期刊论文

Zhang, L.

中文

植物生理与分子生物学学报

1671-3877

2014

50

10

1608-1612

10.13592/j.cnki.ppj.2014.0317

(2012-4-42):资助国家“948”引进项目“木本植物高效遗传转化及外源基因清除技术引进”

本校为第一且通讯机构

以油桐无菌苗叶柄为外植体,研究植物生长调节剂对其离体培养及植株再生的影响。结果表明:叶柄直接诱导不定芽的最佳培养基1/2MS+3.0 mg·L~(-1)6-BA+0.05 mg·L~(-1) IAA,诱导率达91.67%;最佳继代增殖培养基1/2MS+3.0 mg·L~(-1)6-BA+0.05 mg·L~(-1) IBA+1.0 mg·L~(-1) GA_3,增殖系数可达4.89;最佳生根培养基为1/2MS+0.05 mg·L~(-1) IBA,生根率96.18%。炼苗移栽到泥炭土:珍珠岩:黄土=2:1:1的基质中,成活率达93.55%以上。

The effects of plant growth regulators on plant regeneration in vitro were studied using germ-free petioles of tung tree (Vernicia fordii) as explants. The results showed that the fittest medium for shoot differentiation of petioles was 1/2MS+3.0 mg·L-1 6-BA+0.05 mg·L-1 IAA, with a 91.67% shoot induction rate; the best medium for shoot multiplication was 1/2MS+3.0 mg·L-1 6-BA+0.05 mg·L-1 IBA+1.0 mg·L-1 GA3, with the multiplication coefficient reaching 4.89; the best rooting medium was 1/2MS+0.05 mg·L-1 IBA, with a 96.18% rooting rate. Aft...