Nondestructive and Quantitative Analysis of Cell Wall Regeneration in the Medicinal Macrofungus Ganoderma lingzhi by a Membrane-Fusing Fluorescent Probe

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期刊论文

作者：

Shi, Muling;Mi, Xujun;Huang, Ling;Qiu, Liping;Yang, Liu;...

通讯作者：

Liu, GQ

作者机构：

[Liu, GQ; Yang, Yingzhuo; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.

[Liu, GQ; Shi, Muling; Huang, Ling; Wang, Xiaoling; Mi, Xujun; Liu, Gao-Qiang; Yang, Liu] Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.

[Sun, Xing; Shi, Muling; Qiu, Liping; Yang, Liu] Hunan Univ, Coll Chem & Chem Engn, Collaborat Innovat Ctr Chem & Mol Med, Coll Biol,State Key Lab Chemo Biosensing & Chemome, Changsha 410082, Peoples R China.

[Shi, Muling; Hong, Chao] Hainan Univ, Coll Mat Sci & Engn, Haikou 570228, Hainan, Peoples R China.

通讯机构：

[Liu, GQ ] C

Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.

Cent South Univ Forestry & Technol, Yuelu Mt Hunan Prov Lab, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha 410004, Hunan, Peoples R China.

语种：

英文

期刊：

Analytical Chemistry

ISSN：

0003-2700

年：

2023

卷：

期：

页码：

8357-8366

DOI：

10.1021/acs.analchem.3c01016

基金类别：

Natural Science Foundation of Hunan Province [2020JJ5976, 2021JJ31151]; China Postdoctoral Science Foundation [2020M682536]; National Natural Science Foundation of China [32071673]; Program of Hunan Science and Technology Innovation Team [2021RC4063]; Scientific Research Project of Education Department of Hunan Province for Distinguished Young Scholars [18B179]

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本校为第一且通讯机构

院系归属：

生命科学与技术学院

摘要：

Ganoderma is a prize medicinal macrofunguswitha broad range of pharmaceutical values. To date, various attemptshave been made to cultivate Ganoderma to improvethe production of secondary metabolites with pharmacological activity.Among the adopted techniques, protoplast preparation and regenerationare indispensable. However, the evaluation of protoplasts and regeneratedcell walls usually relies on electron microscopy assays, which requiretime-consuming and destructive sample preparation and merely providelocalized information in the selected area. In contrast, fluorescenceassays enable sensitiv...

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Nondestructive and Quantitative Analysis of Cell Wall Regeneration in the Medicinal Macrofungus Ganoderma lingzhi by a Membrane-Fusing Fluorescent Probe

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