Development of 15 Genic-SSR markers in oil-tea tree (camellia oleifera) based on transcriptome sequencing

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成果类型：

期刊论文

作者：

Jia, Baoguang;Lin, Qing;Zhang, Lin*;Tan, Xiaofeng;Lei, Xiaolin;...

通讯作者：

Zhang, Lin

作者机构：

[Shao, Fenggong; Jia, Baoguang; Tan, Xiaofeng; Zhang, Lin; Lin, Qing] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha, Hunan, Peoples R China.

[Shao, Fenggong; Jia, Baoguang; Tan, Xiaofeng; Zhang, Lin; Lin, Qing] Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilizat Nonwood, Changsha, Hunan, Peoples R China.

[Lei, Xiaolin] Jiangxi Acad Forestry, Nanchang, Peoples R China.

[Hu, Xiaoyi] Anhui Agr Univ, Sch Forestry & Landscape Architecture, Hefei, Peoples R China.

[Zhang, Lin] 498 South Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

通讯机构：

[Zhang, Lin] 4

498 South Shaoshan Rd, Changsha 410004, Hunan, Peoples R China.

语种：

英文

关键词：

Camellia oleifera;Cross-species amplification;Genic-SSR;Transcriptome sequencing;Unigene

期刊：

Genetika

ISSN：

0534-0012

年：

2014

卷：

期：

页码：

789-797

DOI：

10.2298/GENSR1403789J

基金类别：

"948" Project of China State Forestry Administration [2012-4-42]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31100497]; Chinese Ministry of EducationMinistry of Education, China [212126]

机构署名：

本校为第一机构

院系归属：

林学院

摘要：

Oil-tea tree is one of the most important woody edible oil plants; however, lack of useful molecular markers hinders current genetic research. We performed transcriptome sequencing of developing seeds and characterized microsatellites from transcriptome sequences to identify valuable markers for C. oleifera molecular genetics research. A total of 69,798 unigenes were identified, in which 6,949 putative SSR motifs from 6,042 SSR-containing unique putative transcripts were discovered. Twenty-nine primer pairs corresponding to 29 unigene loci were designed, of which 15 polymorphic genic-S...

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Development of 15 Genic-SSR markers in oil-tea tree (camellia oleifera) based on transcriptome sequencing

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