期刊论文

中文

中南林业科技大学学报

1673-923X

2015

35

6

93-97

10.14067/j.cnki.1673-923x.2015.06.017

国家“十二五”农村领域科技计划课题（2013BAD14B03）；

本校为第一机构

运用 Illumina 测序平台的 RNA-seq 技术对中秋酥脆枣的花、果实和枣吊进行了转录组测序，并对测序获得的 Unigene 进行微卫星特征分析。经序列组装和拼接，共获得 34 587 个 Unigene，运用 Misa 软件分析发现12 624 个微卫星。在所得转录组序列的单碱基至五碱基微卫星中，以单碱基微卫星最多（6 314 个，50.02%），并以 A/T（6 217 个，98.46%）为主要重复单元；二碱基微卫星（3 335 个，26.42%）次之，其中以 AG/CT（2532 个，75.92%）类型最多；再次是三碱基微卫星（2 871 个，22.74%）；最后是四碱基和五碱基微卫星，二者仅占所有微卫星信息的 0.82%。单碱基微卫星所占比例最多，为枣最优势...

Transcriptome sequencing was conducted on fruits, flowers, and bearing shoots of elite Ziziphus jujuba cv. ＇Zhongqiusucui＇ by using Illumina-based RNA-seq technology. The microsatellites characteristics were then analyzed from the obtained unigenes. The assembled unigenes were totally 34 584, from which 12 624 microsatellites repeats were detected with Misa sottware. In the 1 - 5 bases repeat microsatellites, the mononucleotide repeat microsatellites （MNRs） were the maximum in quantitative terms （6 314, 50.02%）, of which the A/T （6 217, ...