作者：
Huang, Huilian;Sun, Jianghao;Mccoy, Joe-Ann;Zhong, Haiyan;Fletcher, Edward J.;...
期刊：
SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY,2015年105:121-129 ISSN：0584-8547
通讯作者：
Harnly, James
作者机构：
[Harnly, James; Huang, Huilian; Chen, Pei; Sun, Jianghao] USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA.;[Huang, Huilian] Jiangxi Univ Tradit Chinese Med, TCM, Key Lab Modern Preparat, Minist Educ, Nanchang, Jiangxi, Peoples R China.;[Mccoy, Joe-Ann] North Carolina Arboretum Germplasrn Repository, Asheville, NC USA.;[Zhong, Haiyan] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha, Hunan, Peoples R China.;[Fletcher, Edward J.] Strategic Sourcing Inc, Banner Elk, NC 28604 USA.
通讯机构：
[Harnly, James] U;USDA ARS, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA.
关键词：
Black cohosh;Mass spectrometer;Fingerprinting;PCA
摘要：
Flow injection mass spectrometry (FIMS) was used to provide chemical fingerprints of black cohosh (Actaea racemosa L.) in a manner of minutes by omitting the separation step. This method has proven to be a powerful tool for botanical authentication and in this study it was used to distinguish between three Actaea species prior to a more detailed chemical analysis using ultra high-performance liquid chromatography high-resolution mass spectrometry (UHPLC-HRMS). Black cohosh has become increasingly popular as a dietary supplement in the United States for the treatment of symptoms related to menopause. However, it has been known to be adulterated with the Asian Actaea dahurica (Turcz. ex Fisch. & C.A.Mey.) Franch. species (syn. Cimicifuga dahurica (Turcz.) Maxim). Existing methods for identification of black cohosh and differentiation of Actaea species are usually lengthy, laborious, and lack robustness, often based on the comparison of a few pre-selected components. Chemical fingerprints were obtained for 77 black cohosh samples and their related species using FIMS in the negative ion mode. The analysis time for each sample was less than 2 mm, All data were processed using principal component analysis (PCA). FIMS fingerprints could readily differentiate all three species. Representative samples from each of the three species were further examined using UHPLC-MS to provide detailed profiles of the chemical differences between the three species and were compared to the PCA loadings. This study demonstrates a simple, fast, and easy analytical method that can be used to differentiate A. racemosa, Actaea podocarpa, and A. dahurica. (C) 2014 Published by Elsevier B.V.
作者机构：
[钟海雁; 曹清明] Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Central South University of Forestry and Technology), Ministry of Education, Changsha, China;[钟海雁; 邬靖宇; 曹清明] Faculty of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, China
作者机构：
[钟海雁; 曹清明; 周波; 朱勇; 龙奇志] Central South University of Forestry and Technology Faculty of Food Science and Engineering, Changsha 410004, China;[钟海雁; 曹清明; 周波; 龙奇志] Hunan Key Laboratory of Deeply Processing and Quality Control of Cereals and Oils, Changsha 410004, China
通讯机构：
Central South University of Forestry and Technology Faculty of Food Science and Engineering, China
作者机构：
[钟海雁; 王进英] Faculty of Food Science and Engineering Central South University of Forestry and Technology, Changsha , China;[钟海雁; 王进英] Hunan key Laboratory of Deeply Processing and Quality Control of Cereals and Oils, Changsha, China
通讯机构：
Faculty of Food Science and Engineering Central South University of Forestry and Technology, Changsha, China
