期刊论文

Study on Diversity and the Activity of Producing Digestive Enzymes of Culturable Intestinal Bacteria from Sitophilus Oryzae

中文

青海农林科技

1004-9967

2023

01

1-5+12

10.3969/j.issn.1004-9967.2023.01.001

19B597:湖南省教育厅科学研究项目 CX20200720:湖南省研究生科研创新项目

本校为第一机构

为明确米象肠道可培养细菌的多样性及产酶功能,本研究采用传统分离培养的方法分别对米象成虫和末龄幼虫肠道细菌进行了分离培养,并结合16S rDNA序列进行系统发育分析,同时采用透明圈法测定了肠道可培养细菌的产酶活性。结果显示...展开更多 为明确米象肠道可培养细菌的多样性及产酶功能,本研究采用传统分离培养的方法分别对米象成虫和末龄幼虫肠道细菌进行了分离培养,并结合16S rDNA序列进行系统发育分析,同时采用透明圈法测定了肠道可培养细菌的产酶活性。结果显示,米象成虫和末龄幼虫肠道共分离出细菌23株,分属于1门(厚壁菌门Firmicutes)2属(芽孢杆菌属Bacillus、葡萄球菌属Staphylococcus),其...

In order to clarify the diversity and enzyme-producing function of culturable intestinal bacteria of Sitophilus oryzae,the traditional method was used to isolate and culture the bacteria in this study.Diversity analysis was performed in combination with 16S rDNA sequenc...MORE In order to clarify the diversity and enzyme-producing function of culturable intestinal bacteria of Sitophilus oryzae,the traditional method was used to isolate and culture the bacteria in this study.Diversity analysis was performed in combination with 16S rDNA sequences for phylogenetic analysis.The transparent circle ...