摘要:
In this study, we developed a new nanoparticulate system for acetylated starch nanocrystals (ASN) using broken rice. ASN with different degrees of substitution (DS) of 0.04, 0.08 and 0.14 were prepared using acetic anhydride as acetylating agent through reaction with starch nanocrystals (SN). The resulting ASN were investigated for the capability to load and release doxorubicin hydrochloride (DOX), and the antitumor activities of DOX-loaded SN and DOX-loaded ASN were evaluated as potential drug delivery systems for cancer therapy. Cellular uptake and cytotoxicity of nanocrystals and the DOX-loaded nanocrystals were investigated using fluorescence microscopy and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay. Compared with acetylated starches (AS) and native starches (NS), ASN with DS 0.14 loaded up to 6.07% of DOX with a higher loading efficiency of 91.1% and had steadier drug-release rates. Toxicity analysis using the rat hepatocytes model suggested that ASN was biocompatible and could be used for drug delivery. Furthermore, ASN were taken up by cancer cells in vitro and significantly enhanced the cytotoxicity of DOX against HeLa human cervical carcinoma cells. The IC50 value of DOX-loaded ASN-DS 0.14 was 3.8 mu g/mL for 24 h of treatment, which was significantly lower than that of free DOX (21 mu g/mL). These results indicate that the prepared ASN using broken rice is a promising vehicle for the controlled delivery of DOX for cancer therapy. (C) 2016 Elsevier B.V. All rights reserved.
作者机构:
[Yang, Tao; Zhu, Fang; Huang, Huifen; Zhang, Lin; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Yang, Tao; Liu, Junwen] Cent S Univ, Sch Basic Med Sci, Dept Histol & Embryol, Changsha 410013, Hunan, Peoples R China.
通讯机构:
[Liu, Junwen] C;Cent S Univ, Sch Basic Med Sci, Dept Histol & Embryol, Changsha 410013, Hunan, Peoples R China.
关键词:
nanobiosensing;food safety analysis;function of nanomaterials
摘要:
With increasing adulteration, food safety analysis has become an important research field. Nanomaterials-based biosensing holds great potential in designing highly sensitive and selective detection strategies necessary for food safety analysis. This review summarizes various function types of nanomaterials, the methods of functionalization of nanomaterials, and recent (2014–present) progress in the design and development of nanobiosensing for the detection of food contaminants including pathogens, toxins, pesticides, antibiotics, metal contaminants, and other analytes, which are sub-classified according to various recognition methods of each analyte. The existing shortcomings and future perspectives of the rapidly growing field of nanobiosensing addressing food safety issues are also discussed briefly.
摘要:
Development of efficient antibacterial agents is critical for human health. In the present study, we investigated the antibacterial activity of polyethyleneimine (PEI)-capped silver nanoclusters (PEI-AgNCs), based on the fact that nanoclusters normally have higher surface-to-volume ratios than traditional nanomaterials and PEI itself has a strong antimicrobial capacity. We synthesized stable silver nanoclusters by altering PEI molecular weight from 0.6 kDa to 25 kDa and characterized them by UV-Vis absorption and fluorescence spectroscopy and high resolution transmission electron microscopy. The sizes of AgNCs were around 2 nm in diameter and were little influenced by the molecular weight of PEIs. The antibacterial abilities of the four PEI-AgNCs were explored on agar plate and in liquid systems. Our results revealed that the antibacterial activity of PEI-AgNCs is excellent and the reduction of PEI molecular weight could result in the increased antibacterial capacity of PEI-AgNCs. Such proposed new materials might be useful as efficient antibacterial agents in practical clinical applications.
关键词:
Black tea;colonic inflammation;IFN-gamma;IL-1 beta;inflammatory bowel disease;TNF-alpha
摘要:
Inflammatory bowel disease (IBD) is highly prevalent worldwide and includes ulcerative colitis (UC) and Crohn's disease. It is a high incidence rate disease all over the world and an inducement of colon cancer. The aim of this study was to investigate the potential protective effect of Fuzhuan brick tea (FBT) against colitis induced by dextran sulphate sodium (DSS) in mice. ICR mice were administered FBT orally for 7 days before drinking 3% DSS (w/v). The FBT significantly attenuated the symptoms of colitis including diarrhoea, rectal bleeding and loss of body weight. FBT reduced the shortening of colon length and alleviated the histopathological damages. The myeloperoxidase activity, nitric oxide and malondialdehyde level in colon tissues were also significantly decreased by FBT. Besides, FBT treatment obviously suppressed the expression of the inflammatory cytokines such as TNF‐α, IL‐1β and IFN‐γ. Our results provide a safe and efficient method for preventing and treating colitis.
作者机构:
[刘也嘉; 林亲录; 林利忠] College of Food Science and Technology, Central South University of Forestry and Technology, Changsha, China;[刘也嘉; 肖冬梅; 张珺; 林利忠] Jinjian Cereal Industry Co., Ltd, Changde, China
通讯机构:
College of Food Science and Technology, Central South University of Forestry and Technology, Changsha, China
摘要:
为了研究西藏青稞和鸡爪谷的营养成分,水分子存在状态及风味成分,本文采用低场核磁共振(low- field nuclear magnetic resonance,LF-NMR),顶空固相微萃取-气质联用(HS-SPME-GC/MS)方法分析比较了西藏青稞和鸡爪谷的水分子存在状态以及各自挥发性风味组分。结果显示:西藏青稞粗蛋白(24.2%)、膳食纤维(15.20%)和β-葡聚糖(7.61%)含量显著高于鸡爪谷(p<0.05),但鸡爪谷脂肪(1.01%)含量高于青稞。西藏青稞和鸡爪谷中结合水含量分别占88.4%和87.7%。青稞和鸡爪谷中分别检测出72和70种挥发性成分,青稞挥发性风味组分主要包括烃类(44.37%)、醇类(18.06%)和醛类(14.03%),鸡爪谷香气主要组分为醇类(34.81%)、烃类(34.65%)和酮类(9.78%)。总体分析,青稞比鸡爪谷的营养成分更加丰富,结合水含量比例更高,风味物质更加突出,为西藏青稞和鸡爪谷的加工及合理膳食提供参考。