期刊论文

Fmoc-FF polypeptide hybrid hydrogel for lactase controlled-release

中文

粮食与油脂

1008-9578

2020

33

3

86-90

10.3969/j.issn.1008-9578.2020.03.024

长沙市科技计划重点项目(kq1907096) 湖南省科技创新平台与人才计划项目(2017TP1021) 湖南省食品药品监督管理局食品药品安全科技项目(湘食药科201702) 湖南省自然科学基金面上项目(2018JJ2232) 中南林业科技大学大学生研究性学习和创新性实验计划项目(2017)。

本校为第一机构

探讨Fmoc-FF （Phe）多肽与卡拉胶形成的凝胶对乳糖酶在模拟胃液中的保护作用以及对包埋物的控制释放作用。结果表明:在Fmoc-FF与卡拉胶的质量比为1∶15、卡拉胶的质量浓度为48%时,经模拟胃肠道后,杂化水凝胶包埋的乳糖酶的酶活可以达到未经过胃肠道处理的酶活的67.63%,远高于同样实验条件下未经包埋的乳糖酶的酶活（0%）。因此,Fmoc-FF/卡拉胶杂化水凝胶能有效保护乳糖酶不被胃液破坏,且对包埋物有控制释放的作用,可作为口服乳糖酶的控释包埋材料。

The protective and controlled release effect of Fmoc-FF(Phe) polypeptide/carrageenan hydrogel on lactase in simulated gastric juice were exploved. The results showed when the mass ratio of Fmoc-FF to carrageenan was 1 ∶ 15, the mass concentration of carrageenan was 48 %, the activity of lactase embedded in the hybrid hydrogel could reach 67.63 % of the enzyme which was not treated by gastrointestinal tract, which was much higher than that of the unencapsulated lactase(0 %) under the same experimental conditions. Therefore, Fmoc-FF/carrageenan ...