期刊论文

Compounds and structure identiifcation in extracts from Camellia oleifera leaves by HPLC preparation

中文

经济林研究

1003-8981

2015

3

68-74

10.14067/j.cnki.1003-8981.2015.03.012

国家自科科学基金项目“高抗氧活性油茶生物酚组分的结构表征及其作用机理研究”（31070612/C161103）；

本校为第一机构

为了综合开发利用油茶资源,对油茶叶提取物的组分进行了制备和鉴定。将经过大孔树脂和柱分离纯化收集的组分II、III和VIII等油茶叶提取物3个组分进行了HPLC制备,得到了8个纯度较高的化合物,通过薄层显色、高清晰质谱和NMR,鉴定了其中4个化合物为：二氢白藜芦醇（1）、槲皮素（3）、槲皮素-3-鼠李糖苷（6）、1-（3′,5′-二甲氧基）苯基2-[4″-O-β-D-吡喃葡萄糖基（6→1）-O-α-L-吡喃鼠李糖基]苯乙烷（8）,其中槲皮素和槲皮素-3-鼠李糖苷是油茶中最常见的2个黄酮类物质,1-（3′,5′-二甲氧基）苯基2-[4′′-O-β-D-吡喃葡萄糖基（6→1）-O-α-L-吡喃鼠李糖基]苯乙烷为第2次报道,但提取率远高于...

In order to comprehensively develop and utilize Camellia oleifera resources, compounds in extracts from C. oleifera leaves were separated and identified. Three extracted parts of II, III and VIII from C. oleifera leaves were separated, purified and collected by using macroporous resin and column chromatography, and eight high-purity compounds were achieved by HPLC preparation. Four compounds were identified by methods of TLC colouring, high resolution LC-MS, and NMR, including dihydroresveratrol (1), quercetin (3), quercetin 3-O-β-D-rhamnoside...