期刊论文

Zhong, Haiyan

中文

中国粮油学报

1003-0174

2014

29

9

107-111

基金项目：国家自然科学基金（31070612）

本校为第一且通讯机构

建立了反相高效液相色谱-内标法测定茶油中7种酚类物质的含量。以丁香酸为内标物,C18反相柱(4.6 mm × 250 mm,5 μm)为分析柱,甲醇-水(0. 2% )为流动相,流速1. 0 mL/min;检测波长280 nm,柱温25 ℃。7种酚类物质在各自的线性范围内具有良好的线性关系,相关系数为0. 998 ~ 0. 999,检出限为0.008~0.028 μg/g。7种酚类在茶油中的平均回收率为76.783%~88.956%。结果表明,在选定的7中酚类物质中茶油只含有其中的4种,分别是没食子酸、儿茶素、4-羟基苯乙酸、槲皮素,而且这4种物质在8个油样中的含量各异,这可能与茶油的加工方法和品种有关。该方法准确,适用性好,可用于茶油中酚类物质的定性与定量分析...

The determination method for content of phenol compounds in camellia oil has been established by reverse phase high performance liquid chromatography （ RP - HPLC ） adopting syringic acid as internal standard. The RP- HPLC was conducted on C18 reverse phase column （24.6 mm ×250 mm,5 μm） at 25 ℃ with methanol -water （0.2%） as mobile phase. The flow rate of mobile phase was 1.0 mL/min. The detective wave- length was set up as 280 nm and column temperature at 25 ℃. It was proved that seven selected phenolic compounds had good linear relat...