期刊论文

Extraction of β-glucan from Ganoderma lingzhi using ethanol-enzyme system pretreatment combined with typical water extraction method

中文

菌物学报

1672-6472

2016

35

1

104-113

10.13346/j.mycosystema.140186

国家“948”计划项目（2012-4-11） 教育部“新世纪优秀人才支持计划”基金（NCET-13-1046） 湖南省自然科学***基金（2015JJ1025） 湖南省高校创新平台开放基金（14K112）~~

本校为第一机构

采用乙醇-酶预处理体系,结合水提法较系统地研究了灵芝子实体中β-葡聚糖的提取技术。结果表明,乙醇-酶体系预处理的关键参数为:乙醇质量分数60%(V/V),加酶量1.5%(M/V,g/mL)、酶解温度45℃、酶解pH8.0;在乙醇-酶体系预处理的基础上,进一步采用单因素试验和Box-Benhnken试验设计与响应面分析法对灵芝子实体中β-葡聚糖的热水提取工艺进行了优化。结果显示水提温度、提取时间、水料比3个因素及水提温度与水料比二者的交互作用对β-葡聚糖的提取有显著影响。经优化后获得3个核心因素的最佳水平为:提取温度80℃、提取时间2.5h、水料比35:1。在乙醇-酶预处理结合水提取条件下,灵芝子实体中β-葡聚糖的...

β-glucans from Ganoderma lingzhi fruiting bodies were extracted using ethanol-enzyme pretreatment combined with the typical water extraction method. The results showed that the key parameters of ethanol-enzyme pretreatment system were 60% of ethanol (V/V), 1.5% of enzyme (M/V, g/mL), 45°C of enzymolysis temperature and 8.0 of enzymolysis pH. Based on the ethanol-enzyme pretreatment method, the hot water extraction technology of β-glucans was further optimized using single factor test and Box-Behnken test design. The results revealed that the...