Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade-Cas3 system

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成果类型：

期刊论文

作者：

Zheng, Wentao;Xia, Yandong;Wang, Xue;Gao, Shiqing;Zhou, Diao;...

通讯作者：

Fu, Jun;Li, RJ;Yin, J

作者机构：

[Zhang, Youming; Li, Ruijuan; Zheng, Wentao; Wang, Xue; Jiang, Chanjuan; Li, RJ; Fu, Jun; Fu, J; Gao, Shiqing; Tu, Qiang; Ravichandran, Vinothkannan] Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China.

[Zhou, Diao; Yin, Yulong; Xia, Yandong; Yin, Jia] Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.

[Xia, Yandong] Cent South Univ Forestry & Technol, Coll Life Sci & Technol, Key Lab Natl Forestry Grassland & Adm Control Arti, Hunan Prov Key Lab Control Forest Dis & Pests,Key, Changsha, Peoples R China.

[Zhang, Youming; Tu, Qiang] Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, CAS Key Lab Quantitat Engn Biol, Shenzhen, Peoples R China.

通讯机构：

[Yin, J ] H

[Li, RJ ; Fu, J] S

Shandong Univ, State Key Lab Microbial Technol, Qingdao, Peoples R China.

Hunan Normal Univ, Coll Life Sci, Hunan Prov Key Lab Anim Intestinal Funct & Regulat, Hunan Int Joint Lab Anim Intestinal Ecol & Hlth, Changsha 410081, Peoples R China.

语种：

英文

期刊：

Nature Protocols

ISSN：

1754-2189

年：

2023

卷：

期：

页码：

2642-2670

DOI：

10.1038/s41596-023-00856-1

基金类别：

National Key R&D Program of China [2019YFA0904000]; Natural Science Foundation of China [31570094, 31670097, 81502962]; The 111 Project [B16030]; China Postdoctoral Science Foundation [2022M711925]; Shandong Provincial Natural Science Foundation of China [ZR2020MC015, ZR2022QC107]; Guangdong Basic and Applied Basic Research Foundation [2022A1515110795]; Natural Science Foundation of Changsha [kq2208167]; Natural Science Foundation for Distinguished Young Scholars of Hunan Province [2023JJ10029]; Taishan Scholar Program of Shandong Province

机构署名：

本校为其他机构

院系归属：

林学院

生命科学与技术学院

摘要：

A lack of generic and effective genetic manipulation methods for Pseudomonas has restricted fundamental research and utilization of this genus for biotechnology applications. Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome engineering. This PEHR system is based on a lambda Red-like operon (BAS) from Pseudomonas aeruginosa phage Ab31 and a Rac bacteriophage RecET-like operon (Rec-TEPsy) from P. syringae pv. syringae B728a and also contains exogenous elements, including the RecBCD inhibitor (Redγ or Pluγ)...

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Precise genome engineering in Pseudomonas using phage-encoded homologous recombination and the Cascade-Cas3 system

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