期刊论文

中文

林业科学

1001-7488

2014

50

8

68-75

10.11707/j.1001-7488.20140810

国家自然科学基金面上项目（31070603） 国家自然科学基金项目（31100497） 湖南省科技计划项目（2012NK3065）.

本校为第一机构

以油茶果实膨大期和油脂合成高峰期的种子为材料,进行转录组测序和表达谱数据库构建,并对油茶种子α-亚麻酸代谢途径相关基因进行分析。转录组测序获得油茶种子α-亚麻酸代谢非冗余基因Unigenes序列共112条,与α-亚麻酸代谢密切相关的不饱和脂肪酸合成途径非冗余基因Unigenes序列94条,包含12种调控α-亚麻酸代谢主要酶基因。表达谱数据分析显示,果实膨大期和油脂合成高峰期的α-亚麻酸合成代谢途径相关基因具有不同的表达模式,参与油茶种子α-亚麻酸的合成及形成多种不饱和脂肪酸的物质转化。采用实时荧光定量PCR方法验证油茶种子LOX,AOS,ACOX,AOC,OPR,AACT,PLA2,HPL,DOX,MFP,FAD2,FAD3等关键基...

In an effort to better understand the factors that regulate α-linolenic acid metabolism in Camellia oleifera seeds,the transcriptome library and expression profile of C. oleifera seeds both in the fruit expanding period and at the peak stage of lipid biosynthesis have been constructed and the functional genes related to α-linolenic acid metabolism have been analyzed in detail. Study on transcriptome showed that there were one hundred and twelve non-redundant gene unigenes related to α-linolenic acid metabolism and ninety-four unigenes relate...