期刊论文

中文

园艺学报

0513-353X

2015

42

12

2341-2352

10.16420/j.issn.0513-353x.2015-0399

国家自然科学基金项目（31272124）；

本校为其他机构

利用东方梨中已鉴定的52个S等位基因HV区c DNA序列作为靶基因序列设计探针,制备梨S基因c DNA检测芯片,每张芯片上含有240个位点55个c DNA探针,包含所有序列完善的S基因HV区特异的c DNA序列。以被检测品种雌蕊c DNA为模板,采用Cy3荧光修饰引物经S基因特异PCR扩增标记被检测品种的c DNA序列,并与芯片杂交以检测不同品种的S基因型。结果表明:利用c DNA检测芯片与‘丽江黄酸梨’、‘秀玉’、‘弥渡玉梨’、‘白面梨’和‘德胜香’等已知S基因型品种杂交,杂交结果显示与S基因寡核苷酸芯片检测信号一致,与各品种已知S基因型相符合。利用c DNA芯片和进一步完善的S基因寡核苷酸芯片并行检测鉴定了‘文山...

Using the c DNA sequences from Hyper variable（HV）regions of 52 S-alleles in Oriental pear cultivars,c DNA microarray for S-RNase detections was established. Totally 240 dots and 55 c DNA probes,which includes all the HV specific c DNA sequences of perfected S-RNase gene,were spotted on the chip. By Cy3-labeled primers and c DNA template of tested cultivars pistils,the Cy3-labeled specific c DNA PCR products of S-RNase were amplified and hybridized with the c DNA microarray in order to detect the S genotype of pear cultivars. Cultivars with kn...