期刊:
Enzyme and Microbial Technology,2024年178:110447 ISSN:0141-0229
通讯作者:
Zhou, Xiang;Lu, Dong
作者机构:
[Zhang, Miao-Miao; Wu, Qing-Feng; Zhou, Xiang; Lu, Dong; Jia, Cheng-Lin; Lei, Cai-Rong; Zhao, Jing-Ru; Ren, Jun-Le; Liu, Mei-Han; Wang, Ya-Juan; Ding, Nan; Dong, Zi-Yi] Institute of Modern Physics, Chinese Academy of Sciences,Lanzhou 730000,China;[Zhang, Miao-Miao; Zhou, Xiang; Lu, Dong; Jia, Cheng-Lin; Lei, Cai-Rong; Zhao, Jing-Ru; Ren, Jun-Le; Wang, Ya-Juan; Ding, Nan; Dong, Zi-Yi] University of Chinese Academy of Sciences, Chinese Academy of Science, Beijing 100049, China;[Zhou, Xiang] Kejin Innovation Institute of Heavy Ion Beam Biological Industry, Baiyin 730900, China. Electronic address: syannovich@impcas.ac.cn;[Zhang, Miao-Miao] Gansu Key Laboratory of Microbial Resources Exploitation and Application, Lanzhou 730070, China;[Zhou, Bo; Liu, Mei-Han; Liu, Jun] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410000, China
通讯机构:
[Lu, Dong] G;[Zhou, Xiang] K;Kejin Innovation Institute of Heavy Ion Beam Biological Industry, Baiyin 730900, China. Electronic address:;Gansu Key Laboratory of Microbial Resources Exploitation and Application, Lanzhou 730070, China. Electronic address:
关键词:
Carbon ion beam irradiation;Clostridium butyricum;Enzyme activity;Tolerance
摘要:
Clostridium butyricum (C. butyricum) represents a new generation of probiotics, which is beneficial because of its good tolerance and ability to produce beneficial metabolites, such as short-chain fatty acids and enzymes; however, its low enzyme activity limits its probiotic efficacy. In this study, a mutant strain, C. butyricum FZM 240 was obtained using carbon ion beam irradiation, which exhibited greatly improved enzyme production and tolerance. The highest filter paper, endoglucanase, and amylase activities produced by C. butyricum FZM 240 were 125.69 U/mL, 225.82 U/ mL, and 252.28 U/mL, which were 2.58, 1.95, and 2.21-fold higher, respectively, than those of the original strain. The survival rate of the strain increased by 11.40 % and 5.60 % after incubation at 90 °C for 5 min and with simulated gastric fluid at pH 2.5 for 2 h, respectively, compared with that of the original strain. Whole-genome resequencing and quantitative real-time PCR(qRT-PCR) analysis showed that the expression of genes related to enzyme synthesis (GE000348, GE001963 and GE003123) and tolerance (GE001114) was significantly up-regulated, while that of genes related to acid metabolism (GE003450) was significantly down-regulated. On this basis, homology modeling and functional prediction of the proteins encoded by the mutated genes were performed. According to the results, the properties related to the efficacy of C. butyricum as a probiotic were significantly enhanced by carbon ion beam irradiation, which is a novel strategy for the application of Clostridium spp. as feed additives.
期刊:
International Journal of Biological Macromolecules,2024年264(Pt 1):130563 ISSN:0141-8130
通讯作者:
Xu, Dong
作者机构:
[Luo, Shan; Peng, Chenzhan; Luo, Hongmei; Xu, Dong; Long, Tiantian; Ouyang, Min; Lin, Qinlu] National Engineering Laboratory for Rice and By-products Further Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha, 410004, China;[Luo, Shan; Peng, Chenzhan; Luo, Hongmei; Long, Tiantian; Ouyang, Min] Hunan Province Key Laboratory of Edible forestry Resources Safety and Processing Utilization, Central South University of Forestry and Technology, Changsha, 410004, China;[Xu, Dong] Hunan Province Key Laboratory of Edible forestry Resources Safety and Processing Utilization, Central South University of Forestry and Technology, Changsha, 410004, China. Electronic address: philip198349@gmail.com
通讯机构:
[Dong Xu] N;National Engineering Laboratory for Rice and By-products Further Processing, College of Food Science and Engineering, Central South University of Forestry & Technology, Changsha, 410004, China<&wdkj&>Hunan Province Key Laboratory of Edible forestry Resources Safety and Processing Utilization, Central South University of Forestry and Technology, Changsha, 410004, China
摘要:
Both sensing and removal of Hg(II) are important to environment and human health in view of the high toxicity and wide applications of mercury in industry. This study aims to develop a cellulose-based fluorescent aerogel for simultaneous Hg(II) sensing and removal via conveniently cross-linking two nanomaterials cellulose nanocrystals and bovine serum albumin-functionalized gold nanoclusters (BSA-AuNCs) with epichlorohydrin. The aerogel exhibited strong homogeneous red fluorescence at the non-edged regions under UV light due to highly dispersed BSA-AuNCs in it, and its fluorescence could be quenched by Hg(II). Through taking pictures with a smartphone, Hg(II) in the range of 0-1000μg/L could be quantified with a detection limit of 12.7μg/L. The sorption isotherm of Hg(II) by the aerogel followed Freundlich modelwith an equation of Q(e)=0.329*C(e)(1/0.971)and a coefficient of 0.999. The maximum sorption capacity can achieve 483.21mg/g for Hg(II), much higher than many reported sorbents. The results further confirmed Hg(II) strong sorption and sensitive detection are due to its complexation and redox reaction with the chemical groups in aerogels and its strong fluorescence quenching effect. Due to extensive sources and low cost, cellulose is potential to be developed into aerogels with multiple functions for sophisticated applications.
摘要:
Alcoholic liver disease (ALD) is primarily caused by long-term excessive alcohol consumption. Cyanidin-3-O-glucoside (C3G) is a widely occurring natural anthocyanin with multiple biological activities. This study aims to investigate the effects of C3G isolated from black rice on ALD and explore the potential mechanism. C57BL/6J mice (male) were fed with standard diet (CON) and Lieber-DeCarli liquid-fed (Eth) or supplemented with a 100 mg/kg/d C3G Diet (Eth-C3G), respectively. Our results showed that C3G could effectively ameliorate the pathological structure and liver function, and also inhibited the accumulation of liver lipids. C3G supplementation could partially alleviate the injury of intestinal barrier in the alcohol-induced mice. C3G supplementation could increase the abundance of Norank_f_Muribaculaceae, meanwhile, the abundances of Bacteroides, Blautia, Collinsella, Escherichia-Shigella, Enterococcus, Prevotella, [Ruminococcus]_gnavus_group, Methylobacterium-Methylorubrum, Romboutsia, Streptococcus, Bilophila, were decreased. Spearman's correlation analysis showed that 12 distinct genera were correlated with blood lipid levels. Non-targeted metabolic analyses of cecal contents showed that C3G supplementation could affect the composition of intestinal metabolites, particularly bile acids. In conclusion, C3G can attenuate alcohol-induced liver injury by modulating the gut microbiota and metabolites, suggesting its potential as a functional food ingredient against alcoholic liver disease.
作者:
Awudi, Doris Abra;Walker, Anita Nyarkoa*;Weeto, Mary Makhala;Priddy, Christiana Babymay;Akan, Otobong Donald;...
期刊:
FRONTIERS IN NUTRITION,2024年11:1291360 ISSN:2296-861X
通讯作者:
Walker, Anita Nyarkoa;Feng, Q
作者机构:
[Walker, Anita Nyarkoa; Priddy, Christiana Babymay; Walker, AN; Feng, Q; Feng, Qing; Zhang, Yuandie; Awudi, Doris Abra; Feng, Yucong; Zaitoun, Margaret; Wei, Tao; Zhong, Yuxia; Weeto, Mary Makhala] Nanjing Med Univ, Dept Nutr & Food Hyg, Nanjing, Jiangsu, Peoples R China.;[Akan, Otobong Donald] Akwa Ibom State Univ, Microbiol Dept, Uyo, Akwa Ibom State, Nigeria.;[Akan, Otobong Donald] Cent South Univ Forestry & Technol, Food Sci & Engn, Changsha, Hunan, Peoples R China.;[Baduweh, Cynthia Anuseh] Nanjing Med Univ, Sch Publ Hlth, Nanjing, Jiangsu, Peoples R China.;[Arthur, Bella Abigail] Jiangsu Univ, Sch Med, Zhenjiang, Jiangsu, Peoples R China.
通讯机构:
[Walker, AN; Feng, Q ] N;Nanjing Med Univ, Dept Nutr & Food Hyg, Nanjing, Jiangsu, Peoples R China.
关键词:
Africans;China;diet quality;eating habits;global diet quality score;overweight or obesity;students;western diet
摘要:
BACKGROUND: The impact of non-communicable diseases (NCDs) is disproportionately felt by immigrants from low- to medium-income countries (LMICs), partly due to their dietary habits. To thrive in their new environment, migrants either omit or consume certain food items, which could lead to nutritional deficits. As a result, most migrants experience more NCDs than their compatriots in their native countries. Therefore, we evaluated the difference in dietary habits, quality, and the influencing factors of overweight or obesity among African migrant students in Nanjing (China) and non-migrant students in Africa using cross-sectional data. METHODS: The researchers used the food frequency questionnaire and the global diet quality score metrics to assess food intake and quality, respectively. Then, cross-tabulation was employed to explore the differences between the groups in meal skipping, eating habits, and diet quality. Finally, the factors associated with overweight or obesity were assessed with binary logistic regression stratified by African students in Nanjing and students in their native countries. RESULTS: Approximately 678 responses were received, mainly between 18-25 years (46.7%) and 26-36 years (45.4 %). The majority of them (52.3%) were international students. The non-migrant African students' diets lacked citrus fruits (22.2%), deep orange fruits (15.4%), deep orange vegetables (18%), cruciferous vegetables (24.6%), and dark leafy vegetables (26.5%). While the African migrant students consumed more high-fat dairy (50.7%), processed meats (23.9%), sweets and ice creams (51.3%), sugar-sweetened beverages (40.5%), and juice (61.5%), p < 0.001. Furthermore, consuming late-night meals constantly [Exp (B) = 39.607, p = 0.049], eating twice a day [Exp (B) = 6.527, p = 0.036], consuming red meat [Exp (B) = 29.287, p = 0.001], processed meats [Exp (B) = 719.979, p = 0.0011], refined grains and baked foods [Exp (B) = 15.752, p = 0.013], and sweets and ice cream [Exp (B) = 193.633, p = 0.006] were factors inducing overweight or obesity among only African migrant students. CONCLUSION: Controlling the what (Western diet and nature of late-night meals) and the when of eating can drastically reduce their influence on obesogenic condition formation in African migrant students in China and elsewhere.
摘要:
Dietary parboiled rice (PR) has a low risk of disease, but little is known about the contribution of PR to the prevention of hyperlipidemia. The potential role and underlying mechanisms of PR in hyperlipidemia were evaluated in this study. Male C57BL/6J mice were fed with a normal diet, high-fat diet (HFD) containing refined rice (HFDRR) or PR (HFDPR). It was found that PR intervention improved lipid accumulation in mice. Transcriptomic data analysis revealed that 27 genes were up-regulated (mostly involved in lipid breakdown) and 86 genes were down-regulated (mostly involved in inflammatory responses) in the HFDPR group compared to the HFDRR group. And 15 differentially expressed genes (DEGs) were validated by quantitative real-time PCR (RT-qPCR), while protein interaction network showed that protein tyrosine phosphatase receptor type C (PTPRC) has a central role. The gut microbiota of mice was also altered after different dietary treatments, with higher ratio of Firmicutes and Bacteroidetes, increased abundances of Ruminococcaceae, Lachnospiraceae, Christensenellaceae, Porphyromonadaceae, Rikenellaceae and Prevotellaceae, and decreased abundances of Lactobacillaceae, Peptostreptococcaceae, Erysipelotrichaceae and Actinobacteria in the HFDRR group. In addition, it was observed that PPAR signaling pathway may act as a bridge between DEGs and differential gut microbiota. These results suggested that PR can prevent hyperlipidemia by modulating liver genes and gut microbiota. (c) 2024 Beijing Academy of Food Sciences. Publishing services by Tsinghua University Press. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
通讯机构:
[Wu, W ; Wu, XJ] C;Cent South Univ Forestry & Technol, Fac Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词:
Alkaline pH-shifting;Emulsion stability;Epigallocatechin-3-gallate;Rice bran protein;Soybean protein isolate
摘要:
For improving the emulsion stability of rice bran protein (RBP), RBP was modified by different concentrations of epigallocatechin-3-gallate (EGCG) in the presence of soybean protein isolate (SPI), and RBP-EGCG-SPI conjugate was prepared by alkaline pH-shifting. The results showed that the addition of EGCG led to an increase in the bound phenol content and the flexibility of the secondary structure, a decrease in the free sulfhydryl and disulfide bond content of the RBP-EGCG-SPI conjugate. EGCG covalently bound to RBP and SPI through non-disulfide bonds. When the concentration of EGCG was 10% (w/v), the emulsifying activity index and emulsion stability index of conjugate reached the maximum value (36.61m(2)/g and 255.61min, respectively), and the conjugate had the best emulsion stability. However, an EGCG concentration above 10% (w/v) negatively affected the emulsion stability, with increasing particle size due to protein aggregation. Summarily, the modification of EGCG improved the emulsion stability of conjugate by regulating the spatial structure of RBP-EGCG-SPI conjugate. The work provided an important guide to further improve the emulsion stability of RBP.
摘要:
As one of the major reactive oxygen species (ROS), superoxide anion (O-2(center dot-)) is engaged in maintaining redox homeostasis in the cell microenvironment. To identify the pathological roles in related disorders caused by abnormal expression of O-2(center dot-), it is of great significance to monitor and track the fluctuation of O-2(center dot-) concentration in vivo. However, the low concentration of O-2(center dot-) and the interference caused by tissue autofluorescence make the development of an ideal detection methodology full of challenges. Herein, a "Turn-On" chemical response near-infrared (NIR) fluorescence probe Dcm-Cu-OTf for O-2(center dot-) detection in inflamed models, was constructed by conjugating the NIR fluorophore (dicyanisophorone derivative) with an O-2(center dot-) sensing moiety (trifluoromethanesulfonate). Dcm-Cu-OTf exerted about 140-fold fluorescence enhancement after reacting 200 mu M O-2(center dot-) with an excellent limited of detection (LOD) as low as 149 nM. Additionally, Dcm-Cu-OTf exhibited a super large Stokes shift (260 nm) and high selectivity over other bio-analytes in stimulated conditions. Importantly, Dcm-Cu-OTf showed low toxicity and enabled imaging of the generation of O-2(center dot-) in the Lipopolysaccharide (LPS)-stimulated HeLa cells, zebrafish, and LPS-induced inflamed mice. The present study provided a potential and reliable detection tool to inspect the physiological and pathological progress of O-2(center dot-) in living biosystems.
期刊:
Chemical Engineering Journal,2024年479:147753 ISSN:1385-8947
通讯作者:
Guo, X;Wu, YQ
作者机构:
[Zhang, Xuefeng; Guo, Xin; Qi, Xinmiao; Ouyang, Jiayu] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.;[Yi, Xueqian] Hunan Inst Traff Engn, Hengyang 421200, Peoples R China.;[Wu, Yiqiang; Wang, Shiqi; Jiang, Ping] Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Peoples R China.;[Xu, Dong] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Peoples R China.
通讯机构:
[Wu, YQ ; Guo, X ] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Peoples R China.
关键词:
Hydrogel;Adsorption;Detection;Iodide
摘要:
To remove iodine, a novel chitosan/carbon dots-loaded nanocellulose/layered double hydroxide composite hydrogel (CCNL) was successfully synthesized to enable the simultaneous detection and removal of the iodide ion (I-). Adsorption experiments revealed that the CCNL exhibited an impressive maximum adsorption capacity of 231.9 mg/g at 25 degrees C. The adsorption isotherms closely conformed to the Langmuir model, and the adsorption kinetics followed the pseudo-second-order model. The CCNL demonstrated outstanding reusability, maintaining over 89.7 % of its initial adsorption capacity after six cycles. Furthermore, it exhibited remarkable temporal stability, preserving over 91.0 % of its initial adsorption capacity after storage for 15 weeks. In addition, the CCNL exhibited remarkable selectivity and sensitivity in detecting I-, with a low limit of detection (0.09 mu g/L) within the linear ranges of 5-150 mg/L and 0.2-1.0 mu g/L. Fluorescence quenching was attributed to a dynamic process. Importantly, cost analysis revealed that the cost of the removal of I- per gram using the CCNL was only CNY 0.16 (USD 0.02). This was significantly lower than the cost of removal using activated carbon, which was CNY 3.87 (USD 0.53). This study provides a foundation for the development and production of a long-term, stable, reusable, cost-effective, and multifunctional adsorbent for managing iodide pollution.
摘要:
Resveratrol has several functions, including protection of the heart and nervous system and exerts antidiabetic, anti-inflammatory, anti-aging, and antitumor effects. It is reported to impede the occurrence and development of tumors in cancer cell lines, animal models, and clinical studies. In vitro and in vivo experiments show that it exerts preventive or adjuvant therapeutic effects in pancreatic, colorectal, prostate, liver, and lung cancers. Mechanistic research reports show that resveratrol can induce tumor cell apoptosis and autophagy, inhibit cell cycle and angiogenesis, regulate nuclear factors and cyclooxygenase signal transduction pathways, and inhibit carcinogens' metabolic activation and alter tumor-related expression patterns; anti-oxidation affects tumor cell proliferation, metastasis, and apoptosis. However, the exact mechanism underlying its action remains unclear. This review highlights multiple aspects of the biological impacts and mechanisms underlying resveratrol action on the occurrence and development of lung cancer.
摘要:
Although walnut protein (WP) is a good source of plant protein, it has a low degree of industrial development and utilization. In this study, we attempted to develop an edible 3D printing WP emulsion using walnut protein for processing and further preparation of walnut protein-based cream alternative. We investigated the feasibility of 3D printing for producing a visually attractive and nutrient-rich walnut protein-based cream substitute through the addition of xanthan gum (XG) (0.0, 0.5, 1.0, 1.5, 2.0 and 2.5%, w/w). The results showed that the addition of XG in WP emulsion promoted the formation of hydrogen bonds, which significantly improved gel strength, water holding capacity, mechanical strength, stability, printability, significant shear thinning behavior, and high solid viscoelasticity. After testing, the walnut protein emulsion containing 1.5% XG had high printing accuracy, high self-supporting properties, and smooth surface texture. Walnut protein shows a promising cream substitute, which provides a basis for its industrial development.