期刊论文

中文

中草药

0253-2670

2023

54

16

5350-5357

10.7501/j.issn.0253-2670.2023.16.024

2022JJ30326:湖南省自然科学基金面上项目 XLKY202208:湖南省林业科技创新资金项目 2020JJ4939:湖南省自然科学基金项目

本校为第一机构

目的克隆获得灰毡毛忍冬Lonicera macranthoides MADS-box家族基因SHORT VEGETATIVE PHASE(SVP),并对其进行生物信息学、时空表达特异性和蛋白原核表达等特性分析。方法根据灰...展开更多 目的克隆获得灰毡毛忍冬Lonicera macranthoides MADS-box家族基因SHORT VEGETATIVE PHASE(SVP),并对其进行生物信息学、时空表达特异性和蛋白原核表达等特性分析。方法根据灰毡毛忍冬转录组数据设计特异性引物,通过PCR技术克隆Lm SVP基因全长;运用生物信息学方法分析其编码蛋白的序列特征;利用实时荧光定量技术(reverse-transcription polymerase chain reaction,q RT-PCR)分别检测该基因在灰毡毛忍冬2个品种...

ObjectiveTo clone the MADS-boxfamily gene SHORT VEGETATIVE PHASE(SVP)of Lonicera macranthoides,analyze the characteristics of bioinformatics,spatiotemporal expression and protein prokaryotic expression.MethodsBased on the transcriptome sequencing data of L.macranthoides...MORE ObjectiveTo clone the MADS-boxfamily gene SHORT VEGETATIVE PHASE(SVP)of Lonicera macranthoides,analyze the characteristics of bioinformatics,spatiotemporal expression and protein prokaryotic expression.MethodsBased on the transcriptome sequencing data of L.macranthoidesin...