Anti-hyperlipidemic effect of rice bran polysaccharide and its potential mechanism in high-fat diet mice
作者：
Nie, Ying;Luo, Feijun* ;Wang, Long;Yang, Tao;Shi, Limin;...
期刊：
FOOD & FUNCTION ,2017年8(11):4028-4041 ISSN：2042-6496
通讯作者：
Luo, Feijun;Lin, Qinlu
作者机构：
[Wang, Long; Luo, Feijun; Yang, Tao; Guo, Ting; Shi, Limin; Luo, FJ; Lin, Qinlu; Shen, Junjun; Nie, Ying; Xu, Wei] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Coll Food Sci & Engn, Lab Mol Nutr, Changsha 410004, Hunan, Peoples R China.;[Nie, Ying] Hunan Biol & Electromech Polytech, Changsha 410128, Hunan, Peoples R China.;[Li, Xinhua] Cent S Univ, Xiangya Hosp, Dept Gastroenterol, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Luo, FJ; Lin, QL] C;Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Coll Food Sci & Engn, Lab Mol Nutr, Changsha 410004, Hunan, Peoples R China.
摘要：
Hyperlipidemia occurs very often in modern society along with a high calorie intake and is regarded as one of the greatest risk factors for the prevalence of cardiac vascular disease (CVD). In this study, we investigated the anti-hyperlipidemic effect of the rice bran polysaccharides (RBP) and its mechanism in a high fat diet animal model. 60 ICR mice were randomly divided into 3 groups, which included Control, HFD (high fat diet) and HFD + RBP, and each group included 20 mice. The control group was fed with a standard diet while the other two groups were fed with HFD. In addition, the HFD + RBP group was fed with 500 mg kg-1 of rice bran polysaccharides by intragastric administration while the other two groups were intragastrically administered with water. The results showed that RBP treatment for 10 weeks obviously decreased the body weight, liver weight and adipose tissues of mice; and it decreased the levels of total cholesterol (TC), triglycerides (TG) and low density lipoprotein-cholesterol (LDL-c) in the plasma. H&E staining of the liver tissues showed that RBP treatment decreased the size of fat droplets compared with the HFD group. Microarray analysis revealed that RBP treatment results in 80 genes being up-regulated while 72 genes were down-regulated in the tissues of liver. IPA software analysis suggested that NF-κB may play a vital role in the lipid-lowering effect of RBP. Real-time quantitative PCR confirmed that the mRNA levels of PPAR-α, PPAR-γ, PPAR-δ, SREBP-1C, FASN, ACC, SIRT and CD36, which are related to lipid metabolism, were significantly regulated by RBP supplementation compared to HFD. The western blot analysis further confirmed these altered expressions after RBP treatment. Taken together, these results suggest that the oral administration of RBP exerts lipid-lowering in high fat diet mice via regulating the lipid metabolism-related gene expression. © 2017 The Royal Society of Chemistry.
语种：
英文
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以培养目标达成度为导向的食品营养学课程设计
作者：
曹清明;周文化;林亲录;龚吉军
期刊：
湖南科技学院学报 ,2017年38(7):139-141 ISSN：1673-2219
作者机构：
稻谷及副产物深加工国家工程实验室/中南林业科技大学 食品科学与工程学院,湖南 长沙,410004
关键词：
工程教育认证;毕业要求;指标点;培着目标;达成度;食品营养学
摘要：
论文围绕工程教育认证的需要,进行了基于培养目标达成度的食品营养学课程教设计。在分析专业毕业要求的基础上确定了食品营养学课程毕业要求指标点;研究了以培养目标达成度为导向的食品营养学课程教学目标、教学方法和考核评价方法,以便有效的提高食品营养学的教学质量。
语种：
中文
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ASLT法研究复合精油微胶囊对大米保鲜效果
作者：
邓靖;李文;林亲录;张静;熊婷;...
期刊：
现代食品科技 ,2017年33(3):196-202 ISSN：1673-9078
通讯作者：
Lin, Q.-L.
作者机构：
稻谷及副产物深加工国家工程实验室,中南林业科技大学,湖南长沙410004;湖南工业大学包装与材料工程学院,湖南株洲412007;湖南工业大学包装与材料工程学院,湖南株洲,412007
通讯机构：
National Engineering Laboratory for Rice and By-product Deep Processing, Center South University of Forestry & Technology, Changsha, China
关键词：
大米;复合精油微胶囊;加速货架期试验;脂肪酸值
摘要：
采用共沉淀法制备壁材为β-环糊精,芯材为对大米病原菌黄曲霉有高抑菌性的大蒜精油与肉桂精油复合精油的微胶囊保鲜剂;将微胶囊用无纺布包装成小袋置于大米PE包装袋中,采用加速货架期试验,根据大米脂肪酸值的变化评价其保鲜效果,并预测大米货架寿命;同时分析大米感官评分与脂肪酸值的相关性,为快速判断大米质量提供参考。结果表明:大蒜精油和肉桂精油对黄曲霉抑制效果显著,复合精油以大蒜精油:肉桂精油为1:3组成;SEM表征分析微胶囊呈不规则形状,复合精油微胶囊处理的大米脂肪酸值的变化速度明显低于对照组,预测复合精油微胶囊处理的大米在室温（25℃）的货架寿命为415 d,而对照组大米货架寿命仅为122 d;大米感官评分与脂肪酸值变化具有一定的相关性。
语种：
中文
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An ESIPT-based two-photon fluorescent probe detection of hydrogen peroxide in live cells and tissues
作者：
Zhou, Liyi* ;Peng, Yongbo;Wang, Qianqian;Lin, Qinlu
期刊：
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY ,2017年167:264-268 ISSN：1011-1344
通讯作者：
Zhou, Liyi
作者机构：
[Zhou, Liyi; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Zhou, Liyi] Hunan Univ Technol, Coll Life Sci & Chem, Zhuzhou 412007, Hunan, Peoples R China.;[Wang, Qianqian; Peng, Yongbo] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Mol Sci & Biomed Lab, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Zhou, Liyi] C;Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
Hydrogen peroxide;Boric acid ester;Solid-state lighting;Two-photon
摘要：
A variety of diseases associated with human aging, which have a strong oxidative stress, but connecting age-related diseases and oxidative stress of the basic molecular mechanisms still insufficiently understood. Oxidative stress origins from the unregulated production of reactive oxygen species (ROS), and oxidative damaging to tissues and organs from subsequent oxidation-reduction chemistry by cellular mismanagement. In particular, H2O2 is a major by-product of ROS in live organisms and a common marker for oxidative stress, and its dynamic equilibrium can have various physiological and pathological consequences. H2O2 is a small molecule, but it is an essential oxygen metabolite in living systems and acts as an important compound in cellular signal transduction by reversible oxidation of proteins. To quantitatively detect of H2O2 in biosystems, herein, we adopted a 2-(2'-hydroxyphenyl)-4(3H)-quinazolinone (HPQ), a small organic fluorophore known for its luminescence mechanism through excited-state intramolecular proton transfer (ESIPT). HPQ was employed as a precursor to develop a turn-on probe (HPQ-H) for bioimaging applications. After cleavaging the boronic ester moiety by H2O2, HPQ-H releases a HPQ fiuorophore which shows a 45-fold fluorescence intensity enhancement with high sensitivity and selectivity over other reactive oxygen species (ROS), and a high resolution imaging and large tissue-imaging depth (70-170 pm) in living cells and tissues images under two-photon excitation (720 nm). (C) 2017 Elsevier B.V. All rights reserved.
语种：
英文
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鱼糜中活泼羰基化合物在胶凝过程中的变化
作者：
付湘晋;刘颖;李忠海;林亲录
期刊：
食品工业科技 ,2017年38(10):72-75 ISSN：1002-0306
作者机构：
中南林业科技大学食品科学与工程学院, 湖南, 长沙, 410004;长沙环境保护职业技术学院环境科学系, 湖南, 长沙, 410004;北卡州立大学食品生物工程与营养科学系, 美国, 罗利, 27695;[付湘晋; 李忠海; 林亲录] 中南林业科技大学食品科学与工程学院, 湖南, 长沙, 410004;[刘颖] 长沙环境保护职业技术学院环境科学系, 湖南, 长沙, 410004
关键词：
鱼糜;凝胶;活泼羰基化合物;丙二醛;4-羟基-己烯醛;4-羟基-壬烯醛
摘要：
本文采用五氟苯肼原位衍生化-固相微萃取-气相/质谱法检测了鲢鱼鱼糜、冷冻鱼糜及鱼糜凝胶中的三种活泼羰基化合物(RCCs):丙二醛(MDA)、4-羟基-己烯醛(HHE)、4-羟基-壬烯醛(HNE);并采用添加五氟苯肼清除RCCs,阻断RCCs-蛋白质反应,分析鱼糜中RCCs对鱼糜蛋白胶凝特性的影响。结果表明:新鲜鲢鱼糜中MDA(0.42 ± 0.02) mg/kg、HHE(0.21 ±0.03) mg/kg含量高于HNE(0.07 ± 0.00) mg/kg;冷冻鱼糜RCCs含量高于新鲜鱼糜,MDA、 HHE、HNE含量分别为(1.14 ±0.03)、(0.86 ±0.03)、(0.18 ±0.02) mg/kg。新鲜鱼糜加热胶凝后,各RCC含量均显著上升(p < 0.05),冷冻鱼糜加热胶凝后,各RCC含量均显著(p< 0.05)下降;添加RCCs清除剂阻断RCCs-蛋白质反应后,冷冻鱼糜凝胶(40 ℃1 h-90 ℃30 min)强度从(243.52 ±25.04) g × cm下降到(190.53 ±20.33) g × cm(p <0.05);所以,RCCs加强了冷冻鱼糜凝胶的强度。RCCs对鱼糜凝胶的保水性无明显影响。
语种：
中文
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Soy protein-derived ACE-inhibitory peptide LSW (Leu-Ser-Trp) shows anti-inflammatory activity on vascular smooth muscle cells
作者：
Lin, Qinlu;Liao, Wang;Bai, Jie;Wu, Wei;Wu, Jianping*
期刊：
Journal of Functional Foods ,2017年34:248-253 ISSN：1756-4646
通讯作者：
Wu, Jianping
作者机构：
[Wu, Wei; Bai, Jie; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Engn, Changsha 410004, Hunan, Peoples R China.;[Wu, Jianping; Liao, Wang] Univ Alberta, Dept Agr Food & Nutr Sci, 4-10 Ag For Ctr, Edmonton, AB T6G 2P5, Canada.
通讯机构：
[Wu, Jianping] U;Univ Alberta, Dept Agr Food & Nutr Sci, 4-10 Ag For Ctr, Edmonton, AB T6G 2P5, Canada.
关键词：
ACE-inhibitory peptide;Inflammation;LSW;Soy protein;VSMC
摘要：
Soy proteins are a rich source of various bioactive peptides. LSW (Leu-Ser-Trp) was previously identified from thermolysin-digested soy protein hydrolysate as a potent ACE-inhibitory peptide; however, its biological effects on vascular cells have not been elucidated. The present study evaluated anti-oxidant and anti-inflammatory activities of LSW on vascular smooth muscle cells (VSMCs). Ang II promoted oxidative stress and inflammation in VSMCs; adding LSW did not show anti-oxidant activity, while COX-2, but not iNOS, was down-regulated in Ang II-stimulated VSMCs, suggesting its anti-inflammatory activity. AT1R mediates most of the pathological effects of Ang II in VSMCs; LSW down-regulated Ang II-stimulated AT1R expression, via decreased phosphorylation of both Src and ERK1/2. Furthermore, LSW could also reduce the phosphorylation of nuclear transcription factor p50, but not p65. Results of this study suggested a novel function of LSW as an anti-inflammatory agent on VSMCs, which might broaden its uses as functional food ingredients.
语种：
英文
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Label-free, water-soluble fluorescent peptide probe for a sensitive and selective determination of copper ions
作者：
Yang, Tao;Zhu, Fang;Zhou, Tianxiao;Cao, Jianzhong;Xie, Yibo;...
期刊：
Analytical Sciences ,2017年33(2):191-196 ISSN：0910-6340
通讯作者：
Zhang, Lin
作者机构：
[Zhou, Tianxiao; Yang, Tao; Zhu, Fang; Cao, Jianzhong; Zhang, Meiyi; Xie, Yibo; Zhang, Lin; Lin, Qinlu; Wang, Yan] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China.;[Zhou, Tianxiao; Yang, Tao; Zhu, Fang; Cao, Jianzhong; Zhang, Meiyi; Xie, Yibo; Zhang, Lin; Lin, Qinlu; Wang, Yan] Cent South Univ Forestry & Technol, Dept Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Cao, Dong-Sheng] Cent S Univ, Sch Pharmaceut Sci, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Dept Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
Copper ion;determination;fluorescent peptide probe;selectivity;water sample
摘要：
This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu 2+ ) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu 2+ via quenching of the probe fluorescence upon a binding of Cu 2+ . The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu 2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu 2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu 2+ in aqueous solutions. © 2017 The Japan Society for Analytical Chemistry.
语种：
英文
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面向大学生的实验开放新模式
作者：
王素燕;林亲录;许东;肖华西
期刊：
高校实验室科学技术 ,2017年(03):76-78
作者机构：
中南林业科技大学稻谷及副产物深加工国家工程实验室/食品科学与工程学院
关键词：
实验开放模式;创新能力;实践能力;大学生
摘要：
以培养大学生创新能力和实践能力为目标,结合食品科学与工程学院的工作实践,建立了大学生实验＂五开放＂模式,包括实验平台、实验团队组合、实验时间、实验内容和实验指导方式5个方面。＂五开放＂模式能够有效地培养学生创新及实践能力,为培养创新实践型人才奠定良好的基础。
语种：
中文
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Effects of Non-Starch Polysaccharides on Inflammatory Bowel Disease
作者：
Nie, Ying;Lin, Qinlu;Luo, Feijun*
期刊：
International Journal of Molecular Sciences ,2017年18(7) ISSN：1661-6596
通讯作者：
Luo, Feijun
作者机构：
[Luo, Feijun; Nie, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Lab Mol Nutr, Natl Engn Lab Deep Proc Rice & Byprod, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Nie, Ying] Coll Hunan Biol & Electromech Polytech, Dept Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.
通讯机构：
[Luo, Feijun] C;Cent South Univ Forestry & Technol, Lab Mol Nutr, Natl Engn Lab Deep Proc Rice & Byprod, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
non-starch polysaccharide (NSP);inflammatory bowel disease (IBD);intervention;mechanism;SCFAs;gut microbiota;immune system;pro-inflammatory cytokines
摘要：
The incidence of inflammatory bowel disease (IBD) has increased considerably over the past few decades. In the present review, we discuss several disadvantages existing in the treatment of IBD and current understandings of the structures, sources, and natures of various kinds of non-starch polysaccharides (NSPs). Available evidences for the use of different sources of NSPs in IBD treatment both in vitro and in vivo are analyzed, including glucan from oat bran, mushroom, seaweed, pectin, gum, prebiotics, etc. Their potential mechanisms, especially their related molecular mechanism of protective action in the treatment and prevention of IBD, are also summarized, covering the anti-inflammation, immune-stimulating, and gut microbiota-modulating activities, as well as short-chain fatty acids (SCFAs) production, anti-oxidative stress accompanied with inflammation, the promotion of gastric epithelial cell proliferation and tissue healing, and the reduction of the absorption of toxins of NSPs, thus ameliorating the symptoms and reducing the reoccurrence rate of IBD. In summary, NSPs exhibit the potential to be promising agents for an adjuvant therapy and for the prevention of IBD. Further investigating of the crosstalk between immune cells, epithelial cells, and gut microorganisms in addition to evaluating the effects of different kinds and different molecular weights of NSPs will lead to well-designed clinical intervention trials and eventually improve the treatment and prevention of IBD.
语种：
英文
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药用植物对肿瘤细胞分化诱导作用研究进展
作者：
梁盈;黄萍;李佳佳;王荣;马酉初;...
期刊：
食品与生物技术学报 ,2017年36(8):785-791 ISSN：1673-1689
作者机构：
[梁盈; 黄萍; 李佳佳; 王荣; 马酉初; 林亲录] 中南林业科技大学, 稻谷及副产物深加工国家工程实验室, 湖南, 长沙, 410004
关键词：
诱导分化;药用植物;肿瘤细胞
摘要：
肿瘤的诱导分化研究一直是肿瘤治疗研究领域行之有效的手段之一。自采用全反式维甲酸进行诱导分化,对急性早幼粒细胞白血病治疗取得了较好的疗效后,诱导分化就吸引了越来越多学者的注意。药用类植物不仅具有药用价值,还具有一定的食用价值和经济价值。目前越来越重视药用类植物的开发利用,药用植物来源的分化诱导剂因其副作用小、毒性低等优点被较广泛地应用于治疗肿瘤细胞研究中。本文综述了药用植物源活性成分在肿瘤细胞诱导分化治疗研究领域的现状及其相关作用机制。
语种：
中文
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米糠贮藏期间米糠谷蛋白结构变化的研究
作者：
吴伟;吴晓娟;蔡勇建;梁盈;林亲录
期刊：
中国粮油学报 ,2017年32(7):98-102,120 ISSN：1003-0174
作者机构：
[吴伟; 吴晓娟; 蔡勇建; 梁盈; 林亲录] 中南林业科技大学食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 长沙, 410004
关键词：
米糠;贮藏;米糠谷蛋白;蛋白质氧化;聚集
摘要：
将新鲜米糠贮藏不同时间后脱脂制备米糠谷蛋白,研究米糠酸败对米糠谷蛋白结构的影响。结果表明,随着新鲜米糠贮藏时间的延长,米糠谷蛋白羰基、二硫键含量增加,游离巯基含量下降,表明米糠酸败导致米糠谷蛋白氧化。随着新鲜米糠贮藏时间的延长,米糠谷蛋白二级结构中α-螺旋和β-折叠含量下降,β-转角和无规卷曲含量上升,Zeta电位绝对值、表面疏水性和内源荧光强度下降,内源荧光最大吸收波长发生蓝移,并且伴随着蛋白质聚集体的出现,表明米糠酸败使得米糠谷蛋白结构发生变化,逐渐形成氧化聚集体。
语种：
中文
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食品营养学课程达成度分析
作者：
曹清明;林亲录;周文化
期刊：
长沙大学学报 ,2017年31(2):134-137 ISSN：1008-4681
作者机构：
稻谷及副产物深加工国家工程实验室,湖南 长沙 410004;中南林业科技大学食品科学与工程学院,湖南 长沙 410004;[周文化; 曹清明; 林亲录] 中南林业科技大学
关键词：
食品营养学;毕业要求;指标点;课程达成度
摘要：
围绕工程教育认证的需要,阐述了食品营养学课程毕业要求的指标点.对照毕业要求指标点,对食品营养学的理论教学、实验教学和实习教学进行了达成度分析,并通过问卷调查对学生学习效果进行了进一步的达成度分析.分析表明,学生对于计算机以及专业知识的运用能力不够.因此,建议在2016版新的培养方案中已增加8课时计算机上机课的前提下,针对难点和重点内容,深化研讨式教学,以加深学生对知识的理解和运用和保证教学的持续改进.并指出了继续完善课程目标达成度分析的几个努力方向.
语种：
中文
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米糠贮藏时间对米糠蛋白体外胃蛋白酶消化性质及其消化产物抗氧化性的影响
作者：
何莉媛;吴伟;吴晓娟;付旭恒;林亲录
期刊：
中国油脂 ,2017年42(11):70-74 ISSN：1003-7969
作者机构：
[何莉媛; 吴伟; 吴晓娟; 付旭恒; 林亲录] 中南林业科技大学食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 长沙, 410004
关键词：
米糠贮藏;米糠蛋白;蛋白质氧化;体外消化;抗氧化性
摘要：
新鲜米糠贮藏不同时间后制备米糠蛋白,研究米糠贮藏时间对米糠蛋白体外胃蛋白酶消化性质及其消化产物抗氧化性的影响。结果表明:随着米糠贮藏时间的延长,米糠蛋白体外胃蛋白酶消化率和初始消化速率先增加后下降,在米糠贮藏3 d时达到最大值,分别为39.73%和0.381 h~(-1) ;随着米糠贮藏时间的延长,米糠蛋白体外胃蛋白酶消化产物清除ABTS~+·、DPPH·、·OH和O_2~- ·的能力以及金属螯合能力、还原能力先增加后下降。研究表明,新鲜米糠短期贮藏(3 d)会促进米糠蛋白在胃蛋白酶中的消化,提高消化产物的抗氧化性;米糠长期贮藏抑制米糠蛋白在胃蛋白酶中的消化,降低体外消化产物的抗氧化性。
语种：
中文
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思维导图教学策略在高校现代食品检测技术教学中应用
作者：
许东;王素燕;林亲录
期刊：
智库时代 ,2017年(10):143+145 ISSN：2096-4609
作者机构：
中南林业科技大学食品科学与工程学院
关键词：
思维导图;现代食品检测技术;教学改革
摘要：
现代食品检测技术是高校食品质量与安全专业里重要的一门专业课。由于涉及学科多,教学难度大,课程教学效果不理想。将思维导图策略引入到教学中,能够帮助学生整理总结课程知识点,形成知识体系,促使学生全面掌握现代食品检测技术知识,培养学生分析和解决问题的能力。
语种：
中文
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Molecular engineering of d-A-d-based non-linearity fluorescent probe for quick detection of thiophenol in living cells and tissues
作者：
Zhou, Liyi* ;Lin, Qinlu;Liu, Sihua;Tan, Yi;Sun, Hongyan
期刊：
Sensors and Actuators B-Chemical ,2017年244:958-964 ISSN：0925-4005
通讯作者：
Zhou, Liyi
作者机构：
[Zhou, Liyi; Lin, Qinlu] Cent South Univ Forestry & Technol Changsha, Coll Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Zhou, Liyi; Liu, Sihua] Hunan Univ Technol, Coll Life Sci & Chem, Zhuzhou 412007, Hunan, Peoples R China.;[Tan, Yi; Sun, Hongyan] City Univ Hong Kong, Dept Biol & Chem, 83 Tat Chee Ave, Kowloon, Hong Kong, Peoples R China.;[Zhou, Liyi] Cent South Univ Forestry & Technol, Changsha, Hunan, Peoples R China.
通讯机构：
[Zhou, Liyi] C;Cent South Univ Forestry & Technol, Changsha, Hunan, Peoples R China.
关键词：
Fluorescent imaging;Thiophenols;Tissues;Two-photon
摘要：
Thiophenols are widely utilized for producing pesticides, polymers and pharmaceuticals. Studies have shown that prolonged exposure of human to thiolphenol can result in serious health problems such as significant damage of the central nervous system. Herein, we have developed a novel turn-on two-photon fluorescent probe TP-ASH for selective and sensitive detection of thiophenols. We first constructed a new two-photon dye by connecting a coumarin fluorophore and a naphthalene fluorophore through 2,5-disubstituted-1,3,4-oxadiazole ring to afford D-pi-A-pi-D-structure. Fluorescent studies showed that this new dye possess large two -photon active absorption across-section (250 GM) and high fluorescence quantum yield (0.85). Encouraged by the results, we proceeded to conjugate this novel dye to a thiophenol recognition moiety, 4-dinitrobenzene-ether (DNB). The resulting probe displayed high selectivity towards thiophenols over their similar analogues, aliphatic thiols. The probe also exhibited high sensitivity, with a detection limit of 6.7 nM and a fluorescence enhancement of 163-fold. In addition, we have successfully applied the probe for two-photon imaging studies of thiphenols in living cells and mice tissues. We envision that the probe will add useful tools in detecting thiophenols in environmental samples and biological systems. (C) 2017 Elsevier B.V. All rights reserved.
语种：
英文
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稻谷老化机制研究进展
作者：
赵娟红;林亲录;孙术国;王若晖;张素红;...
期刊：
食品工业科技 ,2017年38(23):326-329 ISSN：1002-0306
作者机构：
[赵娟红; 林亲录; 孙术国; 王若晖; 张素红; 丁玉琴; 曹建中] 中南林业科技大学食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 湖南, 长沙, 410004
关键词：
稻谷;陈化;机理
摘要：
稻米老化是一个复杂的过程。虽然淀粉、细胞壁残留物、蛋白质和脂质的总含量没有显著变化,但老化过程却引起了许多化学基团组分的改变以及大分子之间的相互作用。本文主要综述了稻米在储藏过程中的品质变化及机制,展望了我国稻米储藏研究的未来发展方向,为我国稻谷的安全储藏提供参考。
语种：
中文
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发芽糙米重组米制备方便米饭的研究
作者：
吴伟;吴晓娟;从竞远;林亲录
期刊：
中国粮油学报 ,2017年32(9):1-7 ISSN：1003-0174
作者机构：
[吴伟; 吴晓娟; 从竞远; 林亲录] 中南林业科技大学食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 长沙, 410004
关键词：
发芽糙米;双螺杆挤压;发芽糙米重组米;方便米饭
摘要：
研究双螺杆挤压对发芽糙米重组米复水性能、营养成分及糊化特性的影响,随后以发芽糙米重组米为原料制备方便米饭,研究蒸煮和干燥工序对发芽糙米重组米方便米饭食用品质的影响。结果表明:螺杆转速120 r/min,三、四区挤压温度120 ℃、物料含水量20%时,发芽糙米重组米复水率最高,且糊化特性优于发芽糙米;相比于发芽糙米,发芽糙米重组米总淀粉、直链淀粉和可溶性蛋白含量下降,总蛋白、粗脂肪、纤维素和氨基丁酸含量几乎不变。米水比1 : 1.3、蒸煮30 min时,方便米饭的感官评分最高,且硬度和黏着性适中;蒸煮方便米饭经560 W-60 ℃微波热风组合干燥速度最快,此时得到的干燥方便米饭复水时间最短,复水率和碘蓝值最高。发芽糙米和发芽糙米重组米制备的方便米饭色泽和香味都较好,但发芽糙米重组米方便米饭的形态、口感和滋味更好。
语种：
中文
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海藻酸钠与钙离子对大米淀粉糊化的影响
作者：
杨英;何璐;向忠琪;周艳青;林亲录
期刊：
中国粮油学报 ,2017年32(2):37-42 ISSN：1003-0174
作者机构：
[杨英; 何璐; 向忠琪; 周艳青; 林亲录] 中南林业科技大学食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 长沙, 410004
关键词：
大米淀粉;糊化;海藻酸钠;钙离子;凝胶
摘要：
为了探究海藻酸钠与钙离子对大米淀粉糊化的影响,分别采用快速黏度分析仪和流变仪测定了大米淀粉在海藻酸钠和钙离子存在下的成糊特性与淀粉糊状态,并用扫描电子显微镜观察了大米淀粉的糊化显微结构。结果表明,钙离子对大米淀粉的成糊特性无显著性影响;海藻酸钠将体系黏度提高了135%,并使大米淀粉糊呈现似液状态(tanδ<1);在体系水分蒸发脱离的条件下,海藻酸钠与钙离子形成了网状凝胶结构,并使大米淀粉糊呈现稳定的似固状态(tanδ>1)。因此,海藻酸钠与钙离子能够在大米淀粉糊化过程中形成浓缩诱导型胶凝,从而使大米淀粉糊的结构状态相对固定化。
语种：
中文
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Octacosanol Attenuates Inflammation in Both RAW264.7 Macrophages and a Mouse Model of Colitis
作者：
Guo, Tianyi;Lin, Qinlu;Li, Xinhua;Nie, Ying;Wang, Long;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(18):3647-3658 ISSN：0021-8561
通讯作者：
Luo, Feijun
作者机构：
[Wang, Long; Hu, Tao; Luo, Feijun; Guo, Ting; Guo, Tianyi; Shi, Limin; Nie, Ying; Xu, Wei; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Byprod, Lab Mol Nutr, Changsha 410004, Hunan, Peoples R China.;[Li, Xinhua] Cent S Univ, Xiangya Hosp, Dept Gastroenterol, Changsha 410008, Hunan, Peoples R China.;[Luo, Feijun] Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Lab Mol Nutr, 498 Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Luo, Feijun] C;Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Lab Mol Nutr, 498 Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.
关键词：
octacosanol;colitis;inflammation factor;MAPK;NF-kappa B;AP-1
摘要：
Octacosanol has multiple biological functions. In this study, the anti-inflammatory effect and molecular mechanism of octacosanol were evaluated by using dextran sulfate sodium (DSS)-induced ulcerative colitis model in mice and lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells. The colitis mouse model was induced by 3.0% DSS in 8-week ICR mice and octacosanol orally administered with 100 mg/kg/day. The results showed that octacosanol significantly improved the health status of mice and reduced DSS-induced pathological damage in the colonic tissues. Octacosanol obviously inhibited the mRNA and protein expression levels of pro-inflammatory factors of colonic tissues. In vitro, octacosanol administration significantly reduced the expression of mRNA or protein of pro-inflammatory cytokines and the phosphorylation of c-Jun N-terminal kinase and p38, and it also partly prevented LPS-induced translocations of NF-κB and AP-1. Octacosanol has anti-inflammatory effect, and its molecular mechanism may be involved in downregulating the expression of inflammatory factors and blocking of MAPK/NF-κB/AP-1 signaling pathway. ©2017 American Chemical Society.
语种：
英文
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Oryzanol Modifies High Fat Diet-Induced Obesity, Liver Gene Expression Profile, and Inflammation Response in Mice
作者：
Wang, Long;Lin, Qiniu* ;Yang, Tao;Liang, Ying;Nie, Ying;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(38):8374-8385 ISSN：0021-8561
通讯作者：
Lin, Qiniu;Luo, Feijun
作者机构：
[Wang, Long; Lin, QN; Luo, FJ; Tang, Yiping; Luo, Feijun; Yang, Tao; Lin, Qiniu; Fu, Xiangjin; Shen, Junjun; Nie, Ying; Liang, Ying] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Key Lab Hunan Prov Special Med Food, Lab Mol Nutr,Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Luo, Yi] Cent S Univ, Xiangya Sch Med, Dept Clin Med, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Lin, QN; Luo, FJ] C;Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Key Lab Hunan Prov Special Med Food, Lab Mol Nutr,Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
NF-kappaB;gene chip;hyperlipidemia;lipid metabolism;oryzanol
摘要：
In Western countries and China, the dietary habit of high calories usually results in hyperlipidemia, which is closely associated with cardiovascular diseases. In the study, we investigated the antihyperlipidemic effect of oryzanol and its molecular mechanism in the high fat diet (HFD) mouse model. In total, 60 ICR mice were randomly divided into control group, HFD group, and HFD+Ory group. The mice from the HFD+Ory group were additionally fed with 100 mg/kg of oryzanol by intragastric administration. Our data indicated that oryzanol treatment for 10 weeks significantly reduced bodyweight, liver weight, and adipose tissues weight of the mice;lowered the contents of total cholesterol (TC), triglycerides (TG), and low density lipoprotein-cholesterol (LDL-C);and elevated high density lipoprotein-cholesterol (HDL-C) in the plasma of HFD mice. Compared with the HFD group, H&E staining showed that oryzanol treatment decreased the size of fat droplets of liver tissues and the size of adipocytes. Gene chip data found that oryzanol administration caused 32 genes to increase expressions while 60 genes had reduced expressions in the liver tissues of HFD mice. IPA software was used to analyze the protein interaction network and found that transcript factor NF-κB located in the central role of network, meaning NF-κB may have important function in the lipid-lowering effect of oryzanol. Western blotting and RT-qPCR confirmed that lipid metabolism-related gene expressions were obviously regulated by oryzanol administration. Oryzanol also inhibited expressions of inflammatory factor in the liver tissues of HDF mice. Taken together, our data indicate that oryzanol treatment can regulate lipid metabolism-related gene expressions and inhibit HDF-caused obesity in mice. ©2017 American Chemical Society.
语种：
英文
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