作者机构：
[Sheng, Yuxiang; Feng, Jie; Wang, Ting; Chen, Liang; Wang, Guangjun; Lv, Hao; Ouyang, Shuai; Pan, Jinwen; Liu, Yue; Yang, Jie; Su, Siwen] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, 498 Southern Shaoshan Rd, Changsha 410004, Peoples R China.;[Lv, Hao] Hunan Bot Garden, Changsha 410116, Peoples R China.;[Sheng, Yuxiang; Feng, Jie; Wang, Ting; Chen, Liang; Wang, Guangjun; Ouyang, Shuai; Pan, Jinwen; Liu, Yue; Yang, Jie; Su, Siwen] Huitong Natl Stn Sci Observat & Res Chinese Fir Pl, Huaihua 438107, Huitong, Peoples R China.;[Tang, Li] Hunan Appl Technol Univ, Fac Agr & Forestry Technol, Changde 415000, Peoples R China.
通讯机构：
[Wang, GJ ] C;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, 498 Southern Shaoshan Rd, Changsha 410004, Peoples R China.;Huitong Natl Stn Sci Observat & Res Chinese Fir Pl, Huaihua 438107, Huitong, Peoples R China.
摘要：
Although many studies have focused on the roles of soil microbes in phosphorus (P) cycling, little is known about the distribution of microbial P cycling genes across soil depths. In this study, metagenomic sequencing was adopted to examine the differences in the abundance of genes and microbial taxa associated with soil P cycling between organic and mineral soil in subtropical forests. The total relative abundance of inorganic P solubilizing genes was the highest, that of P starvation response regulating genes was second, and organic P mineralizing genes was the lowest. The soil organic carbon concentration, N:P ratio, and available P concentration were higher in the organic soil than the mineral soil, resulting in abundances of organic P mineralizing genes (appA and 3-phytase), and inorganic P cycling genes (ppa), whereas those of the inorganic P cycling genes (gcd and pqqC) and the P starvation response regulating gene (phoR) were higher in mineral soil. The four bacteria phyla that related to P cycling, Proteobacteria, Actinobacteria, Bacteroidetes, and Candidatus_Eremiobacteraeota were higher in organic soil; conversely, the three bacteria phyla (Acidobacteria, Verrucomicrobia, and Chloroflexi) and archaea taxa were more abundant in mineral soil. Therefore, we concluded that the distribution of genes and microbial taxa involved in soil P cycling differed among soil depths, providing a depth-resolved scale insight into the underlying mechanisms of P cycling by soil microorganisms in subtropical forests.
期刊：
Agricultural and Forest Meteorology,2023年329:109291 ISSN：0168-1923
通讯作者：
Wenhua Xiang
作者机构：
[Ouyang, Shuai; Hu, Yanting; Lei, Pifeng; Chen, Liang; Zhao, Zhonghui; Xiang, Wenhua; Zeng, Yelin; Fang, Xi; Deng, Xiangwen] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Ouyang, Shuai; Hu, Yanting; Lei, Pifeng; Chen, Liang; Zhao, Zhonghui; Xiang, Wenhua; Zeng, Yelin; Fang, Xi; Deng, Xiangwen] Huitong Natl Stn Sci Observat & Res Chinese Fir Pl, Huitong 438107, Hunan, Peoples R China.;[Sun, Zhihui] China Mobile Hangzhou Informat Technol Co Ltd, Hangzhou 311100, Zhejiang, Peoples R China.;[Xiang, Wenhua] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, 498 Southern Shaoshan Rd, Changsha 410004, Peoples R China.
通讯机构：
[Wenhua Xiang] F;Faculty of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China<&wdkj&>Huitong National Station for Scientific Observation and Research of Chinese Fir Plantation Ecosystems in Hunan Province, Huitong, Hunan 438107, China
摘要：
Lactarius hatsudake Tanaka is a mycorrhizal edible mushroom with rich economic and nutritional value. Although it is artificially planted, its yield is unstable. Soil fungi, including L. hatsudake, coexist with many other microorganisms and plants. Therefore, complex microbial communities have an influence on the fruiting body formation of L. hatsudake. L. hatsudake and its interactions with the rest of the fungal community over time are not completely understood. In this study, we performed high-throughput sequencing of microorganisms in the basal soil of the fruiting body (JT), mycorrhizosphere soil (JG), and non-mushroom-producing soil (CK) in a 6-year-old L. hatsudake plantation at harvest. The results showed that the soil of the L. hatsudake plantation was rich in fungal communities and a total of 10 phyla, 19 classes, 53 orders, 90 families, 139 genera, and 149 species of fungi were detected. At the phylum level, the major groups were Basidiomycota and Ascomycota. At the genus level, the dominant groups were Lactarius, Trichoderma, Suillus, and Penicillium. Among them, L. hatsudake had an absolute dominant position in the soil fungal community of the plantation, and was the only group of Lactarius in the plantation soil. Penicillium cryptum and Penicillium adametzii were unique to the JT soil sample. Chaetopsphaeria, Myxocephala, Devriesia, and Psathyrella were positively correlated with L. hatsudake. In the constructed fungal network, the total number of nodes were ranked in descending order as JG (441) > CK (405) > JT (399), while the total number of edges were ranked in descending order as CK (1360) > JG (647) > JT (586). Analysis of the fungal assembly process revealed that groups CK and JG have determinative processes that dominated community building, while the JT group exhibited a dominant random process with a 0.60 probability. The results indicated that L. hatsudake was successfully colonized in the plantation soil. During harvest, the CK group exhibited the largest network size and the most complex fungal interactions, while the fungal community structure in the mushroom cultivation zone (JT and JG) was stable and less susceptible to external environmental interference. L. hatsudake affects the fungal community in the soil surrounding its fruiting body.
作者机构：
[Hassan Farooq T.] Bangor College China, a Joint Unit of Bangor University and Central South University of Forestry and Technology, Hunan, Changsha, 410004, China;[Jabeen S.] Department of Botany, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan;[Shakoor A.] Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW 2751, Australia;[Saleem Arif M.] Department of Environmental Sciences, Government College University Faisalabad, Faisalabad, 38000, Pakistan;[Umair Riaz M.; Siddique N.] Department of Forestry, Range, and Wildlife Management, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan
通讯机构：
[Taimoor Hassan Farooq] B;Bangor College China, a Joint Unit of Bangor University and Central South University of Forestry and Technology, Changsha 410004, Hunan, China
作者：
Tang, Wenxi;Liu, Shuguang;Feng, Shuailong;Xiao, Fangmeng;Ogbodo, U. S.
期刊：
Science of The Total Environment,2023年858(Pt 1):159715 ISSN：0048-9697
作者机构：
[Liu, Shuguang; Tang, Wenxi; Ogbodo, U. S.; Xiao, Fangmeng; Feng, Shuailong] Cent South Univ Forestry & Technol CSUFT, Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;[Liu, Shuguang; Tang, Wenxi; Ogbodo, U. S.; Xiao, Fangmeng; Feng, Shuailong] CSUFT, Coll Life Sci & Technol, Changsha 410004, Peoples R China.
关键词：
Ecological environment quality;Urban Green Heart;Urban agglomeration;Urbanization;Ecological environment assessment
摘要：
Large ecological green spaces in cities are often designated as Urban Green Hearts (GHs) to support the ecological and recreational needs of urbanites. While GHs protection and sustainable development have been a high priority for urban planning and management, ecological environment quality (EEQ) of GHs has rarely been monitored and assessed. Here, we proposed a comprehensive assessment framework for EEQ based on entropy weights and rank-sum ratios methods, and applied the framework to the world's largest GH, Changsha-Zhuzhou-Xiangtan urban agglomeration Green Heart (CZT-GH), and its 5 km and 10 km buffer zones to examine the spatial-temporal dynamics of its EEQ from 2000 to 2019. Compared with the buffer zones, the EEQ in the CZT-GH was the best, with an annual average of 44.92 % of the area being High-grades EEQ. The restoration trend of EEQ was most conspicuous in only 8.4 % of CZT-GH, a small fraction compared with 25.1 % and 66.5 % of the CZT-GH showing deterioration trend and no change, respectively. Five factors were identified that calls for management attention: land use and cover change, spatial heterogeneity in vegetation restoration, temporal fluctuation in air quality improvement, comprehensive EEQ assessment and restoration, and capacity to cope with ecological risks. The approach, issues identified, and management measures proposed in this study should be applicable to GHs in general. The generic EEQ assessment framework and approaches developed in this study are generic and objective and therefore can be easily adapted to other regions; the procedures used to quantify the spatial and temporal changes of EEQ and identify underlying management issues provide essential information for formulating adaptive management measures of EEQ in general. SYNOPSIS: Taking the largest urban Green Heart as a case study, we established and applied a new general ecological environment quality (EEQ) evaluation system to monitor EEQ changes, identify issues, and propose management options.
作者机构：
[Ouyang, Shuai; Hu, Yanting; Chen, Liang; Sun, Shulan; Zhao, Zhonghui; Xiang, Wenhua; Zeng, Yelin] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Ouyang, Shuai; Hu, Yanting; Chen, Liang; Sun, Shulan; Zhao, Zhonghui; Xiang, Wenhua] Huitong Natl Stn Sci Observat & Res Chinese Fir Pl, Huitong 438107, Hunan, Peoples R China.;[Liu, Meixian] Sun Yat sen Univ, Sch Civil Engn, Zhuhai 519082, Peoples R China.;[Zhou, Xiaolu; Peng, Changhui] Hunan Normal Univ, Sch Geog Sci, Changsha 410081, Peoples R China.;[Peng, Changhui] Univ Quebec Montreal, Inst Environm Sci, Dept Biol Sci, Case Postale 8888,Succ Ctr Ville, Montreal, PQ 338, Canada.
通讯机构：
[Wenhua Xiang] F;Faculty of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan, 410004, China<&wdkj&>Huitong National Station for Scientific Observation and Research of Chinese Fir Plantation Ecosystems in Hunan Province, Huitong, Hunan, 438107, China
期刊：
Methods in Molecular Biology,2023年2594:13-28 ISSN：1064-3745
作者机构：
[Wu, Qiang] College of Food and Chemical Engineering, Shaoyang University, Shaoyang, China. qiangwusmile@163.com;[Li, Qiang; Ma, Jiang-Shan; Liu, Gao-Qiang; Wu, Qiang] Hunan Provincial Key Laboratory of Forestry Biotechnology &[Wu, Qiang] International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, China. qiangwusmile@163.com;[Wu, Qiang] Microbial Variety Creation Center, National Laboratory of Yuelushan Seed Industry, Changsha, China. qiangwusmile@163.com;[Li, Qiang; Ma, Jiang-Shan; Liu, Gao-Qiang] International Cooperation Base of Science and Technology Innovation on Forest Resource Biotechnology, Central South University of Forestry and Technology, Changsha, China
关键词：
DNA pull-down;EMSA;Gene knockout;Transcription factor
摘要：
This protocol describes a method for verifying the specific transcription factor regulating glycerol dehydratase (GDH) expression in Klebsiella. DNA pull-down accompanied with mass spectrometry is used to screen and identify the transcription factor interacting with the promoter region of the key gene in Klebsiella. EMSA method is used to validate the specific binding of the transcription factor to the promoter region in vitro. In addition, the target DNA fragments are constructed by fusion PCR to prepare competent cells from Klebsiella for electrical transformation and further transformed to obtain key gene deletion strains to verify the transcription factor responsible for the target gene expression in Klebsiella.
通讯机构：
[Yong Li] N;National Engineering Laboratory for Applied Forest Ecological Technology in Southern China, Laboratory of Urban Forest Ecology of Hunan Province, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
摘要：
The increased emission and accumulation of micro- or nanoplastics (M-NPs) have posed a severely threaten to organisms in the environment. Though the toxicity of M-NPs has been observed in many species, the fundamental factors determining the biotoxicity are rarely expounded on. In this work, typical polystyrene (PS) M-NPs were set up with a multiparameter variation in size gradient, surface charge contrast and concentration variant, and evaluated by the Caenorhabditis elegans (C. elegans) model. From the endpoints of body length, brood size, survival rate and lifespan, an adverse effect was found on the growth and development of C. elegans caused by PSs. In general, the toxicity of PS was found to be concentrated- and size-dependent, with 100 nm positively charged nano-PS having the highest physio-toxicity. Monitoring by fluorescent imaging, it showed that positively charged nano-PS was mainly ingested and accumulated in the intestinal tract of C. elegans. In addition, the penetrated PS induced severe biological stress reactions with the increase of reactive oxygen species (ROS) and lipofuscin. Furthermore, the following expression of antioxidation-related enzymes was activated in vivo as indicated by the GFP-labelled C. elegans. All the results supplied visually toxic parameters of M-NPs to organisms, which sheds light on the biosecurity and ecological risks of M-NPs in the future.
作者机构：
[Lei, Junjie; Chen, Yazhen; Zheng, Wei; Xu, Yichen; Yan, Wende; Jiang, Xingjian] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Peoples R China.;[Lei, Junjie; Chen, Yazhen; Zheng, Wei; Xu, Yichen; Yan, Wende; Jiang, Xingjian] Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;[Lei, Junjie; Chen, Yazhen; Zheng, Wei; Xu, Yichen; Yan, Wende; Jiang, Xingjian] Natl Observat Res Stn Forest Ecosyst Lutou Forest, Yueyang 414000, Hunan, Peoples R China.;[Dang, Peng] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Peoples R China.;[Yan, Wende] Key Lab Urban Forest Ecol Hunan Prov, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Wende Yan] F;Faculty of Life Science and Technology, Central South University of Forestry & Technology, Changsha 410004, China<&wdkj&>National Engineering Laboratory for Applied Technology of Forestry & Ecology in South China, Changsha 410004, China<&wdkj&>National Observation research Station of Forest Ecosystem of Lutou Forest Farm in Hunan Province, Yueyang 414000, Hunan, China<&wdkj&>Key Laboratory of Urban Forest Ecology of Hunan Province, Changsha 410004, Hunan, China<&wdkj&>Key Laboratory of Subtropical Forest Ecology of Hunan Province, Changsha 410004, Hunan, China
关键词：
Community assembly;Elevation;Fallen wood;Partial least squares path model;Soil fungal diversity
通讯机构：
[Chen, L; Xiang, WH ] C;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha, Hunan, Peoples R China.;Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha, Peoples R China.
关键词：
Microbial communities and functions;Nutrient cycling;Plantation management;Soil functions;Thinning intensity
摘要：
Thinning is a widely-used management practice to reduce tree competition and improve wood production and quality in forest plantations. Thinning affects the soil ecosystem by changing the microclimate and plant growth, as well as litter inputs above and belowground, with all the resulting consequences for microbial communities and functions. Although many case studies have been carried out, a comprehensive understanding of the thinning effects on soil properties and microbial communities and functions in plantations remains to be explored. In this study, a meta-analysis was performed on 533 paired observations based on 90 peer-reviewed articles to evaluate the general responses of soil (mainly 0-20cm depth) physicochemical properties, microbial biomass and community structure, and enzyme activities to thinning. Results showed that thinning increased soil temperature (13%), moisture (8.0%), electric conductivity (13%), and the contents of total nitrogen (TN, 4.1%), dissolved organic carbon (DOC, 9.7%), nitrate N (NO(3)(-)-N, 27%) and available phosphorous (22%). For microbial properties, thinning decreased the fungi to bacteria ratio (F:B, -28%) and the gram-positive bacteria to gram-negative bacteria ratio (G+:G-, -12%), while increased microbial biomass C (7.1%), microbial respiration (13%), and nutrient-cycle related enzyme activities, including phenol oxidase (14%), cellobiohydrolase (21%), urease (10%), and acid phosphatase (9%). In particular, moderate thinning (30-60% intensity) has higher conservation benefits for soil C and nutrients than light and heavy intensity, thus being recommended as the optimal thinning activity. This meta-analysis suggests that thinning consistently altered soil properties, shifted microbial community compositions from K- to-r strategist dominance, and stimulated microbial activities. These results are essential for optimizing plantation thinning management and provide evidence for applying the macro-ecology theory to ecosystem disturbance in soil microbial ecology.
通讯机构：
[Xiang, W.] F;Faculty of Life Science and Technology, China
关键词：
Abiotic and biotic factors;Biodiversity;Functional composition;Functional traits;Soil microbial diversity;Stand structure
摘要：
Tree species diversity is assumed to be an important component in managing forest ecosystems because of effects on multiple functions or ecosystem multifunctionality. However, the importance of tree diversity in determining multifunctionality in structurally complex subtropical forests relative to other regulators (e.g., soil microbial diversity, stand structure, and environmental conditions) remains uncertain. In this study, effects of aboveground (species richness and functional and structural diversity) and belowground (bacterial and fungal diversity) biodiversity, functional composition (community-weighted means of species traits), stand structure (diameter at breast height and stand density), and soil factors (pH and bulk density) on multifunctionality (including biomass production, carbon stock, and nutrient cycling) were examined along a tree diversity gradient in subtropical forests. The community-weighted mean of tree maximum height was the best predictor of ecosystem multifunctionality. Functional diversity explained a higher proportion of the variation in multifunctionality than that of species richness and fungal diversity. Stand structure -played an important role in modulating the effects of tree diversity on multifunctionality. The work highlights that species composition and maximizing forest structural complexity are effective strategies to increase forest multifunctionality while also conserving biodiversity in the management of multifunctional forests under global environmental changes.
期刊：
Ecotoxicology and Environmental Safety,2023年249:114458 ISSN：0147-6513
通讯作者：
Chen, L.;Pan, Y.
作者机构：
[Chen, Zekai; Zheng, Wei; Pan, Yuliang; Yan, Wende; Tang, Jun; Hu, Lei] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Peoples R China.;[Chen, Zekai; Zheng, Wei; Pan, Yuliang; Yan, Wende; Tang, Jun; Hu, Lei] Natl Engn Lab Appl Technol Forestry & Ecol South C, Changsha 410004, Peoples R China.;[Chen, Zekai; Zheng, Wei; Pan, Yuliang; Yan, Wende; Tang, Jun; Hu, Lei] Lab Urban Forest Ecol Hunan Prov, Changsha 410004, Hunan, Peoples R China.;[Chen, Lijun] Cent South Univ Forestry & Technol, Fac Forestry, Changsha 410004, Peoples R China.;[Chen, Xiaoyong] Governors State Univ, Coll Arts & Sci, University Pk, IL 60484 USA.
通讯机构：
[Pan, Y.; Chen, L.] F;Faculty of Life Science and Technology, Central South University of Forestry & Technology, Changsha 410004, China<&wdkj&>National Engineering Laboratory for Applied Technology of Forestry & Ecology in South China, Changsha 410004, China<&wdkj&>Laboratory of Urban Forest Ecology of Hunan Province, Changsha, Hunan 410004, China
关键词：
Perfluorinated compounds;Bacterial and fungal communities;PFOA;PFOS
