期刊论文

Isolation of chitin deacetylase-producing microorganisms from riverside soils of Xiangjiang of China

中文

微生物学通报

0253-2654

2016

43

5

1019-1026

10.13344/j.microbiol.china.150802

(No.12K069):Scientific Research Fund of Hunan Provincial Education Department of China (No.NCET-13-1046):New Century Excellent Talents in University (No.2014-04)湖南省高校创新平台开放基金项目(No.12K069):Discipline Leaders in Universities Training Fund of Hunan Province (No.NCET-13-1046):教育部“新世纪优秀人才支持计划”基金项目 (No.2014-04):湖南省高校学科带头人培养对象基金项目

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【目的】甲壳素脱乙酰酶(CDA)是将天然甲壳素生物转化为可商品化利用的壳聚糖的关键酶。本文旨在从湘江河岸的土壤中筛选可高产CDA的新菌株。【方法】以甲壳素为唯一碳源,利用4'-硝基乙酰苯胺为显色剂,通过变色圈法进行产CDA菌株初筛,产酶活性分析复筛;通过形态学和ITS区序列特征对菌株进行鉴定。【结果】从湘江(长沙段)河岸边的土壤中分离出的117株菌株中筛选到可产CDA的菌株30株,其中4株具有较强产CDA的能力。进一步经发酵产酶分析验证,菌株A1具有较强的产CDA能力,其胞外CDA酶活高达13.21 U/mL。结合形态学和ITS区序列特征,菌株A1初步鉴定为层生镰孢菌。【结论】从湘江河岸边的土壤中筛选到可...

[Objective] Chitin deacetylase is a key enzyme in the conversion of natural chitin into chitosan, which can be commercially used. The aim of this work is to screen a new strain with high capacity for producing chitin deacetylase. [Methods] Chitin deacetylase producing strains from soils of Xiangjiang riverside in Changsha area were preliminarily screened with chitin as only source of carbon by color reaction of 4'-nitroacetanilide, and further screened by enzyme activity analysis; Identification of strains was analyzed by morphological and internal transcribed spacer (ITS) sequence. [Results] ...