橘皮黄酮提取工艺优化及其对羟基自由基诱导的SH-SY5Y细胞凋亡的抑制作用研究
作者：
张雅丹;赵梦倩;张琳;周彬彬
期刊：
粮食与油脂 ,2021年34(01):123-127 ISSN：1008-9578
作者机构：
[张雅丹; 赵梦倩; 张琳] 中南林业科技大学食品科学与工程学院特医食品加工湖南省重点实验室;[周彬彬] 湖南省食品质量监督检验研究院
关键词：
橘皮黄酮;提取;羟基自由基;神经母细胞瘤细胞(SH-SY5Y)
摘要：
以酶解超声提取法提取橘皮黄酮,通过单因素和正交试验研究纤维素酶质量浓度、超声时间、乙醇体积浓度、料液比对黄酮得率的影响,确定橘皮黄酮的最佳提取条件:纤维素酶质量浓度0.5 g/mL、超声时间60 min、乙醇体积浓度90%、料液比1∶60（g/mL）。以香豆素-3-羧酸（Coumarin-3-carboxylic acid,CCA）荧光法和噻唑蓝（Methyl thiazoly1 tetrazolium,MTT）试验发现橘皮黄酮在浓度为20～100μg/mL范围内能有效清除Cu2+催化H2O2及抗坏血酸产生的羟基自由基并有效抑制SH-SY5Y细胞的凋亡,且其作用效果呈浓度依赖性。
语种：
中文
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A label-free fluorescent peptide probe for sensitive and selective determination of copper and sulfide ions in aqueous systems
作者：
Zhang, Yadan;Cai, Yunhui;He, Yonghui;Lin, Qinlu;Ren, Jiali;...
期刊：
RSC Advances ,2021年11(13):7426-7435 ISSN：2046-2069
通讯作者：
Zhang, Lin
作者机构：
[Cai, Yunhui; Ren, Jiali; Zhang, Yadan; Zhang, Lin; Lin, Qinlu] Cent South Univ Forestry & Technol, Sch Food Sci & Engn, Hunan Key Lab Forestry Edible Resources Safety &, Natl Engn Lab Rice & Byprod Deep Proc,Hunan Key L, Changsha 410004, Peoples R China.;[He, Yonghui] Yunnan Minzu Univ, Key Lab Chem Ethn Med Resources, State Ethn Affairs Commiss, Kunming 650500, Yunnan, Peoples R China.;[He, Yonghui] Yunnan Minzu Univ, Minist Educ, Kunming 650500, Yunnan, Peoples R China.;[Cao, Dongsheng] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha 410083, Peoples R China.
通讯机构：
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Sch Food Sci & Engn, Hunan Key Lab Forestry Edible Resources Safety &, Natl Engn Lab Rice & Byprod Deep Proc,Hunan Key L, Changsha 410004, Peoples R China.
摘要：
A label free fluorescent peptide probe (HDSGWEVHH) was used for Cu2+and S2−determination in aqueous solution. Our results demonstrated that HDSGWEVHH is highly selective and sensitive for monitoring free Cu2+concentrationviaquenching of the probe fluorescence upon Cu2+binding. The mechanism of the complexation is investigated with Cyclic Voltammetry (CV),1H nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR) spectroscopy and computational techniques. Theoretical calculation results indicated the binding ratio of the probe to Cu2+is 2 : 1 and the binding constant was obtained as 1.72 × 108M−1. Cu2+concentration can be detected with the detection limit of 16 nM. Free Cu2+concentration released from the metallothionein-Cu complex at different pH values was detected. Cu2+concentration in real water and tea samples was also detected, and the results were consistent with the ones monitored by atomic absorption spectrometer. Because of the exceedingly smallKspvalue of CuS (1.27 × 10−36), S2−can sequester Cu2+from HDSGWEVHH to restore the tryptophan (W) fluorescence. Thus the HDSGWEVHH-Cu2+complex can also be used for S2−detection. The S2−concentrations can be monitored with a detection limit of 19 nM. The assay is also amenable to measurement of S2−concentration in pure water samples. Thus the probe designed herein is sensitive, label free, low cost, and environmentally friendly for Cu2+and S2−determination in aqueous solutions. © The Royal Society of Chemistry 2021.
语种：
英文
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Preparation and characterization of broken-rice starch nanoparticles with different sizes
作者：
Xiao, Huaxi;Yang, Fan;Lin, Qinlu* ;Zhang, Qian;Zhang, Lin;...
期刊：
International Journal of Biological Macromolecules ,2020年160:437-445 ISSN：0141-8130
通讯作者：
Lin, Qinlu;Liu, Gao-Qiang
作者机构：
[Han, Wenfang; Xiao, Huaxi; Zhang, Qian; Liu, Gao-Qiang; Zhang, Lin; Sun, Shuguo; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Peoples R China.;[Yang, Fan] Shanxi Technol & Business Coll, Taiyuan 030006, Peoples R China.;[Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Peoples R China.;[Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Peoples R China.;[Lin, Qinlu; Liu, GQ] 498 Southern Shaoshan Rd, Changsha 410004, Peoples R China.
通讯机构：
[Lin, QL; Liu, GQ] 4;498 Southern Shaoshan Rd, Changsha 410004, Peoples R China.
关键词：
Broken rice;Starch nanoparticles;Particle diameter;Water absorption;Crystalline structure
摘要：
Broken-rice starch nanoparticles with different mean particle diameters for 100, 200, 400 and 800 nm were prepared by nanopredpitation, alkali freezing, cross-linking and H2SO4 hydrolysis methods respectively, and their structural, morphological and physicochemical properties were systematically characterized. The results showed that broken-rice starch nanoparticles had higher water absorption rate, and the maximum water absorption rate was obtained from the 100 nm starch granules being 91.53%, which means an increase about 2.07-fold in water absorption rate as compared with native rice starch. The stability of native rice starch is the worst, but the viscosity characteristic value is always higher than that of starch nanoparticles in the whole gelatinization process. The FT-IR spectrum showed that only starch nanoparticles prepared by cross-linking method showed the characteristic peak of secondary amide structure at 1714 cm(-1), but the structure of was basically the same as native starch. The X-ray diffraction pattern revealed that there were obvious characteristic diffraction peaks near 20 for 15 degrees, 1 7 degrees 19 degrees and 23 degrees for the 800 nm starch nanoparticles and native rice starch, while the characteristic diffraction peaks of other starch nanoparticles disappeared in varying degrees due to the changed crystal structure. (C) 2020 Elsevier B.V. All rights reserved.
语种：
英文
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铁皮石斛多糖提取及对羟自由基诱导的SH-SY5Y细胞凋亡的抑制作用
作者：
张雅丹;赵梦倩;杨煜佼;徐友志;王梓郡;...
期刊：
食品科学 ,2020年41(14):286-293 ISSN：1002-6630
作者机构：
特医食品加工湖南省重点实验室,稻谷及副产物深加工国家工程实验室,中南林业科技大学食品科学与工程学院,湖南 长沙 410004;湖南省食品质量监督检验研究院,湖南 长沙 410117;[王梓郡; 杨煜佼; 许迪雅; 王焱君; 周彬彬; 赵梦倩; 徐友志; 张琳; 张雅丹] 中南林业科技大学
关键词：
铁皮石斛多糖;提取;羟自由基;SH-SY5Y细胞
摘要：
以酶解超声提取法提取铁皮石斛多糖,通过对料液比、超声时间、复合酶质量分数的单因素试验和正交试验,确定铁皮石斛多糖的最佳提取条件;以高效液相色谱法对铁皮石斛多糖进行组成成分分析;以香豆素-3-羧酸荧光法测定铁皮石斛多糖的羟自由基清除能力;以噻唑蓝实验和细胞流式实验研究铁皮石斛多糖提取物对SH-SY5Y细胞的保护作用。结果表明,最优铁皮石斛多糖提取条件为料液比1∶100(g/mL)、超声时间2 h、复合酶(纤维素酶和果胶酶1∶1等质量混合)质量分数50%,最优提取条件下铁皮石斛多糖得率为25.69%,其主要单糖组分为甘露糖和葡萄糖。在质量浓度为20~80 μg/mL范围内铁皮石斛多糖提取物能有效清除Cu~(2+)催化H_2O_2产生的羟自由基并有效抑制SH-SY5Y细胞的凋亡(凋亡率从56.97%下降到17.97%),且其作用效果成质量浓度依赖性。因此,铁皮石斛多糖可通过清除羟自由基从而抑制Cu~(2+)催化H_2O_2产生羟自由基诱导的SH-SY5Y细胞凋亡。
语种：
中文
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Fmoc-FF多肽杂化水凝胶对乳糖酶的控释作用研究
作者：
肖冉;赵梦倩;张琳;周彬彬
期刊：
粮食与油脂 ,2020年33(3):86-90 ISSN：1008-9578
作者机构：
特医食品加工湖南省重点实验室;[周彬彬] 湖南省食品质量监督检验研究院;[赵梦倩; 张琳; 肖冉] 中南林业科技大学
关键词：
卡拉胶;杂化水凝胶;乳糖酶;控制释放
摘要：
探讨Fmoc-FF （Phe）多肽与卡拉胶形成的凝胶对乳糖酶在模拟胃液中的保护作用以及对包埋物的控制释放作用。结果表明:在Fmoc-FF与卡拉胶的质量比为1∶15、卡拉胶的质量浓度为48%时,经模拟胃肠道后,杂化水凝胶包埋的乳糖酶的酶活可以达到未经过胃肠道处理的酶活的67.63%,远高于同样实验条件下未经包埋的乳糖酶的酶活（0%）。因此,Fmoc-FF/卡拉胶杂化水凝胶能有效保护乳糖酶不被胃液破坏,且对包埋物有控制释放的作用,可作为口服乳糖酶的控释包埋材料。
语种：
中文
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多肽水凝胶对牛至精油的控制释放及抑菌效果研究
作者：
赵梦倩;张雅丹;张琳;张继红
期刊：
粮食与油脂 ,2020年33(12):76-79 ISSN：1008-9578
作者机构：
特医食品加工湖南省重点实验室;[张继红] 湖南省食品质量监督检验研究院;[赵梦倩; 张琳; 张雅丹] 中南林业科技大学
关键词：
牛至精油;水凝胶;控释
摘要：
研究Fmoc-F多肽水凝胶对牛至精油的包埋作用、控释特性和抑菌效果.结果表明,Fmoc-F水凝胶对牛至精油的包埋率为98.4％,优于β-环糊精(81％).Fmoc-F水凝胶在溶液中的分解率随pH的降低而增大,当pH为5.0时分解率达到100％.溶液中大肠杆菌数量的变化可引起溶液pH的变化,Fmoc-F水凝胶可实现对包埋的牛至精油的控制释放,有效抑制牛至精油的挥发,并提高其抑菌效果.
语种：
中文
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多次湿热处理对大米淀粉结构和性质的影响
作者：
唐玮泽;肖华西;唐倩;林亲录;韩文芳;...
期刊：
中国粮油学报 ,2020年35(10):77-83 ISSN：1003-0174
作者机构：
[唐玮泽; 肖华西; 唐倩; 林亲录; 韩文芳; 张琳; 孙术国; 欧阳倩蓝] Food Science and Engineering of Central South University of Forestry and Technology, Changsha, 410004, China
关键词：
大米淀粉;多次湿热处理;理化性质;微观结构
摘要：
采用多次湿热处理方法对大米淀粉进行物理改性,湿热处理温度110℃,含水量20%,处理时间2 h,对比了不同次数的湿热处理对大米淀粉理化性质的影响。结果表明,随着湿热处理次数的增加,大米淀粉的含水量、膨胀力和溶解度均有所下降且逐渐降低。从糊化性质来看,大米淀粉更难于糊化,结合水的能力下降,热稳定性提高。红外光谱检测结果显示,原大米淀粉和多次湿热处理后的大米淀粉的红外光谱基本没有变化。X-衍射分析结果表明,样品处理前后的结晶型保持不变,但相对结晶度随处理次数增加而逐渐升高。扫描电镜图像显示,经多次湿热处理后,会使淀粉颗粒不再呈现均匀分散的状态,单个颗粒的特征逐渐消失,从而形成新的团状结构。
语种：
中文
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Preparation and properties of hydrophobic films based on acetylated broken-rice starch nanocrystals for slow protein delivery
作者：
Xiao, Huaxi;Yang, Fan;Lin, Qinlu* ;Zhang, Qian;Tang, Weize;...
期刊：
International Journal of Biological Macromolecules ,2019年138:556-564 ISSN：0141-8130
通讯作者：
Lin, Qinlu;Liu, Gao-Qiang
作者机构：
[Xiao, Huaxi; Liu, Gao-Qiang; Zhang, Lin; Xu, Dong; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;[Xiao, Huaxi; Yang, Fan; Zhang, Qian; Tang, Weize; Zhang, Lin; Xu, Dong; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Forestry Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Liu, Gao-Qiang] Cent South Univ Forestry & Technol, Int Cooperat Base Sci & Technol Innovat Forest Re, Changsha 410004, Hunan, Peoples R China.;[Lin, Qinlu; Liu, GQ] 498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Lin, QL; Liu, GQ] 4;498 Southern Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.
关键词：
Acetylation;Broken-rice starch nanocrystals;Drug release
摘要：
Native and acetylated broken-rice starches (nanocrystals) with different degrees of substitution (DS) and their corresponding films were individually prepared, and the drug release profiles, weight loss, solubility and dispersion and surface morphology were comparatively studied. Bovine serum albumin (BSA) was used as a model drug. Acetylated native starch (ANS) DS 2.58, acetylated starch nanocrystals (ASN) DS 0.98, ASN DS 1.86, and ASN DS 2.72 were observed to be very soluble in chloroform. BSA was released rapidly from the native rice starch (NS) and ANS DS 2.58 films. ASN with high DS significantly slowed down the release of BSA from films, the percentages of BSA released from film ASN DS 2.72 only reached to 13% after 3.5?weeks release, and the release data followed Korsmeyer-Peppas equation. Further studies reveal that the particle size of ASN DS 2.72 was smallest, and the weight loss of ASN DS 2.72 film was lowest. The results demonstrate that acetylation and nanometer particle form of rice starch film can effectively retard protein drug release, and the prepared films based on ASN with high DS from broken rice may be suitable for the controlled protein delivery. (C) 2019 Elsevier B.V. All rights reserved.
语种：
英文
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Multi-scale structure and pasting/digestion features of yam bean tuber starches
作者：
Qiao, Dongling;Tu, Wenyao;Liao, Anping;Li, Nannan;Zhang, Binjia* ;...
期刊：
Carbohydrate Polymers ,2019年213:199-207 ISSN：0144-8617
通讯作者：
Zhang, Binjia
作者机构：
[Qiao, Dongling; Jiang, Fatang; Tu, Wenyao] Hubei Univ Technol, Sch Food & Biol Engn, Glyn O Phillips Hydrocolloid Res Ctr HBUT, Wuhan 430068, Hubei, Peoples R China.;[Liao, Anping; Zhong, Lei] Guangxi Univ Nationalities, Dept Chem Engn, Guangxi Key Lab Cultivat Base Polysaccharide Mat, Nanning 530008, Peoples R China.;[Zhang, Binjia; Li, Nannan; Zhao, Siming] Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Grp Cereals & Oils Proc,Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;[Zhang, Liang] Yangzhou Univ, Sch Food Sci & Engn, Yangzhou 225127, Jiangsu, Peoples R China.;[Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhang, Binjia] H;[Zhang, Binjia] J;Huazhong Agr Univ, Minist Educ, Key Lab Environm Correlat Dietol, Grp Cereals & Oils Proc,Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China.;Jinjian Cereals Ind Co Ltd, Changde 415000, Peoples R China.
关键词：
Multi-scale;Physicochemical features;Starch (PubChem CID: 24836924);Structure;Water (PubChem CID: 962);Yam bean tuber starch
摘要：
This work discloses the multi-scale structure and pasting/digestion behaviors of yam bean tuber starches (YB-GD and YB-SC). Compared to potato starch, YB starches exhibited varied pasting/digestion features that were understood from a multi-scale structural view. Especially, YB starches showed relatively polydisperse lamellae, less perfect crystallites, and fewer amylose molecules. These features should reduce the bulk density of starch chain packing, and could facilitate the water or enzyme diffusion in starch matrixes. Consistently, not only was starch's resistance to hydrothermal effects weakened (shown by reduced pasting temperature), but also the enzyme absorption to starch chains and the subsequent hydrolysis events were accelerated. Furthermore, YB starch molecules had more short chains, which played roles in reducing the paste viscosity along with the reduced granule size and in enhancing the paste stabilities during heating and cooling. Also, those molecular features tended to speed up the enzyme diffusion and digestion events.
语种：
英文
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ADMETLAB: A PLATFORM FOR SYSTEMATIC ADMET EVALUATION BASED ON A COMPREHENSIVELY COLLECTED ADMET DATABASE
作者：
Dong, Jie;Wang, Ning-Ning;Yao, Zhi-Jiang;Zhang, Lin;Cheng, Yan;...
期刊：
Journal of Cheminformatics ,2018年10(1):1-11 ISSN：1758-2946
通讯作者：
Cao, Dong-Sheng
作者机构：
[Cheng, Yan; Dong, Jie; Wang, Ning-Ning; Yao, Zhi-Jiang; Cao, Dong-Sheng] Cent South Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;[Dong, Jie] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Natl Engn Lab Deep Proc Rice & Byprod, Changsha, Hunan, Peoples R China.;[Dong, Jie; Zhang, Lin] Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Changsha, Hunan, Peoples R China.;[Cao, Dong-Sheng; Lu, Ai-Ping] Hong Kong Baptist Univ, Sch Chinese Med, Inst Advancing Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China.;[Ouyang, Defang] Univ Macau, ICMS, State Key Lab Qual Res Chinese Med, Macau, Peoples R China.
通讯机构：
[Cao, Dong-Sheng] C;[Cao, Dong-Sheng] H;Cent South Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;Hong Kong Baptist Univ, Sch Chinese Med, Inst Advancing Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China.
关键词：
ADMETlab;ADMET;Drug-likeness;ADMET database;Drug discovery;Cheminformatics
摘要：
Current pharmaceutical research and development (R&D) is a high-risk investment which is usually faced with some unexpected even disastrous failures in different stages of drug discovery. One main reason for R&D failures is the efficacy and safety deficiencies which are related largely to absorption, distribution, metabolism and excretion (ADME) properties and various toxicities (T). Therefore, rapid ADMET evaluation is urgently needed to minimize failures in the drug discovery process. Here, we developed a web-based platform called ADMETlab for systematic ADMET evalua- tion of chemicals based on a comprehensively collected ADMET database consisting of 288,967 entries. Four function modules in the platform enable users to conveniently perform six types of drug-likeness analysis (five rules and one prediction model), 31 ADMET endpoints prediction (basic property: 3, absorption: 6, distribution: 3, metabolism: 10, elimination: 2, toxicity: 7), systematic evaluation and database/similarity searching. We believe that this web platform will hopefully facilitate the drug discovery process by enabling early drug-likeness evaluation, rapid ADMET virtual screening or filtering and prioritization of chemical structures. The ADMETlab web platform is designed based on the Django framework in Python, and is freely accessible at http://admet.scbdd.com/. © The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License.
语种：
英文
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研讨式教学在食品科学类专业课程中的应用研究
作者：
张琳;肖华西;丁玉琴;林亲录
期刊：
教育教学论坛 ,2018年(6):155-156 ISSN：1674-9324
作者机构：
中南林业科技大学食品科学与工程学院稻米及副产物深加工国家工程实验室
关键词：
研讨式教学;食品科学类专业课程;食品法律管理类课程;食品工程类课程;食品分析检测类课程
摘要：
本论文概括介绍了研讨式教学在食品科学类专业课程中的重要性,总结了在不同类别的食品科学类课程中研讨式教学的方法,为研讨式教学在不同食品科学类课程中的应用提供了方向。
语种：
中文
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HAMdb: a database of human autophagy modulators with specific pathway and disease information
作者：
Wang, Ning-Ning;Dong, Jie;Zhang, Lin;Ouyang, Defang;Cheng, Yan;...
期刊：
Journal of Cheminformatics ,2018年10(1):1-8 ISSN：1758-2946
通讯作者：
Cao, Dong-Sheng
作者机构：
[Cheng, Yan; Dong, Jie; Wang, Ning-Ning; Cao, Dong-Sheng] Cent S Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;[Dong, Jie; Zhang, Lin] Cent South Univ Forestry & Technol, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Hunan Key Lab Proc Food Special Med Purpose, Natl Engn Lab Deep Proc Rice & Byprod, Changsha, Hunan, Peoples R China.;[Ouyang, Defang] Univ Macau, State Key Lab Qual Res Chinese Med, Inst Chinese Med Sci, Macau, Peoples R China.;[Chen, Alex F.; Cao, Dong-Sheng] Cent S Univ, Ctr Vasc Dis & Translat Med, Xiangya Hosp 3, Changsha, Hunan, Peoples R China.;[Cao, Dong-Sheng; Lu, Ai-Ping] Hong Kong Baptist Univ, Inst Adv Translat Med Bone & Joint Dis, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China.
通讯机构：
[Cao, Dong-Sheng] C;[Cao, Dong-Sheng] H;Cent S Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;Cent S Univ, Ctr Vasc Dis & Translat Med, Xiangya Hosp 3, Changsha, Hunan, Peoples R China.;Hong Kong Baptist Univ, Inst Adv Translat Med Bone & Joint Dis, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China.
关键词：
Autophagy;Autophagy modulator;Database;Disease;Pathophysiological;Pathway
摘要：
Autophagy is an important homeostatic cellular recycling mechanism responsible for degrading unnecessary or dysfunctional cellular organelles and proteins in all living cells. In addition to its vital homeostatic role, this degradation pathway also involves in various human disorders, including metabolic conditions, neurodegenerative diseases, cancers and infectious diseases. Therefore, the comprehensive understanding of autophagy process, autophagy-related modulators and corresponding pathway and disease information will be of great help for identifying the new autophagy modulators, potential drug candidates, new diagnostic and therapeutic targets. In recent years, some autophagy databases providing structural and functional information were developed, but the specific databases covering autophagy modulator (proteins, chemicals and microRNAs)-related target, pathway and disease information do not exist. Hence, we developed an online resource, Human Autophagy Modulator Database (HAMdb, http://hamdb.scbdd.com), to provide researchers related pathway and disease information as many as possible. HAMdb contains 796 proteins, 841 chemicals and 132 microRNAs. Their specific effects on autophagy, physicochemical information, biological information and disease information were manually collected and compiled. Additionally, lots of external links were available for more information covering extensive biomedical knowledge. HAMdb provides a user-friendly interface to query, search, browse autophagy modulators and their comprehensive related information. HAMdb will help researchers understand the whole autophagy process and provide detailed information about related diseases. Furthermore, it can give hints for the identification of new diagnostic and therapeutic targets and the discovery of new autophagy modulators. In a word, we hope that HAMdb has the potential to promote the autophagy research in pharmacological and pathophysiological area. © 2018, The Author(s).
语种：
英文
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在《食品工程原理》课程教学中培养学生的工程意识
作者：
丁玉琴;胡佳;张琳;周文化;林亲录
期刊：
课程教育研究 ,2018年(39):245-246 ISSN：2095-3089
作者机构：
中南林业科技大学信息中心;中南林业科技大学食品科学与工程学院;[胡佳; 周文化; 林亲录; 张琳; 丁玉琴] 中南林业科技大学
关键词：
工程意识;《食品工程原理》;教学改革
摘要：
食品工程原理是一门理论性和实践性并重的专业基础课,如何能让学生学好这门课的同时提高工程意识是教学改革中需要思考的重要问题。本文以学生工程意识培养为目标对食品工程原理的教学提出了几点问题及改革措施。
语种：
中文
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PyBioMed: a python library for various molecular representations of chemicals, proteins and DNAs and their interactions
作者：
Dong, Jie;Yao, Zhi-Jiang;Zhang, Lin;Luo, Feijun;Lin, Qinlu;...
期刊：
Journal of Cheminformatics ,2018年10(1):1-11 ISSN：1758-2946
通讯作者：
Cao, Dong-Sheng
作者机构：
[Dong, Jie; Yao, Zhi-Jiang; Cao, Dong-Sheng] Cent S Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;[Dong, Jie; Luo, Feijun; Zhang, Lin; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Deep Proc Rice & Byprod, Changsha, Hunan, Peoples R China.;[Cao, Dong-Sheng; Lu, Ai-Ping] Hong Kong Baptist Univ, Sch Chinese Med, Inst Adv Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China.;[Chen, Alex F.; Cao, Dong-Sheng] Cent S Univ, Xiangya Hosp 3, Ctr Vasc Dis & Translat Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Cao, Dong-Sheng] C;[Cao, Dong-Sheng] H;Cent S Univ, Xiangya Sch Pharmaceut Sci, 172 Tongzipo Rd, Changsha, Hunan, Peoples R China.;Hong Kong Baptist Univ, Sch Chinese Med, Inst Adv Translat Med Bone & Joint Dis, Hong Kong, Hong Kong, Peoples R China.;Cent S Univ, Xiangya Hosp 3, Ctr Vasc Dis & Translat Med, Changsha, Hunan, Peoples R China.
关键词：
Molecular representation;Molecular descriptors;Python library;Chemoinformatics;Data integration;Bioinformatics
摘要：
Background: With the increasing development of biotechnology and informatics technology, publicly available data in chemistry and biology are undergoing explosive growth. Such wealthy information in these data needs to be extracted and transformed to useful knowledge by various data mining methods. Considering the amazing rate at which data are accumulated in chemistry and biology fields, new tools that process and interpret large and complex interaction data are increasingly important. So far, there are no suitable toolkits that can effectively link the chemical and biological space in view of molecular representation. To further explore these complex data, an integrated toolkit for various molecular representation is urgently needed which could be easily integrated with data mining algorithms to start a full data analysis pipeline. Results: Herein, the python library PyBioMed is presented, which comprises functionalities for online download for various molecular objects by providing different IDs, the pretreatment of molecular structures, the computation of various molecular descriptors for chemicals, proteins, DNAs and their interactions. PyBioMed is a feature-rich and highly customized python library used for the characterization of various complex chemical and biological molecules and interaction samples. The current version of PyBioMed could calculate 775 chemical descriptors and 19 kinds of chemical fingerprints, 9920 protein descriptors based on protein sequences, more than 6000 DNA descriptors from nucleotide sequences, and interaction descriptors from pairwise samples using three different combining strategies. Several examples and five real-life applications were provided to clearly guide the users how to use PyBioMed as an integral part of data analysis projects. By using PyBioMed, users are able to start a full pipelining from getting molecular data, pretreating molecules, molecular representation to constructing machine learning models conveniently. Conclusion: PyBioMed provides various user-friendly and highly customized APIs to calculate various features of biological molecules and complex interaction samples conveniently, which aims at building integrated analysis pipelines from data acquisition, data checking, and descriptor calculation to modeling. PyBioMed is freely available at http://projects.scbdd.com/pybiomed.html. © 2018, The Author(s).
语种：
英文
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鱼露特征香气及增香微生物研究进展
作者：
贺海翔;徐莉娜;付湘晋;吴跃;张琳;...
期刊：
食品安全质量检测学报 ,2018年9(8):1776-1781 ISSN：2095-0381
作者机构：
[贺海翔] 湖南宏兴隆湘莲食品有限公司;中南林业科技大学食品科学与工程学院;[吴跃; 徐莉娜; 付湘晋; 丁玉琴; 张琳] 中南林业科技大学
关键词：
鱼露;香气活性物质;微生物;氨基酸代谢
摘要：
鱼露风味浓郁、独特,是部分地区传统调味品。传统鱼露发酵周期过长及含盐量太高限制其发展;但低盐、速酿鱼露存在香气不足的缺陷。通过添加增香微生物加快鱼露香气形成或改善鱼露香气是提高鱼露品质的重要方法。本文综述了鱼露香气物质常用的检测、鉴定方法,归纳获得21种鱼露中最主要的特征香气物质,总结了10株改善鱼露香气的微生物,基于微生物氨基酸代谢途径,分析了鱼露部分特征香气物质产生机制,以期为鱼露品质改善提供参考。
语种：
中文
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鱼糜pH shifting工艺及其胶凝机制研究综述及展望
作者：
徐莉娜;贺海翔;罗煜;付湘晋;张琳;...
期刊：
食品工业科技 ,2018年39(11):301-306 ISSN：1002-0306
作者机构：
中南林业科技大学食品科学与工程学院, 湖南, 长沙, 410004;湖南宏兴隆湘莲食品有限公司, 湖南, 湘潭, 411200;[徐莉娜; 罗煜; 付湘晋; 张琳; 丁玉琴] 中南林业科技大学食品科学与工程学院, 湖南, 长沙, 410004;[贺海翔] 湖南宏兴隆湘莲食品有限公司, 湖南, 湘潭, 411200
关键词：
pH-shifting技术;鱼糜;低值鱼;胶凝;共混胶凝;微相分离
摘要：
鱼糜是我国低值淡水鱼深加工的主要研究方向之一,但由于低值淡水鱼个体小、刺多,现有工艺得率低,而pH-shifting技术能显著提高低值鱼鱼糜得率,且凝胶强度更好或相当。因此,该技术被认为是很有工业化前景的鱼糜加工新技术。本文综述了近年来的研究进展,包括pH-shifting工艺对鱼糜蛋白组成、鱼糜蛋白质分子结构、鱼糜胶凝特性、蛋白降解、蛋白交联及鱼糜凝胶微结构的影响。并提出以下观点:可采用蛋白质组学方法研究pH-shifting工艺对鱼糜蛋白质组成的影响,解析各蛋白组分在胶凝过程中的作用; pH-shifting鱼糜凝胶是蛋白聚集体与未变性蛋白的混合凝胶,可从共混胶凝、微相分离的角度研究pH-shifting鱼糜凝胶微结构形成机制。
语种：
中文
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Rice Bioactive Peptide Binding with TLR4 to Overcome H2O2-Induced Injury in Human Umbilical Vein Endothelial Cells through NF-κB Signaling
作者：
Liang, Ying;Lin, Qinlu* ;Huang, Ping;Wang, Yugian;Li, Jiajia;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2018年66(2):440-448 ISSN：0021-8561
通讯作者：
Lin, Qinlu
作者机构：
[Huang, Ping; Li, Jiajia; Cao, Jianzhong; Wang, Yugian; Zhang, Lin; Liang, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Mol Nutr Branch, Changsha 410004, Hunan, Peoples R China.;[Huang, Ping; Li, Jiajia; Cao, Jianzhong; Wang, Yugian; Zhang, Lin; Liang, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, 498 Shaoshan South Rd, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Lin, Qinlu] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, 498 Shaoshan South Rd, Changsha 410004, Hunan, Peoples R China.
关键词：
Antioxidants;Bins;Cell death;Cells;Cytology;Endothelial cells;Peptides;Proteins;Bioactive peptides;Cardio-vascular disease;Human umbilical vein endothelial cells;HUVECs;Molecular mechanism;Protective function;Reactive oxygen species;Toll-like receptor 4;Cell signaling;caspase 3;hydrogen peroxide;immunoglobulin enhancer binding protein;peptide;plant extract;toll like receptor 4;apoptosis;cardiovascular disease;chemistry;drug effects;genetics;human;metabolism;Oryza;oxidative stress;pathophysiology;signal transduction;umbilical vein endothelial cell;Apoptosis;Cardiovascular Diseases;Caspase 3;Human Umbilical Vein Endothelial Cells;Humans;Hydrogen Peroxide;NF-kappa B;Oryza;Oxidative Stress;Peptides;Plant Extracts;Signal Transduction;Toll-Like Receptor 4
摘要：
Reactive oxygen species-induced vessel endothelium injury is crucial in cardiovascular diseases progression. Rice-derived bran bioactive peptides (RBAP) might exert antioxidant effect through unknown mechanisms. Herein, we validated the antioxidant effect and mechanism of RBAP on H2O2-induced oxidative injury in human umbilical vein endothelial cells (HUVECs). Here, HUVECs were treated with RBAP under H2O2 stimulation; the effects of RBAP on HUVECs oxidative injury were evaluated. H2O2 injury-induced cell morphology changes were ameliorated by RBAP. The effect of H2O2- on HUVEC apoptosis (percentage of apoptotic cell: 38.00 ± 2.00 in H2O2 group vs 21.07 ± 2.06 in RBAP + H2O2 group, P = 0.0013 compared to H2O2 group), the protein levels of cleaved caspase-3 (relative protein expression: 2.90 ± 0.10 in H2O2 group vs 1.82 ± 0.09 in RBAP + H2O2 group, P < 0.0001 compared to H2O2 group) and p-p65 (relative protein expression: 1.86 ± 0.09 in H2O2 group vs 1.35 ± 0.08 in RBAP + H2O2 group, P < 0.0001 compared to H2O2 group) could be attenuated by RBAP. RBAP exerts its protective function through binding with Toll-like receptor 4 (TLR4). Taken together, RBAP protects HUVECs against H2O2-induced oxidant injury, which provided the theoretical basis for the molecular mechanism of rice deep processing and exploitation of functional peptides. © 2017 American Chemical Society.
语种：
英文
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Research Progress on Signaling Pathway-Associated Oxidative Stress in Endothelial Cells
作者：
Liang, Ying;Li, Jiajia;Lin, Qinlu* ;Huang, Ping;Zhang, Lin;...
期刊：
Oxidative Medicine and Cellular Longevity ,2017年2017:7156941 ISSN：1942-0900
通讯作者：
Lin, Qinlu
作者机构：
[Huang, Ping; Li, Jiajia; Wu, Wei; Ma, Youchu; Zhang, Lin; Liang, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Changsha 410004, Hunan, Peoples R China.;[Huang, Ping; Li, Jiajia; Wu, Wei; Ma, Youchu; Zhang, Lin; Liang, Ying; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Lin, Qinlu] C;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Deep Proc, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Cell signaling;Cells;Cytology;Diseases;Oxidative stress;Cardio-vascular disease;Scientific direction;Signal pathways;Signaling pathways;Endothelial cells;eNOS protein;hydroxymethylglutaryl coenzyme A reductase kinase;immunoglobulin enhancer binding protein;PI3K protein;protein;protein kinase B;unclassified drug;cell function;cell growth;endothelium cell;human;oxidation;oxidative stress;Review;signal transduction;animal;biological model;endothelium cell;metabolism;oxidation reduction reaction;pathology;Cells;Medicine;Animals;Endothelial Cells;Humans;Models, Biological;Oxidation-Reduction;Oxidative Stress;Signal Transduction
摘要：
Studying the mechanisms of oxidative stress in endothelial cells is vital to the discovery of novel drugs for the treatment of cardiovascular disease. This article reviews the progress within the field of the role of oxidative responses in the physiology and growth of endothelial cells and emphasizes the effects of several main signal pathways involved in the oxidative stress of endothelial cells. Herein, we aim to provide scientific direction that can serve as a basis for researchers specializing in the signaling pathway of oxidative stress. © 2017 Ying Liang et al.
语种：
英文
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Multi-Target Screening and Experimental Validation of Natural Products from Selaginella Plants against Alzheimer's Disease
作者：
Deng, Yin-Hua;Wang, Ning-Ning;Zou, Zhen-Xing;Zhang, Lin;Xu, Kang-Ping;...
期刊：
Frontiers in Pharmacology ,2017年8(AUG):539 ISSN：1663-9812
通讯作者：
Cao, Dong-Sheng;Tan, Gui-Shan
作者机构：
[Zou, Zhen-Xing; Tan, Gui-Shan; Wang, Ning-Ning; Chen, Alex F.; Cao, Dong-Sheng; Xu, Kang-Ping; Cao, DS; Tan, GS; Deng, Yin-Hua] Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;[Zou, Zhen-Xing; Tan, Gui-Shan] Cent South Univ, Xiangya Hosp, Pharm Dept, Changsha, Hunan, Peoples R China.;[Zhang, Lin] Cent South Univ Forestry & Technol, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China.;[Chen, Alex F.; Cao, Dong-Sheng] Cent South Univ, Xiangya Hosp 3, Ctr Vasc Dis & Translat Med, Changsha, Hunan, Peoples R China.
通讯机构：
[Cao, DS; Tan, GS; Tan, Gui-Shan; Cao, Dong-Sheng] C;Cent South Univ, Xiangya Sch Pharmaceut Sci, Changsha, Hunan, Peoples R China.;Cent South Univ, Xiangya Hosp, Pharm Dept, Changsha, Hunan, Peoples R China.;Cent South Univ, Xiangya Hosp 3, Ctr Vasc Dis & Translat Med, Changsha, Hunan, Peoples R China.
关键词：
Alzheimer;BACE1;MAO-B;Selaginella plants;multi-target SAR;multi-target screening
摘要：
Alzheimer's disease (AD) is a progressive and irreversible neurodegenerative disorder which is considered to be the most common cause of dementia. It has a greater impact not only on the learning and memory disturbances but also on social and economy. Currently, there are mainly single-target drugs for AD treatment but the complexity and multiple etiologies of AD make them difficult to obtain desirable therapeutic effects. Therefore, the choice of multi-target drugs will be a potential effective strategy inAD treatment. To find multi-target active ingredients for AD treatment from Selaginella plants, we firstly explored the behaviors effects on AD mice of total extracts (TE) from Selaginella doederleinii on by Morris water maze test and found that TE has a remarkable improvement on learning and memory function for AD mice. And then, multi-target SAR models associated with AD-related proteins were built based on Random Forest (RF) and different descriptors to preliminarily screen potential active ingredients from Selaginella. Considering the prediction outputs and the quantity of existing compounds in our laboratory, 13 compounds were chosen to carry out the in vitro enzyme inhibitory experiments and 4 compounds with BACE1/MAO-B dual inhibitory activity were determined. Finally, the molecular docking was applied to verify the prediction results and enzyme inhibitory experiments. Based on these study and validation processes, we explored a new strategy to improve the efficiency of active ingredients screening based on trace amount of natural product and numbers of targets and found some multi-target compounds with biological activity for the development of novel drugs for AD treatment. © 2017 Deng, Wang, Zou, Zhang, Xu, Chen, Cao and Tan.
语种：
英文
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Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions
作者：
Yang, Tao;Zhu, Fang;Zhou, Tianxiao;Cao, Jianzhong;Xie, Yibo;...
期刊：
Analytical Sciences ,2017年33(2):191-196 ISSN：0910-6340
通讯作者：
Zhang, Lin
作者机构：
[Zhou, Tianxiao; Yang, Tao; Zhu, Fang; Cao, Jianzhong; Zhang, Meiyi; Xie, Yibo; Zhang, Lin; Lin, Qinlu; Wang, Yan] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China.;[Zhou, Tianxiao; Yang, Tao; Zhu, Fang; Cao, Jianzhong; Zhang, Meiyi; Xie, Yibo; Zhang, Lin; Lin, Qinlu; Wang, Yan] Cent South Univ Forestry & Technol, Dept Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Cao, Dong-Sheng] Cent S Univ, Sch Pharmaceut Sci, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Further Proc, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Dept Food Sci & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
Copper ion;determination;fluorescent peptide probe;selectivity;water sample
摘要：
This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu 2+ ) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu 2+ via quenching of the probe fluorescence upon a binding of Cu 2+ . The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu 2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu 2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu 2+ in aqueous solutions. © 2017 The Japan Society for Analytical Chemistry.
语种：
英文
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