摘要：
The detection techniques for glyphosate residues have garnered attention due to the potential toxicity associated. Herein, we introduced a fluorescent “turn-on” strategy, leveraging competitive coordination with iron, for glyphosate detection. We synthesized iron-based metal-organic frameworks (Fe-MOFs) at room temperature. Among them, 2,5-dihydroxyterephthalic acid (DOBDC) acted as a ligand to produce strong fluorescence, and iron ions acted as a quencher to quench the fluorescence of DOBDC. Another ligand, 2-nitroterephthalic acid, contained nitro quenching of the fluorescence, giving it a lower background. Glyphosate competed with the ligands for ions, weakening the interaction between ions and the ligands. This disruption restored the fluorescence emission at 535 nm, enabling glyphosate detection. To facilitate field application, Fe-MOFs were encapsulated within agarose to create a functional hydrogel. Glyphosate penetrated the hydrogel and resulting in fluorescence “turn-on”. Remarkably, this method facilitated visual detection through smartphone photography coupled with RGB (red/green/blue) analysis, offering a user-friendly and portable solution.
The detection techniques for glyphosate residues have garnered attention due to the potential toxicity associated. Herein, we introduced a fluorescent “turn-on” strategy, leveraging competitive coordination with iron, for glyphosate detection. We synthesized iron-based metal-organic frameworks (Fe-MOFs) at room temperature. Among them, 2,5-dihydroxyterephthalic acid (DOBDC) acted as a ligand to produce strong fluorescence, and iron ions acted as a quencher to quench the fluorescence of DOBDC. Another ligand, 2-nitroterephthalic acid, contained nitro quenching of the fluorescence, giving it a lower background. Glyphosate competed with the ligands for ions, weakening the interaction between ions and the ligands. This disruption restored the fluorescence emission at 535 nm, enabling glyphosate detection. To facilitate field application, Fe-MOFs were encapsulated within agarose to create a functional hydrogel. Glyphosate penetrated the hydrogel and resulting in fluorescence “turn-on”. Remarkably, this method facilitated visual detection through smartphone photography coupled with RGB (red/green/blue) analysis, offering a user-friendly and portable solution.
摘要：
Proactive maintenance is widely recognized for enhancing equipment reliability and reducing downtime costs. However, its role in optimizing spare parts production and distribution decisions remains underexplored, thereby limiting efficient cross-domain resource utilization within the supply-demand system. This paper addresses this gap by studying a maintenance-driven multi-stage joint optimization problem (MMJOP), which integrates flexible spare parts production, multi-vehicle distribution, and imperfect maintenance. We propose an optimal imperfect maintenance strategy to link these cross-domain business activities precisely, and further develop a mathematical model aimed at minimizing energy consumption on the supply side and operational costs on the demand side. To solve the MMJOP, we design an enhanced non-dominated neighbor immune algorithm, featuring a customized initialization operator and a problem-specific local search operator. Additionally, a Q -learning mechanism is employed to automatically select the most appropriate key parameters in the proposed algorithm. Extensive experiments indicate that: (1) the proposed components greatly enhance QNNIA's search performance; and (2) the QNNIA outperforms four well-known comparison algorithms regarding computational optimality, convergence, distribution, and stability. More importantly, the proposed model yields significant economic value, i.e., saving operational costs by 49% with negligible impact on overall energy consumption, proving the necessity of cross-domain business cooperation and resource optimization in the high-end equipment industry.
Proactive maintenance is widely recognized for enhancing equipment reliability and reducing downtime costs. However, its role in optimizing spare parts production and distribution decisions remains underexplored, thereby limiting efficient cross-domain resource utilization within the supply-demand system. This paper addresses this gap by studying a maintenance-driven multi-stage joint optimization problem (MMJOP), which integrates flexible spare parts production, multi-vehicle distribution, and imperfect maintenance. We propose an optimal imperfect maintenance strategy to link these cross-domain business activities precisely, and further develop a mathematical model aimed at minimizing energy consumption on the supply side and operational costs on the demand side. To solve the MMJOP, we design an enhanced non-dominated neighbor immune algorithm, featuring a customized initialization operator and a problem-specific local search operator. Additionally, a Q -learning mechanism is employed to automatically select the most appropriate key parameters in the proposed algorithm. Extensive experiments indicate that: (1) the proposed components greatly enhance QNNIA's search performance; and (2) the QNNIA outperforms four well-known comparison algorithms regarding computational optimality, convergence, distribution, and stability. More importantly, the proposed model yields significant economic value, i.e., saving operational costs by 49% with negligible impact on overall energy consumption, proving the necessity of cross-domain business cooperation and resource optimization in the high-end equipment industry.
期刊：
Methods in Molecular Biology,2025年2916:77-84 ISSN：1064-3745
作者机构：
[Li, Jia] Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan, People's Republic of China;[Li, Meng] College of Life and Environmental Sciences, Central South University of Forestry and Technology, Changsha, Hunan, People's Republic of China. limeng0422@csuft.edu.cn
关键词：
A. catechu;Enzyme activity;Fruitlet Abscission;Microscopic observation
摘要：
Fruitlet abscission is a bottleneck of the Areca catechu industry. A series of physiological and biochemical changes, such as morphological variations, cell architecture reconstruction, and increasing or decreasing enzymatic activity, occur during fruitlet abscission. There are species specificities among various plants concerning methods applied in abscission study. In this chapter, we describe the main methods adopted in the study focused on A. catechu fruitlet abscission, including microscopic observation, biochemical analysis, and abscission statistics. These methods are used to explore the physiological process and regulatory mechanism of fruitlet abscission in this industrial species.
期刊：
Journal of Chromatography A,2025年1755:466034 ISSN：0021-9673
通讯作者：
Xu, Youzhi
作者机构：
[Zeng, Xinyi; Fan, Guangjun; Zhong, Haiyan; Zeng, Xueying; Xu, Youzhi; Wang, Chunqin] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China;[Zeng, Xinyi; Fan, Guangjun; Zhong, Haiyan; Zeng, Xueying; Wang, Chunqin] National Engineering Laboratory for Rice and By-product Deep Processing, Changsha, 410004, China;[Liu, Bifeng; Feng, Xiaojun] Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China;[Xu, Youzhi] National Engineering Laboratory for Rice and By-product Deep Processing, Changsha, 410004, China. Electronic address: t20121499@csuft.edu.cn
通讯机构：
[Youzhi Xu] C;College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China<&wdkj&>National Engineering Laboratory for Rice and By-product Deep Processing, Changsha, 410004, China
关键词：
Antioxidant;Micromixer;On-line analysis;Optimization and validation;Rapid mixing
摘要：
Background The integration of high-performance liquid chromatography (HPLC) with rapid post-column biochemical detection techniques has gained widespread application in the pinpoint of antioxidant components in complex natural matrices. However, the use of PEEK reaction coils in on-line antioxidant analysis often encounters issues with poor mixing of the reaction solution, leading to peak broadening and a decrease in peak capacity. This may adversely affect the sensitivity and precision of the analysis. Microfluidic technology provides a unique opportunity for rapid solution mixing. Despite many continuous flow-based micromixers being reported, developing an ultrafast micromixer remains challenging.
The integration of high-performance liquid chromatography (HPLC) with rapid post-column biochemical detection techniques has gained widespread application in the pinpoint of antioxidant components in complex natural matrices. However, the use of PEEK reaction coils in on-line antioxidant analysis often encounters issues with poor mixing of the reaction solution, leading to peak broadening and a decrease in peak capacity. This may adversely affect the sensitivity and precision of the analysis. Microfluidic technology provides a unique opportunity for rapid solution mixing. Despite many continuous flow-based micromixers being reported, developing an ultrafast micromixer remains challenging.
Results Here, we demonstrated a new microfluidic mixer and used it to create a highly sensitive on-line HPLC-ABTS system for antioxidant screening, replacing the PEEK reaction coil. The mixer utilizes the engulfment flow to achieve complete mixing of two solutions within sub-microseconds (∼0.6 µs). The system demonstrated consistent half-peak widths under 0.63 min for five phenolic compounds, with a 0.1 min delay time. Optimization of the HPLC-ABTS assay included determining the ideal ABTS •+ concentration and flow rate ratio of ABTS •+ solution to post-column mobile phase. Validation showed that the system exhibited low detection limits (0.3-1.4 μg/mL), superior linearity (r > 0.9900), acceptable recovery (92.33-109.33%), high stability (RSD < 4.96%), and excellent reproducibility (RSD < 2.3%). These observations highlight the advantages of employing the micromixer in on-line analysis.
Here, we demonstrated a new microfluidic mixer and used it to create a highly sensitive on-line HPLC-ABTS system for antioxidant screening, replacing the PEEK reaction coil. The mixer utilizes the engulfment flow to achieve complete mixing of two solutions within sub-microseconds (∼0.6 µs). The system demonstrated consistent half-peak widths under 0.63 min for five phenolic compounds, with a 0.1 min delay time. Optimization of the HPLC-ABTS assay included determining the ideal ABTS •+ concentration and flow rate ratio of ABTS •+ solution to post-column mobile phase. Validation showed that the system exhibited low detection limits (0.3-1.4 μg/mL), superior linearity (r > 0.9900), acceptable recovery (92.33-109.33%), high stability (RSD < 4.96%), and excellent reproducibility (RSD < 2.3%). These observations highlight the advantages of employing the micromixer in on-line analysis.
Significance Peak broadening reduces the selectivity and accuracy of on-line HPLC assays for detecting antioxidants in complex matrices. Compared to conventional on-line assays utilizing a PEEK coil for radical scavenging, our newly developed system demonstrates enhanced selectivity and precision. This on-line system can be combined with mass spectrometry or on-line extraction devices, which presents a promising alternative for the rapid screening of antioxidant compounds in plant products.
Peak broadening reduces the selectivity and accuracy of on-line HPLC assays for detecting antioxidants in complex matrices. Compared to conventional on-line assays utilizing a PEEK coil for radical scavenging, our newly developed system demonstrates enhanced selectivity and precision. This on-line system can be combined with mass spectrometry or on-line extraction devices, which presents a promising alternative for the rapid screening of antioxidant compounds in plant products.
作者机构：
[Lu, Mengqi; Xiao, Zhun; Lu, Kaizheng; Zeng, Yanling; Wang, Jinfeng; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zeng, YL] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.;[Xiao, Zhun] Changsha Social Work Coll, Sch Foreign Languages, Changsha 410004, Peoples R China.;[Zeng, Yanling; Tan, Xiaofeng; Zeng, YL] Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.;[Lu, Mengqi; Xiao, Zhun; Lu, Kaizheng; Zeng, Yanling; Wang, Jinfeng; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zeng, YL] Cent South Univ Forestry & Technol, Camellia Oil Tree Res Acad, Changsha 410004, Peoples R China.;[Lu, Mengqi] Hunan Acad Forestry, Changsha 410004, Peoples R China.
通讯机构：
[Tan, XF ; Zeng, YL] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Camellia Oil Tree Res Acad, Changsha 410004, Peoples R China.
关键词：
Camellia Oleifera;Camellia lanceoleosa;Mitochondrial genome;De novo assembly;Phylogenetic analysis
摘要：
Camellia-oil trees are economically valuable, oil-rich species within the genus Camellia, family Theaceae. Among these species, C. oleifera, a member of Section Oleifera in the genus, is the most extensively cultivated in China. In this study, we assembled the mitochondrial genomes (mitogenomes) of two Camellia species, namely C. oleifera and C. lanceoleosa. These two species are closely related and belong to the same genus and section, with C. oleifera being hexaploid and C. lanceoleosa being diploid. The mitogenome of C. oleifera is comprised of 1,039,838 base pairs (bp), and C. lanceoleosa is comprised of 934,155bp. Both genomes exhibit a multipartite genome structure, which is supported by our PCR experiments. We conducted codon usage and RNA editing site analysis on these two mitogenomes, which showed highly consistent results. However, analysis of repetitive sequences and mitochondrial plastid sequences (MTPTs) revealed differences between the two mitogenomes. Phylogenetic analysis indicated that these two species clustered together, suggesting a close evolutionary relationship. The collinearity analysis results showed extensive genome rearrangements in the mitogenomes of Camellia species. We successfully assembled the mitogenomes of C. oleifera and C. lanceoleosa, marking a significant advancement in understanding camellia-oil tree mitogenomes. Unlike circular mitogenomes reported before, our research confirms multiple-branched configurations in these two species. This sheds light on mitogenome structural complexities and contributes to our understanding of evolutionary processes. Additionally, these results enrich Camellia genetic resources and expand our knowledge of mitogenome variation.
通讯机构：
[Huang, SH ] C;[He, ZX ] M;Cent South Univ Forestry & Technol, Coll Life & Environm Sci, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Yuelushan Lab, Changsha 410004, Peoples R China.;Minist Ecol & Environm, Tech Ctr Soil Agr & Rural Ecol & Environm, Beijing 100012, Peoples R China.
关键词：
Ni contaminatied soil;Stabilization;Fe-Mn modified biochar
摘要：
PurposeDue to the persistence and limited degradation capacity of Ni, Ni concentrations in soil are gradually increasing, posing a great challenge for soil remediation. The objective of this study was to remediate Ni contaminated soil using ferromanganese modified biochar (FMBC) and to elucidate its stabilization mechanism.Materials and methodsIn this study, using SEM-EDS, XRD, FTIR, XPS as well as adsorption thermodynamic and kinetic models, study the binding characteristics of the material and Ni and the stabilization mechanism of the material on Ni -contaminated soil. Toxicity Characteristic Leaching Profile (TCLP) and BCR sequential extraction procedures were used to study the stabilizing effect of this material on Ni in soil in soil incubation tests.Results and discussionThe experimental results show that multilayer chemisorption is the dominant adsorption mechanism, and the stabilization process is mainly dominated by hydrogen bonding, ion exchange of Fe/Mg-O and Ni(II), conjugation effects of aromatic groups containing pi-bonds, ketone, carboxyl or ester groups, and surface complexation. TCLP leaching Ni concentrations in contaminated soil can be reduced by over 98% after 30 days of stable recovery at 1% material dosage. Besides, weak acid extractable and reducible Ni concentrations were reduced by 33% and 24%, respectively. Meanwhile, oxidizable and residual Ni concentrations increased by 44% and 14%, respectively.ConclusionsThese results suggest that Ni in soils has changed from a highly mobile and bioavailable state to a more stable and less ecotoxic state. Therefore, FMBC is a highly promising material for the remediation of Ni-contaminated soils.
摘要：
INTRODUCTION: Orobanche coerulescens is a parasitic plant considered as a malignant weed due to its harmful effects on crops. However, its richness in high-value secondary metabolites makes it a significant medicinal resource. The development of microspores and megaspores is essential for sexual reproduction in plants but research on this aspect of O. coerulescens is lacking. METHODS: This study aimed to systematically observe the developmental processes of microspores and megaspores in O. coerulescens using microscopic techniques. We measured the levels of soluble sugar, starch, and phytohormones during different developmental stages. We also investigated the key regulatory genes in the metabolic pathways of phytohormones that are closely related to the development of microspores and megaspores using transcriptome sequencing technology. RESULTS AND DISCUSSION: The findings revealed that the flower development process of O. coerulescens could be categorized into six stages. Mature pollen was tricellular, with downy ornamentation and pores on the outer wall. The embryo sac was the monosporangiate polygonum type, and the ovule was inverted. The megaspores developed and matured about 15 days later than the microspores. The soluble sugar level of the flower buds decreased initially and then increased during development, whereas the starch level showed an opposite trend. The levels of strigolactone, auxin, and gibberellins gradually increased throughout the development process. The key genes regulating phytohormone synthesis during the development of microspores and megaspores were identified as ALDHs (Aldehyde Dehydrogenases). In contrast, the key genes regulating phytohormone signaling included TIR1 (Transport Inhibitor Response 1) and IAA3 (Indole-3-acetic Acid Inducible 3), and the key TF was ARF5 (AUXIN RESPONSE FACTOR 5). The findings of this study enhanced the understanding of O. coerulescens biology, providing theoretical references for regulating its reproduction, implementing biological control measures, maintaining its population, and optimizing resource utilization.
通讯机构：
[He, JN ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.
关键词：
M. Alternifolia;Provide shade;Photosynthesis;Chlorophyll;Endogenous hormone;Paraffin section
摘要：
In light of the detrimental effects of global warming, exacerbated by the intense sunlight and high temperatures prevalent during summer in southern China, on the growth of Melaleuca alternifolia (M. alternifolia) seedlings, this study sets out to examine the impact of various shading treatments on the growth and physiological attributes of M. alternifolia seedlings, with the aim of determining the optimum shading level. The seedling phase is of paramount importance in the growth cycle and regeneration process of M. alternifolia, as both excessive and insufficient light exposure can negatively influence seedling development. Four shading regimes (CK: no shading; C1: 35-45% shading; C2: 45-50% shading; C3: 60-70% shading) were imposed on M. alternifolia seedlings, following which observations were conducted after a 35-day period. The results reveal that the different shading treatments had a significant effect on the growth and physiological indicators of the seedlings, with the moderate shading treatment (C2) proving to be the most advantageous for seedling growth. Under the C2 treatment, an increase in chlorophyll content was observed in the leaves of the seedlings, accompanied by a decrease in malondialdehyde (MDA) content. Concurrently, there was a reduction in the activities of antioxidant enzymes, namely superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Furthermore, the levels of abscisic acid (ABA) and gibberellic acid (GA3) declined, while the concentrations of auxin (IAA), salicylic acid (SA), and cytokinin (CTK) ascended. Leaf anatomical analysis disclosed that, in comparison to the control group, the thickness of the lower epidermis and palisade tissue in the leaves of the seedlings subjected to the C2 treatment exhibited a significant reduction. Additionally, an increase in the number of spongy tissue cells was noted, along with a marked increase in total leaf thickness. Consequently, cultivating M. alternifolia seedlings under shading conditions ranging from 45 to 50% is deemed more favorable. The findings of this study offer novel insights into the adaptive responses of M. alternifolia seedlings to varying light intensities and provide theoretical guidance for the cultivation and management of M. alternifolia seedlings in the southern regions of China.
摘要：
As an excellent variety of Camphora glanduliferum, Honganzhang has high essential oil content and can be used as industrial and pharmaceutical raw materials to extract essential oil, which has high development value. In this study, fresh branches and leaves of C. glanduliferum 'Honganzhang' were used as materials. After extracting C. glanduliferum 'Honganzhang' essential oils (CGHEO) by steam distillation, gas chromatography-mass spectrometry (GC-MS) was used to analyze its chemical components. On this basis, the antibacterial effect of CGHEO and its potential physiological mechanism were further explored. The results showed that the main components of CGHEO were Cineole (46.57%), alpha-terpineol (9.93%), Sabinene (8.02%), beta-terpinene (6.04%), and alpha-pinene (4.49%), these compounds can be used in medicine, food and other industries. In terms of antibacterial effect, CGHEO has significant antibacterial effects on three Gram-negative bacteria (Escherichia coli, Salmonella enteritidis, and Pseudomonas aeruginosa), and two Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus). CGHEO can reduce Na+K+-ATPase activity and SDH activity and further interfere with the operation of the bacterial respiratory chain, thereby achieving the effect of inhibiting bacterial reproduction. Based on the main chemical components contained in C. glanduliferum 'Honganzhang' and its remarkable antibacterial effect, this study is expected to provide a theoretical basis for the wide application of C. glanduliferum 'Honganzhang' in multiple fields such as medicine, health care, food, and industry.
摘要：
This paper proposes beam-only connected steel plate shear walls (BSWs) and composite shear walls (BCWs) to mitigate the seismic risk of concrete-filled double-steel tube (CFDST) frames. As a new type of structural system, limited works have been reported on the performance limit definitions and risk-informed assessment of them due to the lack of experimental and analytical data. According to the cyclic tests results performed by the authors and the guidance of FEMA 356, three stage of limit states are defined and the corresponding results are recommended. Then, an elaborate numerical model is developed that considering the nonlinear behaviour of materials, panel zone, beam-column joints and shear walls, and the model is validated by the test results. Besides, three CFDST buildings with different height and two CFDST buildings with BSW or BCW are modeled based on the validated numerical model. Finally, seismic fragility analyses as well as seismic risk assessments are conducted on these models, and the annual occurrence probability and collapse probability of the structure within 50 years are calculated. The results show that both BSWs and BCWs can effectively mitigate the seismic responses and risks of CFDST buildings, and the collapse probability of buildings within 50 years decreases from 7.7 % to 1.39 % and 1.24 % due to the equipped BSWs and BCWs, respectively.
This paper proposes beam-only connected steel plate shear walls (BSWs) and composite shear walls (BCWs) to mitigate the seismic risk of concrete-filled double-steel tube (CFDST) frames. As a new type of structural system, limited works have been reported on the performance limit definitions and risk-informed assessment of them due to the lack of experimental and analytical data. According to the cyclic tests results performed by the authors and the guidance of FEMA 356, three stage of limit states are defined and the corresponding results are recommended. Then, an elaborate numerical model is developed that considering the nonlinear behaviour of materials, panel zone, beam-column joints and shear walls, and the model is validated by the test results. Besides, three CFDST buildings with different height and two CFDST buildings with BSW or BCW are modeled based on the validated numerical model. Finally, seismic fragility analyses as well as seismic risk assessments are conducted on these models, and the annual occurrence probability and collapse probability of the structure within 50 years are calculated. The results show that both BSWs and BCWs can effectively mitigate the seismic responses and risks of CFDST buildings, and the collapse probability of buildings within 50 years decreases from 7.7 % to 1.39 % and 1.24 % due to the equipped BSWs and BCWs, respectively.
作者：
Liangjun Zhu;Danyang Yuan;J. Julio Camarero;David J. Cooper;Mai-He Li;...
期刊：
森林生态系统(英文),2025年:100336 ISSN：2095-6355
通讯作者：
Xiaochun Wang
作者机构：
School of Ecology, Northeast Forestry University, Harbin 150040, China;Swiss Federal Institute for Forest, Snow and Landscape Research WSL, Birmensdorf, Switzerland;College of Life and Environmental Science, Central South University of Forestry and Technology, Changsha 410004, China;[J. Julio Camarero] Instituto Pirenaico de Ecología (IPE-CSIC), Avda. Montañana 1005, E-50192 Zaragoza, Spain;[David J. Cooper] Department of Forest and Rangeland Stewardship and Graduate Degree Program in Ecology, Colorado State University, Fort Collins, CO 80523, USA
通讯机构：
[Xiaochun Wang] S;School of Ecology, Northeast Forestry University, Harbin 150040, China<&wdkj&>School of Life, Qufu Normal University, Qufu, China
关键词：
Tree rings;Growth synchrony;Forest resilience;Rapid warming;Temperate Forest;Northeast China
摘要：
Tree growth synchrony serves as a valuable ecological indicator of forest resilience to climate stress and disturbances. However, our understanding of how increasing temperature affects tree growth synchrony during rapidly and slowly warming periods in ecosystems with varying climatic conditions remains limited. By using tree-ring data from temperate broadleaf ( Fraxinus mandshurica , Phellodendron amurense , Quercus mongolica , and Juglans mandshurica ) and Korean pine ( Pinus koraiensis ) mixed forests in northeast China, we investigated the effects of climate change, particularly warming, on the growth synchrony of five dominant temperate tree species across contrasting warm-dry and cool-wet climate conditions. Results show that temperature over water availability was the primary factor driving the growth and growth synchrony of the five species. Growth synchrony was significantly higher in warm-dry than in cool-wet areas, primarily due to more uniform climate conditions and higher climate sensitivity in the former. Rapid warming from the 1960s to the 1990s significantly enhanced tree growth synchrony in both areas, followed by a marked reversal as temperatures exceeded a certain threshold or warming slowed down, particularly in the warm-dry area. The growth synchrony variation patterns of the five species were highly consistent over time, although broadleaves exhibited higher synchrony than conifers, suggesting potential risks to forest resilience and stability under future climate change scenarios. Growing season temperatures and non-growing season temperatures and precipitation had a stronger positive effect on tree growth in the cool-wet area compared to the warm-dry area. High relative humidity hindered growth in the cool-wet area but enhanced it in the warm-dry area. Overall, our study highlights that the diversity and sensitivity of climate-growth relationships directly determine spatiotemporal growth synchrony. Temperature, along with water availability, shape long-term forest dynamics by affecting tree growth and synchrony. These results provide crucial insights for forest management practice to enhance structural diversity and resilience capacity against climate change-induced synchrony shifts.
Tree growth synchrony serves as a valuable ecological indicator of forest resilience to climate stress and disturbances. However, our understanding of how increasing temperature affects tree growth synchrony during rapidly and slowly warming periods in ecosystems with varying climatic conditions remains limited. By using tree-ring data from temperate broadleaf ( Fraxinus mandshurica , Phellodendron amurense , Quercus mongolica , and Juglans mandshurica ) and Korean pine ( Pinus koraiensis ) mixed forests in northeast China, we investigated the effects of climate change, particularly warming, on the growth synchrony of five dominant temperate tree species across contrasting warm-dry and cool-wet climate conditions. Results show that temperature over water availability was the primary factor driving the growth and growth synchrony of the five species. Growth synchrony was significantly higher in warm-dry than in cool-wet areas, primarily due to more uniform climate conditions and higher climate sensitivity in the former. Rapid warming from the 1960s to the 1990s significantly enhanced tree growth synchrony in both areas, followed by a marked reversal as temperatures exceeded a certain threshold or warming slowed down, particularly in the warm-dry area. The growth synchrony variation patterns of the five species were highly consistent over time, although broadleaves exhibited higher synchrony than conifers, suggesting potential risks to forest resilience and stability under future climate change scenarios. Growing season temperatures and non-growing season temperatures and precipitation had a stronger positive effect on tree growth in the cool-wet area compared to the warm-dry area. High relative humidity hindered growth in the cool-wet area but enhanced it in the warm-dry area. Overall, our study highlights that the diversity and sensitivity of climate-growth relationships directly determine spatiotemporal growth synchrony. Temperature, along with water availability, shape long-term forest dynamics by affecting tree growth and synchrony. These results provide crucial insights for forest management practice to enhance structural diversity and resilience capacity against climate change-induced synchrony shifts.
通讯机构：
[Wang, HD ] C;Cent South Univ Forestry & Technol, Sch Civil Engn, Changsha 410004, Peoples R China.
关键词：
Calcareous sand;EICP;Stress-strain curve;Calcium carbonate content;Statistical damage constitutive model
摘要：
The enzyme-induced calcium carbonate precipitation (EICP) technique was utilized to cement calcareous sand. The mechanical properties of EICP-cemented calcareous sand at various cementation degree were investigated using consolidated drained triaxial compression tests. A statistical damage constitutive model tailored for EICP-cemented calcareous sand was also developed based on damage mechanics theory. The findings are as follows: (1) The EICP technique significantly enhances the cementation of calcareous sand. As the number of grouting operations increases, the peak deviator stress of the cemented material gradually increases, with the maximum enhancement approaching 2.5 times. Moreover, during the stress decay phase following the peak stress, the decay rate of the cemented sand accelerates, displaying a more pronounced brittle characteristic. (2) With the increased calcium carbonate content, the peak eccentric stress of the cemented body increases significantly, and there is an obvious nonlinear exponential correlation between them. (3) The statistical damage constitutive model, formulated based on Lemaitre's strain equivalence principle combined with a log-normal distribution and the Drucker–Prager strength criterion, accurately predicts the stress–strain curves, effectively simulating the complete stress–strain evolution of EICP-cemented sand under different numbers of grouting operations and varied confining pressure conditions. (4) At higher cementation levels or lower confining pressures, the internal damage process of the EICP-cemented calcareous sand specimens intensifies, indicated by the rapid increase of the damage variable D with axial strain. The research findings can provide a crucial theoretical foundation for the application of EICP technology in the treatment of island reef or roadbed foundations, aiding in the analysis and prediction of the mechanical properties of EICP-cemented calcareous sands.
摘要：
Afforestation is gaining global attention for its role in carbon sequestration and timber production. Afforestation affects hydrological processes in forest ecosystems, including water yield. However, there is a paucity of long-term observation data to examine the effects of afforestation on water yield. Moreover, drought caused by global warming could change the runoff generation process and make the forest–water relationship hotly debated. To fully assess these effects, we used 22-year data from a long-time observation from 1997 to 2019 (excluding missing data in 2017) in a Chinese fir ( Cunninghamia lanceolata (Lamb) Hook.) plantation in subtropical China. We analyzed the response of runoff to drought and stand growth post afforestation. Average runoff during drought declined by 41.2%, 37.5%, and 3.7% lower than nondrought events at monthly, seasonal, and yearly timescales, respectively. The drought affected runoff significantly when a drought event lasted 1.2 months, and drought severity was larger than 1.5. Annual runoff and runoff anomaly decreased with stand age during the first 14 years after afforestation and thereafter started to recover. A similar change pattern was found for the relationship between annual runoff and stand biomass. Annual runoff also decreased with an increase in the basal area. Monthly runoff was primarily controlled by rainfall and soil water rather than stand characteristics, while annual runoff largely depended on stand biomass. These findings clarify the relationship between planted forests and water resources and provide insightful information for sustainable water management in forests under global climate change.
Afforestation is gaining global attention for its role in carbon sequestration and timber production. Afforestation affects hydrological processes in forest ecosystems, including water yield. However, there is a paucity of long-term observation data to examine the effects of afforestation on water yield. Moreover, drought caused by global warming could change the runoff generation process and make the forest–water relationship hotly debated. To fully assess these effects, we used 22-year data from a long-time observation from 1997 to 2019 (excluding missing data in 2017) in a Chinese fir ( Cunninghamia lanceolata (Lamb) Hook.) plantation in subtropical China. We analyzed the response of runoff to drought and stand growth post afforestation. Average runoff during drought declined by 41.2%, 37.5%, and 3.7% lower than nondrought events at monthly, seasonal, and yearly timescales, respectively. The drought affected runoff significantly when a drought event lasted 1.2 months, and drought severity was larger than 1.5. Annual runoff and runoff anomaly decreased with stand age during the first 14 years after afforestation and thereafter started to recover. A similar change pattern was found for the relationship between annual runoff and stand biomass. Annual runoff also decreased with an increase in the basal area. Monthly runoff was primarily controlled by rainfall and soil water rather than stand characteristics, while annual runoff largely depended on stand biomass. These findings clarify the relationship between planted forests and water resources and provide insightful information for sustainable water management in forests under global climate change.
摘要：
Enhancing the stability and durability of superhydrophobic wood remains a significant challenge for its long-term application in various fields. This study presents a novel approach to developing durable superhydrophobic wood by regulating wood structure. The analyses of the mechanism revealed that Si-Ti@PDMS prepolymer infiltrated wood’s pores and cell walls, forming a highly cross-linked micro-nanoscale superhydrophobic coating extending from the exterior to the interior. The resulting superhydrophobic wood exhibited excellent hydrophobic characteristics on both its surface and various cutting surfaces. Furthermore, the water contact angles (WCA) measured on the various cut surfaces of the wood consistently exceeded 150°, thereby confirming its superhydrophobicity. Additionally, the water contact angles (WCA) at wood depth surfaces remained above 130°. This observation indicates that the non-wettability characteristic of the superhydrophobic wood extends from the surface to the interior. Consequently, even in the event of surface structural damage, the wood retains its robust hydrophobic performance. This study provides a theoretical foundation for regulating durable superhydrophobic wood, and it was beneficial to the efficient use of superhydrophobic wood in construction and furniture fields.
Enhancing the stability and durability of superhydrophobic wood remains a significant challenge for its long-term application in various fields. This study presents a novel approach to developing durable superhydrophobic wood by regulating wood structure. The analyses of the mechanism revealed that Si-Ti@PDMS prepolymer infiltrated wood’s pores and cell walls, forming a highly cross-linked micro-nanoscale superhydrophobic coating extending from the exterior to the interior. The resulting superhydrophobic wood exhibited excellent hydrophobic characteristics on both its surface and various cutting surfaces. Furthermore, the water contact angles (WCA) measured on the various cut surfaces of the wood consistently exceeded 150°, thereby confirming its superhydrophobicity. Additionally, the water contact angles (WCA) at wood depth surfaces remained above 130°. This observation indicates that the non-wettability characteristic of the superhydrophobic wood extends from the surface to the interior. Consequently, even in the event of surface structural damage, the wood retains its robust hydrophobic performance. This study provides a theoretical foundation for regulating durable superhydrophobic wood, and it was beneficial to the efficient use of superhydrophobic wood in construction and furniture fields.
摘要：
Chloroplasts, essential to the photosynthetic process, are pivotal in orchestrating the development and growth of plants. The pyruvate dehydrogenase complex (PDC) serves as a link between the citric acid cycle and glycolysis, supplying energy and carbon skeletons vital for plant metabolism. However, the involvement of the PDC in chloroplast development is not yet fully elucidated. Here, we provide evidence for the role of the PDC E1 component β subunit (PDC-E1β) in tobacco ( Nicotiana tabacum L.) chloroplast development and amino acid metabolism. Transgenic tobacco plants with RNA interference (RNAi) targeting four PDC-E1β genes displayed a pale-green variegated leaf phenotype. The phenotype exhibited decreased chlorophyll and defective chloroplast development. Transcriptome and metabolome analyses revealed significant alterations in genes and metabolites related to photosynthesis, amino acid metabolism, and chlorophyll biosynthesis. Silencing of PDC-E1β genes downregulated PEP-dependent chloroplast gene expression and chloroplast protein levels. Moreover, silencing of PDC-E1β genes disrupted amino acid metabolism, with the variegated phenotype partially reversible by exogenous tryptophan supplementation. Our findings elucidate the significance of PDC-E1β in chloroplast development and the interconnected metabolic pathways that regulate plant organelle function. This research provides novel perspectives on the intricate mechanisms governing chloroplast development and the metabolic pathways that sustain it.
Chloroplasts, essential to the photosynthetic process, are pivotal in orchestrating the development and growth of plants. The pyruvate dehydrogenase complex (PDC) serves as a link between the citric acid cycle and glycolysis, supplying energy and carbon skeletons vital for plant metabolism. However, the involvement of the PDC in chloroplast development is not yet fully elucidated. Here, we provide evidence for the role of the PDC E1 component β subunit (PDC-E1β) in tobacco ( Nicotiana tabacum L.) chloroplast development and amino acid metabolism. Transgenic tobacco plants with RNA interference (RNAi) targeting four PDC-E1β genes displayed a pale-green variegated leaf phenotype. The phenotype exhibited decreased chlorophyll and defective chloroplast development. Transcriptome and metabolome analyses revealed significant alterations in genes and metabolites related to photosynthesis, amino acid metabolism, and chlorophyll biosynthesis. Silencing of PDC-E1β genes downregulated PEP-dependent chloroplast gene expression and chloroplast protein levels. Moreover, silencing of PDC-E1β genes disrupted amino acid metabolism, with the variegated phenotype partially reversible by exogenous tryptophan supplementation. Our findings elucidate the significance of PDC-E1β in chloroplast development and the interconnected metabolic pathways that regulate plant organelle function. This research provides novel perspectives on the intricate mechanisms governing chloroplast development and the metabolic pathways that sustain it.
作者机构：
[Ding, Yuqin; Ding, YQ; Zhang, Lingzhi; Tang, Wanting; Lin, Lizhong; Ren, Jing; Mo, Yijie; Lin, Yanxin; Guo, Xiao] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Res Ctr Rice & By Prod Deep Proc, Changsha 410004, Peoples R China.
通讯机构：
[Ding, YQ ] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Res Ctr Rice & By Prod Deep Proc, Changsha 410004, Peoples R China.
关键词：
Mannan oligosaccharides;Curdlan;Cryoprotection activity;Surimi;Myofibrillar protein structure;Freeze-thaw cycles
摘要：
The cryoprotective effects of mannan oligosaccharides (MOS) and curdlan (CU) on the quality of grass carp surimi after freeze-thaw cycles (FTCs) were assessed using the response surface methodology. The optimal contents of MOS (6.79 %, w /w) and CU (0.45 %, w/w) produced minimum thawing losses and the highest gel strength of surimi after five times FTCs. MOS, CU, and their mixture demonstrated cryoprotective effects on grass carp surimi. Compared to MOS or CU alone, MOS-CU displayed synergistic cryoprotective effects, as evidenced by the better prevention of thawing losses of surimi, the superior retardation of the aggregation and denaturation of MP, the amelioration of the gel strength and WHC of surimi gel. Moreover, the MOS-CU mixture demonstrated cryoprotective effects equivalent to those of commercial cryoprotectant on grass carp surimi from zero to five times FTCs and even outperformed CC after seven times FTCs.
The cryoprotective effects of mannan oligosaccharides (MOS) and curdlan (CU) on the quality of grass carp surimi after freeze-thaw cycles (FTCs) were assessed using the response surface methodology. The optimal contents of MOS (6.79 %, w /w) and CU (0.45 %, w/w) produced minimum thawing losses and the highest gel strength of surimi after five times FTCs. MOS, CU, and their mixture demonstrated cryoprotective effects on grass carp surimi. Compared to MOS or CU alone, MOS-CU displayed synergistic cryoprotective effects, as evidenced by the better prevention of thawing losses of surimi, the superior retardation of the aggregation and denaturation of MP, the amelioration of the gel strength and WHC of surimi gel. Moreover, the MOS-CU mixture demonstrated cryoprotective effects equivalent to those of commercial cryoprotectant on grass carp surimi from zero to five times FTCs and even outperformed CC after seven times FTCs.
摘要：
Microcystin-LR (MC-LR) is a toxin that causes hepatic steatosis. Our previous study found that exposure to 60 μg/L MC-LR for 9 months resulted in liver lipid accumulation, but the underlying mechanisms remain elusive. Herein, for the first time, fatty acid-targeted metabolome and RNA-seq were combined to probe the effect and mechanism of chronic (12-month) MC-LR treatment on mice lipid metabolism at environmental-related levels (1, 60, and 120 μg/L). It was found that MC-LR dose-dependently raised serum and liver lipid levels. The total cholesterol (TC) levels in the liver were significantly increased following treatment with 1 μg/L MC-LR (equivalent to 0.004 μ/L in human). Treatment with 60 and 120 μg/L MC-LR significantly elevated TC and triglyceride (TG) levels in both serum and liver. Serum fatty acid-targeted metabolome analysis demonstrated that exposure to 1, 60, and 120 μg/L MC-LR caused significant alterations in the fatty acid profile. Chronic 1, 60, and 120 μg/L MC-LR treatment significantly increased serum polyunsaturated fatty acids (PUFAs), including conjugated linoleic acid and eicosapentaenoic acid, which positively correlated with serum or liver TG levels. Chronic exposure to 120 μg/L MC-LR led to a significant decrease in the accumulation of saturated fatty acids, including citramalic acid, pentadecanoic acid, and docosanoic acid, which were negatively correlated with serum or liver lipid levels. These findings suggested that 1 μg/L MC-LR exposure caused mild lipid metabolism disruption, while 60 and 120 μg/L MC-LR treatment resulted in pronounced hepatic steatosis in mice. Transcriptome analysis revealed that chronic environmental MC-LR treatment regulated the expression of genes involved in the phosphatidylinositol 3-kinase (PI3K) complex and fatty acid metabolism. Western blotting and RT-qPCR confirmed that chronic environmental MC-LR exposure activated the PI3K/AKT/mTOR signaling pathway, the downstream of fads3 gene that participates in fatty acid desaturation was upregulated, fatty acid degradation-related genes, including acsl1, acsl4, and ehhadh were inhibited, and lipid transport-related genes, including slc27a4 and apol7a, were promoted. Thus, chronic environmental MC-LR exposure boosts hepatic steatosis. Our work indicated that the limit concentration of 1 μg/L MC-LR in human drinking water for safety needs to be discussed. The study provides the first evidence of the fatty acid profile and gene changes and gains new insights into the mechanisms of chronic environmental MC-LR treatment-induced hepatic steatosis.
期刊：
Economic Systems Research,2025年37(1):1-29 ISSN：0953-5314
通讯作者：
Xie, R
作者机构：
[Zhao, Guomei] Cent South Univ Forestry & Technol, Sch Econ, Changsha, Peoples R China.;[Zhao, Guomei] Res Ctr High Qual Dev Ind Econ, Key Res Base Philosophy & Social Sci Hunan Prov, Changsha, Peoples R China.;[Xie, Rui] Hunan Univ, Sch Econ & Trade, Changsha, Peoples R China.;[Su, Bin] Natl Univ Singapore, Energy Studies Inst, Singapore, Singapore.;[Wang, Qunwei] Nanjing Univ Aeronaut & Astronaut, Sch Econ & Management, Nanjing, Peoples R China.
通讯机构：
[Xie, R ] H;Hunan Univ, Changsha 410079, Peoples R China.
摘要：
This paper constructs a comparative analysis framework on how the input-output (IO) model with technical differences affects the calculation of the pollution terms of trade (PTT) and the tests of the pollution haven hypothesis. Specifically, the CO 2 terms of trade (CTT) of the world's major economies are calculated based on five IO models, and chain additive structure decomposition analysis (SDA) is conducted to examine the roles of different factors in the changes in CTT. The economic phenomena reflected by the CTT measured by these IO models are found to be different, and a comparative analysis shows that different IO models are suitable for studying different economic problems. Suggestions are provided on the application of different IO models in the calculation of economic indicators and the study of economic issues. Policy makers need to be cautious about policy recommendations based on the results obtained from different IO models.
期刊：
Colloids and Surfaces A: Physicochemical and Engineering Aspects,2025年:137250 ISSN：0927-7757
通讯作者：
Han Xu<&wdkj&>Yan Qing
作者机构：
[Yuxin Zhong; Jiahao Pi; Sha Chen; Pengcheng Liao; Yuanyuan Liao] College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China;State Key Laboratory of Utilization of Woody Oil Resource, Central South University of Forestry and Technology, Changsha 410004, PR China;[Jianbin Zeng] Hunan Aerospace TianLu advanced material testing Co., Ltd, Changsha 410004, PR China;[Han Xu; Yan Qing; Yiqiang Wu] College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China<&wdkj&>State Key Laboratory of Utilization of Woody Oil Resource, Central South University of Forestry and Technology, Changsha 410004, PR China
通讯机构：
[Han Xu; Yan Qing] C;College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, PR China<&wdkj&>State Key Laboratory of Utilization of Woody Oil Resource, Central South University of Forestry and Technology, Changsha 410004, PR China
摘要：
Manganese-based oxides, regarded as potential cathode materials for aqueous zinc-ion batteries (AZIBs), face significant challenges in their development and application, primarily due to their low electrical conductivity and inadequate electrochemical activity. Incorporating carbon as a substrate material represents a promising approach for enhancing the conductivity. Herein, bimetallic organic framework precursors were synthesized by hydrothermal method, with wood fibers serving as the carrier. The resulting carbonized wood fibers-supported carbon-coated MnO(MnO@C/CWF) was obtained through high-temperature calcination of the precursors. The carbon coating with a porous structure enhances the conductivity and accelerates the ion transport kinetics of manganese-based oxides but also improves their surface adsorption capacity and structural stability. Consequently, the specific capacity of MnO@C/CWF was 307 mAh g -1 at a current density of 0.2 A g -1 . Furthermore, MnO@C/CWF demonstrates long cycling stability, retaining 82% of its initial capacity after 10,000 cycles at 2 A g -1 . This work presents a strategy to enhance the electrochemical performance of MnO and advances the use of biomass-derived carbon fiber materials in aqueous zinc-ion batteries.
Manganese-based oxides, regarded as potential cathode materials for aqueous zinc-ion batteries (AZIBs), face significant challenges in their development and application, primarily due to their low electrical conductivity and inadequate electrochemical activity. Incorporating carbon as a substrate material represents a promising approach for enhancing the conductivity. Herein, bimetallic organic framework precursors were synthesized by hydrothermal method, with wood fibers serving as the carrier. The resulting carbonized wood fibers-supported carbon-coated MnO(MnO@C/CWF) was obtained through high-temperature calcination of the precursors. The carbon coating with a porous structure enhances the conductivity and accelerates the ion transport kinetics of manganese-based oxides but also improves their surface adsorption capacity and structural stability. Consequently, the specific capacity of MnO@C/CWF was 307 mAh g -1 at a current density of 0.2 A g -1 . Furthermore, MnO@C/CWF demonstrates long cycling stability, retaining 82% of its initial capacity after 10,000 cycles at 2 A g -1 . This work presents a strategy to enhance the electrochemical performance of MnO and advances the use of biomass-derived carbon fiber materials in aqueous zinc-ion batteries.
