Development and cross-species transferability of unigene-derived microsatellite markers in an edible oil woody plant, Camellia oleifera (Theaceae)

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成果类型：

期刊论文

作者：

Jia, B. G.;Lin, Q.;Feng, Y. Z.;Hu, X. Y.;Tan, X. F.;...

通讯作者：

Zhang, L.

作者机构：

[Shao, F. G.; Jia, B. G.; Zhang, L.; Lin, Q.; Tan, X. F.] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.

[Shao, F. G.; Jia, B. G.; Zhang, L.; Lin, Q.; Tan, X. F.] Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilizat Nonwood, Changsha, Hunan, Peoples R China.

[Feng, Y. Z.] China Paulownia Res Ctr, Zhengzhou, Henan, Peoples R China.

[Hu, X. Y.] Anhui Agr Univ, Sch Forestry & Landscape Architecture, Hefei, Anhui, Peoples R China.

通讯机构：

[Zhang, L.] C

Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.

语种：

英文

关键词：

C. chekangoleosa;C. japonica;Camellia oleifera;Cross-species transferability;Microsatellite;Unigene

期刊：

Genetics and Molecular Research

ISSN：

1676-5680

年：

2015

卷：

期：

页码：

6906-6916

DOI：

10.4238/2015.June.18.33

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31100497]; Chinese Ministry of EducationMinistry of Education, China [212126]

机构署名：

本校为第一且通讯机构

院系归属：

林学院

摘要：

Camellia oleifera is an important edible oil woody plant in China. Lack of useful molecular markers hinders current genetic research on this tree species. Transcriptome sequencing of developing C. oleifera seeds generated 69,798 unigenes. A total of 6949 putative microsatellites were discovered among 6042 SSR-containing unigenes. Then, 150 simple sequence repeats (SSRs) were evaluated in 20 varieties of C. oleifera. Of these, 52 SSRs revealed polymorphism, with the number of alleles per locus ranging from 2 to 15 and expected heterozygosity val...

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Development and cross-species transferability of unigene-derived microsatellite markers in an edible oil woody plant, Camellia oleifera (Theaceae)

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