期刊论文

Study on inhibiting effect of orange peel flavone extract on apoptosis of SH-SY5Y cells induced by hydroxyl radical

中文

粮食与油脂

1008-9578

2021

34

01

123-127

10.3969/j.issn.1008-9578.2021.01.032

本校为第一机构

以酶解超声提取法提取橘皮黄酮,通过单因素和正交试验研究纤维素酶质量浓度、超声时间、乙醇体积浓度、料液比对黄酮得率的影响,确定橘皮黄酮的最佳提取条件:纤维素酶质量浓度0.5 g/mL、超声时间60 min、乙醇体积浓度90%、料液比1∶60（g/mL）。以香豆素-3-羧酸（Coumarin-3-carboxylic acid,CCA）荧光法和噻唑蓝（Methyl thiazoly1 tetrazolium,MTT）试验发现橘皮黄酮在浓度为20～100μg/mL范围内能有效清除Cu2+催化H2O2及抗坏血酸产生的羟基自由基并有效抑制SH-SY5Y细胞的凋亡,且其作用效果呈浓度依赖性。

Orange peel flavonoids were extracted by enzymatic hydrolysis ultrasonic extraction method.The effects of cellulase concentration,ultrasonic time,ethanol concentration,ratio of material to liquid on yield of flavone were determined. The optimal extraction conditions of orange peel flavone were as follows: cellulase concentration 0. 5 g/mL,ultrasonic time 60 min,volume concentration of ethanol 90 %,and material-to-liquid ratio 1∶60 (g/mL). Using coumarin-3-carboxylic acid (CCA) fluorescence method and methyl thiazoly1 tetrazolium (MTT) assay,it...