期刊论文

Extraction of Polysaccharides from Dendrobium officinale and Their Inhibition Effect on Hydroxyl Radical-Induced Apoptosis of SH-SY5Y Cells

中文

食品科学

1002-6630

2020

41

14

286-293

10.7506/spkx1002-6630-20190802-033

国家自然科学基金青年科学基金项目(81501619) 湖南省科技创新平台与人才计划项目(2017TP1021) 湖南省自然科学基金青年项目(2018JJ3300) 中南林业科技大学大学生研究性学习和创新性实验计划项目(2017)。

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以酶解超声提取法提取铁皮石斛多糖,通过对料液比、超声时间、复合酶质量分数的单因素试验和正交试验,确定铁皮石斛多糖的最佳提取条件;以高效液相色谱法对铁皮石斛多糖进行组成成分分析;以香豆素-3-羧酸荧光法测定铁皮石斛多糖的羟自由基清除能力;以噻唑蓝实验和细胞流式实验研究铁皮石斛多糖提取物对SH-SY5Y细胞的保护作用。结果表明,最优铁皮石斛多糖提取条件为料液比1∶100(g/mL)、超声时间2 h、复合酶(纤维素酶和果胶酶1∶1等质量混合)质量分数50%,最优提取条件下铁皮石斛多糖得率为25.69%,其主要单糖组分为甘露糖和葡萄糖。在质量浓度为20~80 μg/mL范围内铁皮石斛多糖提取物能有效清除Cu...

The ultrasonic-assisted enzymatic extraction of polysaccharides from Dendrobium officinale was optimized using one-factor-at-a-time and orthogonal array design methods. Solid-to-liquid ratio, ultrasonication time, and enzyme concentration were considered as independent variables. High performance liquid chromatography (HPLC) was used for analyzing the monosaccharide composition of D. officinale polysaccharides. Coumarin-3-carboxylic acid (CCA) fluorescence method was employed for determining the antioxidant activity of D. officinale polysacchar...