作者：
Bo Zhou;Dan Peng;XiaoFeng Tan;DeYi Yuan;XuanMing Liu;...
期刊：
Protein Expression and Purification,2015年109:127 ISSN：1046-5928
通讯作者：
Lin Zhang
作者机构：
College of Bioscience and Biotechnology of Center South University of Forestry and Technology, 410004 Changsha, China;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410004 Changsha, China;Key Laboratory of Cultivation and Protection for Non-Wood Forest Tree, Central South University of Forestry and Technology, 410004 Changsha, China;Collaborative Innovation Center of Cultivation and Utilization for Non-Wood Forest Tree, Central South University of Forestry and Technology, 410004 Changsha, China;[XuanMing Liu] Key Laboratory of Plant Function Gnomonic for Development and Regulation, Hunan University, 410082 Changsha, China
通讯机构：
[Lin Zhang] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, 410004 Changsha, China<&wdkj&>Key Laboratory of Cultivation and Protection for Non-Wood Forest Tree, Central South University of Forestry and Technology, 410004 Changsha, China<&wdkj&>Collaborative Innovation Center of Cultivation and Utilization for Non-Wood Forest Tree, Central South University of Forestry and Technology, 410004 Changsha, China
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2015年63(3):929-942 ISSN：0021-8561
通讯作者：
Cao, Heping
作者机构：
[Yuan, De-Yi; Zou, Feng; Fan, Xiaoming; Zhang, Lin; Lin, Qing] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Feng, Yanzhi] China Paulownia Res Ctr, Zhengzhou 450003, Henan Province, Peoples R China.;[Zeng, Xiaochun; Cao, Heping] ARS, So Reg Res Ctr, USDA, New Orleans, LA 70124 USA.;[Cao, Heping] ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA.
通讯机构：
[Cao, Heping] A;ARS, So Reg Res Ctr, USDA, 1100 Robert E Lee Blvd, New Orleans, LA 70124 USA.
作者机构：
[卢锟; 李泽; 龙洪旭; Lü J.-B.; 林青; 谭晓风; 张琳] Key Laboratory of Cultivation and Protection for Non- Wood Forest Trees, Ministry of Education, Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
[Tan, X.-F.] K;Key Laboratory of Cultivation and Protection for Non- Wood Forest Trees, Ministry of Education, Cooperative Innovation Center of Cultivation and Utilization for Non-Wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
摘要：
Fructose 1,6-bisphosphate aldolase (FBA) catalyzes the reverse cleavage of fructose-1,6-bisphosphate into dihydroxyacetone phosphate (DHAP) and 3-phosphate glyceraldehyde (G-3-P). DHAP acts as a precursor for the synthesis of glycerol, an important osmotically compatible solute under salt stress. Using rapid amplification of cDNA ends, the complete sequence of FBA1 cDNA was obtained from Camellia oleifera, a tree originated from China and notable as an important source of edible oil obtained from its seeds. Camellia oleifera FBA1 (CoFBA1) cDNA has a total length of 1,697-bp with 1,179-bp ORF, flanked by 250-bp 5′-UTR and 246-bp 3′-UTR. It was predicted to encode a 392 amino acid protein with a calculated molecular mass of 42.72 kDa and theoretical pI of 8.39. Real-time PCR analysis indicated that the CoFBA1 gene was strongly expressed in stems, weakly in roots, and induced under salt stress to a threshold before dropping at high salt concentrations (12 g L−1 NaCl). Over-expression of recombinant CoFBA1 resulted in increased tolerance to salinity in transgenic Brassica napus plants, which grew better at low salt concentrations than on media without NaCl. At all salt concentrations, the transgenic plants showed improved growth parameters (stem and root lengths and germination rates) in comparison with wild-type plants. These findings suggest that CoFBA1 plays very important roles in salt stress response, improving the survival ability of C. oleifera under salt stress conditions.
摘要：
In this report, self-sterility in Camellia oleifera was explored by comparing structural and statistical characteristics following self-pollination (SP) and cross-pollination (CP). Although slightly delayed pollen germination and pollen tube growth in selfed ovaries compared to crossed ovaries was observed, there was no significant difference in the percentages of pollen that germinated and pollen tubes that grew to the base of the style. There was also no difference in morphological structure after the two pollination treatments. However, the proportions of ovule penetration and double fertilization in selfed ovules were significantly lower than in crossed ovules, indicating that a prezygotic late-acting self-incompatible mechanism may exist in C. oleifera. Callose deposition was observed in selfed abortive ovules, but not in normal. Ovules did not show differences in anatomic structure during embryonic development, whereas significant differences were observed in the final fruit and seed set. In addition, aborted ovules in selfed ovaries occurred within 35 days after SP and prior to zygote division. However, this process did not occur continuously throughout the life cycle, and no zygotes were observed in the selfed abortive ovules. These results indicated that the self-sterility in C. oleifera may be caused by prezygotic late-acting self-incompatibility (LSI).
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
摘要：
Oil-tea tree is one of the most important woody edible oil plants; however,
lack of useful molecular markers hinders current genetic research. We
performed transcriptome sequencing of developing seeds and characterized
microsatellites from transcriptome sequences to identify valuable markers for
C. oleifera molecular genetics research. A total of 69,798 unigenes were
identified, in which 6,949 putative SSR motifs from 6,042 SSR-containing
unique putative transcripts were discovered. Twenty-nine primer pairs
corresponding to 29 unigene loci were designed, of which 15 polymorphic
genic-SSR markers were developed in 18 varieties and characterized by
capillary electrophoresis. The number of alleles per locus (Na) ranged from 2
to 14, the expected heterozygosity (He) ranged from 0.374 to 0.876, and the
polymorphism information content (PIC) values ranged from 0.498 to 0.887,
respectively. Cross-species amplification was also conducted in 15 varieties
of C. japonica. All 15 markers successfully amplified PCR products with
expected size in C. japonica and exhibited polymorphisms. The 15 polymorphic
genic- SSR markers will have potential for applications in genetic diversity
evaluation, molecular fingerprinting identification, comparative genome
analysis, and genetic mapping in the C. oleifera and C. japonica.
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
