关键词：
Cadmium ion;Enrichment and removal;Fluorescent probe;Functionalized glass slide
摘要：
Cadmium (Cd) is a widespread and highly toxic environmental pollutant, seriously threatening animal and plant growth. Therefore, monitoring and employing robust tools to enrich and remove Cd from the environment is a major challenge. In this work, by conjugating a fluorescent indicator (CCP) with a functionalized glass slide, a special composite material (CCPB) was constructed to enrich, remove, and monitor Cd2+ in water rapidly. Then Cd2+ could be effectively eluted by immersing the Cd-enriched CCPB in an ethylenediaminetetraacetic acid (EDTA) solution. With this, the CCPB was continuously reused. Its recovery of Cd2+was above and below 100 % after multiple uses by flame atomic absorption spectrometry (FAAS), which was excellent for practical use in enriching and removing Cd2+ in real aqueous samples. Therefore, CCPB is an ideal material for monitoring, enriching, and removing Cd2+ in wastewater, providing a robust tool for future practical applications of Cd enrichment and removal in the environment.
摘要：
The deterioration of food freshness and occurrence of spoilage is attributed to adverse external conditions during storage, leading to unnecessary waste and posing food safety concerns. Therefore, it is imperative to develop packaging that monitors and maintains food freshness. Intelligent and active packaging precisely meets these desired requirements. Intelligent packaging has the potential to offer real-time feedback on environmental changes within the packaging, providing valuable information about food quality and safety. On the other hand, active packaging represents a novel approach that incorporates active ingredients, such as antimicrobials into packages to enhance food quality. This review highlights recent advancements in dual -functional packaging utilizing various plant essential oils and pH -sensitive natural pigments. It explores different methods, with a focus on electrospinning, and also discusses existing challenges and future trends. This information aims to assist in the development of packaging systems, offering a new direction for the development of antibacterial and pHsensitive packaging.
期刊：
International Journal of Biological Macromolecules,2024年:131498 ISSN：0141-8130
通讯作者：
Luo, Feijun;Lin, Qinlu
作者机构：
[Yang, Feiyan; Wu, Yuchi; Qin, Dandan; Hu, Zuomin] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;[Luo, Yidan] College of Life Science and Technology, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;[Luo, Feijun] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China. Electronic address: luofeijun888@csuft.edu.cn;[Lin, Qinlu] College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China. Electronic address: linqinlu@hotmail.com
通讯机构：
[Luo, Feijun; Lin, Qinlu] C;College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China. Electronic address:
摘要：
Momordica charantia L. is a kind of vegetable with medicinal value. As the main component of the vegetable, Momordica charantia polysaccharides (MCPs) mainly consist of galactose, galacturonic acid, xylose, rhamnose, mannose and the molecular weight range is 4.33 × 10(3)-1.16 × 10(6) Da. MCPs have been found to have various biological activities in recent years, such as anti-oxidation, anti-diabetes, anti-brain injury, anti-obesity, immunomodulatory and anti-inflammation. In this review, we systematically summarized the extraction methods, structural characteristics and physicochemical properties of MCPs. Especially MCPs modulate gut microbiota and cause the alterations of metabolic products, which can regulate different signaling pathways and target gene expressions to exert various functions. Meanwhile, the potential structure-activity relationships of MCPs were analyzed to provide a scientific basis for better development or modification of MCPs. Future researches on MCPs should focus on industrial extraction and molecular mechanisms. In East Asia, Momordica charantia L. is used as both food and medicine. It is not clear whether MCP has its unique biological effects. Further study on the difference between MCPs and other food-derived polysaccharides will be helpful to the development and potential application of Momordica charantia L.
摘要：
Octacosanol, a naturally occurring higher fatty alcohol, possessed numerous biological effects. However, octacosanol limited solubility in water due to its lipophilic nature and large structure, resulting in poor absorption and low bioavailability. To overcome this challenge, we developed a simple, environmentally friendly, and energy-efficient O/W nanoemulsion synthesis process. The nanoemulsion achieved an average droplet size of approximately 30nm, exhibited excellent dispersibility and stability at room temperature for 60days, and showcased robust storage properties insensitive to ambient temperature, pH, NaCl, and sucrose. Remarkably, the preparation process of the nanoemulsion maintained the biological activity of octacosanol while demonstrating significantly enhancing antioxidant activity compared to octacosanol suspension. Additionally, the nanoemulsion displayed negligible cytotoxic effects on Caco-2 cells. Significantly, the octacosanol nanoemulsion exhibited a 5.4-fold enhancement in transmembrane transport efficiency when compared to the suspension in Caco-2 cell monolayers. Additionally, in an in vivo experiment, there was a notable 2.9-fold increase in rat intestinal absorption. These findings could provide valuable insights into the development of octacosanol nanoemulsion, supporting its future applications and paving the way for the design of stable nanoemulsion systems for other lipophilic and sparingly soluble substances.
摘要：
Myocardial ischemia-reperfusion (MI/R) not only causes cardiac damage, but also causes severe renal damage. T8 is the 8th peptide identified by peptiomics in digested yak milk dregs and our previous studies showed that T8 had strong antioxidant activity. This study evaluated the protective effects and molecular mechanisms of MI/R-induced kidney injury in rats. Our results indicated that peptide T8 could increase ejection fraction (EF) and shortened fraction (FS), and degraded ST segment elevation, which ameliorated cardiac function in the MI/R rats. Peptide T8 could increase activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX), and decreased the contents of malondialdehyde (MDA), serum creatinine (Scr) and blood urea nitrogen (BUN), which could ameliorate renal insufficiency. Peptide T8 and kidney injury-related targets in the MI/R-damaged rats were obtained from network pharmacology analysis. KEGG analysis revealed that T8 might affect 54 signaling pathways and 13 key targets were obtained by PPI network analysis. The binding affinity of peptide T8 to Keap1 was found to be the strongest by molecular docking analysis. In the H2O2-induced HEK293 cell model, peptide T8 decreased reactive oxygen species (ROS) content and changed the expression ratio of Bcl-2 and Bax, thereby inhibiting mitochondria-dependent apoptosis. Further studies indicated that T8 could regulate Nrf2 pathway and downstream target genes such as NQO1, which could reduce oxidative stress-induced damage. These results suggest that peptide T 8 can exert renal protection via regulating Nrf2 pathway and apoptosis-related genes.
摘要：
In this work, we have developed a composite chitosan film incorporating the Litsea cubeba essential oil (LCEO) and starch with good physical properties, and investigated the effect of coating strawberries with this composite film. The best formula of the LCEO/chitosan/corn starch/glycerol (LCEO/CH/CS/gly) composite films is 0.25% LCEO, 2.75% CH, 0.40% corn starch, and 0.75% glycerol. Coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly films resulted in significantly lower respiration intensity and a slower decay rate, much slower decreases in the firmness, and reductions in the sugar and ascorbic acid content of the fruit during storage (p < 0.05). The coatings also led to a much slower accumulation of malondialdehyde and anthocyanins (p < 0.05). The LCEO/CH/CS/gly film was generally more effective than the CH/CS/gly film; however, the effect was more obvious in the later stages of storage. Thus, coating strawberries with CH/CS/gly film or LCEO/CH/CS/gly film can be a viable method for extending the shelf-life of the fruit.
摘要：
Inflammatory bowel disease (IBD) includes ulcerative colitis and Crohn's disease, and it is a multifactorial disease of the intestinal mucosa. Oxidative stress damage and inflammation are major risk factors for IBD. Vitamin E has powerful antioxidant and anti-inflammatory effects. Our previous work and other investigations have shown that vitamin E has a positive effect on the prevention and treatment of IBD. In this paper, the source and structure of vitamin E and the potential mechanism of vitamin E's role in IBD were summarized, and we also analyzed the status of vitamin E deficiency in patients with IBD and the effect of vitamin E supplementation on IBD. The potential mechanisms by which vitamin E plays a role in the prevention and treatment of IBD include improvement of oxidative damage, enhancement of immunity, maintenance of intestinal barrier integrity, and suppression of inflammatory cytokines, modulating the gut microbiota and other relevant factors. The review will improve our understanding of the complex mechanism by which vitamin E inhibits IBD, and it also provides references for doctors in clinical practice and researchers in this field.
摘要：
A novel method to evaluate rice drying method was investigated based on water migration and molecular motion law analysis. Three typical drying methods were selected, including continuous constant temperature drying at 50 degrees C (CCTD), constant temperature drying at 50 degrees C combined with tempering at 50 degrees C (CTD-T), and constant temperature drying at 50 degrees C combined with tempering at 65 degrees C (CTD-HT). Before sample analysis, single-husked rice was cut into four parts equally with its central axis. During rice drying, the smallest cytoplasmic viscosity (1.28 x 10-10 poise) was observed in CTD-HT samples, compared with CCTD (1.45 x 10-10 poise) and CTD-T (1.36 x 10-10 poise) samples, which resulted in the highest drying efficiency of CTD-HT. After rice drying, the smallest moisture gradients (only 1.25%) between adjacent parts of rice and the lower molecular mobility were found in CTD-HT samples, which gave rise to the highest physicochemical, texture, and storage quality of dried rice. Optimal drying method can be identified quickly and accurately as CTD-HT. Reliability and accuracy of the evaluation method were confirmed by the classic methods.
摘要：
Enzymatic hydrolysis lignin residues (EHLRs) of lignocellulose usually adsorbs cellulase, which can be recycled and used to replace parts of cellulase in the hydrolysis process. To understand this phenomenon during enzymatic hydrolysis (EH) of sugarcane bagasse (SCB) treated with sulfite (SPORL) and dilute acid (DA), the adsorption characteristics between lignin and cellulase in EHLRs were investigated, focusing on interaction force at molecular level and enzymatic activity. The results revealed that SPORL-EHLR adsorbed more beta-glucosidases (beta-GLs) through the stronger electrostatic attraction and hydrogen bond force, causing higher cellulase adsorption amount compared to DA-EHLR. Further exploration demonstrated that the cellobiose's catalytic and binding sites on beta-GLs were separated from the binding site of SPORL-EHLR on beta-GLs, resulting in minimal in-hibition of beta-GLs activity when bound to SPORL-EHLR. Furthermore, adding SPORL-EHLR in SCB hydrolysis saved 40% cellulase. This study deepens the understanding of the adsorption behavior between lignin and cellulase.
摘要：
This work innovatively used cellulose nanofibers as a photocatalyst carrier, which could recycle nano-photocatalysts and minimize nanoparticle aggregation. The morphology, structures, chemical composition, optical-electronic properties and photocatalytic performance of amino-modified carbon quantum dots-ZnO/cellulose nanofiber (N-CQDs-ZnO/CNF: ZCH-2) hydrogel were characterized by SEM, TEM, BET, EDS, XRD, FTIR, UV-vis, XPS, PL and other techniques. The mechanism of Cr(VI) adsorption synergistic photoreduction by ZCH-2 was discussed in detail. The results showed that the prepared ZCH-2 had excellent removal performance for Cr(VI). After 120min of adsorption and 40min of photoreduction, the removal efficiency of Cr(VI) was 98.9%. Compared with ZnO/CNF hydrogel, the adsorption performance of ZCH-2 increased by 268% and the photoreduction performance increased by 116%. The adsorption of Cr(VI) by ZCH-2 was controlled by electrostatic attraction and chemical adsorption. The photoreduction kinetic constant of ZCH-2 was 0.106min(-1), which was 8.9 times that of ZnO/CNF hydrogel. The N-CQDs in ZCH-2 could form N-CQDs-metal complexes with Cr(VI), resulting in fluorescence quenching, so Cr(VI) could be visually identified by fluorescence changes. This study provides a new idea for the design and optimization of a new multifunctional hydrogel with efficient adsorption-photoreduction-fluorescence recognition.
摘要：
With the increase in people's living standards, the number of patients suffering from liver injury keeps on increasing. Traditional diagnostic methods can no longer meet the needs of early and accurate diagnosis due to their limitations in application. However, fluorescent probes based on different fluorophores and nanomaterials have been gradually lighting up medical research due to their unique properties, such as high specificity and non-invasiveness. In addition, accurate identification of the different types of liver injury biomarkers can significantly improve the level of early diagnosis. Therefore, this review reviews the fluorescent probes used in the detection of biomarkers of liver injury over recent years and briefly summarizes the corresponding biomarkers of different types of liver injury. Impressively, this review also lists the structures and the response mechanisms of the different probes, and concludes with an outlook, suggesting directions in which improvements can be made. Finally, we hope that this review will contribute to the further development of fluorescent probes for the early diagnosis and assessment of liver injury. With the increase in people's living standards, the number of patients suffering from liver injury keeps on increasing.
通讯机构：
[Li, P ] N;Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Collaborat Innovat Ctr Modern Grain Circulat & Saf, Nanjing, Peoples R China.
关键词：
Apple juice;Hg(II) removal;Nano‑selenium functionalized chitosan gel beads
摘要：
The presence of potentially toxic elements and compounds poses threats to the quality and safety of fruit juices. Among these, Hg(II) is considered as one of the most poisonous heavy metals to human health. Traditional chitosan-based and selenide-based adsorbents face challenges such as poor adsorption capacity and inconvenient separation in juice applications. In this study, we prepared nano‑selenium functionalized chitosan gel beads (nanoSe@CBs) and illustrated the synergistic promotions between chitosan and nanoSe in removing Hg(II) from apple juice. The preparation conditions, adsorption behaviors, and adsorption mechanism of nanoSe@CBs were systematically investigated. The results revealed that the adsorption process was primarily controlled by chemical adsorption. At the 0.1% dosage, the adsorbent exhibited high uptake, and the maximum adsorption capacity from the Langmuir isotherm model could reach 376.5mg/g at room temperature. The adsorbent maintained high adsorption efficiency (> 90%) across a wide range of Hg(II) concentrations (0.01 to 10mg/L) and was unaffected by organic acids present in apple juice. Additionally, nanoSe@CBs showed negligible effects on the quality of apple juice. Overall, nanoSe@CBs open up possibilities to be used as a safe, low-cost and highly-efficient adsorbent for the removal of Hg(II) from juices and other liquid foods.
摘要：
Screening and identifying the active compounds in foods are important for the development and utilization of functional foods. In this study, the anti-enteritis activity of ethanol extract from Camellia oleifera oil (PECS) was quickly evaluated using a Smurf Drosophila model and the metabolomics approach, combined with molecular docking techniques, were performed to rapidly screen and identify compounds with potential anti-enteritis activity in PECS. PECS showed good anti-enteritis activity and inhibited the activity of 5-lipoxygenase (LOX), cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS). In particular, wighteone and p-octopamine were newly identified in C. oleifera oil and were proven to have good anti-enteritis activity. The inhibitory activity of kaempferitrin (IC(50) = 0.365 mmol L(-1)) was higher than that of wighteone (IC(50) = 0.424 mmol L(-1)) and p-octopamine (IC(50) = 0.402 mmol L(-1)). Of note, the IC(50) value of salazosulfapyridine was 0.810 mmol L(-1). Inhibition of LOX activity is likely one of the anti-enteritis mechanisms of PECS. These new findings lay the foundation for further investigations into the underlying mechanisms of anti-enteritis activity in C. oleifera oil.
摘要：
Unconventional feed, which is abundant in China, contains anti-nutritional factors and toxins; however, these can be greatly reduced with microbial fermentation, thus improving the nutrient content of the feed, enhancing animal appetites, and ultimately significantly improving the intestinal health and growth performance of animals. When oxidative stress occurs, fermented feed can effectively reduce the damage caused by stress to the gastrointestinal tract, accelerate the removal of gastrointestinal abnormalities, improve the ability to resist intestinal stress, and ensure the efficient production of animals. This review introduces the application of unconventional fermented feed in animal production, and expounds upon the function of unconventional fermented feed in animals with oxidative stress symptoms, so as to provide a theoretical reference for the development and application of unconventional fermented feed in antioxidative stress reduction.
关键词：
Osteoblasts proliferation and differentiation;Polysaccharide;Sparassis crispa;Structural characterization
摘要：
This study aimed to investigate the structural characterization of water-soluble polysaccharides from Sparassis crispa and their effects on the proliferation and differentiation of mouse osteoblasts. Three fractions (F-1, F-2, and F-3) were obtained from crude polysaccharides by a DEAE-52 cellulose column. The main fraction (F-1) was further purified by polysaccharide gel purification systems to obtain purified water-soluble Sparassis crispa polysaccharide (SCPS). The chemical structure of SCPS was analyzed by gas chromatography, Fourier transform infrared spectroscopy, methylation analysis, and nuclear magnetic resonance spectroscopy. The monosaccharide compositional analysis revealed that SCPS consisted of fucose, arabinose, galactose, glucose, xylose, mannose, ribose, galacturonic acid, glucuronic acid, and mannuronic acid in a molar ratio of 17.37:1.94:25.52:30.83:1.14:0.30:4.98:2.87:2.65. Moreover, the backbone of SCPS was composed of →3)-β-d-Glcp-(1→4)-β-d-Glcp-(1→, with side chains attached to the backbone at the O-6 positions through the →3,6)-β-d-Glcp-(1→ linkage. The in vitro experiments were conducted to investigate the effects of SCPS on the proliferation and differentiation of mouse osteoblasts. The results showed that SCPS significantly enhanced the proliferation and differentiation of mouse osteoblasts, indicating their potential as a pharmaceutical agent for promoting osteoblast proliferation and differentiation.
