摘要：
Abstract Background Biochar is widely recognized for its capacity to capture and store carbon in soil attributed to its stable structure. However, in most field studies examining the effects of biochar application on soil respiration, the impact of rainfall events on the experimental outcomes has not been taken into account. To address the existing gap in this research field, we conducted a one-year study on soil respiration in an urban camphor forest and collected the data of soil respiration, soil temperature, soil moisture, and the rainfall events closest to the soil respiration monitoring time. We specifically examined how different stages of rainfall events influenced soil respiration in relation to biochar application. Results This study found that the annual average soil respiration rate increased with the doses of biochar application, and the soil respiration rate under the biochar application at the dose of 45 t/ha showed a significant rise. The stages of rainfall events, rainfall amount, and the interaction effect of the two, and biochar doses significantly affected soil respiration. The parameters in the regression model for soil respiration, soil temperature and moisture varied with the different stages of rainfall events and the doses of biochar application. The biochar application eliminated the significant effect of soil moisture on soil respiration during one day after rainfall events. The significant correlation between soil moisture and the temperature sensitivity of soil respiration (Q10) was eliminated by biochar application, both during one day after rainfall events and more than eight days after rainfall events. Conclusions Our findings indicated that the rice straw biochar application has a short-term positive effect on soil respiration in urban camphor forests. The rainfall events affect the field soil respiration monitored in the biochar applications, possibly by affecting the soil respiration response to soil temperature and moisture under different doses of biochar application. The impact of rainfall events on soil respiration in biochar application experiments should be considered in future forest monitoring management and practice.
摘要：
Ganoderma lingzhi is a new species of the prize medicinal mushroom Ganoderma (Agaricomycetes). Using angiotensin I-converting enzyme (ACE) as a target, a tripeptide Ser-Tyr-Pro (SYP) was discovered with preponderant ACE inhibitory activity with an 50% inhibiting concentration (IC50) value of 62.50 mu g/mL attribute to the formed salt bridge and hydrogen bonds between SYP and ACE. SYP even maintained superior bioactivity after intestinal digestion, and exerted no cytotoxicity, but presented incomplete bioavailability in blood of spontaneous hypertensive rats (SHRs). Furthermore, it performed antihypertensive effect in vivo by inhibiting the influx of Ca2+ through activating endothelial NO synthase (eNOS)/NO/guanosine 3',5'-cyclic monophosphate (cGMP) pathway, accompanied by attenuating angiotensin II (Ang II)/NADPH oxidase (NOX)/ reactive oxygen species (ROS) pathway. This work not only discoverers a novel pharmacological ingredient from medicinal mushroom G. lingzhi for hypertension therapy, but also provides an insight into molecular mechanism of the ACE inhibitory peptide (ACEIP) on lowering blood pressure. (c) 2025 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
通讯机构：
[Zhang, L ] C;Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Minist Forestry Bioethanol Res Ctr, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, State Key Lab Utilizat Woody Oil Resource, Changsha 410004, Peoples R China.
摘要：
The high-value utilization of Camellia oleifera, a major agroforestry waste, is critical for sustainable biomass management. This study presents a green integrated process for efficient lignin extraction and controllable nanoparticle synthesis via acidic solvent extraction and solvent-exchange nanotechnology. Two solvent systems were systematically optimized: HCl/1,4-dioxane achieved higher lignin purity (71.53%-73.52%) under optimal conditions (110 degrees C, 75 min, 90% solvent ratio), whereas p-TsOH/ethylene glycol low eutectic solvent (70 degrees C, 75 min, 65% ratio) yielded superior extraction efficiency (27.38%-28.74%). Subsequent solvent exchange enabled precise regulation of lignin nanoparticle morphology and size. Solvent polarity governed structural outcomes, with acetone producing elongated porous particles (153 +/- 1 nm, PDI = 0.322) and tetrahydrofuran (THF) generating uniform spheres (140 +/- 1 nm, PDI = 0.109). FTIR and zeta potential analyses revealed that tetrahydrofuran's hydrophobic effects enhanced surface electronegativity (-37.5 mV), conferring exceptional colloidal stability (<1% size increase over 30 days). Synergistic optimization of THF/water ratio (50%) and lignin concentration (0.7 mg/ml) produced ultrasmall nanoparticles (80 nm, PDI = 0.082). This work elucidates the multiscale mechanism of solvent polarity in lignin extraction-nanostructuring and establishes a low-carbon pathway for agroforestry waste valorization. The methodology demonstrates significant potential for advancing green material synthesis and nanotechnology applications through biomass-derived functional nanomaterials. (c) 2025 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International (CC BY-NC-ND) license (https://creativecommons.org/licenses/by-nc-nd/4.0/).
摘要：
Potassium (K) plays a pivotal role in influencing the structure and function of soil microbial communities, thereby influencing soil multifunctionality. Researches on various fertilization practices for Phoebe bournei has primarily focused on microbial communities. However, the mechanism of functional potential of microbe in mediating the influence of K on soil multifunctionality remains insufficiently elucidated. Here, the experiment included five K additions (CK, 0 g; K1, 60 g; K2, 120 g; K3, 180 g; and K4, 240 g per plant) in P. bournei young plantations via 16S rRNA sequencing and quantitative microbial element cycling (QMEC) smart chip technology to investigate the impacts of K additions on rhizosphere soil bacterial community attributes, nutrient cycling genes (carbon, nitrogen, phosphorus), and soil multifunctionality. K additions decreased bacterial diversity, while enhancing the abundance of genes involved in C degradation, including those related to labile and recalcitrant C, as well as N cycling, P cycling, and soil multifunctionality. Comparatively, K1 and K2 additions had slight effects on soil multifunctionality, bacterial communities and the abundance of C, N and P cycling genes. PLS-PM results demonstrated that K additions improve soil multifunctionality indirectly by altering bacterial community structure and network complexity, as well as the functional potential linked to N and P cycling. Additionally, soil abiotic factors are the was the core predictor for maintaining soil multifunctionality. All in all, soil properties and bacterial functional attributes together drive soil multifunctionality in response to K additions. These findings highlight that adequate K fertilizer may maintain soil multifunctionality, and regulate nutrient cycling and bacterial functions in P. bournei young plantations.
Potassium (K) plays a pivotal role in influencing the structure and function of soil microbial communities, thereby influencing soil multifunctionality. Researches on various fertilization practices for Phoebe bournei has primarily focused on microbial communities. However, the mechanism of functional potential of microbe in mediating the influence of K on soil multifunctionality remains insufficiently elucidated. Here, the experiment included five K additions (CK, 0 g; K1, 60 g; K2, 120 g; K3, 180 g; and K4, 240 g per plant) in P. bournei young plantations via 16S rRNA sequencing and quantitative microbial element cycling (QMEC) smart chip technology to investigate the impacts of K additions on rhizosphere soil bacterial community attributes, nutrient cycling genes (carbon, nitrogen, phosphorus), and soil multifunctionality. K additions decreased bacterial diversity, while enhancing the abundance of genes involved in C degradation, including those related to labile and recalcitrant C, as well as N cycling, P cycling, and soil multifunctionality. Comparatively, K1 and K2 additions had slight effects on soil multifunctionality, bacterial communities and the abundance of C, N and P cycling genes. PLS-PM results demonstrated that K additions improve soil multifunctionality indirectly by altering bacterial community structure and network complexity, as well as the functional potential linked to N and P cycling. Additionally, soil abiotic factors are the was the core predictor for maintaining soil multifunctionality. All in all, soil properties and bacterial functional attributes together drive soil multifunctionality in response to K additions. These findings highlight that adequate K fertilizer may maintain soil multifunctionality, and regulate nutrient cycling and bacterial functions in P. bournei young plantations.
摘要：
Conventional adsorbents for Hg(II) are not suitable for acidic environments because they degrade and lose activity. In this study, high-density sulfur and nitrogen-containing porous polyacrylonitrile sheet (HSN-PANS) was developed by grafting L-cysteine. The amphoteric HSN-PANS adsorbent can effectively remove Hg(II) from acidic wastewater, reducing its concentration from 11 mg/L to below the drinking water standard of 1 μg/L at pH 2. It shows high adsorption capacity (764 mg/g), strong anti-interference properties, and excellent selectivity. The adsorption mechanism involves coordination and electrostatic interactions. Thanks to its stable structure and efficient desorption, HSN-PANS demonstrates excellent acid resistance and reusability, retaining 99.9% adsorption efficiency after 43 cycles. Furthermore, it performs reliably in industrial wastewater treatment. Notably, an HSN-PANS-packed column can treat about 5,845 bed volumes (69 L) of Hg(II)-spiked acidic wastewater until reaching a breakthrough point of 1 μg/L, concentrating the adsorbed Hg(II) into 1.8 L of desorbent. This study demonstrates the potential of HSN-PANS as an effective adsorbent for directly and efficiently removing Hg(II) from acidic wastewater, providing a promising solution to reduce Hg(II) emissions in industrial processes.
Conventional adsorbents for Hg(II) are not suitable for acidic environments because they degrade and lose activity. In this study, high-density sulfur and nitrogen-containing porous polyacrylonitrile sheet (HSN-PANS) was developed by grafting L-cysteine. The amphoteric HSN-PANS adsorbent can effectively remove Hg(II) from acidic wastewater, reducing its concentration from 11 mg/L to below the drinking water standard of 1 μg/L at pH 2. It shows high adsorption capacity (764 mg/g), strong anti-interference properties, and excellent selectivity. The adsorption mechanism involves coordination and electrostatic interactions. Thanks to its stable structure and efficient desorption, HSN-PANS demonstrates excellent acid resistance and reusability, retaining 99.9% adsorption efficiency after 43 cycles. Furthermore, it performs reliably in industrial wastewater treatment. Notably, an HSN-PANS-packed column can treat about 5,845 bed volumes (69 L) of Hg(II)-spiked acidic wastewater until reaching a breakthrough point of 1 μg/L, concentrating the adsorbed Hg(II) into 1.8 L of desorbent. This study demonstrates the potential of HSN-PANS as an effective adsorbent for directly and efficiently removing Hg(II) from acidic wastewater, providing a promising solution to reduce Hg(II) emissions in industrial processes.
关键词：
Tree diversity;Soil organic matter decomposition;Temperature sensitivity;Rhizosphere;Microbial ecological strategy
摘要：
Background and aimsTree diversity strongly regulates organic matter inputs by rhizodeposition for microorganisms and microbial communities, impacting soil carbon (C) dynamics and stability. Because of much larger organic C availability in the rhizosphere, it can respond differently to tree diversity compared to bulk soil. To explore soil C stability under global warming, we assessed the temperature sensitivity (Q10) of organic matter decomposition in rhizosphere and bulk soil depending on tree diversity.MethodsQ10 of organic matter decomposition in rhizosphere and bulk soil in a subtropical forest were examined using short-term incubation under controlled conditions depending on tree diversity. Fine root traits and soil C and N availability were evaluated as related to microbial properties.ResultsWith increasing tree diversity, Q10 remained stable in the rhizosphere but decreased in the bulk soil. While greater tree diversity increased fine root biomass, soil C and N availability, microbial K/r strategy ratios in rhizosphere and bulk soil shifter towards the r strategists, with a reduced bacterial K/r strategy ratio. However, microbial gene copy numbers and Shannon diversity remained stable. Partial correlation and multiple regression analysis revealed that rhizosphere Q10 remained stable because of C excess and larger microbial abundance. The Q10 reduction in bulk soil correlated with increased C availability and a shift in microbial community towards a lower K/r strategy ratio.ConclusionThe Q10 decoupling between rhizosphere and bulk soil highlights a trade-off, where increasing tree diversity accelerates organic matter decomposition in rhizosphere to sustain nutrient supply, while maintaining bulk C pool stability under global warming.
摘要：
Cellulolytic enzymes for bioconversion of lignocellulose to fermentable sugar provide an economically viable solution for numerous biofuels production, but currently problematic due to the high cost of commercial cellulase. It has been assessed the secretome of full cellulase and hemicellulase, as well as auxiliary enzyme activity, were assessed in 17 fungal strains. The breakdown of pretreated poplar with various crude enzymes was investigated, and the synergistic effects of crude enzymes and commercial cellulase were evaluated. Correlation coefficients between different enzyme activities and glucose hydrolysis from pretreated poplar were also discussed. It was found cellobiohydrolase and xylanase activity were positively correlated with glucose release, but β-glucosidase activity was indeterminate and lysis polysaccharide monooxygenase activity had a poor correlation. The highest cellulose conversion rates from the pretreated poplar utilized commercial cellulase LLC02 combined with Trichoderma reesei ATCC 24449, Aspergillus niger SM24 and Aspergillus niger SM751 were 94.8 %, 91.6 %, and 103.6 %, respectively. These findings demonstrate the fact that coupling an appropriate amount of secretome cellulolytic enzyme cocktail with cellulase boosts woody biomass hydrolysis, implying that crude enzyme cocktails from selected fungal strains have a high potential for minimizing commercial cellulase costs for industrial applications.
Cellulolytic enzymes for bioconversion of lignocellulose to fermentable sugar provide an economically viable solution for numerous biofuels production, but currently problematic due to the high cost of commercial cellulase. It has been assessed the secretome of full cellulase and hemicellulase, as well as auxiliary enzyme activity, were assessed in 17 fungal strains. The breakdown of pretreated poplar with various crude enzymes was investigated, and the synergistic effects of crude enzymes and commercial cellulase were evaluated. Correlation coefficients between different enzyme activities and glucose hydrolysis from pretreated poplar were also discussed. It was found cellobiohydrolase and xylanase activity were positively correlated with glucose release, but β-glucosidase activity was indeterminate and lysis polysaccharide monooxygenase activity had a poor correlation. The highest cellulose conversion rates from the pretreated poplar utilized commercial cellulase LLC02 combined with Trichoderma reesei ATCC 24449, Aspergillus niger SM24 and Aspergillus niger SM751 were 94.8 %, 91.6 %, and 103.6 %, respectively. These findings demonstrate the fact that coupling an appropriate amount of secretome cellulolytic enzyme cocktail with cellulase boosts woody biomass hydrolysis, implying that crude enzyme cocktails from selected fungal strains have a high potential for minimizing commercial cellulase costs for industrial applications.
关键词：
Deep removal;Smelting wastewater;Adsorption;Rich pyrrolic-nitrogen-carbon;Tl(I)
摘要：
Acute toxicity from thallium (Tl) contamination poses significant risks to ecosystems and human health. Purifying Tl(I)-containing smelting wastewater is challenging due to the high mobility of Tl(I) and the complexity of such wastewater. As a soft acid, Tl(I) preferentially interacts with soft bases based on soft-hard interaction principles. In this study, we developed a pyrrolic-nitrogen-carbon sponge (NCS) as a model adsorbent for rapidly removing Tl(I) from complex aqueous environments, leveraging pyrrolic-N as a distinct soft base. The three-dimensional porous architecture of NCS facilitates quick diffusion of Tl(I) to adsorption sites. The optimized NCS-600 adsorbent can purify Tl(I)-contaminated water to drinking standards (< 0.1 µg/L) in just 10 min (at 0.4 g/L, C Tl(I) = 100 µg/L), reaching a high adsorption capacity of 252.14 mg/g. Mechanistic analysis showed that pyrrolic-N primarily facilitated Tl(I) adsorption, while the − OH group played a secondary role due to its minor steric hindrance effect. Outstanding performance was observed across wide pH (4–13) and temperature (15–35 °C) ranges, with negligible impacts of competing ions or coexisting organic compounds. NCS-600 reduced 60.806 µg/L of Tl(I) in zinc smelting wastewater to 0.069 µg/L within 30 min and completely removed 8.3 µg/L from natural water in only 3 min. It stably performed over at least 25 cycles. In fixed-bed operation, only 1.5 g of NCS-600 could purify and exceptionally large 10,109-bed volume (262 L) of contaminated water. This study provides a practical method for thorough decontamination of Tl(I)-containing smelting wastewater and offers new insights into designing advanced adsorbents for removing various heavy metals.
Acute toxicity from thallium (Tl) contamination poses significant risks to ecosystems and human health. Purifying Tl(I)-containing smelting wastewater is challenging due to the high mobility of Tl(I) and the complexity of such wastewater. As a soft acid, Tl(I) preferentially interacts with soft bases based on soft-hard interaction principles. In this study, we developed a pyrrolic-nitrogen-carbon sponge (NCS) as a model adsorbent for rapidly removing Tl(I) from complex aqueous environments, leveraging pyrrolic-N as a distinct soft base. The three-dimensional porous architecture of NCS facilitates quick diffusion of Tl(I) to adsorption sites. The optimized NCS-600 adsorbent can purify Tl(I)-contaminated water to drinking standards (< 0.1 µg/L) in just 10 min (at 0.4 g/L, C Tl(I) = 100 µg/L), reaching a high adsorption capacity of 252.14 mg/g. Mechanistic analysis showed that pyrrolic-N primarily facilitated Tl(I) adsorption, while the − OH group played a secondary role due to its minor steric hindrance effect. Outstanding performance was observed across wide pH (4–13) and temperature (15–35 °C) ranges, with negligible impacts of competing ions or coexisting organic compounds. NCS-600 reduced 60.806 µg/L of Tl(I) in zinc smelting wastewater to 0.069 µg/L within 30 min and completely removed 8.3 µg/L from natural water in only 3 min. It stably performed over at least 25 cycles. In fixed-bed operation, only 1.5 g of NCS-600 could purify and exceptionally large 10,109-bed volume (262 L) of contaminated water. This study provides a practical method for thorough decontamination of Tl(I)-containing smelting wastewater and offers new insights into designing advanced adsorbents for removing various heavy metals.
作者机构：
[Shi, Muling; Hong, Chao; Shi, ML] Hainan Univ, Sch Mat Sci & Engn, State Key Lab Trop Ocean Engn Mat & Mat Evaluat, Key Lab Electron Microscopy Hainan Prov P, Haikou 570228, Hainan, Peoples R China.;[Shi, Muling; Shi, ML] Hunan Univ, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Wang, Sixian; Yang, Yiqing; Pu, Zhangjie] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Shi, ML ] H;Hainan Univ, Sch Mat Sci & Engn, State Key Lab Trop Ocean Engn Mat & Mat Evaluat, Key Lab Electron Microscopy Hainan Prov P, Haikou 570228, Hainan, Peoples R China.;Hunan Univ, Mol Sci & Biomed Lab, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.
摘要：
Modern research in nutrition science is transitioning from classical methodologies to advanced analytical strategies, in which Raman spectroscopy plays a crucial role. Raman spectroscopy and its derived techniques are gaining recognition in nutrition science for their features, such as high-speed, non-destructive analysis, label-free multiple detection and high sensitivity. Raman-enhancing techniques have further improved the sensitivity of Raman spectroscopy and widely extended its detection and imaging applications in nutrient analysis, as well as in ancillary tasks for nutrition research, such as nutrient status evaluation, nutrient interaction and metabolism studies. Further development of Raman-based analytical approaches lies in the improvement of instruments with higher precision, as well as the incorporation of other analytical techniques and advanced data analysis tools. This paper provides a comprehensive review of the application of nanoscience and nanotechnology, with a specific focus on Raman technology, in the field of food and nutrition science research. Instead of delving into the quantitative or qualitative detection capabilities of Raman technology, we highlight the remarkable food analysis and nutrition research methods established by this technology. Generally, this review introduces the characteristics and applications of Raman technology in nutrition analysis and discusses the limitations and future prospects of Raman spectroscopy for nutrition monitoring.
作者：
Na Liu*;Mingkai Wang;Yijun Zhou;Lishu Shao;Lin Zhang;...
期刊：
Green Carbon,2025年 ISSN：2950-1555
通讯作者：
Na Liu
作者机构：
[Mingkai Wang; Yijun Zhou] School of Chemistry and Chemical Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;Ministry of Forestry Bioethanol Research Center, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[Na Liu; Lishu Shao; Lin Zhang; Peng Zhan; Zhiping Wu] School of Chemistry and Chemical Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China<&wdkj&>Ministry of Forestry Bioethanol Research Center, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
通讯机构：
[Na Liu] S;School of Chemistry and Chemical Engineering, Central South University of Forestry and Technology, Changsha, Hunan 410004, China<&wdkj&>Ministry of Forestry Bioethanol Research Center, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
摘要：
1,3-Butadiene is an important chemical raw material, especially with its large market demand as the main monomer for rubber tires. It is mainly produced by the thermal cracking of naphtha as a by-product of olefin production. The production process faces dual pressures of petroleum resource consumption and environmental issues. Ethanol, a renewable resource, offers a strategy for green, low-carbon, economical, and sustainable production of butadiene under the "dual carbon" context. This review summarizes the reaction mechanisms, catalyst types, recent research progress, and existing challenges related to the direct conversion of ethanol to butadiene (ETB). It focuses specifically on how water affects catalysts during the direct conversion of aqueous ethanol to butadiene (AETB), offering fresh perspectives and methods for creating effective and hydrophobic catalysts.
1,3-Butadiene is an important chemical raw material, especially with its large market demand as the main monomer for rubber tires. It is mainly produced by the thermal cracking of naphtha as a by-product of olefin production. The production process faces dual pressures of petroleum resource consumption and environmental issues. Ethanol, a renewable resource, offers a strategy for green, low-carbon, economical, and sustainable production of butadiene under the "dual carbon" context. This review summarizes the reaction mechanisms, catalyst types, recent research progress, and existing challenges related to the direct conversion of ethanol to butadiene (ETB). It focuses specifically on how water affects catalysts during the direct conversion of aqueous ethanol to butadiene (AETB), offering fresh perspectives and methods for creating effective and hydrophobic catalysts.
关键词：
climate change;conservation;giant panda;golden snub-nosed monkey;reserve network;umbrella species
摘要：
Umbrella species are widely used as conservation strategies for the design of reserves. However, empirical data on their potential effectiveness, particularly in the context of climate change, is limited and inconclusive. Here, we evaluated the potential umbrella effectiveness of the giant panda (Ailuropoda melanoleuca) and its reserve network in the Qinling Mountains for the conservation of sympatric golden snub-nosed monkeys (Rhinopithecus roxellana) under climate change. We modeled their current and future habitat suitability index (HSI) by using the MaxEnt model and analyzed their current and future spatial congruence of HSI. Their suitable areas were also overlaid onto the boundaries and management zones of the reserve network. Subsequently, we designed a series of indices to assess the potential umbrella effectiveness of the reserve network under climate change. Our results indicate that their habitat spatial congruence will remain high in the future. The suitable habitat of giant pandas overlaps substantially with that of golden snub-nosed monkeys in both present and future. Importantly, the umbrella effectiveness of the reserve network for the golden snub-nosed monkey will not decrease. However, there are still some protection gaps shared by them outside the reserve network. Overall, our results demonstrate that the giant panda and its reserve network can serve as an effective umbrella for the golden snub-nosed monkey under climate change, providing theoretical support to the application of umbrella species under climate change.
作者机构：
College of Life and Environmental Sciences, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha 410004, Hunan, China;Key Laboratory of Photoelectric Conversion and Utilization of Solar Energy, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, Shandong, China;Shandong Energy Institute, Qingdao 266101, Shandong, China;Qingdao New Energy Shandong Laboratory, Qingdao 266101, Shandong, China
通讯机构：
[Shaoming Mao] C;College of Life and Environmental Sciences, Central South University of Forestry and Technology, Changsha 410004, Hunan, China<&wdkj&>Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry & Technology, Changsha 410004, Hunan, China
摘要：
Cyanobacteria, ancient photosynthetic prokaryotes capable of directly converting CO₂ and solar energy into various chemicals through photosynthesis, have drawn great attention as a photosynthetic biomanufacturing platform. To enhance the production of various chemicals, multi-dimensional engineering strategies spanning genetic modification to process optimization have been developed. Notably, stress activation strategies, characterized by their strong controllability and the absence of transgenic risks, have recently attracted significant attention. These strategies could reprogram native metabolic networks, effectively redirecting photosynthetic flux toward targeted biosynthesis pathways while maintaining cellular viability, thereby boosting the production of both bulk and value-added chemicals. This review summarizes recent advances in stress-driven cyanobacterial chemical production, including compatible solutes, pigments and biofuels. We dissect regulatory mechanisms at transcriptional, translational, and enzymatic levels, and discuss challenges and potential strategies for enhancing the large-scale industrial production of cyanobacteria under stress conditions.
Cyanobacteria, ancient photosynthetic prokaryotes capable of directly converting CO₂ and solar energy into various chemicals through photosynthesis, have drawn great attention as a photosynthetic biomanufacturing platform. To enhance the production of various chemicals, multi-dimensional engineering strategies spanning genetic modification to process optimization have been developed. Notably, stress activation strategies, characterized by their strong controllability and the absence of transgenic risks, have recently attracted significant attention. These strategies could reprogram native metabolic networks, effectively redirecting photosynthetic flux toward targeted biosynthesis pathways while maintaining cellular viability, thereby boosting the production of both bulk and value-added chemicals. This review summarizes recent advances in stress-driven cyanobacterial chemical production, including compatible solutes, pigments and biofuels. We dissect regulatory mechanisms at transcriptional, translational, and enzymatic levels, and discuss challenges and potential strategies for enhancing the large-scale industrial production of cyanobacteria under stress conditions.
期刊：
Science of The Total Environment,2025年958:178115 ISSN：0048-9697
通讯作者：
Weili Jia<&wdkj&>Chuanxin Ma
作者机构：
[Xu, Xinxin; Hao, Yi; Cai, Zeyu] Guangdong Basic Research Center of Excellence for Ecological Security and Green Development, Key Laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education, School of Ecology, Environmental and Resources, Guangdong University of Technology, Guangzhou 510006, China;[Cao, Yini] Faculty of Life Science and Technology, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[Jia, Weili] SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, School of Environment, South China Normal University, Guangzhou 510006, China. Electronic address: weili.jia@m.scnu.edu.cn;[Zhao, Jian] Institute of Coastal Environmental Pollution Control, Key Laboratory of Marine Environment and Ecology (Ministry of Education), Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, China;[White, Jason C] The Connecticut Agricultural Experiment Station, New Haven, CT 06504, United States
通讯机构：
[Weili Jia] S;[Chuanxin Ma] G;SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Theoretical Chemistry of Environment, School of Environment, South China Normal University, Guangzhou 510006, China<&wdkj&>Guangdong Basic Research Center of Excellence for Ecological Security and Green Development, Key Laboratory for City Cluster Environmental Safety and Green Development of the Ministry of Education, School of Ecology, Environmental and Resources, Guangdong University of Technology, Guangzhou 510006, China
摘要：
Boron (B) deficiency affects over 132 crop species globally, making effective B supplement crucial for enhancing agricultural yield and health. This study explores an innovative application of nanoscale boron nitride (nano-BN) as a sustainable solution for addressing B deficiency in crops. Cucumber seedlings were treated with different contents of nano-BN under greenhouse conditions and both B and N ionic treatments were set as comparisons. Results show that soil application of 10 mg/kg nano-BN achieved a remarkable 15.8 % increase in fresh weight compared to the control. Notably, nano-BN exhibited superior efficiency in providing essential micronutrients without inducing toxicity as compared to traditional ionic B sources. Phytohormone correlation analysis reveals that nano-BN application significantly enhances levels of indole-3-acetic acid (IAA) and cytokinins while reducing abscisic acid (ABA), fostering optimal plant growth conditions. Furthermore, increases in dissolved organic matter (DOM) and dissolved organic carbon (DOC) levels in the rhizosphere improve nutrient availability and promote beneficial microbial activity in the soil as affected by nano-BN. Metagenomics techniques were used to investigate the impact of nano-BN on soil carbon and nitrogen cycling, alongside its effects on the soil microbiome. The upregulation of genes associated with fermentation pathways as affected by nano-BN suggests the enhanced carbon cycling. Additionally, nano-BN upregulated a number of functional genes involved in nitrogen-based processes, leading to a significant increase in microorganisms harboring nitrogen-fixing genes, including Phenylobacterium, Novosphingobium, and Reyranella. Overall, these findings provide valuable insight into the application of nano-BN in agriculture to sustainably increase crop productivity and enhance the efficiency of carbon and nitrogen cycling.
Boron (B) deficiency affects over 132 crop species globally, making effective B supplement crucial for enhancing agricultural yield and health. This study explores an innovative application of nanoscale boron nitride (nano-BN) as a sustainable solution for addressing B deficiency in crops. Cucumber seedlings were treated with different contents of nano-BN under greenhouse conditions and both B and N ionic treatments were set as comparisons. Results show that soil application of 10 mg/kg nano-BN achieved a remarkable 15.8 % increase in fresh weight compared to the control. Notably, nano-BN exhibited superior efficiency in providing essential micronutrients without inducing toxicity as compared to traditional ionic B sources. Phytohormone correlation analysis reveals that nano-BN application significantly enhances levels of indole-3-acetic acid (IAA) and cytokinins while reducing abscisic acid (ABA), fostering optimal plant growth conditions. Furthermore, increases in dissolved organic matter (DOM) and dissolved organic carbon (DOC) levels in the rhizosphere improve nutrient availability and promote beneficial microbial activity in the soil as affected by nano-BN. Metagenomics techniques were used to investigate the impact of nano-BN on soil carbon and nitrogen cycling, alongside its effects on the soil microbiome. The upregulation of genes associated with fermentation pathways as affected by nano-BN suggests the enhanced carbon cycling. Additionally, nano-BN upregulated a number of functional genes involved in nitrogen-based processes, leading to a significant increase in microorganisms harboring nitrogen-fixing genes, including Phenylobacterium, Novosphingobium, and Reyranella. Overall, these findings provide valuable insight into the application of nano-BN in agriculture to sustainably increase crop productivity and enhance the efficiency of carbon and nitrogen cycling.
摘要：
Oxidative stress, resulting from the overproduction of reactive oxygen species (ROS), represents a significant risk factor for the development of pathological conditions, including liver injury and brain injury (characterized by neurological damage). There is a compelling requirement to investigate more convenient and efficacious drug delivery strategies for the treatment of hepatic and neurological injuries. In this study, near-infrared threonine-quercetin carbon dots (TQ CDs) with antioxidant activity were synthesized. The bioimaging results demonstrated that the TQ CDs were capable of aggregating in both the liver and the brain via both intravenous and gavage administration. Furthermore, we present a straightforward methodology for investigating the relationship between the concentration of intravenous administration and the concentration of gavage administration. Our findings indicate that the intravenous bioimaging effect and biological effect of intravenous administration could be achieved by increasing the concentration of the TQ CDs administered via gavage. Also, the biological effects including protection of the liver and brain were corroborated through in vivo liver injury and neurological injury models. Overall, this study opens up an explorable field of nanoparticle-based oral drug delivery strategies for bioimaging, organ protection, and treating liver and brain diseases.
Oxidative stress, resulting from the overproduction of reactive oxygen species (ROS), represents a significant risk factor for the development of pathological conditions, including liver injury and brain injury (characterized by neurological damage). There is a compelling requirement to investigate more convenient and efficacious drug delivery strategies for the treatment of hepatic and neurological injuries. In this study, near-infrared threonine-quercetin carbon dots (TQ CDs) with antioxidant activity were synthesized. The bioimaging results demonstrated that the TQ CDs were capable of aggregating in both the liver and the brain via both intravenous and gavage administration. Furthermore, we present a straightforward methodology for investigating the relationship between the concentration of intravenous administration and the concentration of gavage administration. Our findings indicate that the intravenous bioimaging effect and biological effect of intravenous administration could be achieved by increasing the concentration of the TQ CDs administered via gavage. Also, the biological effects including protection of the liver and brain were corroborated through in vivo liver injury and neurological injury models. Overall, this study opens up an explorable field of nanoparticle-based oral drug delivery strategies for bioimaging, organ protection, and treating liver and brain diseases.
摘要：
The urbanization process is complex and lengthy, typically resulting in dual changes in the socioeconomic structure and ecological environment. However, in the context of arid environments and initial urbanization, emerging towns undergo evolutionary processes different from those of traditional cities. This study focuses on a typical town, analyzing its growth under the combined effects of arid conditions and incipient urbanization. The results reveal a unique urbanization trajectory in the hinterland of southern Xinjiang: transitioning from refined agricultural planting to shrubland and bare land dominated by natural factors and then to impervious surfaces. While the pattern was complex, the direction of transition was clear. Using the town's establishment in 2014 as a critical node for urbanization initiation, shrubland emerged as the most sensitive land type, with a proportional increase by a factor of 2.6 from 2010 to 2015. This was driven by the abandonment of cultivated land, which decreased by 11.3% during the study period, with 78% of the newly added shrubland area converted from cropland. By applying the Markov model together with the InVEST model, the study predicted urban land-use transition patterns over the next 5years and revealed that urbanization primarily exacerbates the instability of water yield in the surrounding region. This study uniquely addresses the gap in understanding the impacts of the urbanization process of emerging towns in arid regions and its associated ecological processes. A detailed investigation of such urbanization is crucial to mitigating issues like disorderly land use and promoting the sustainable development of small and medium-sized towns.
作者机构：
[Guo, Hong; Lei, XD; Fu, Liyong; Lei, Xiangdong; He, Xiao; Gao, Wenqiang] Chinese Acad Forestry, State Forestry & Grassland Adm, Key Lab Forest Management & Growth Modelling, State Key Lab Efficient Prod Forest Resources,Inst, Beijing, Peoples R China.;[Liang, Maowei] Univ Minnesota, Cedar Creek Ecosyst Sci Reserve, East Bethel, MN USA.;[Xiang, Wenhua] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha, Peoples R China.;[Sharma, Ram P.] Tribhuvan Univ, Inst Forestry, Kathmandu, Nepal.;[Chen, Zhicheng] Chinese Acad Forestry, Natl Forestry & Grassland Adm, Ecol & Nat Conservat Inst, Key Lab Forest Ecol & Environm, Beijing, Peoples R China.
通讯机构：
[Lei, XD ] C;Chinese Acad Forestry, State Forestry & Grassland Adm, Key Lab Forest Management & Growth Modelling, State Key Lab Efficient Prod Forest Resources,Inst, Beijing, Peoples R China.
关键词：
community-weighted mean of traits;complementarity effect;functional diversity;mass-ratio effect;natural forest;species richness
摘要：
Increasing evidence shows that biodiversity–ecosystem functioning relationships (BEFs) become stronger as forests develop, but much of the evidence is drawn from experiments (less than 30 years). How the biodiversity effects vary with stand development stages remains largely unexplored. Using a large temperate forest dataset with 2392 permanent plots in northeastern China, we examined the relationships between biodiversity (i.e. tree species richness, functional diversity, and functional composition) and aboveground biomass (AGB) across different development stages of temperate forests (covering all stages from young to overmature forests). Specifically, the complementarity and mass‐ratio effects across different forest development stages were evaluated to elucidate emerging patterns that explain ecosystem functioning. We observed positive BEFs using both tree species richness and functional diversity, but these positive effects decreased with forest development. However, the effects of community‐weighted mean (CWM) on AGB showed two peaks in young and mature stands. Interestingly, the effects of CWM on AGB became larger than the effects of functional diversity after the forests developed to near‐mature/mature stands, indicating that BEFs are driven by mass‐ratio effects (i.e. dominant tree species) rather than niche complementarity in old stands. The high AGB in young stands was characterized by tree species with high resource acquisition ability, however, in old stands, it was associated with tree species with both high resource acquisition ability and conservative traits. Our findings indicate how the developmental stage influences the effects of biodiversity on ecosystem functioning in natural forests. The findings tentatively advocate for a mechanistic framework of BEFs covering all developmental stages of temperate forests, which could facilitate the formulation of effective strategies for enhancing ecosystem functioning at different development stages.
作者机构：
[Chen, Yi; Su, Qianqian; Zhang, Chen; Xiang, Zuofu; Liu, Yang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.;[Guo, Cheng; Yu, Yang; Chen, Yixin] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Changsha, Hunan, Peoples R China.;[Yue, Ling] Panzhihua Anim Dis Prevent & Control Ctr, Panzhihua, Sichuan, Peoples R China.
通讯机构：
[Su, QQ; Xiang, ZF ] C;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Hunan, Peoples R China.
摘要：
The unique environment of the Qinghai-Tibetan Plateau provides a great opportunity to study how primate intestinal microorganisms adapt to ecosystems. The 16S rRNA gene amplicon and metagenome analysis were conducted to investigate the correlation between gut microbiota in primates and other sympatric animal species living between 3600 and 4500 m asl. Results showed that within the same geographical environment, Macaca mulatta and Rhinopithecus bieti exhibited a gut microbiome composition similar to that of Tibetan people, influenced by genetic evolution of host, while significantly differing from other distantly related animals. The gut microbiota of plateau species has developed similar strategies to facilitate their hosts’ adaptation to specific environments, including broadening its dietary niche and enhancing energy absorption. These findings will enhance our comprehension of the significance of primate gut microbiota in adapting to specific habitats. Analyses of gut microbiomes from sympatric animals of the Qinghai-Tibetan Plateau and comparison with Tibetan human gut microbiomes reveals the impact of host phylogeny on gut microbiota composition.
摘要：
Based on both morphological and genetic data, we describe a new cryptic species of Calotes from southeastern Xizang Autonomous Region, China. The new species was confused with C. paulus, and it is morphologically most similar to members of the C. paulus complex (namely C. paulus, C. sinyik, and C. zolaiking), but the new species shows considerable genetic divergence from closely resembled congeners and can be diagnosed morphologically by having more vertebral crest scales, more scale rows at mid body, and more caudal vertebrae. With the description of the new species, we also remove C. paulus from the reptile fauna of China.
期刊：
Frontiers in Microbiology,2025年15:1499315 ISSN：1664-302X
作者机构：
[Li, Yue-Yuan; Ren, Ye-Song; Luo, Qing-Chen] Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Science and Technology, Central South University of Forestry and Technology, Changsha, China;[Yang, Xiao-Hui] College of Life Science, Hunan Normal University, Changsha, China;[Zhu, Dao-Hong] College of Forestry, Central South University of Forestry and Technology, Changsha, China
摘要：
INTRODUCTION: Phage WO represents the sole bacteriophage identified to infect Wolbachia, exerting a range of impacts on the ecological dynamics and evolutionary trajectories of its host. Given the extensive prevalence of Wolbachia across various species, phage WO is likely among the most prolific phage lineages within arthropod populations. To examine the diversity and evolutionary dynamics of phage WO, we conducted a screening for the presence of phage WO in Wolbachia-infected cricket species from China. METHODS: The presence of phage WO was detected using a PCR-based methodology. To elucidate the evolutionary forces driving phage WO diversity, analyses of intragenic recombination were conducted employing established recombination techniques, and horizontal transmission was investigated through comparative phylogenetic analysis of the phages and their hosts. RESULTS AND DISCUSSION: Out of 19 cricket species infected with Wolbachia, 18 species were found to harbor phage WO. Notably, 13 of these 18 cricket species hosted multiple phage types, with the number of types ranging from two to 10, while the remaining five species harbored a single phage type. Twelve horizontal transmission events of phage WO were identified, wherein common phage WO types were shared among different Wolbachia strains. Notably, each phage WO horizontal transfer event was associated with distinct Wolbachia supergroups, specifically supergroups A, B, and F. Previous studies have found that four Wolbachia strains infect two to five species of crickets. However, among these cricket species, in addition to the shared phage WO types, all harbored species-specific phage WO types. This suggests that Wolbachia in crickets may acquire phage WO types through horizontal viral transfer between eukaryotes, independent of Wolbachia involvement. Furthermore, nine putative recombination events were identified across seven cricket species harboring multiple phage types. These findings suggest that horizontal transmission and intragenic recombination have played a significant role in the evolution of the phage WO genome, effectively enhancing the diversity of phage WO associated with crickets.
通讯机构：
[Liu, GQ ; Liu, YN] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha, Peoples R China.;Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Res, Changsha, Peoples R China.;Lab Yuelushan Seed Ind, Changsha, Peoples R China.
摘要：
Treatment with C18:1 and C18:2, but not C18:0, increased the triterpenoid content of the medicinal fungus Sanghuangporus lonicericola. We identified 413 terpenoids, including 210 volatile terpenoids. Eight upregulated terpenoids, including 3,13,15-trihydroxyoleanane-12-one, dulcioic acid and serrat-14-ene-3,20,24,29-tetrol, were shared between the C18:1 and C18:2 treatments but not the C18:0 treatment. The C18:1 and C18:2 treatments increased the levels of 12 and 7 odour-related terpenoids, respectively, and increased the level of alpha-farnesene (herbal odour). Gene set enrichment analysis revealed that compared with C18:0, C18:1 and C18:2 produced stronger activation of the terpenoid biosynthesis, fatty acid degradation, and MAPK signalling pathways and stronger inhibition of basal transcription factors at both the transcript and protein levels. Finally, two-way orthogonal partial least squares analysis revealed that gene and protein expression in the identified pathways was correlated with levels of unsaturated fatty acid-induced terpenoid metabolites. Together, our integrated multiomics data revealed the key pathways involved in unsaturated fatty acid-induced terpenoid biosynthesis in S. lonicericola.
