期刊论文

中文

植物遗传资源学报

1672-1810

2015

16

3

603-611

10.13430/j.cnki.jpgr.2015.03.025

(2013BAD14B04):国家“十二五”科技支撑计划课题

本校为第一机构

本研究采用高通量测序技术对淀粉积累高峰期的锥栗种仁进行转录组测序分析,对得到的Unigene进行功能注释、分类及代谢通路分析,并进一步通过实时定量PCR方法分析了7个与淀粉和蔗糖代谢相关酶基因在锥栗种仁发育过程中的表达特征。结果显示:de nvo组装后共获得53629条Unigene序列,序列平均长度为746 bp;通过与其他核酸、蛋白质数据库的Blast搜索比对,共26739条Unigene序列获得基因注释,占All-Unigene的49.86%;与COG数据库比对后将其注释的14413条Unigene序列划分成25类;GO功能注释的33926条Unigene基因共分成细胞组分、分子功能和生物过程3大类58个分支;与KEGG数据库比对结果注释的5277条Unigene...

Castanea henryi is an important woody grain plants in south of China. The current research on the fruit development of C. henryi mainly focuses on fruit component analysis, fruit quality analysis, and fruit processing,whereas the molecular mechanisms involved in fruit development remain to be uncovered. Transcriptome sequencing and analysis were conducted on kernel in the beginning of starch synthesis peak phase of C. henryi using Illumina platform-based strategy. Expression patterns of seven starch and sucrose metabolism-related Unigenes were ...