作者机构：
[Xin Y. Jiang] Cent S Univ Forestry & Technol, Coll Mat Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Sven G. Sommer; Jin M. Triolo] Univ So Denmark, Fac Engn, Inst Chem Engn Biotechnol & Environm Technol, DK-5230 Odense M, Denmark.;[Martin R. Weisbjerg] Aarhus Univ, Fac Agr Sci, Dept Anim Hlth & Biosci, DK-8830 Tjele, Denmark.;[Moller, Henrik B.] Aarhus Univ, Fac Agr Sci, Dept Biosyst Engn, Res Ctr Foulum, DK-8830 Tjele, Denmark.
通讯机构：
[Triolo, JM] Univ So Denmark, Fac Engn, Inst Chem Engn Biotechnol & Environm Technol, Niels Bohrs Alle 1, DK-5230 Odense M, Denmark.
关键词：
Biogas;Digestibility;Lignin;Lignocellulose;Predicting methane production potential
摘要：
We examined the influence of fibrous fractions of biomass on biochemical methane potential (BMP) with the objective of developing an economical and easy-to-use statistical model to predict BMP, and hence the biodegradability of organic material (BD) for biogas production. The model was developed either for energy crops (grass, maize, and straw) or for animal manures, or as a combined model for these two biomass groups. It was found that lignin concentration in volatile solids (VS) was the strongest predictor of BMP for all the biomass samples. The square of the sample correlation coefficient (R(2)) from the BMP versus lignin was 0.908 (p < 0.0001), 0.763 (p < 0.001) and 0.883 (p < 0.001) for animal manure, energy crops and the combined model, respectively. Validation of the combined model was carried out using 65 datasets from the literature. (C) 2011 Elsevier Ltd. All rights reserved.
作者机构：
[Guoli Shen; Ronghua Yang; Ruqin Yu; Jianhui Jiang; Xia Chu; Ting Deng; Jishan Li] State Key Laboratory of Chem/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China;[Ting Deng] Institute of Biological and Environmental Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, China
作者机构：
[Chengjun Zhou; Jingquan Han; Qinglin Wu] School of Renewable Natural Resources, Louisiana State University AgCenter, Baton Rouge, LA 70803, United States;[Fangyang Liu] Department of Physics and Astronomy, Louisiana State University, Baton Rouge, LA 70803, United States;[Yiqiang Wu] College of Materials Science and Engineering, Central South Forestry University of Science and Technology, Changsha, China
通讯机构：
[Wu, QL] Louisiana State Univ AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA.
关键词：
carbon budget;ice retreat;intact ecosystems;land use change;permafrost
摘要：
With a pace of about twice the observed rate of global warming, the temperature on the Qinghai-Tibetan Plateau (Earth's 'third pole') has increased by 0.2 degrees C per decade over the past 50 years, which results in significant permafrost thawing and glacier retreat. Our review suggested that warming enhanced net primary production and soil respiration, decreased methane (CH(4)) emissions from wetlands and increased CH(4) consumption of meadows, but might increase CH(4) emissions from lakes. Warming-induced permafrost thawing and glaciers melting would also result in substantial emission of old carbon dioxide (CO(2)) and CH(4). Nitrous oxide (N(2)O) emission was not stimulated by warming itself, but might be slightly enhanced by wetting. However, there are many uncertainties in such biogeochemical cycles under climate change. Human activities (e.g. grazing, land cover changes) further modified the biogeochemical cycles and amplified such uncertainties on the plateau. If the projected warming and wetting continues, the future biogeochemical cycles will be more complicated. So facing research in this field is an ongoing challenge of integrating field observations with process-based ecosystem models to predict the impacts of future climate change and human activities at various temporal and spatial scales. To reduce the uncertainties and to improve the precision of the predictions of the impacts of climate change and human activities on biogeochemical cycles, efforts should focus on conducting more field observation studies, integrating data within improved models, and developing new knowledge about coupling among carbon, nitrogen, and phosphorus biogeochemical cycles as well as about the role of microbes in these cycles.
作者：
Jiang, Xinyuan;Sommer, Sven G.;Christensen, Knud V.
期刊：
ENERGY POLICY,2011年39(10):6073-6081 ISSN：0301-4215
通讯作者：
Jiang, X.(jxycsfu@126.com)
作者机构：
[Jiang, Xinyuan ] School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan province 410004, China;[ Sommer, Sven G. ; Christensen, Knud V. ] Institute of Chemical Engineering, Biotechnology and Environmental Technology, Faculty of Engineering, University of Southern Denmark, Niels Bohrs Alle 1, DK-5230 Odense M, Denmark
通讯机构：
[Jiang, XY] Cent S Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Biogas plants - China - Economic development - Engineering equipments - Environmental policy - Follow-up service - Future perspectives - Long-term follow-up - Plant construction - Rural environment - Service systems - Sustainable energy
摘要：
This article presents an overview of the development and future perspectives of the Chinese biogas industry. The development of the industry has the potential to improve the rural environment and produce significant amounts of sustainable energy for China. Barriers to the development are the relatively weak environmental policies, imperfect financial policies and lack of long-term follow-up services. The rapid economic development of China has also seen a development in the scales of biogas plants constructed. Although the technology has been improved, this review has identified problems in the construction and operation of Chinese biogas plants, particularly in the efficiency of household systems. All levels of China's government acknowledge this and recent biogas projects have more focus on quality and less on the quantity. The intention is to gradually introduce stricter environmental policies, to provide better service systems, improve the financial policies that support the construction and follow-up service of biogas projects, promote the use of standardized engineering equipment and materials and standards for plant construction and production. This will promote the development of biogas projects at various scales further, and reduce the dependency on fossil fuels and emissions of greenhouse gases. (C) 2011 Elsevier Ltd. All rights reserved.
作者机构：
[He, Meng-Dong] Institute of Mathematics and Physics, Central South University of Forestry and Technology, Changsha, 410004, China;[Wang, Ling-Ling; Liu, Jian-Qiang; Zou, B S; Huang, Wei-Qing] School of Physics and Microelectronic, Hunan University, Changsha 410082, China;[Liu, Jian-Qiang; Wang, Dianyuan] College of Science, Jiujiang University, Jiujiang, 332005, China;[Wen, Shuangchun] School of Computer and Communication, Hunan University, Changsha 410082, China
摘要：
Sources of methane (CH4 ) become highly variable for countries undergoing a heightened period of development due to both human activity and climate change. An urgent need therefore exists to budget key sources of CH4 , such as wetlands (rice paddies and natural wetlands) and lakes (including reservoirs and ponds), which are sensitive to these changes. For this study, references in relation to CH4 emissions from rice paddies, natural wetlands, and lakes in China were first reviewed and then reestimated based on the review itself. Total emissions from the three CH4 sources were 11.25 Tg CH4 yr(-1) (ranging from 7.98 to 15.16 Tg CH4 yr(-1) ). Among the emissions, 8.11 Tg CH4 yr(-1) (ranging from 5.20 to 11.36 Tg CH4 yr(-1) ) derived from rice paddies, 2.69 Tg CH4 yr(-1) (ranging from 2.46 to 3.20 Tg CH4 yr(-1) ) from natural wetlands, and 0.46 Tg CH4 yr(-1) (ranging from 0.33 to 0.59 Tg CH4 yr(-1) ) from lakes (including reservoirs and ponds). Plentiful water and warm conditions, as well as its large rice paddy area make rice paddies in southeastern China the greatest overall source of CH4 , accounting for approximately 55% of total paddy emissions. Natural wetland estimates were slightly higher than the other estimates owing to the higher CH4 emissions recorded within Qinghai-Tibetan Plateau peatlands. Total CH4 emissions from lakes were estimated for the first time by this study, with three quarters from the littoral zone and one quarter from lake surfaces. Rice paddies, natural wetlands, and lakes are not constant sources of CH4 , but decreasing ones influenced by anthropogenic activity and climate change. A new progress-based model used in conjunction with more observations through model-data fusion approach could help obtain better estimates and insights with regard to CH4 emissions deriving from wetlands and lakes in China.
摘要：
Understanding the dynamics and underlying mechanism of carbon exchange between terrestrial ecosystems and the atmosphere is one of the key issues in global change research. In this study, we quantified the carbon fluxes in different terrestrial ecosystems in China, and analyzed their spatial variation and environmental drivers based on the long-term observation data of ChinaFLUX sites and the published data from other flux sites in China. The results indicate that gross ecosystem productivity (GEP), ecosystem respiration (ER), and net ecosystem productivity (NEP) of terrestrial ecosystems in China showed a significantly latitudinal pattern, declining linearly with the increase of latitude. However, GEP, ER, and NEP did not present a clear longitudinal pattern. The carbon sink functional areas of terrestrial ecosystems in China were mainly located in the subtropical and temperate forests, coastal wetlands in eastern China, the temperate meadow steppe in the northeast China, and the alpine meadow in eastern edge of Qinghai-Tibetan Plateau. The forest ecosystems had stronger carbon sink than grassland ecosystems. The spatial patterns of GEP and ER in China were mainly determined by mean annual precipitation (MAP) and mean annual temperature (MAT), whereas the spatial variation in NEP was largely explained by MAT. The combined effects of MAT and MAP explained 79%, 62%, and 66% of the spatial variations in GEP, ER, and NEP, respectively. The GEP, ER, and NEP in different ecosystems in China exhibited 'positive coupling correlation' in their spatial patterns. Both ER and NEP were significantly correlated with GEP, with 68% of the per-unit GEP contributed to ER and 29% to NEP. MAT and MAP affected the spatial patterns of ER and NEP mainly by their direct effects on the spatial pattern of GEP.
期刊：
ACS Sustainable Chemistry and Engineering,2015年3(5):821-832 ISSN：2168-0485
通讯作者：
Wu, QL
作者机构：
[Lee, Sunyoung] Department of Forest Products, Korea Forest Research Institute, Seoul, Korea, Republic of;[Qing, Yan; Wu, Yiqiang] College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, China;[Song, Kunlin; Wu, Qinglin; Li, Mei-Chun] School of Renewable Natural Resources, Louisiana State University AgCenter, Baton Rouge;[Song, Kunlin; Wu, Qinglin; Li, Mei-Chun] LA, United States;LA, United States
通讯机构：
[Wu, QL] Louisiana State Univ, AgCenter, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA.
关键词：
Cellulose nanofibers;Cellulose nanocrystals;Structure;Rheological properties;Aspect ratio
作者机构：
[Sabo, Ronald; Qing, Yan; Agarwal, Umesh; Zhu, J. Y.; Cai, Zhiyong] USDA Forest Service, Forest Products Laboratory, Madison, WI 53726-2398, United States;[Qing, Yan; Wu, Yiqiang] School of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
通讯机构：
[Sabo, R] US Forest Serv, USDA, Forest Prod Lab, Madison, WI 53726 USA.
关键词：
Cellulose nanofibrils;Refining;Microfluidization;Enzymatic and chemical pretreatments
摘要：
Various cellulose nanofibrils (CNFs) created by refining and microfluidization, in combination with enzymatic or 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) oxidized pretreatment were compared. The morphological properties, degree of polymerization, and crystallinity for the obtained nanofibrils, as well as physical and mechanical properties of the corresponding films were evaluated. Compared to refining, intense microfluidization contributed greater separation of nanofibril bundles, which led to an enhancement of mechanical strength and transparency for the resultant film. The selected enzymatic pre-treatments produced shortened fibers due to preferential hydrolysis of amorphous cellulose and, in combination with mechanical treatments, resulted in short and stiff cellulose nanocrystal (CNC)-like materials. Despite films from these CNC-like fibrils having inferior tensile strength, their tensile modulus and transparency were significantly improved compared to CNFs prepared without pre-treatment. The unique fiber morphology and high crystallinity potentially offer a green and ecologically friendly alternative for the preparation of CNCs and CNFs as part of an integrated biorefinery approach. Published by Elsevier Ltd.
期刊：
ACS Applied Materials and Interfaces,2015年7(8):5006-5016 ISSN：1944-8244
通讯作者：
Wu, QL
作者机构：
[Qing, Yan; Wu, Yiqiang] College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha, China;[Song, Kunlin; Wu, Qinglin; Li, Mei-Chun] School of Renewable Natural Resources, Louisiana State University Ag Center, Baton Rouge;[Song, Kunlin; Wu, Qinglin; Li, Mei-Chun] LA, United States;LA, United States
通讯机构：
[Wu, QL] Louisiana State Univ, AgCtr, Sch Renewable Nat Resources, Baton Rouge, LA 70803 USA.
关键词：
microfibrillated cellulose;cellulose nanocrystals;bentonite;water-based drilling fluids;rheology;filtration loss
作者：
Leslie A. Rescorla;Sofia Ginzburg;Thomas M. Achenbach;Masha Y. Ivanova;Fredrik Almqvist;Ivan Begovac;Niels Bilenberg;Hector Bird;Myriam Chahed;Anca Dobrean;Manfred Döpfner;Nese Erol;Helga Hannesdottir;Yasuko Kanbayashi;Michael C. Lambert;Patrick W. L. Leung;Asghar Minaei;Torunn S. Novik;Kyung-Ja Oh;Djaouida Petot
期刊：
JOURNAL OF CLINICAL CHILD AND ADOLESCENT PSYCHOLOGY,2013年42(02):262-273 ISSN：1537-4416
通讯作者：
Rescorla, LA
作者机构：
[Djaouida Petot; Jean-Michel Petot; Myriam Chahed] Paris Ouest Nanterre Def Univ, Dept Psychol, Paris, France.;[Jan van der Ende; Frank C. Verhulst] Erasmus Univ, Med Ctr, Sophia Childrens Hosp, Dept Child & Adolescent Psychiat, NL-3000 DR Rotterdam, Netherlands.;[Leslie A. Rescorla; Sofia Ginzburg] Bryn Mawr Coll, Dept Psychol, Bryn Mawr, PA 19010 USA.;[Tomasz Wolanczyk] Med Univ Warsaw, Dept Child Psychiat, Warsaw, Poland.;[Fredrik Almqvist; Sheila Weintraub] Univ Helsinki, Dept Child Psychiat, FIN-00014 Helsinki, Finland.
通讯机构：
[Rescorla, LA] Bryn Mawr Coll, Dept Psychol, 101 North Mer Ave, Bryn Mawr, PA 19010 USA.
摘要：
We used population sample data from 25 societies to answer the following questions: (a) How consistently across societies do adolescents report more problems than their parents report about them? (b) Do levels of parent–adolescent agreement vary among societies for different kinds of problems? (c) How well do parents and adolescents in different societies agree on problem item ratings? (d) How much do parent–adolescent dyads within each society vary in agreement on item ratings? (e) How well do parent–adolescent dyads within each society agree on the adolescent's deviance status? We used five methods to test cross-informant agreement for ratings obtained from 27,861 adolescents ages 11 to 18 and their parents. Youth Self-Report (YSR) mean scores were significantly higher than Child Behavior Checklist (CBCL) mean scores for all problem scales in almost all societies, but the magnitude of the YSR–CBCL discrepancy varied across societies. Cross-informant correlations for problem scale scores varied more across societies than across types of problems. Across societies, parents and adolescents tended to rate the same items as low, medium, or high, but within-dyad parent–adolescent item agreement varied widely in every society. In all societies, both parental noncorroboration of self-reported deviance and adolescent noncorroboration of parent-reported deviance were common. Results indicated many multicultural consistencies but also some important differences in parent–adolescent cross-informant agreement. Our findings provide valuable normative baselines against which to compare multicultural findings for clinical samples.
作者机构：
[Chengjun Zhou; Yiying Yue; Qinglin Wu; Qingfeng Shi] School of Renewable Natural Resource, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, United States;[Weihong Guo; Qingfeng Shi] School of Material Science and Engineering, East China University of Science and Technology, Shanghai 200237, China;[Yiqiang Wu] College of Material Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
通讯机构：
[Wu, QL] Louisiana State Univ, Ctr Agr, Sch Renewable Nat Resource, Baton Rouge, LA 70803 USA.
摘要：
Fibrous bio-nanocomposite mats consisting of cellulose nanocrystals (CNCs) and poly(lactic acid) (PIA) were electrospun from a solvent mixture consisting of N,N'-dimethylformamide and chloroform at room temperature. Morphological, mechanical and thermal properties, as well as in vitro degradation of nanocomposite mats were characterized as a function of material composition. Average diameter of the electrospun fibers decreased with increased CNC-loading level. Thermal stability, and tensile strength and modulus of nanocomposite mats were effectively improved by the addition of CNCs up to the 5 wt% level. The reinforcement of CNCs on electrospun mats was illustrated by the observation of SEM-based morphologies on the tensile fracturing process of nanocomposite mats. At the CNC content of 5 wt%, the maximum tensile stress and Young's modulus of the nanocomposite mats increased by 5 and 22 folds than those of neat PEA mats, respectively. Moreover, compared with neat PEA mats, the nanocomposite mats, especially at high CNC-loading levels, degraded more rapidly in phosphate-buffered saline solution. (C) 2012 Elsevier Ltd. All rights reserved.
作者机构：
[Kennedy, J. F.] Birmingham Carbohydrate and Protein Technology Group, School of Chemistry, University of Birmingham, Birmingham, B15 2TT, United Kingdom;[Sun, R. C.; Li, W. Y.; Liu, C. F.; Zhang, A. P.] State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510640, China;[Sun, R. C.] Institute of Biomass Chemistry and Technology, Beijing Forest University, Beijing, 100083, China;[Jin, A. X.] College of Natural Resources and Environmental Science, Central South Forestry University of Science and Technology, Changsha, China
通讯机构：
[Liu, CF] S China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Guangdong, Peoples R China.
作者机构：
[Hu, Xin-jiang] College of Environmental Science and Engineering Research, Central South University of Forestry and Technology, Changsha, China;[Zeng, Guang-ming; Xu, Yan; Hu, Xin-jiang; Liu, Si-mian; Liu, Yun-guo; Gu, Yan-ling; Li, Jiang; Tan, Xiao-fei] College of Environmental Science and Engineering, Hunan University, Changsha, China;[Zeng, Guang-ming; Xu, Yan; Hu, Xin-jiang; Liu, Si-mian; Liu, Yun-guo; Gu, Yan-ling; Li, Jiang; Tan, Xiao-fei] Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha, China;[Wang, Xin] College of Resources and Environmental Science, Hunan Normal University, Changsha, China;[Liu, Shao-bo] School of Metallurgy and Environment, Central South University, Changsha, China
通讯机构：
[Liu, Yun-Guo] Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address:
摘要：
Synthesizing biochar-based nano-composites can obtain new composites and combine the advantages of biochar with nano-materials. The resulting composites usually exhibit great improvement in functional groups, pore properties, surface active sites, catalytic degradation ability and easy to separation. These composites have excellent abilities to adsorb a range of contaminants from aqueous solutions. Particularly, catalytic material-coated biochar can exert simultaneous adsorption and catalytic degradation function for organic contaminants removal. Synthesizing biochar-based nano-composites has become an important practice for expanding the environmental applications of biochar and nanotechnology. This paper aims to review and summarize the various synthesis techniques for biochar-based nano-composites and their effects on the decontamination of wastewater. The characteristic and advantages of existing synthesis methods are summarized and discussed. Application of biochar-based nano-composites for different contaminants removal and the underlying mechanisms are reviewed. Furthermore, knowledge gaps that exist in the fabrication and application of biochar-based nano-composites are also identified. (C) 2016 Elsevier Ltd. All rights reserved.
期刊：
Remote Sensing of Environment,2014年140:279-293 ISSN：0034-4257
通讯作者：
Yuan, W.(wenpingyuancn@yahoo.com)
作者机构：
[ Xue, Yueju ] College of Information, South China Agricultural University, Guangzhou 510642, China;[ Feng, Jinming ; Wang, Kaicun ; Yuan, Wenping ; Xia, Jiangzhou ; Chen, Yang ] State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Global Change and Earth System Science, Beijing Normal University, Beijing 100875, China;[ Zha, Tonggang ] School of Resources and Environment, Beijing Forestry University, Beijing 100083, China;[ Zhao, Liang ] Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Institute of Plateau Biology, The Chinese Academy of Sciences, Xining, Qinghai 810008, China;[ Sun, Liang ] Department of Geographical Sciences, University of Maryland, College Park, MD 20742, United States
通讯机构：
[Yuan, WP] Beijing Normal Univ, Coll Global Change & Earth Syst Sci, State Key Lab Earth Surface Proc & Resource Ecol, Beijing 100875, Peoples R China.
摘要：
Evapotranspiration (ET) is a key component of terrestrial ecosystems because it links the hydrological, energy, and carbon cycles. Several satellite-based ET models have been developed for extrapolating local observations to regional and global scales, but recent studies have shown large model uncertainties in ET simulations. In this study, we compared eight ET models, including five empirical and three process-based models, with the objective of providing a reference for choosing and improving methods. The results showed that the eight models explained between 61 and 80% of the variability in ET at 23 eddy covariance towers in China and adjacent regions. The mean annual ET for all of China varied from 535 to 852 mm yr(-1) among the models. The interannual variability of yearly ET varied significantly between models during 1982-2009 because of different model structures and the dominant environmental factors employed. Our evaluation results showed that the parameters of the empirical methods may have different combination because the environmental factors of ET are not independent. Although the three process-based models showed high model performance across the validation Sites, there were substantial differences among them in the temporal and spatial patterns of ET, the dominant environment factors and the energy partitioning schemes. The disagreement among current ET models highlights the need for further improvements and validation, which can be achieved by investigating model structures and examining the ET component estimates and the critical model parameters. (C) 2013 Elsevier Inc. All rights reserved.
关键词：
Soil inorganic carbon;Carbon content;Carbon pool;Agricultural activity;Soil carbon loss
摘要：
The accurate estimation of soil carbon (C) stocks is a necessary component for understanding the global C budget. Although the importance of soil organic C (SOC) within the C cycle is well established, the quantity of soil inorganic C (SIC, including lithogenic and pedogenic inorganic C) pool, another important soil C pool component, has been poorly studied to date. In this study, soil profile data compiled by China's second national soil survey conducted in the 1980s was used to investigate the spatial distribution of SIC for the entire country under present day conditions as well as changes in SIC under historical land use. Results showed that the total SIC storage in China was approximately 55.3 +/- 10.7 Pg C with a current average content of 6.3 +/- 1.2 kg C m(-2), representing 5.8% of the global SIC pool. Land use has significantly affected SIC levels in cultivated soils. Approximately 51% of total cultivated soil surfaces in China have experienced C loss where the most significant loss has been observed in the eastern part of northern China in dry farmlands as well as irrigated soils and paddy soils. On the contrary, SIC has increased (similar to 10%) in irrigated soils in northwestern China. No significant change (similar to 39%) has been observed in soils in southern and the eastern part of northeast China. The total loss of SIC in China was approximately 1.6 Pg C due to extensive human activity. Results of this study indicate that human activity may have had a great impact on SIC as well as SOC pools. (C) 2008 Published by Elsevier B.V.
期刊：
JOURNAL OF FOOD ENGINEERING,2010年101(1):92-97 ISSN：0260-8774
通讯作者：
Shan, Y.(shanyang_jgs@163.com)
作者机构：
[ Su, Donglin ; Liu, Feng ; Shan, Yang ; Zhu, Xiangrong ; Li, Gaoyang ] Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China;[ Li, Shuifang ] College of Science, Central South University of Forestry and Technology, Changsha 410004, China;[ Zhang, Zhuoyong ] Department of Chemistry, Capital Normal University, Beijing 100048, China
关键词：
Triticum aestivum;ERF protein TaERF3;TaERF3 overexpression;salt and drought tolerance;stress-related genes;TaERF3 silencing
摘要：
Salinity and drought are major limiting factors of wheat (Triticum aestivum) productivity worldwide. Here, we report the function of a wheat ERF transcription factor TaERF3 in salt and drought responses and the underlying mechanism of TaERF3 function. Upon treatment with 250 mM NaCl or 20% polyethylene glycol (PEG), transcript levels of TaERF3 were rapidly induced in wheat. Using wheat cultivar Yangmai 12 as the transformation recipient, four TaERF3-overexpressing transgenic lines were generated and functionally characterized. The seedlings of the TaERF3-overexpressing transgenic lines exhibited significantly enhanced tolerance to both salt and drought stresses as compared to untransformed wheat. In the leaves of TaERF3-overexpressing lines, accumulation levels of both proline and chlorophyll were significantly increased, whereas H(2)O(2) content and stomatal conductance were significantly reduced. Conversely, TaERF3-silencing wheat plants that were generated through virus-induced gene silencing method displayed more sensitivity to salt and drought stresses compared with the control plants. Real-time quantitative RT-PCR analyses showed that transcript levels of ten stress-related genes were increased in TaERF3-overexpressing lines, but compromised in TaERF3-silencing wheat plants. Electrophoretic mobility shift assays showed that the TaERF3 protein could interact with the GCC-box cis-element present in the promoters of seven TaERF3-activated stress-related genes. These results indicate that TaERF3 positively regulates wheat adaptation responses to salt and drought stresses through the activation of stress-related genes and that TaERF3 is an attractive engineering target in applied efforts to improve abiotic stress tolerances in wheat and other cereals.
