Nondestructive and Quantitative Analysis of Cell Wall Regeneration in the Medicinal Macrofungus Ganoderma lingzhi by a Membrane-Fusing Fluorescent Probe

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期刊论文

作者：

Shi, Muling;Mi, Xujun;Huang, Ling;Qiu, Liping;Yang, Liu;...

通讯作者：

Liu, GQ

作者机构：

[Liu, GQ; Yang, Yingzhuo; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.

[Liu, GQ; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang; Yang, Liu] Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.

[Sun, Xing; Shi, Muling; Qiu, Liping; Yang, Liu] Hunan Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Chem & Mol Med, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.

[Shi, Muling; Hong, Chao] Hainan Univ, Coll Mat Sci & Engn, Haikou 570228, Hainan, Peoples R China.

通讯机构：

[Liu, GQ ] C

Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.

Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.

语种：

英文

期刊：

Analytical Chemistry

ISSN：

0003-2700

年：

2023

卷：

期：

页码：

8357-8366

DOI：

10.1021/acs.analchem.3c01016

基金类别：

The authors acknowledge the financial support from the Natural Science Foundation of Hunan Province, grant numbers 2020JJ5976 and 2021JJ31151; the China Postdoctoral Science Foundation, grant number 2020M682536; the National Natural Science Foundation of China, grant number 32071673, the Program of Hunan Science and Technology Innovation Team, grant number 2021RC4063, and the Scientific Research Project of Education Department of Hunan Province for Distinguished Young Scholars, grant number 18B179. The authors appreciated Xueqiang Wang, Yuting Zhuo, and Yongnan Liu for their kind suggestions about this work.

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本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

Ganoderma is a prize medicinal macrofungus with a broad range of pharmaceutical values. To date, various attempts have been made to cultivate Ganoderma to improve the production of secondary metabolites with pharmacological activity. Among the adopted techniques, protoplast preparation and regeneration are indispensable. However, the evaluation of protoplasts and regenerated cell walls usually relies on electron microscopy assays, which require time-consuming and destructive sample preparation and merely provide localized information in the sel...

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Nondestructive and Quantitative Analysis of Cell Wall Regeneration in the Medicinal Macrofungus Ganoderma lingzhi by a Membrane-Fusing Fluorescent Probe

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