摘要：
To address conventional powder catalysts' recovery and aggregation issues that greatly restrain their practical application, a recoverable graphene oxide (GO)-supported 3D-MoS(2)/FeCo(2)O(4) sponge (SFCMG) was developed through a simple impregnation pyrolysis method. SFCMG can efficiently activate peroxymonosulfate (PMS) to produce reactive species for rapid degradation of rhodamine B (RhB), with 95.0% and 100% of RhB being removed within 2min and 10min, respectively. The presence of GO enhances the electron transfer performance of the sponge, and the three-dimensional melamine sponge serves as a substrate to provide a highly dispersed carrier for FeCo(2)O(4) and MoS(2)/GO hybrid sheets. SFCMG exhibits a synergistic catalytic effect of Fe and Co, and facilitates the redox cycles of Fe(III)/Fe(II) and Co(III)/Co(II) by MoS(2) co-catalysis, which enhances its catalytic activity. Electron paramagnetic resonance results demonstrate that SO(4)(•-), ·O(2)(-) and (1)O(2) are all involved in SFCMG/PMS system, and (1)O(2) played a prominent role in RhB degradation. The system has good resistance to anions (Cl(-), SO(4)(2-), and H(2)PO(4)(-)) and humic acid and excellent performance for many typical contaminants degradation. Additionally, it works efficiently over a wide pH range (3-9) and possesses high stability and reusability with the metal leaching far below the safety standards. The present study extends the practical application of metal co-catalysis and offers a promising Fenton-like catalyst for the treatment of organic wastewater.
摘要：
As a major component of lignocellulosic biomass, lignin has lots of fascinating properties, which make them become interesting natural polymer raw material. Here, the lignin isolation, modification strategies, polymerization method and process, and the promising applications were summarized and analyzed in recent years. Specially, the preparation of high excellent adsorbents from deconstructed lignin products are highlighted from the synthesis methods and adsorption mechanism perspective. Abstract As a major component of lignocellulosic biomass, lignin has lots of fascinating properties, such as regeneration, biocompatibility, antioxidant, hydrophilicity, unique aromatic structure, and various functional groups, which make them become interesting natural polymer raw material, and attracted more and more attention. In particular, the rich functional groups of lignin can be easily functionalized through various synthesis methods, which can endow lignin‐based polymers with new structure and function. These functional lignin‐based polymers are widely used in environmental remediation, smart hydrogel materials, catalysis, biomedical, etc. However, its various sources and types, the complex structure, relatively weak chemical reactivity, the unstable processing property, and few high value‐added products limited their practical applications. With the development of lignin varieties, modified reagent, synthesis methods, and polymerization process, many works had been published for the synthesis and application of lignin‐based polymers. In this review, the lignin isolation, modification strategies, polymerization method and process, and the promising applications were summarized and analyzed in recent years. Specially, the recent advances in the preparation of high excellent adsorbents from deconstructed lignin products are highlighted from the synthesis methods and adsorption mechanism perspective. Finally, we discussed these challenges in the green and controllable synthesis of lignin‐based polymers, and prospected their functional applications.
通讯机构：
[Liu, GQ ] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.
摘要：
Ganoderma is a prize medicinal macrofunguswitha broad range of pharmaceutical values. To date, various attemptshave been made to cultivate Ganoderma to improvethe production of secondary metabolites with pharmacological activity.Among the adopted techniques, protoplast preparation and regenerationare indispensable. However, the evaluation of protoplasts and regeneratedcell walls usually relies on electron microscopy assays, which requiretime-consuming and destructive sample preparation and merely providelocalized information in the selected area. In contrast, fluorescenceassays enable sensitive real-time detection and imaging invivo. They can also be applied to flow cytometry, providinga collective overview of every cell in a sample. However, for macrofungisuch as Ganoderma, the fluorescence analysis of protoplastsand regenerated cell walls is difficult owing to the hindrance ofthe homologous fluorescent protein expression and the lack of an appropriatefluorescence marker. Herein, a specific plasma membrane probe, TAMRAperfluorocarbon nucleic acid probe (TPFN), is proposed for the nondestructiveand quantitative fluorescence analysis of cell wall regeneration.Exploiting the perfluorocarbon membrane-anchoring chains, hydrophilicnucleic acid linker, and fluorescent dye TAMRA, the probe is provento be selective, soluble, and stable, enabling rapid fluorescencedetection of a protoplast sample free of transgenic expression orimmune staining. Based on the TPFN and flow cytometry techniques,a quantitative approach is constructed to monitor the process of cellwall growth in a fast, quantitative, and high-throughout manner, andthe obtained results are consistent with those of conventional electronmicroscopy. In principle, with slight modifications or integration,the proposed probe and approach can be adapted to the preparationof cell protoplasts, inspection of cell wall integrity under environmentalstress, and programmable membrane engineering for cytobiology andphysiology research.
摘要：
An electrochemical aptasensor was established for the fast and sensitive determination of tetracycline based on the synergistic effect of MoS2-thionine nanocomposite and aptamer. The glassy carbon electrode was coated with MoS2 nanosheets of thionine to immobilize the tetracycline aptamer. In the absence of tetracycline, large quantities of thionin were loaded on the surface of the MoS2 nanosheet to serve as the electrochemical probe. After tetracycline was added to the surface of the electrode, an aptamer analyte complex was produced on the modified electrode. Through intercalation and electrostatic interactions, the complex was substantially adsorbed onto thionine, and the peak currents of the redox signal of thionine decreased. Therefore, the concentration of tetracycline was monitored by the change in signal intensity. Under optimized conditions, the developed electrochemical strategy with MoS2-thionine composite and aptamer exhibited an excellent linear detection range of 1.0 nM-1.0 mu M and a low detection limit of 0.6 nM with suitable selectivity and stability. Therefore, the fabricated platform offers great potential for food safety, medical detection, and drug analysis.
期刊：
European Journal of Forest Research,2023年142(4):823-835 ISSN：1612-4669
通讯作者：
Yan, W.;Chen, X.
作者机构：
[Lei, Junjie; Li, Rujie; Yan, Wende] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Natl Engn Lab Appl Forest Ecol Technol Southern Ch, 498 South Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.;[Peng, Yuanying] Lewis Univ, Coll Arts & Sci, Romeoville, IL 60446 USA.;[Cao, Juan] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China.;[Zhou, Ting; Shi, Xiao; Jia, Qiang] China Int Engn Consulting Corp, Ecol Tech Res Inst, Beijing 100085, Peoples R China.;[Chen, Xiaoyong] Governors State Univ, Coll Arts & Sci, One Univ Pkwy, University Pk, IL 60484 USA.
通讯机构：
[Wende Yan] N;[Xiaoyong Chen] C;National Engineering Laboratory for Applied Forest Ecological Technology in Southern China, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China<&wdkj&>College of Arts and Sciences, Governors State University, University Park, USA
关键词：
Phosphorus fractions;Evergreen broad-leaved forest;Chinese fir plantation;Seasonal patterns
通讯机构：
[Zeng, CZ ] C;[Yan, ML ; Tong, JH ] H;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;Hunan Agr Univ, Coll Biosci & Biotechnol, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha 410128, Peoples R China.;Hunan Acad Agr Sci, Crop Res Inst, Changsha 410125, Peoples R China.
摘要：
Metabolic profiles in xylem sap are considered a fundamental mechanism for Cd detoxification in plants. However, the metabolic mechanism of Brassica juncea xylem sap in response to Cd is still unclear. Here, we investigated the effects on the metabolomics of B. juncea xylem sap treated with Cd at different times by utilizing a non-targeted LC-MS-based metabolomics method for further elucidating the response mechanism of Cd exposure. The findings indicated that 48 h and 7 d Cd exposure caused significant differences in metabolic profiles of the B. juncea xylem sap. Those differential metabolites are primarily involved in amino acids, organic acids, lipids, and carbohydrates, and most of them were downregulated, which played essential roles in response to Cd stress. Furthermore, B. juncea xylem sap resisted 48 h Cd exposure via regulation of glycerophospholipid metabolism, carbon metabolism, aminoacyl-tRNA biosynthesis, glyoxylate and dicarboxylate metabolism, linoleic acid metabolism, C5-branched dibasic acid metabolism, alpha-linolenic acid metabolism, cyanoamino acid metabolism, ABC transporters, biosynthesis of amino acids, and pyrimidine metabolism; whereas alpha-linolenic acid metabolism, glycerophospholipid metabolism, photosynthesis, and oxidative phosphorylation were regulated for resisting 7 d Cd exposure. This article is protected by copyright. All rights reserved.
摘要：
Diagnosing the spatial interaction between urbanization and ecosystem services (ES) is of guiding significance for regional sustainable development. The complex spatial interactions mechanism between different types of ur-banization and ES during historical and future periods were rarely explored at the whole watershed scale. To fill this gap, we systematically evaluated, predicted and compared the complex spatial interaction relationship between different types of urbanization and ES in the Yangtze River Basin (YRB) during 1990-2019 and in 2050 under different Shared Socioeconomic Pathways (SSPs) and Representative Concentration Pathways (RCPs) scenarios. Results showed that economic and land urbanization in the YRB will increase sharply during 1990-2050, while the population urbanization will drop slightly during 2019-2050. More than 59% of the YRB will experience an increase in total ecosystem services (TES) in SSP1-2.6 and SSP2-4.5 scenarios during 2019-2050. The average coupling coordination degree (CCD) between urbanization and TES in the YRB ranged 0.16-0.61 during 1990-2050, with a gradual increase from severe uncoordinated to basic coordination, and experienced a transition from lagging urbanization to lagging TES. More than 64% and 38% of the YRB will achieve strong decoupling between economic and population urbanization and TES during 1990-2050, respectively, which largely occur in the upper reaches, while over 34% of the YRB belong to weak decoupling between land urbanization and TES except for SSP1-2.6, which cluster in the middle and lower reaches. Moreover, we found that SSP1-2.6 will be the most promising scenario to achieve a win-win situation between different types of urbanization and TES, while prominent conflict between them will likely emerge in SSP5-8.5 scenario. The findings can contribute to further understanding the spatial interaction relationship between ur-banization and ES, and provide support for ecosystem protection and sustainable urban planning.
摘要：
Microplastic threats to biodiversity, health and ecological safety are adding to concern worldwide, but the real impacts on the functioning of organisms and ecosystems are obscure owing to their inert characteristics. Here we investigated the long-lasting ecological effects of six prevalent microplastic types: polyethylene (PE), polypropylene (PP), polyamide (PA), polystyrene (PS), polyethylene terephthalate (PET), and polyvinyl chloride (PVC) on soil bacteria at a 2 % (w/w) level. Due to the inertia and lack of available nitrogen of these microplastics, their effects on bacteriome tended to converge after one year and were strongly different from their short-term effects. The soil volumes around microplastics were very specific, in which the microplastic-adapted bacteria (e.g., some genera in Actinobacteria) were enriched but the phyla Bacteroidetes and Gemmatimonadetes declined, resulting in higher microbial nitrogen requirements and reduced organic carbon mineralization. The reshaped bacteriome was specialized in the genetic potential of xenobiotic and lipid metabolism as well as related oxidation, esterification, and hydrolysis processes, but excessive oxidative damage resulted in severe weakness in community genetic information processing. According to model predictions, microplastic effects are indirectly derived from nutrients and oxidative stress, and the effects on bacterial functions are stronger than on structure, posing a heavy risk to soil ecosystems.
摘要：
Floodplain wetlands are among the most dynamic ecosystems on Earth, featuring high biodiversity and productivity. They are also sensitive to anthropogenic disturbances and are globally threatened. Understanding how flow regime drives the spatiotemporal dynamics of wetland habitats is fundamental to effective conservation practices. In this study, using Landsat imagery and the random forest (RF) machine learning algorithm, we mapped the winter distribution of four wetland habitats (i.e., Carex meadow, reedbed, mudflat, and shallow water) in East Dongting Lake, a Ramsar wetland in the middle to lower Yangtze Basin of China, for 34 years (1988–2021). The dynamics of wetland habitats were explored through pixel-by-pixel comparisons. Further, the response of wetland habitats to flow regime variations was investigated using generalized additive mixed models (GAMM). Our results demonstrated the constant expansion of reedbeds and shrinkage of mudflats, and that there were three processes contributing to the reduction in mudflat: (1) permanent replacement by reedbed; (2) irreversible loss to water; and (3) transitional swapping with Carex meadow. These changes in the relative extent of wetland habitats may degrade the conservation function of the Ramsar wetland. Moreover, the duration of the dry season and the date of water level withdrawal were identified as the key flow regime parameters shaping the size of wetland habitats. However, different wetland vegetation showed distinct responses to variations in flow regime: while Carex meadow increased with earlier water withdrawal and a longer dry season, reedbed continuously expanded independent of the flow regime corresponding to the increase in winter rainfall. Our findings suggested that flow regime acts in concert with other factors, such as climate change and sand mining in river channels, driving wetland habitat transition in a floodplain landscape. Therefore, effective conservation can only be achieved through diverse restoration strategies addressing all drivers.
期刊：
Journal of Hazardous Materials,2023年441:129974 ISSN：0304-3894
通讯作者：
Haiyin Xu
作者机构：
[Xu, Haiyin; Luo, Yuanling] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;[Zhao, Xiaojing; Xu, Haiyin; Wang, Ping; Yang, Yi] Cent South Univ Forestry & Technol, Coll Environm Sci & Engn, Changsha 410004, Peoples R China.;[Luo, Yuanling] Changsha Environm Protect Coll, Changsha 410004, Peoples R China.;[Xie, Huaming; Zhou, Changsheng; Wang, Chuan] Pan China Construction Grp Co Ltd, Changsha 410004, Peoples R China.;[Liu, Zhiming] Eastern New Mex Univ, Dept Biol, Portales, NM 88130 USA.
通讯机构：
[Haiyin Xu] C;College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>College of Environmental Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
摘要：
Wastewater treatment plant (WWTP) has great potential to regulate microplastics (MPs) emissions, but few studies have focused on this subject. The present study performed a long-term investigation in a typically combined sewers WWTP. Compared to dry weather, rainfall contributed approximately 1.5 times more MPs to the inlet of the combined WWTP. The equalization tank served as a primary site for MPs' removal, with a 46.67-87.91 % removal rate. Decreasing surface overflow rate (SOR) was an effective measure for improving MPs removal, and a suitable SOR was recommended at similar to 11 m(3) (d.m(2))(-1)) in the equalization tank. However, MPs' existing settling/rising velocity models failed to obtain a theoretical SOR. In cyclic activated sludge systems, reducing sludge wastage was proposed to facilitate the removal of MPs. As a result, outlet MPs could be reduced by an order of magnitude by adjusting operational parameters on rainy days. Our study highlighted the potential for WWTP to operate as a controlled source of MPs by altering the operational parameters. We also advocated exploiting the mechanisms of MPs' removal by operational parameters and finding an equilibrium between MPs' removal and activated sludge performance.
作者：
Cao, Yini;Ma, Chuanxin;Yu, Hao;Tan, Qian;Dhankher, Om Parkash;...
期刊：
Journal of Hazardous Materials,2023年443(Pt B):130283 ISSN：0304-3894
通讯作者：
Qian Tan
作者机构：
[Cao, Yini; Ma, Chuanxin; Tan, Qian] Guangdong Univ Technol, Sch Ecol,Environm & Res, Key Lab City Cluster Environm Safety, Green Dev Minist Educ, Guangzhou 510006, Peoples R China.;[Cao, Yini] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yu, Hao] Univ Eastern Finland, Dept Environm & Biol Sci, POB 1672, Kuopio 70211, Finland.;[White, Jason C.] Connecticut Agr Expt Stn, New Haven, CT 06504 USA.;[Xing, Baoshan; Dhankher, Om Parkash] Univ Massachusetts, Stockbridge Sch Agr, Amherst, MA 01003 USA.
通讯机构：
[Qian Tan] K;Key Laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education, School of Ecology, Environment and Resources, Guangdong University of Technology, Guangzhou 510006, China
摘要：
Metal(loid)s contamination poses a serious threat to ecosystem biosafety and human health. Phytoremediation is a cost-effective and eco-friendly technology with good public acceptance, although the process does require a significant amount of time for success. To enhance the phytoremediation efficiency, numerous approaches have been explored, including soil amendments application with chelators to facilitate remediation. Sulfur (S), a macronutrient for plant growth, plays vital roles in several metabolic pathways that can actively affect metal(loid)s phytoextraction, as well as attenuate metal(loid) toxicity. In this review, different forms of S-amendments (fertilizers) on uptake and translocation in plants upon exposure to various metal(loid) are evaluated. Possible mechanisms for S application alleviating metal(loid) toxicity are documented at the physiological, biochemical and molecular levels. Furthermore, this review highlights the crosstalk between S-assimilation and other biomolecules, such as phytohormones, polyamines and nitric oxide, which are also important for metal(loid) stress tolerance. Given the effectiveness and potential of S amendments on phytoremediation, future studies should focus on optimizing phytoremediation efficiency in long-term field studies and on investigating the appropriate S dose to maximize the food safety and ecosystem health.
作者机构：
[Lei, Junjie; Yan, Wende; Zhang, Yi; He, Ping] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;[He, Ping] Forestry Bur Hunan Prov, Changsha 410004, Peoples R China.;[Yan, Wende] Natl Engn Lab Appl Forest Ecol Technol Southern Ch, Changsha 410004, Peoples R China.;[Peng, Yuanying] Lewis Univ, Coll Arts & Sci, Romeoville, IL 60446 USA.;[Zheng, Wei] Guangxi Forestry Res Inst, Nanning 530002, Peoples R China.
通讯机构：
[Chen, XY ] G;[Yan, WD ] C;Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;Natl Engn Lab Appl Forest Ecol Technol Southern Ch, Changsha 410004, Peoples R China.;Governors State Univ, Coll Arts & Sci, University Pk, IL 60484 USA.
关键词：
camphor forest;root respiration;microbial respiration;carbon cycling;soil CO2 efflux
摘要：
On a global scale, soil respiration (R-s), representing the CO2 flux between the soil surface and the atmosphere, ranks as the second-largest terrestrial carbon (C) flux. Understanding the dynamics between R-s and its autotrophic (R-a) and heterotrophic (R-h) components is necessary for accurately evaluating and predicting global C balance and net ecosystem production under environmental change. In this study, we conducted a two-year root exclusion experiment in subtropical China's Camphor (Cinnamomum camphora (L.) Presl.) forests to assess seasonal changes in R-a and R-h and their relative contributions to R-s. Additionally, we examined the influence of environmental factors on the dynamics of R-a, R-h, and R-s. Our results showed that seasonal mean R-s values were 2.88 mu mol m(-2) s(-1), with mean R-a and R-h of 1.21 and 1.67 mu mol m(-2) s(-1), respectively, in the studied forests. On an annual basis, the annual values of mean R-s in the studied forests were 405 +/- 219 g C m(-2) year(-1), with R-h and R-a accounting for 240 +/- 120 and 164 +/- 102 g C m(-2) year(-1), respectively. The seasonal mean ratio of R-h to R-s (R-h/R-s) was 58%, varying from 45 to 81%. Seasonal changes in R-s and R-h were strongly correlated with soil temperature but not soil water content. Both R-h and R-s increased exponentially with the average soil temperature measured in the topsoil layer (about 5 cm), with Q(10) values of 2.02 and 1.73 for R-h and R-s, respectively. Our results suggest that the composition and activity of soil microbes and fauna play a primary role in releasing carbon flux from soil to the atmosphere in the studied forest ecosystems.
通讯机构：
[Liu, Q ] C;[Yang, Y ] H;Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha 410004, Peoples R China.;Hunan Acad Forestry, State Key Lab Utilizat Woody Oil Resource, Changsha 410004, Peoples R China.
关键词：
Gardenia jasminoides;geniposide;crocin;medicinal ingredients;synthesis pathway;fruit development
摘要：
Gardenia jasminoides fruits are extensively grown worldwide, with a large harvest, and its major medicinal ingredients are geniposide and crocins. Research on their accumulation and biosynthsis-related enzymes is rare. In this study, the accumulation of geniposide and crocin of G. jasminoides fruits at different developmental stages were clarified by HPLC. The highest cumulative amount of geniposide was 2.035% during the unripe-fruit period, and the highest content of crocin was 1.098% during the mature-fruit period. Furthermore, transcriptome sequencing was performed. A total of 50 unigenes encoding 4 key enzymes related in geniposide biosynthsis pathways were screened, and 41 unigenes encoding 7 key enzymes in the pathways of crocin were elucidated. It was found that the expression levels of differentially expressed genes of DN67890_c0_g1_i2-encoding GGPS, which is highly related to geniposide biosynthesis, and DN81253_c0_g1_i1-encoding lcyB, DN79477_c0_g1_i2-encoding lcyE, and DN84975_c1_g7_i11-encoding CCD, which are highly related to crocin biosynthesis, were consistent with the accumulation of geniposide and crocin content, respectively. The qRT-PCR results showed that the trends of relative expression were consistent with transcribed genes. This study provides insights for understanding the geniposide and crocin accumulation and biosynthsis during fruit development in G. jasminoides.
作者机构：
[Zhang, Youming; Li, Ruijuan; Zheng, Wentao; Wang, Xue; Jiang, Chanjuan; Li, RJ; Fu, Jun; Fu, J; Gao, Shiqing; Tu, Qiang; Ravichandran, Vinothkannan] Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China.;[Zhou, Diao; Yin, Yulong; Xia, Yandong; Yin, Jia] Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.;[Xia, Yandong] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Key Lab Natl Forestry Grassland & Adm Control Arti, Hunan Prov Key Lab Control Forest Dis & Pests,Key, Changsha, Peoples R China.;[Zhang, Youming; Tu, Qiang] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen, Peoples R China.
通讯机构：
[Yin, J ] H;[Li, RJ ; Fu, J] S;Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China.;Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.
摘要：
A lack of generic and effective genetic manipulation methods for Pseudomonas has restricted fundamental research and utilization of this genus for biotechnology applications. Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome engineering. This PEHR system is based on a lambda Red-like operon (BAS) from Pseudomonas aeruginosa phage Ab31 and a Rac bacteriophage RecET-like operon (Rec-TEPsy) from P. syringae pv. syringae B728a and also contains exogenous elements, including the RecBCD inhibitor (Redγ or Pluγ) or single-stranded DNA-binding protein (SSB), that were added to enhance the PEHR recombineering efficiency. To solve the problem of false positives in Pseudomonas editing with the PEHR system, the processive enzyme Cas3 with a minimal Type I-C Cascade-based system was combined with PEHR. This protocol describes the utilization of a Pseudomonas-specific PEHR–Cas3 system that was designed to universally and proficiently modify the genomes of Pseudomonas species. The pipeline uses standardized cassettes combined with the concerted use of SacB counterselection and Cre site-specific recombinase for markerless or seamless genome modification, in association with vectors that possess the selectively replicating template R6K to minimize recombineering background. Compared with the traditional allelic exchange editing method, the PEHR–Cas3 system does not need to construct suicide plasmids carrying long homologous arms, thus simplifying the experimental procedure and shortening the traceless editing period. Compared with general editing systems based on phage recombinases, the PEHR–Cas3 system can effectively improve the screening efficiency of mutants using the cutting ability of Cas3 protein. The entire procedure requires ~12 days. This protocol for universal and proficient Pseudomonas recombineering uses phage-encoded homologous recombination–Cas3 systems, including SacB counterselection and Cre site-specific recombinase for two- or three-step seamless genome modification.
摘要：
Patulin (PAT), a fungal metabolite, which mainly exists in various moldy fruits, is greatly harmful to human. However, there are still many unsolved problems in the current detection of PAT. In the work, an ultra-highly sensitive and effective monitoring strategy for PAT was explored based on a novel electrochemiluminescence (ECL) aptasensor. Here, 3, 4, 9, 10-perylenetetracarboxylic acid (PTCA) was chosen as luminophore and terbium (Tb) metal organic gel (MOG) was prepared as a co-reaction accelerator. The MOG displayed a large surface area for immobilization of sufficient PTCA to catalyze co-reactant K2S2O8 to generate more sulfate radical anions (SO4 & BULL; -), thus accelerating the electron-transfer (ET) rate to facilitate ECL efficiency. In the presence of PAT, the ECL signal was greatly quenched, which could be attributed to the specific binding effect between PAT aptamer and PAT. Based on this process, a wide range of PAT concentrations from 0.1 fg & BULL;mL-1 to 0.1 & mu;g & BULL;mL-1 and a low detection limit of 0.02 fg & BULL;mL-1 (S/N = 3) were analyzed. The aptasensor showed excellent detection properties for trace patulin in terms of selectivity, sensitivity, stability, reproducibility and practicability. The designing ideas could serve as a model for the application of metal organic gels to design ultra-highly sensitive ECL platforms for food safety.
作者：
Ning, Ying;Liu, Shuguang*;Smith, Andrew R.;Qiu, Yi;Gao, Haiqiang;...
期刊：
Science of The Total Environment,2023年863:160705 ISSN：0048-9697
通讯作者：
Liu, Shuguang
作者机构：
[Liu, Shuguang; Ning, Ying; Feng, Shuailong; Gao, Haiqiang] Cent South Univ Forestry & Technol CSUFT, Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;[Liu, Shuguang; Ning, Ying; Feng, Shuailong; Gao, Haiqiang] Coll Life Sci & Technol, CSUFT, Changsha 410004, Peoples R China.;[Smith, Andrew R.] Bangor Univ, Environm Ctr Wales, Sch Nat Sci, Bangor LL57 2UW, Wales.;[Qiu, Yi] Coll Business, CSUFT, Changsha 410004, Peoples R China.;[Lu, Yonglong] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Urban & Reg Ecol, Beijing 100085, Peoples R China.
通讯机构：
[Shuguang Liu] N;National Engineering Laboratory for Applied Technology of Forestry & Ecology in South China, Central South University of Forestry and Technology (CSUFT), Changsha 410004, China<&wdkj&>College of Life Science and Technology, CSUFT, Changsha 410004, China
关键词：
Cross-sectional scaling;Power law;Temporal scaling;The evolution of cities;Urban growth;Urban sustainability
摘要：
Understanding the co-evolution and organizational dynamics of urban properties (i.e., urban scaling) is the science base for pursuing synergies toward sustainable cities and society. The generalization of urban scaling theory yet requires more studies from various developmental regimes and across time. Here, we extend the universality proposition by exploring the evolution of longitudinal and transversal scaling of Chinese urban attributes between 1987 and 2018 using a global artificial impervious area (GAIA) remotely sensed dataset, harmonized night light data (NTL), and socioeconomic data, and revealed agreements and disagreements with theories. The superlinear relationship of urban area and population often considered as an indicator of wasting land resources (challenging the universality theory β(c) = 2/3), is in fact the powerful impetus (capital raising) behind the concurrent superlinear expansion of socio-economic metabolisms (e.g., GDP, total wage) in a rapidly urbanizing country that has not yet reached equilibrium. Similarly, infrastructural variables associated with public services, such as hospitals and educational institutions, exhibited some deviations as well and were scaled linearly. However, the temporal narrowing of spatial deviations, such as the decline in urban land diseconomies of scale and the stabilization of economic output, clearly indicates the Chinese government's effort in charting urban systems toward balanced and sustainable development across the country. More importantly, the transversal sublinear scaling of areal-based socio-economic variables was inconsistent with the theoretical concept of increasing returns to scale, thus validating the view that a single measurement cannot unravel the intricate web of diverse urban attributes and urbanization. Our dynamic urban scaling analysis across space and through time in China provides new insights into the evolving nexus of urbanization, socioeconomic development, and national policies.
