀设计优化了培养基组成,获得适宜该菌株液体发酵的最佳培养基为:松毛虫冻干粉13.0 g/L、玉米粉38.0 g/L、(NH_4)_2SO_4 3.0 g/L、葡萄糖3.0 g/L、KH_2PO_4 1.4 g/L、蛋白胨1.0 g/L、MgSO_4·7H_2O 0.75 g/L。在此条件下,冬虫夏草菌体生物量可达8.68 g/L。功效成分分析表明,所得冬虫夏草菌体中多糖和腺苷的含量显著高于野生冬虫夏草中对应两成分的...

Submerged fermentation of Ophiocordyceps sinensis using a culture medium with key insect pest, Den-drolimus punctantus as main nitrogen source, and analysis of key active compounds of the mycelia were investigated. The results shows that the freeze-dried powder of D. punctantus was suitable for cell growth of O. sinensis, and the supple- mentary carbon source, nitrogen source and mineral element were corn flour, (NH4)2SO4 and KH2PO4, respectively. The fermentation medium was further optimized by Uniform Design experiment, and a optimal fermenta...