作者： Ouyang, Qunfu;Xiao, Jiaqi;Chen, Jialin;Xiao, Yiwei;Lin, Qinlu;...

期刊： STARCH-STARKE,2021年73(1-2):1900317- ISSN：0038-9056

通讯作者： Ding, Yongbo

作者机构： [Ding, Yongbo; Ouyang, Qunfu; Xiao, Jiaqi; Xiao, Yiwei; Chen, Jialin; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Prod Deep Proc, Hunan Key Lab Proc Food Special Med Purpose, Coll Food Sci & Engn,Hunan Key Lab Grain Oil Deep, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Ding, Yongbo] C;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Prod Deep Proc, Hunan Key Lab Proc Food Special Med Purpose, Coll Food Sci & Engn,Hunan Key Lab Grain Oil Deep, Changsha 410004, Hunan, Peoples R China.

关键词： emulsification–physical technique;in vitro digestibility;in vitro release properties;nanoresistant starch particles;stability

摘要： <jats:title>Abstract</jats:title><jats:p>Nanoresistant particles based on chemically modified starch (RS IV) are prepared through a high‐speed shearing emulsification method, and the effects of shear speed, shear time, oil/water ratio, and surfactant amount on the particle size are evaluated in this study. The as‐prepared RS IV nanoparticles yield ≈300nm narrow peak with notches and grooves on the surface. In vitro digestibility results suggest that these nanoparticles exhibit high resistance in the stomach, small intestine, and colon contents of mice. A raman assay confirms that a crosslinking reaction occurs between the RSIV molecule and <jats:italic>N</jats:italic>,<jats:italic>N</jats:italic>′‐methylenediacrylamide. X‐ray diffraction (XRD) and differential scanning calorimetry (DSC) reveal that RS IV nanoparticles present an amorphous structure. In addition, the RS IV nanoparticles have good thermal and storage stabilities. In comparison with RS IV, RS IV nanoparticles have a larger specific surface area, smaller average pore size, higher loading capacity and encapsulation efficiency, and better in vitro release properties of drugs.</jats:p>

语种： 英文

作者： Xu, Weizhen;Wang, Boyang;Zhang, Yadan;An, Qingxiao;Zhou, Rongju;...

期刊： Materials Research Express,2021年8(3):032001 ISSN：2053-1591

通讯作者： Lin, Qinlu

作者机构： [An, Qingxiao; Peng, Chenzhan; Zhou, Rongju; Zhang, Yadan; Xu, Weizhen; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Prod Further Proc, Changsha 410004, Peoples R China.;[Xing, Yanxia; Wang, Boyang] Shandong Univ Agr Engn, Key Lab, Shandong Educ Dept, Jinan 250100, Peoples R China.

通讯机构： [Lin, Qinlu] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Prod Further Proc, Changsha 410004, Peoples R China.

关键词： Gold;Gold Nanorods;Molecular biology;Nanorods;Plasmons;Biological detection;Biological fields;Functionalized;Future prospects;Gold nanorods (AuNRs);Medical areas;Surface plasmon resonance

摘要： The development of new methods for applications of detection in the biological field is a topic of intense and growing interest. The currently practiced methods for biological detection have many defects, such as time-consuming, low accuracy, and cumbersome for operators. Gold nanorods (AuNRs) have received tremendous attention recently in the fields of biological detection owing to the unique characteristics of surface plasmon resonance (SPR), which provides a remarkable ability to absorb and scatter light. Furthermore, AuNRs can be functionalized with a wide variety of biomolecules for targeted detection. Moreover, their versatility and unique properties have generated more applications in medical areas. In this review, we briefly outline the synthesis and surface modification of AuNRs, and focus on the recent advances of the application of AuNRs in detection field. Finally, the outlook and future prospect on the development of AuNRs are provided. © 2021 The Author(s). Published by IOP Publishing Ltd

语种： 英文

作者： Wang, Long;Wang, Dandan;Yang, Zhuhong;Jiang, Shun;Qu, Jianing;...

期刊： 中国科学:生命科学(英文版),2021年64(2):294-310 ISSN：1674-7305

通讯作者： Yu, Feng;Lin, Qinlu

作者机构： [Wang, Long; Yang, Zhuhong; Yu, Feng; He, Wei] Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wang, Long; Yang, Zhuhong; Yu, Feng; He, Wei] Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.;[Wang, Long; Qu, Jianing; Jiang, Shun; Wang, Dandan; Liu, Zhenming; Ma, Youchu; He, Wei; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Changsha 410004, Peoples R China.;[Wang, Long; Yang, Zhuhong; Yu, Feng; Xing, Junjie] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China.

通讯机构： [Yu, Feng] H;[Lin, Qinlu] C;Hunan Univ, Coll Biol, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Funct Genom & Dev Regula, Changsha 410082, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Changsha 410004, Peoples R China.

关键词： FLR;chalkiness;seed size;grain quality;OsRac1

摘要： Grain yield and quality are critical factors that determine the value of grain crops. In this study, we analyzed the functions of 12 FERONIA-like receptor (FLR) family members in rice and investigated their effects on grain size and quality. We found that FLR1, FLR2 and FLR8 negatively regulated grain size, and FLR15 positively regulated grain size. flr1 mutants had a higher cell number and an accelerated rate of grain filling compared to wild-type plants, which led to grains with greater widths. A mechanism underlying the regulation of grain size by FLR1 is that FLR1 is associated with OsRac1 Rho-like GTPase, a positive regulator of grain size. Regarding grain quality, the flr1 mutant had a higher percentage of chalkiness compared with wild-type plants, and seeds carrying mutations in flr3 and flr14 had endosperms with white floury cores. To elucidate the possible mechanism underlying this phenomenon, we found that FLR1 was constitutively expressed during endosperm development. RNA-seq analysis identified 2,367 genes that were differentially expressed in the flr1 mutant, including genes involved in starch and sucrose metabolism and carbon fixation. In this study, we identified the roles played by several FLR genes in regulating grain size and quality in rice and provided insights into the molecular mechanism governing the FLR1-mediated regulation of grain size. © 2020, Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Shi, Yi;Zhang, Dan-Dan;Liu, Ji-Bin;Yang, Xiao-Li;Xin, Rui;...

期刊： Molecular Therapy - Nucleic Acids,2021年23:702-718 ISSN：2162-2531

通讯作者： Li, Wen;Da, Fu;Ma, Yu-Shui

作者机构： [Shi, Yi; Deng, Jing; Li, Zi-Jin; Li, Wen; Lin, Qin-Lu; Liu, Yu; Chen, Xiao-Qi; Da, Fu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Deep Proc Rice & Byprod, 498 South Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.;[Shi, Yi; Liu, Ji-Bin] Nantong Tumor Hosp, Canc Inst, Nantong 226631, Peoples R China.;[Shi, Yi; Ma, Liang; Zheng, Xiang-Min; Li, Wen; Feng, Shan-Shan; Liu, Yu] Hunan Univ Technol, Coll Life Sci & Chem, Zhuzhou 412007, Hunan, Peoples R China.;[Zhang, Dan-Dan] Shihezi Univ, Dept Pathol, Sch Med, Shihezi 832002, Xinjiang, Peoples R China.;[Yang, Xiao-Li; Wang, Pei-Yao; Xin, Rui; Zhang, Dan-Dan; Wang, Hui-Min; Gu, Li-Peng; Da, Fu; Tian, Lin-Lin] Tongji Univ, Shanghai Peoples Hosp 10, Cent Lab Med Res, Sch Med, 36 Yunxin Rd, Shanghai 200072, Peoples R China.

通讯机构： [Li, Wen] C;[Da, Fu; Ma, Yu-Shui] T;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Deep Proc Rice & Byprod, 498 South Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.;Tongji Univ, Shanghai Peoples Hosp 10, Cent Lab Med Res, Sch Med, 36 Yunxin Rd, Shanghai 200072, Peoples R China.;Tongji Univ, Shanghai Peoples Hosp 10, Dept Nucl Med, Sch Med, 36 Yunxin Rd, Shanghai 200072, Peoples R China.

关键词： argonaute 2 protein;dleu2l protein;fam99a protein;hs3st3b1 protein;long untranslated RNA;messenger RNA;microRNA;microRNA 100;microRNA 125b;microRNA 9;microRNA 99a;mitogen activated protein kinase;nuclear receptor NR6A1;phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase;rhoq protein;taok1 protein;tumor marker;unclassified drug;Article;cancer prognosis;cell nucleus;clinical article;cytoplasm;down regulation;human;human tissue;immunohistochemistry;liver cell carcinoma;lymphocytic infiltration;methylation;overall survival;priority journal;RNA extraction;tumor microenvironment;upregulation

摘要： Shi et al. provide evidence that a ceRNA-based DLEU2L/TAOK1 axis has an impact on changes in the tumor immune microenvironment and might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC. © 2020 The Author(s)Hepatocellular carcinoma (HCC) is one of the deadliest malignant tumors that are harmful to human health. Increasing evidence has underscored the critical role of the competitive endogenous RNA (ceRNA) regulatory networks among various human cancers. However, the complexity and behavior characteristics of the ceRNA network in HCC were still unclear. In this study, we aimed to clarify a phosphatase and tensin homolog (PTEN)-related ceRNA regulatory network and identify potential prognostic markers associated with HCC. The expression profiles of three RNAs (long non-coding RNAs [lncRNAs], microRNAs [miRNAs], and mRNAs) were extracted from The Cancer Genome Atlas (TCGA) database. The DLEU2L-hsa-miR-100-5p/ hsa-miR-99a-5p-TAOK1 ceRNA network related to the prognosis of HCC was obtained by performing bioinformatics analysis. Importantly, we identified the DLEU2L/TAOK1 axis in the ceRNA by using correlation analysis, and it appeared to become a clinical prognostic model by Cox regression analysis. Furthermore, methylation analyses suggested that the abnormal upregulation of the DLEU2L/TAOK1 axis likely resulted from hypomethylation, and immune infiltration analysis showed that the DLEU2L/TAOK1 axis may have an impact on the changes in the tumor immune microenvironment and the development of HCC. In summary, the current study constructing a ceRNA-based DLEU2L/TAOK1 axis might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC. © 2020 The Author(s)

语种： 英文

作者： Ding, Yongbo;Liang, Ying;Luo, Feijun;Ouyang, Qunfu;Lin, Qinlu*

期刊： Carbohydrate Polymers,2020年228:115350 ISSN：0144-8617

通讯作者： Lin, Qinlu

作者机构： [Ding, Yongbo; Luo, Feijun; Ouyang, Qunfu; Liang, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Natl Engn Lab Rice & Byprod Deep Proc,Hunan Key L, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Lin, Qinlu] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Natl Engn Lab Rice & Byprod Deep Proc,Hunan Key L, Changsha 410004, Hunan, Peoples R China.

关键词： In vitro digestibility;Regulation;Resistant starch;Structural properties;Ultrasonic treatment

摘要： The digestibility properties and structural changes of retrograded starch (RS3) induced by ultrasonic treatment (UT) were investigated. The digestion profiles showed that UT increased the slowly digestible starch (SDS) or resistant starch (RS) of RS3 as an effective green process, corresponding to a change in hydrolysis kinetic parameters (equilibrium starch hydrolysis percentage and kinetic constant). SEM analysis showed that ultrasound led to breakage of RS3 particles followed by cracking, reorientation and crystallization. Differences in amylose content, granule size, and ζ-potential were found for native RS3 and ultrasound-treated RS3 (UT-RS3). UT decreased the relative crystallinity and gelatinization enthalpy but enhanced short-range order of RS3 based on the results of XRD, DSC, and FT-IR, respectively. Surprisingly, diffractive peaks at 13°and 20° (V-type crystalline structure) and a new exothermic peak were also observed for UT-RS3. The outcome was believed to open new pathways for regulating the digestibility properties of RS3 by UT and development of low glycemic response food. © 2019 Elsevier Ltd

语种： 英文

作者： Ding, Haiyuan;Yuan, Gangqiang;Peng, Longpeng;Zhou, Liyi*;Lin, Qinlu

期刊： JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2020年68(11):3670-3677 ISSN：0021-8561

通讯作者： Zhou, Liyi

作者机构： [Yuan, Gangqiang; Zhou, Liyi; Peng, Longpeng; Ding, Haiyuan; Lin, Qinlu] Cent South Univ Forestry & Technol, Hunan Key Lab Grain Oil Drip Proms & Qual Control, Natl Engn Lab Deep Proc Rice & Byprod,Coll Food S, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhou, Liyi] C;Cent South Univ Forestry & Technol, Hunan Key Lab Grain Oil Drip Proms & Qual Control, Natl Engn Lab Deep Proc Rice & Byprod,Coll Food S, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.

关键词： Energy transfer;Formaldehyde;Histology;Photons;Probes;Tissue;Fluorescence resonance energy transfer;Fluorescent probes;highly reactive carbonyl species;Pathological functions;Push-pull structures;Ratiometric detection;Ratiometric imaging;Zebrafish;Forster resonance energy transfer;fluorescent dye;formaldehyde;animal;fluorescence resonance energy transfer;HeLa cell line;human;zebra fish;Animals;Fluorescence Resonance Energy Transfer;Fluorescent Dyes;Formaldehyde;HeLa Cells;Humans;Zebrafish

摘要： Formaldehyde (FA, HCHO) is a highly reactive carbonyl species, which is very harmful to humans and the environment as a tissue fixative and preservative. Therefore, developing some highly sensitive, selective, and rapid detection methods is significant for human health in food safety and environmental protection. Herein, a two-photon (TP) ratiometric sensor, CmNp-CHO, has been constructed by conjugating a TP donor (Î -push-pull-structure) with a FA off-on acceptor (functioned with hydrazide moiety) via a nonconjugated linker through the fluorescence resonance energy transfer mechanism. Such a scaffold affords CmNp-CHO a reliable and specific probe for detecting FA with two well-resolved emission peaks separated by 124 nm. Also, it responds to FA rapidly with high selectivity and sensitivity during 1.0 min and a large ratio enhancement at I550/I426 with addition of 0-20μM FA, exhibiting 4-fold ratio increase and a fairly low LOD of 8.3 ± 0.3 nM. Moreover, CmNp-CHO has been successfully employed for detecting FA in live cells, onion tissues, and zebrafish, exhibiting that CmNp-CHO can serve as a useful tool for investigating FA in real food application and offering strong theoretical support and technical means for investigation of physiological and pathological functions of FA. Copyright © 2020 American Chemical Society.

语种： 英文

作者： Tang, Wenjie;Wu, Jian;Jin, Shunshun;He, Liuqin*;Lin, Qinlu;...

期刊： 中国科学:生命科学(英文版),2020年63(1):116-124 ISSN：1674-7305

通讯作者： He, Liuqin;Li, Tiejun

作者机构： [Yin, Yulong; Tang, Wenjie; He, Liuqin] Hunan Normal Univ, Coll Life Sci, Lab Anim Nutr & Human Hlth, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.;[Wu, Jian; Li, Tiejun; Yin, Yulong; He, Liuqin; Jin, Shunshun] Chinese Acad Sci, Hunan Prov Key Lab Anim Nutrit Physiol & Metab Pr, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China.;[Luo, Feijun; Lin, Qinlu; He, Liuqin] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Proc Food Special Med Purpose, Natl Engn Lab Deep Proc Rice & Byprod,Hunan Key L, Changsha 410004, Peoples R China.;[He, Binsheng; Bing, Pingping] Changsha Med Univ, Affiliated Hosp 1, Acad Working Stn, Changsha 410219, Peoples R China.;[Tang, Wenjie] Sichuan Acad Anim Sci, Anim Breeding & Genet Key Lab Sichuan Prov, Chengdu 610066, Peoples R China.

通讯机构： [He, Liuqin] H;[He, LQ; Li, TJ; He, Liuqin] C;Hunan Normal Univ, Coll Life Sci, Lab Anim Nutr & Human Hlth, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.;Chinese Acad Sci, Hunan Prov Key Lab Anim Nutrit Physiol & Metab Pr, Natl Engn Lab Pollut Control & Waste Utilizat Liv, Key Lab Agroecol Proc Subtrop Reg, Changsha 410125, Peoples R China.;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Proc Food Special Med Purpose, Natl Engn Lab Deep Proc Rice & Byprod,Hunan Key L, Changsha 410004, Peoples R China.

关键词： glutamate;aspartate;boars;oxidative stress;inflammatory cytokines

摘要： Several potential oxidative agents have damaging effects on mammalian reproductive systems. This study was conducted to investigate the effects of glutamate (Glu) and aspartate (Asp) supplementation on antioxidant enzymes and immune defense systems in the outer scrotum of boars injected with H 2 O 2 . A total of 24 healthy boars were randomly divided into 4 treatment groups: control (basal diet, saline-treated), H 2 O 2 (basal diet, H 2 O 2 -challenged outer scrotum (1 mL kg −1 BW)), Glu (basal diet +2% Glu, H 2 O 2 -challenged), and Asp (basal diet+2% Asp, H 2 O 2 -challenged). Our results showed that both Glu and Asp supplementation improved testicular morphology and decreased the genital index in the H 2 O 2 -treated boars. Glu and Asp administration increased the antioxidant enzyme activities and affected the testicular inflammatory cytokine secretion but had no effect on sex hormone levels. Furthermore, the mRNA expression of CAT, CuZnSOD, and GPx4 was altered in the testes and epididymis of boars treated with Asp and Glu. Glu and Asp supplementation also modulated the expression of TGF-β1, IL-10, TNF-α, IL-6 and IL-1β in the testis and epididymis. These results indicate that dietary Glu and Asp supplementation might enhance antioxidant capacity and regulate the secretion and expression of inflammatory cytokines to protect the testes and epididymis of boars against oxidative stress. © 2019, Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.

语种： 英文

作者： Yuan, Gangqiang;Ding, Haiyuan;Peng, Longpeng;Zhou, Liyi*;Lin, Qinlu*

期刊： Food Chemistry,2020年331:127221 ISSN：0308-8146

通讯作者： Zhou, Liyi;Lin, Qinlu

作者机构： [Yuan, Gangqiang; Zhou, Liyi; Peng, Longpeng; Zhou, LY; Lin, Qinlu; Ding, Haiyuan] Cent South Univ Forestry & Technol, Hunan Key Lab Processed Food Special Med Purpose, Hunan Key Lab Grainoil Deep Proc & Qual Control, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha 410004, Hunan, Peoples R China.

通讯机构： [Zhou, LY; Lin, QL] C;Cent South Univ Forestry & Technol, Hunan Key Lab Processed Food Special Med Purpose, Hunan Key Lab Grainoil Deep Proc & Qual Control, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha 410004, Hunan, Peoples R China.

关键词： FRET probe;Fluorescent probe;Formaldehyde (FA);Two-photon ratiometric imaging

摘要： Herein, a two-photon (TP) ratiometric fluorescent probe (NpFA) was developed for detecting formaldehyde (FA) in real food samples, living onion tissues and zebrafish by fluorescence resonance energy transfer (FRET) strategy. Specifically, a TP fluorophore as the donor and a FA turn-on naphthalimide fluorophore as the acceptor were connected by a non-conjugated linker to construct the TP-FRET-based NpFA, which exhibited a target-modulated ratiometric fluorescence response to FA rapidly with high selectivity and sensitivity during 65 s, and a large ratio ~5-fold enhancement at I550/I410 after addition of FA, displaying ~60-fold enhancement at 550 nm and a quite low DOC of 5.8 ± 0.2 nM. Moreover, NpFA has a good imaging resolution and depth of deep tissue penetration. Therefore, based on the above results, NpFA has the capability to be a useful tool for investigating FA in real samples application, and we also hope NpFA will further study of the physiological and pathological function of FA. © 2020 Elsevier Ltd

语种： 英文

作者： 苗兰鸽;许燕;赵思明;贾才华;牛猛;...

期刊： 食品工业科技,2020年41(14):22-28 ISSN：1002-0306

作者机构： 华中农业大学食品科学技术学院;[林亲录] 中南林业科技大学;[贾才华; 赵思明; 苗兰鸽; 许燕; 牛猛] 华中农业大学

关键词： 花青素;淀粉;结合能力;相互作用

摘要： 低、中、高直链大米淀粉为原料,通过添加不同比例(0％、1％、2％、5％和10％)的花青素,研究花青素对淀粉理化特性的影响.结果 表明,随着花青素含量的增加,淀粉的碘结合能力、表观黏度和黏弹性均降低,且这种降低趋势与直链淀粉含量相关.随着花青素含量的增加,糊化温度、焓值逐渐降低,当花青素添加量为10％时,三种体系的糊化焓值分别下降23.92％、44.91％和59.89％;添加花青素的淀粉红外图谱与原淀粉相似,但在3400 cm-1附近的吸收峰红移.此外,淀粉的结晶度随花青素的添加而呈现降低的趋势,当花青素添加量为10％时,三种体系的结晶度相对于原淀粉分别下降7.99％、15.49％和24.87％,通过扫描电镜的结果表明,添加花青素后淀粉的微观形貌呈现疏松多孔的性质.综上表明,花青素对淀粉的理化性质有明显的影响,且这一影响与淀粉中直链淀粉含量相关.本文通过研究花青素对淀粉理化特性的影响,可以为花青素在淀粉中的应用提供一定的理论依据.

语种： 中文

作者： 肖怀秋;李玉珍;赵谋明;林亲录;刘军;...

期刊： 食品工业科技,2020年41(2):114-120 ISSN：1002-0306

作者机构： 湖南化工职业技术学院制药与生物工程学院;华南理工大学食品科学与工程学院;中南林业科技大学食品科学与工程学院;[姜明姣] 湖南中威制药有限公司;[刘军; 肖怀秋; 李玉珍; 周全] 制药与生物工程学院

关键词： 枯草芽孢杆菌;益生菌;喷雾干燥;菌体存活率;含水量

摘要： 为优化枯草芽孢杆菌喷雾干燥工艺参数,以菌体存活率为评价指标,利用单因素实验研究了保护剂用量、入口温度、进料速率、进风量和出口温度对菌体存活率的影响.结果 表明,进料速率、进风量和出口温度为重要因素;采用中心复合响应面优化技术研究了进料速率(X1)、进风量(X2)、出口温度(X3)对菌体存活率的影响并构建了多元非线性回归方程;方差与回归分析表明,进料速率(X1)与进风量(X2)影响不显著(P＞0 05),出口温度(X3)影响极显著(P＜0.01).喷雾干燥最优参数为进料速率600 mL/h,进风量80 m3/h和出口温度52℃,优化条件下验证试验结果为96.64％±1.43％,与模型预测值基本一致.试验结果表明,热诱激预处理和复合抗热保护剂的使用可提高菌体热耐受性,显著提高喷雾干燥后菌体存活率,研究结果可为枯草芽孢杆菌的喷雾干燥提供理论支持.

语种： 中文

作者： 王静;陈晨;李楠楠;贾才华;赵思明;...

期刊： 中国粮油学报,2020年35(7):8-14 ISSN：1003-0174

通讯作者： Jia, C.

作者机构： [王静; 陈晨; 李楠楠; 贾才华; 赵思明; 张宾佳; 牛猛; 熊善柏] College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;[林亲录] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410004, China

通讯机构： College of Food Science and Technology, Huazhong Agricultural University, Wuhan, China

关键词： 米发糕;发酵菌株;抗氧化活性;水解肽

摘要： 以籼米粉为原料,三种发酵剂(酵母菌ZSM-001;酵母菌ZSM-001与米根霉ZSM-003;安琪酵母与酒曲发酵剂)制作米发糕,通过凝胶色谱测定其水提物和胃肠水解物分子质量分布,基于液相色谱-四级杆飞行时间质谱(TOF LC/MS/MS)研究肽的差异性,并进行胃肠水解物的抗氧化活性评价。结果表明,米发糕经胃肠水解后,其抗氧化活性提升,分子质量集中在3 ku以下,小分子组分(分子质量<3 ku)的Fe~(2+)螯合能力和还原能力较其他组分高。发酵剂组合对米发糕胃肠水解物的肽段数量、疏水性和抗氧化性有显著影响,以安琪酵母与酒曲复合发酵制备米发糕的胃肠水解物中分子质量小于3 ku组分的肽段数最多,疏水性较强,且Fe~(2+)螯合能力最大,还原能力较好。

语种： 中文

作者： 肖怀秋;李玉珍;林亲录;刘军;姜明姣;...

期刊： 食品与发酵工业,2020年46(18):111-116 ISSN：0253-990X

作者机构： 湖南化工职业技术学院制药与生物工程学院;中南林业科技大学食品科学与工程学院;[姜明姣] 湖南中威制药有限公司;华南理工大学食品科学与工程学院;[刘军; 肖怀秋; 李玉珍] 制药与生物工程学院

关键词： 花生肽亚铁;金属肽;胃肠仿生消化产物;食品抗菌肽;抑菌机理

摘要： 为探讨花生肽亚铁胃肠仿生消化产物作为抑菌剂的潜在可能性,从抑菌活性、表面疏水性、最低抑菌浓度(minimum inhibitory concentration,MIC) 、细胞膜渗透性、紫外吸收大分子物质和K +泄漏等方面进行了研究。结果表明,胃肠仿生消化能增强花生肽亚铁抑菌活性;仿生消化270 min时表面疏水值最大;小肠仿生消化产物(simulated intestinal fragment,SIF4)为0.2 × 10 ~(- 3) g/L时(MIC)对金黄色葡萄球菌有明显抑菌作用;试验组细胞膜渗透性、蛋白质和核酸等紫外吸收物质泄露与对照组均有显著差异(P<0.05) 。经1/2 MIC、1/4 MIC和MIC处理后,金黄色葡萄球菌胞内K +的泄露随温育时间延长呈递增趋势,特别是MIC组。研究认为,金黄色葡萄球菌经SIF4处理后,细胞膜完整性遭到破坏而造成膜穿孔,使胞内蛋白质与核酸等生物活性物质外泄并造成菌体坏死,起到良好抑菌效果。因此,花生肽亚铁经胃肠仿生消化后可作为一种潜在的新型多肽金属抑菌剂应用于食品领域。

语种： 中文

作者： Sun, Shuguo;Zhao, Juanhong;Luo, Zhang;Lin, Qinlu;Luo, Feijun;...

期刊： Journal of Food Science and Technology,2020年57(4):1351-1361 ISSN：0022-1155

通讯作者： Yang, Tao

作者机构： [Luo, Feijun; Yang, Tao; Zhao, Juanhong; Sun, Shuguo; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha Engn Res Ctr Food Storage & Preservat,Hu, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;[Luo, Zhang] Tibet Agr & Anim Husb Univ, Coll Food Sci, Lingzhi 860000, Tibet, Peoples R China.;[Luo, Feijun] Univ Cambridge, Addenbrookes Hosp, Dept Pathol, Cambridge, England.

通讯机构： [Yang, Tao] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha Engn Res Ctr Food Storage & Preservat,Hu, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.

关键词： Yak meat;Deterioration index;Volatile compounds;Headspace solid-phase microextraction

摘要： In this study, the physicochemical properties (total volatile basic nitrogen (TVB-N), pH, and peroxide value) and the volatile flavors of yak meat were systematically evaluated during chilled (0 degrees C) and controlled freezing-point (- 2 degrees C) storage. The TVB-N reached 15.21 mg/100 g after 18 days of storage at 0 degrees C, which exceeded the secondary freshness value according to the Chinese national standard. For storage at - 2 degrees C, the TVB-N did not exceed 15 mg/100 g until 24 days. Compared with storage at 0 degrees C, the samples stored at - 2 degrees C had a slower rate of increase in TVB-N, pH, and peroxide values. The changes in volatile compounds in yak meat during storage at - 2 degrees C and 0 degrees C for 24 days were investigated using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS). The correlations between the changes in the volatile compound contents and meat quality deterioration revealed significant negative correlations (r(min) = 0.902, p < 0.05) between some aldehyde flavor components (nonanal, heptanal, benzaldehyde, decanal, and myristal) and TVB-N in the samples stored at controlled freezing-point and chilled temperatures. The decreases in nonanal, benzaldehyde, and myristal contents in yak meat followed zero order reaction kinetics. This result indicated, because of the highly selective and sensitive colorimetric detection method, that volatile compounds can effectively predict the decay in quality of yak meat stored at low temperature in advance. Thus, based on physicochemical and volatile flavor analyses, a new method is proposed to investigate the storage and preservation of yak meat.

语种： 英文

作者： Ma, Yu-Shui;Wang, Xiao-Feng;Yu, Fei;Wu, Ting-Miao;Liu, Ji-Bin;...

期刊： 信号转导与靶向治疗,2020年5(1):417-419 ISSN：2095-9907

通讯作者： Fu, Da;Jiang, Zong-Yuan;Lin, Qin-Lu

作者机构： [Fu, Da; Ma, Yu-Shui] Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Cent Lab Med Res, Shanghai 200072, Peoples R China.;[Ma, Yu-Shui; Liu, Ji-Bin] Nantong Tumor Hosp, Canc Inst, Nantong 226631, Peoples R China.;[Yu, Fei; Ma, Yu-Shui] Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Nucl Med, Shanghai 200072, Peoples R China.;[Wu, Ting-Miao; Fu, Da; Ma, Yu-Shui] Anhui Med Univ, Affiliated Hosp 4, Dept Radiol, Hefei 230012, Peoples R China.;[Zhang, Yun-Jie; Xia, Qing; Wang, Xiao-Feng] Fudan Univ, Zhongshan Hosp, Dept Orthoped, Shanghai 200032, Peoples R China.

通讯机构： [Fu, Da] T;[Fu, Da] A;[Jiang, Zong-Yuan] S;[Lin, QL; Fu, D] C;Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Cent Lab Med Res, Shanghai 200072, Peoples R China.

摘要： Dear Editor,Tumor-suppressor protein p53 is important for cell function and genome integrity.1 The decrease of p53 protein is a common feature of human malignant tumors,which leads to the deficiency of cell cycle detection point control and apoptosis induction.An enormous amount of research effort goes into small molecules that regulate p53,including wild-type repair of mutant p53 gene and interruption of the binding between p53 and an E3-ubiquitin ligase Mdm2 to prevent ubiquitination degradation of p53 and rescue the protein level of p53.2 However,it is not clear whether it is necessary to continue to inhibit the ubiquitin activity of proteasome in order to restore the p53 protein level.

语种： 英文

作者： Li, Nannan;Wang, Lili;Zhao, Siming;Qiao, Dongling;Jia, Caihua;...

期刊： Food Hydrocolloids,2020年103:105690 ISSN：0268-005X

通讯作者： Zhang, Binjia

作者机构： [Wang, Lili; Niu, Meng; Zhang, Binjia; Jia, Caihua; Li, Nannan; Zhao, Siming] Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Grp Cereals & Oils Proc,Minist Educ, Wuhan 430070, Peoples R China.;[Qiao, Dongling] Hubei Univ Technol, Glyn O Phillips Hydrocolloid Res Ctr HBUT, Sch Food & Biol Engn, Wuhan 430068, Peoples R China.;[Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Prod Deep Proc, Changsha 410004, Peoples R China.

通讯机构： [Zhang, Binjia] H;Huazhong Agr Univ, Coll Food Sci & Technol, Key Lab Environm Correlat Dietol, Grp Cereals & Oils Proc,Minist Educ, Wuhan 430070, Peoples R China.

关键词： Digestion feature;Microwave cooking;Multi-scale structural evolution;Starch

摘要： The rice starch following microwave cooking with storage showed more slowly digestible starch and a lower digestion rate than did the conventionally treated counterpart. The underlying mechanism was interpreted by inspecting starch multi-level structural evolutions during digestion. Accompanying digestion, not only were starch matrices digested, leading to porous substrate and probably less polymorphs and nanoscale orders, but also starch chain reassembly occurred, causing A to B crystalline transform for untreated starch and formation of new molecular organization (repeat length: 5 nm) for treated starches. Hence, the digestion for native and treated starches was governed by concurrent matrix hydrolysis and molecular reassembly during digestion. The ultimate digestion of a specific structure was affected by the state of structural system. Unlike common views, the polymorphs in processed starches without native architecture were preferentially digested. Also, compared to conventionally treated counterpart, the microwave treated starch exhibited enhanced molecular reassembly during digestion, eventually displaying stronger slowly digestible features. © 2020 Elsevier Ltd

语种： 英文

作者： Li, Wang;Chen, Zihao;Ren, Jiali;Yang, Tao*;Lin, Qinlu*;...

期刊： Sensors and Actuators B-Chemical,2020年304:127283 ISSN：0925-4005

通讯作者： Yang, Tao;Lin, Qinlu;Liu, Juewen

作者机构： [Chen, Zihao; Yang, Tao; Lin, Qinlu; Ren, Jiali; Li, Wang] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;[Li, Wang] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Tao; Lin, Qinlu] Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;[Liu, Juewen] Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G1, Canada.;[Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Waterloo, ON N2L 3G1, Canada.

通讯机构： [Yang, T; Lin, QL] C;[Liu, Juewen] U;Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G1, Canada.

关键词： Nanoceria;Cr(3+)detection;Electric resistant;Multimeter;3D printed portable device

摘要： Using simple and commonly available devices for signal reading has attracted much attention recently for DNA-based sensors. Most previous works focused on electrochemical or colorimetric signals. In this work, a portable device was structured by simple electronic and 3D-printed components to convert fluorescence signal to electric resistance with reading by a multimeter. To demonstrate its application, we took advantage of the slow ligand bind kinetics of Cr3+ to promote DNA adsorption on metal oxide nanoparticles (MONPs). Nanoceria (CeO2), screened from a total of 13 kinds of MONPs, possesses high specificity for Cr3+ from other metal ions. EDTA can mask other metal ions, while it cannot bind Cr3+ quickly so that Cr3+ promotes DNA adsorption and thus fluorescence quenching. The linear range is from 0 to 2 mu M Cr3+ with a LOD of 0.02 mu M. Using common electronic components and the 3D-printed miniaturized device, the sensor to transfer fluorescence to electric resistant signal was also measured by a digital multimeter. In this case, the LOD is about 1 mu M Cr3+. Such a strategy may provide a simple and general approach for fluorescent portable device fabrication and may find applications for other onsite sensing applications.

语种： 英文

作者： Liu, Jun;Luo, Yunchuan;Guo, Ting;Tang, Chenglun;Chai, Xueying;...

期刊： Journal of Bioscience and Bioengineering,2020年129(2):229-236 ISSN：1389-1723

通讯作者： Lin, Qinlu

作者机构： [Guo, Ting; Chai, Xueying; Bai, Jie; Zhao, Wen; Liu, Jun; Luo, Yunchuan; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Natl Engn Lab Deep Proc Rice & Byprod,Hunan Key L, Changsha 410004, Hunan, Peoples R China.;[Liu, Jun] Minist Agr & Rural Affairs Peoples Republ China, Key Lab Staple Grain Proc, Zhengzhou 450002, Henan, Peoples R China.;[Tang, Chenglun] Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, 30 Puzhu South Rd, Nanjing 211816, Peoples R China.

通讯机构： [Lin, Qinlu] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Natl Engn Lab Deep Proc Rice & Byprod,Hunan Key L, Changsha 410004, Hunan, Peoples R China.

关键词： Aldehydes;Biochemistry;Cost effectiveness;Fermentation;Fits and tolerances;Furfural;Glucose;Metabolites;Methanol;Textile industry;Adaptation mechanism;Glucose fermentation;Hydroxymethyl furfural;Monascus pigment;Rice straw hydrolysate;Secondary metabolites;Submerged fermentation;Temperature plasmas;Substrates;5 hydroxymethylfurfural;furfural;starch hydrolysate;glucose;pigment;adaptation;agricultural waste;air temperature;Article;bacterial strain;biosynthesis;controlled study;cost effectiveness analysis;extracellular matrix;hydrolysis;microbial growth;Monascus purpureus;mutagenesis;nonhuman;pigmentation;room temperature;submerged fermentation;biosynthesis;cost benefit analysis;economics;enzyme specificity;fermentation;metabolism;Monascus;Oryza;Cost-Benefit Analysis;Fermentation;Glucose;Hydrolysis;Monascus;Oryza;Pigments, Biological;Substrate Specificity

摘要： Monascus pigments (MPs), the secondary metabolites produced by the fungal strains of Monascus spp., hold commercial importance in not only the food and meat industries, but also therapeutic, cosmetic, and textile industries. To reduce the cost of MPs production, the utilization of rice straw hydrolysate as a substrate in submerged fermentation was investigated. The atmospheric and room temperature plasma (ARTP) mutation system was employed to develop a mutant strain Monascus purpureus M630, with high total extracellular Monascus pigments (exMPs) production of 34.12 U/mL in submerged fermentation with glucose-based medium. The results revealed that M. purpureus M630 produces 8.61 U/mL and 20.86 U/mL of exMPs in rice straw hydrolysate alone or in combination with glucose fermentation medium, respectively. Furfural (Fur) and 5'-hydroxymethyl furfural (5'-HMF), produced during pretreatment and hydrolysis of rice straw; are generally inhibitory for microbial growth and fermentation. Our findings revealed that M. purpureus M630 develops the tolerance and adaptation mechanisms in response to 5'-HMF and Fur during growth and MPs biosynthesis in rice straw hydrolysate. In conclusion, we report that rice straw hydrolysate can serve as an efficient and low-cost substitute for the MP production through submerged fermentation by Monascus spp. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.

语种： 英文

作者： Ma, Yu-Shui;Li, Wen;Liu, Yu;Shi, Yi;Lin, Qin-Lu*;...

期刊： Technology in Cancer Research & Treatment,2020年19:153303381989226 ISSN：1533-0346

通讯作者： Lin, Qin-Lu;Fu, Da

作者机构： [Fu, Da; Ma, Yu-Shui; Li, Wen; Lin, Qin-Lu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Deep Proc, Changsha, Peoples R China.;[Shi, Yi; Liu, Yu; Fu, Da; Ma, Yu-Shui] Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Cent Lab Med Res, Shanghai 200072, Peoples R China.

通讯机构： [Lin, Qin-Lu] C;[Fu, Da] T;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Deep Proc, Changsha, Peoples R China.;Tongji Univ, Sch Med, Shanghai Peoples Hosp 10, Cent Lab Med Res, Shanghai 200072, Peoples R China.

关键词： CRC;CSC;CCSCs;targeted therapy;drug resistance

摘要： As one of the common cancers that threaten human life, the recurrence and metastasis of colorectal cancer seriously affect the prognosis of patients. Although new drugs and comprehensive treatments have been adopted, the current treatment effect on this tumor, especially in advanced colorectal cancer, is still not satisfactory. More and more evidence shows that tumors are likely to be a stem cell disease. In recent years, the rise of cancer stem cell theory has provided a new way for cancer treatment. Studies have found that a small number of special cells in colorectal cancer tissues that induce tumorigenesis, proliferation, and promote tumor migration and metastasis, namely, colorectal cancer stem cells. Colorectal cancer stem cells are defined with a group of cell-surface markers, such as CD44, CD133, CD24, epithelial cell adhesion factor molecule, LGR5, and acetaldehyde dehydrogenase. They are highly tumorigenic, aggressive, and chemoresistant and thus are critical in the metastasis and recurrence of colorectal cancer. Therefore, targeting colorectal cancer stem cells may become an important research direction for the future cure of colorectal cancer.

语种： 英文

作者： Xu, Dong;Lin, Qinlu*;Chang, Huan-Tsung*

期刊： Small Methods,2020年4(4):ARTN 1900387 ISSN：2366-9608

通讯作者： Lin, Qinlu;Chang, Huan-Tsung

作者机构： [Xu, Dong; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China;[Xu, Dong; Lin, Qinlu] Cent South Univ Forestry & Technol, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China;[Chang, Huan-Tsung] Natl Taiwan Univ, Dept Chem, Roosevelt Rd, Taipei 10617, Taiwan;[Chang, Huan-Tsung] Chung Yuan Christian Univ, Dept Chem, Taoyuan 32023, Taiwan

通讯机构： [Lin, Qinlu; Chang, Huan-Tsung] C;[Chang, Huan-Tsung] N;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China. Cent South Univ Forestry & Technol, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China. Natl Taiwan Univ, Dept Chem, Roosevelt Rd, Taipei 10617, Taiwan. Chung Yuan Christian Univ, Dept Chem, Taoyuan 32023, Taiwan.

关键词： carbon dots;fluorescence;imaging;sensing

摘要： Carbon dots (C dots) with biocompatibility, brightness, stability against photoirradiation and salt, and ease in preparation have become important materials for sensing and imaging. They can be prepared from natural materials and small organic molecules through hydrothermal, microwave-assistant, and electrochemical methods, with advantages of simplicity and low cost. To enhance the quantum yields of C dots in the red and near-infrared regions, doping of C dots with heteroatoms such as nitrogen and sulfur has been suggested. C dots both with and without being functionalized recognition elements such as antibodies and aptamers are selective and sensitive for sensing of analytes, including metal ions (e.g., Fe3+, Hg2+, Cu2+), small molecules (e.g., H2O2, cysteine, glutathione), and biopolymers like proteins, as well as for in vitro and in vivo imaging. Depending on the size, charge, and surface ligands of C dots used to label cells, fluorescence images of different organelles are shown. Multicolor images of bacteria, mammalian cells, and plant tissues incubated with C dots are realized when excited at different wavelengths. In this review, many excellent sensing and imaging examples of C dots are presented to highlight their features and to show their challenges for analytical applications.

语种： 英文

作者： Liu, Yuntao*;Tang, Tingting;Duan, Songqi;Li, Cheng;Lin, Qinlu;...

期刊： FOOD & FUNCTION,2020年11(4):3730-3740 ISSN：2042-6496

通讯作者： Liu, Yuntao;Hu, Bin

作者机构： [Duan, Songqi; Hu, Bin; Li, Suqing; Liu, Yuntao; Hu, B; Wu, Hejun; Shen, Li; Liu, Aiping; Tang, Tingting; Li, Cheng; Wu, Dingtao] Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China.;[Liu, Yuntao] Tianjin Univ Commerce, Sch Biotechnol & Food Sci, Tianjin Key Lab Food Biotechnol, Tianjin 300134, Peoples R China.;[Lin, Qinlu; Liu, Yuntao] Cent South Univ Forestry Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Byprod Deep Proc, Changsha 410004, Peoples R China.;[Wu, Wenjuan] Sichuan Agr Univ, Coll Sci, Yaan 625014, Peoples R China.

通讯机构： [Liu, YT; Hu, B] S;[Liu, Yuntao] T;[Liu, Yuntao] C;Sichuan Agr Univ, Coll Food Sci, Yaan 625014, Peoples R China.;Tianjin Univ Commerce, Sch Biotechnol & Food Sci, Tianjin Key Lab Food Biotechnol, Tianjin 300134, Peoples R China.

摘要： Anoectochilus roxburghii, a traditional Chinese medicinal herb, has been widely used for treating numerous chronic diseases. In this study, a polysaccharide from A. Roxburghii (ARPs-p) was purified by anion exchange and size exclusion chromatography. The structural characteristics of ARPs-p were systematically investigated for the first time via numerous chromatographic techniques, periodic acid oxidation, Smith degradation, methylation analysis, FTIR spectroscopy and 1D/2D NMR spectroscopy. The results showed that ARPs-p is a heteropolysaccharide with a molecular weight of 97 kDa; it consists of 97.75% glucose, 1.2% galactose and trace amounts of galacturonic acid, and its backbone structure is composed of →3)-β-d-Glcp-(1→ with some branching points at O-6 linked to non-reducing end units or 6-O-linked Glcp units. Furthermore, streptozotocin (STZ)-induced diabetic mouse experiments suggested that ARPs-p has excellent antihyperglycemic, antioxidant and antihyperlipidemic activities, in which 1,3-β-d-glucan, the main component of ARPs-p, plays a vital role. © The Royal Society of Chemistry.

语种： 英文