期刊论文

中文

菌物学报

1672-6472

2016

35

9

1139-1150

10.13346/j.mycosystema.150155

(2012-4-11):国家948计划 (NCET-13-1046):教育部“新世纪优秀人才支持计划”基金 (2015JJ1025):湖南省自然科学***基金 (12K069,14K112):湖南省高校创新平台开放基金 :Supported by National "948" Project of China (2012-4-11),the Program for New Century Excellent Talents in University (NCET-13-1046),the Hunan Provincial Natural Science Foundation of China for Distinguished Young Scholars (2015JJ1025),and the Scientific Research Fund of Hunan Provincial Education D

本校为第一机构

比较了热水回流法、热水‐酶回流法、超声波法以及超声波‐酶水解法等4种提取方法对灵芝菌丝体中β‐葡聚糖提取得率的影响。结果表明,采用超声波‐酶水解法所得β‐葡聚糖的得率最大,影响提取的关键因素为超声波功率(A),水料比(B),pH(C)和加酶量(D)。进一步采用Box‐Behnken设计及响应面分析法对这4个因素进行了优化。结果显示,因素A、B、C对β‐葡聚糖提取得率的影响达到极显著水平(P0.05)。通过回归拟合,建立了预测灵芝β‐葡聚糖提取的多项式模型。经响应面最优分析,获得4个因素的最佳水平为:超声波功率275.54W,水料比25.62:1(V/M,mL/g),pH 7.17,加酶量1.05%。经实际试验验证,在该条件下,灵...

The four extraction methods, hot water reflux, hot water-enzyme reflux, ultrasonic, and ultrasonic combined with enzyme hydrolysis, were compared for β-glucan extraction from Ganoderma lingzhi mycelia. The results showed that the ultrasonic combined with enzyme hydrolysis was the most efficient method. Single factor experiment reveals that the key parameters affecting β-glucan yield of G. lingzhi were ultrasonic power（A）, ratio of water to material（B）, p H（C） and enzyme concentration（D）. Based on the results, Box-Benhnken design combi...