摘要：
This research proposed a green and efficient enzyme-assisted steam distillation (EASD) method for extracting essential oils from Cinnamomum camphora leaves, and a novel neural network model was constructed to precisely optimize the extraction process parameters. The results demonstrated that the artificial neural network and genetic algorithm (GA-ANN) model exhibited higher accuracy and reliability than traditional models. The optimized conditions were as follows: distillation time 4.0 h, liquid–solid ratio 13.469 mL/g, incubation time 1.511 h, and cellulase dosage 0.555 g. The actual yield of the essential oils reached 1.365%, which was in accordance with the predicted yield of 1.373%. The yield was significantly higher than that obtained by steam distillation and predicted by other optimization methods. Moreover, the extraction kinetics analysis showed that first-order kinetics was a more suitable description of essential oils extraction. The extraction mechanism was clearly explained through the analysis of the extraction kinetics and microstructure. The enzyme exhibited a strong destructive effect on the plant cell wall, reducing mass transfer resistance and facilitating the dissolution of essential oils. Compared to steam distillation (SD) method, the EASD method notably diminished distillation time and energy consumption, while maintaining antioxidant activity.
期刊：
Sustainable Chemistry and Pharmacy,2024年39:101570 ISSN：2352-5541
通讯作者：
Li, XZ
作者机构：
[Lu, Ying; Li, Xiangzhou; Zhou, Peng; Li, XZ] Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Jiang, Zhi] Hunan Prima Drug Res Ctr Co Ltd, Hunan Key Lab Pharmacodynam & Safety Evaluat New D, Changsha 410329, Hunan, Peoples R China.;[Shen, Liqun] Guangxi Minzu Univ, Guangxi Collaborat Innovat Ctr Chem & Engn Forest, Key Lab Chem & Engn Forest Prod,State Ethn Affairs, Guangxi Key Lab Chem & Engn Forest Prod, Nanning 530006, Guangxi, Peoples R China.
通讯机构：
[Li, XZ ] C;Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Citrus aurantium L. var. amara Engl.;Pectin;Essential oil;Machine learning
摘要：
A green and efficient approach for the co-extraction of essential oil and pectin from Citrus aurantium L. var. amara Engl. was achieved by combining deep eutectic solvents (DES) with steam distillation. The optimal technological parameters were precisely identified through single-factor, response surface, and machine learning, with the DES concentration of 11.2%, the liquid-solid ratio of 10.6 mL/g, and the distillation time of 56.0 min. Under these conditions, the essential oil yield was 1.33 +/- 0.07%, and pectin yield was 14.50 +/- 0.58%. This process significantly increased the essential oil yield, reduced the solvent dosage, and shortened the distillation time, demonstrating remarkable progressiveness. Meanwhile, the extraction mechanism was clarified by observing the changes in the surface appearance and crystallinity of the raw material during the co-extraction process, and by calculating the interactions at the molecular level via density functional theory. The results confirmed that the ability of DES to dissolve pectin was much greater than that of water, which facilitated the breakdown of cell walls and accelerated the outward diffusion of essential oil from within the plant. Furthermore, the compositions of essential oil and pectin were identified. This research presents an environmentally friendly and efficient strategy for extracting essential oil and pectin from plant flowers, promoting the sustainable growth of the plant extraction industry.
期刊：
International Journal of Biological Macromolecules,2024年277(Pt 4):134215 ISSN：0141-8130
作者机构：
[Yang, Hongxiang; Li, Xiangzhou; Zhou, Peng] College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan, China;[Li, Xiangzhou] Institute of Natural Products Research and Development, Central South University of Forestry and Technology, Changsha 410004, Hunan, China. Electronic address: rlxz@163.com;[Shen, Liqun] Key Laboratory of Chemistry and Engineering of Forest Products, State Ethnic Affairs Commission, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning 530006, Guangxi, China
摘要：
The utilization and economic benefits of biomass resources can be maximized through rational design and process optimization. In this study, an innovative approach for the simultaneous extraction of essential oil and lignin from Cinnamomum camphora leaves by deep eutectic solvent (DES) and optimization of the process parameters was achieved using deep learning tools. With the water content of 40%, liquid-solid ratio of 9.00mL/g, and distillation time of 51.81min, the yields of the essential oil and lignin reached 3.15±0.02% and 9.75±0.15%, respectively. Notably, the efficiency of simultaneous extraction of essential oil improved by 23% compared to that of traditional steam distillation. Moreover, the extraction mechanism of the process was clarified. The connection between lignin with cellulose and hemicellulose was disintegrated by the DES, resulting in lignin shedding and hence accelerating the dissolution of essential oil. Moreover, the compositions of lignin and essential oil were also identified.
摘要：
<jats:p>Keggin-type phosphotungstic acid (HPW) supported on imidazolyl-activated carbon (AC-COIMI-HPW) catalysts was prepared, which was used to catalyze the oxidation of benzyl alcohol with aqueous H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>. In the presence of AC-COIMI-HPW, the benzyl alcohol conversion of 90.2% with 91.8% selectivity of benzaldehyde was obtained at 90°C for 6h in an acetonitrile aqueous solution. The catalyst exhibited an outstanding performance for the oxidation of various benzyl alcohols and aliphatic alcohols. In addition, the catalyst could be easily recovered and reused five times without significant deactivation. The characterization results showed that HPW was chemically bonded on the surface of the carbon material through an ionic bond. It is proposed that the combination of the imidazole cation with the HPW anion could not only tune the redox catalytic properties of the PW anion but also enhance the compatibility of the catalyst in the reaction medium, thereby improving the catalytic performance.</jats:p>
通讯机构：
[Haiying Cui; Lin Lin] S;[Xiangzhou Li] C;School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China<&wdkj&>School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China<&wdkj&>State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410007, China
期刊：
Progress in Organic Coatings,2019年127:408-418 ISSN：0300-9440
通讯作者：
Li, Xiangzhou
作者机构：
[Liu, Yanxin; Zhou, Jun; Li, Xiangzhou; Wu, Zhiping; Qian, Wei] Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Qian, Wei] Guangdong Polytech Environm Protect Engn, Nanhai 528216, Foshan, Peoples R China.;[Li, Xiangzhou] State Key Lab Ecol Appl Technol Forest Area South, Changsha 410004, Hunan, Peoples R China.;[Liu, Yanxin] Changsha Univ Sci & Technol, Sch Chem & Bioengn, Changsha 410114, Hunan, Peoples R China.
通讯机构：
[Li, Xiangzhou] C;Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Tea saponin;CTS-IFRCs;Synergistic effects;Multi-stage gas phase flame-retardant mechanism;Pyrolysis kinetic performance
摘要：
Tea saponin intumescent flame-retardant coatings (TS-IFRCs) is a green and high-efficiency flame-retardant coating based on natural-based tea saponin (TS), and has been confirmed with superior flame retardancy. The present study determined and assessed the effects of TS on the flame-retardant mechanism in composite tea saponin intumescent flame-retardant coatings (CTS-IFRCs) through the analysis of TG-DSC, FT-IR, SEM-EDS and pyrolysis kinetics. It has been demonstrated from the results that TS, synergized with other components in CTS-IFRCs, has direct and significant impacts on the char layer microstructure, flame retardancy and pyrolysis performance, these influences contributed to the double effects of TS as a blowing agent in gas phase flame-retardant mechanism, and as a carbon agent in condensed phase flame-retardant mechanism. High synergistic effects of natural-based TS revealed in this study have provided effective theoretical basis for emerging environmentally friendly bio-based flame-retardant materials and explored a new way for the utilization of TS.
作者机构：
[Zhou, Jun; Hu, Wei; Deng, Jiao; Liu, Xin; Li, Xiangzhou; Wang, Liqun] Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Li, Xiangzhou] Natl Engn Lab Appl Technol Forestry & Ecol South, Changsha 410004, Hunan, Peoples R China.;[Zhou, Jun; Li, Xiangzhou] Cent South Univ Forestry & Technol, Insititute Nat Prod Res & Dev, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li, Xiangzhou] C;[Li, Xiangzhou] N;Cent South Univ Forestry & Technol, Coll Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;Natl Engn Lab Appl Technol Forestry & Ecol South, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Insititute Nat Prod Res & Dev, Changsha 410004, Hunan, Peoples R China.
摘要：
Litsea cubeba essential oil (LCEO) extracted from the fruit of the Litsea tree is a broad-spectrum bacteriostatic agent that has been used to treat ailments for thousands of years in China. The objective of our study was to assess the inhibitory effect of LCEO on Botrytis cinerea, a fungus that causes the putrification of fruits and vegetables. After being treated with 1.0% LCEO, the electrical conductivity of the fungal cells increased, and the contents of soluble reducing sugars and proteins slowly increased over treatment time. After being treated for 48 h with 1.0% LCEO, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images clearly showed damage to hyphae cells when compared with the normal growth of the control groups. Additional studies showed that the ergosterol content in the cell membrane significantly decreased with an increase in the LCEO concentration, and the electrophoretic bands of the proteins assayed using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis method significantly changed at different LCEO concentrations. LCEO damaged the cell membrane and changed the cell membrane permeability, leading to the changes in some components in the cytoplasm, such as soluble reducing sugars, proteins, and ergosterol. In general, the antimicrobial activity of LCEO is attributable to a unique pathway and involves a series of events both on the surface and within the cytoplasm of the fungal cell.
摘要：
Puerarin is a C-glycoside of daidzein, one of the major bioactive ingredients isolated from the root of Pueraria lobata, which has a wide spectrum of pharmacological effects. Although puerarin is well-known for its effective antioxidant activity, there is seldom a systematic theoretical study on its radical scavenging activity. Herein, the free radical scavenging ability of puerarin was investigated systematically by density functional theory (DFT) calculations. The reaction activity was compared with daidzein as well. Three reaction pathways: hydrogen atom transfer (HAT), single electron transfer followed by proton transfer (SET-PT), and sequential proton loss electron transfer (SPLET) were discussed and compared by thermodynamic parameters such as bond dissociation enthalpy (BDE), ionization potential (IP), proton dissociation enthalpy (PDE), proton affinity (PA), and electron transfer enthalpy (ETE). The reaction kinetics of puerarin with special radicals *OH and *OOH were also studied. The results obtained may be of great significance for better understanding the relationship between the antioxidant properties and structural design of puerarin, as well as other antioxidants.
摘要：
Viniferifuran is an important natural resveratrol dimer with antioxidant, antiviral, anti-inflammatory and other biological activities. In this paper, density functional calculations are carried out to study the antioxidant activity of viniferifuran systematically. The radical scavenging ability of viniferifuran was discussed in detail, thermodynamically and kinetically. All these results suggested that viniferifuran is a stronger antioxidant than resveratrol. These results are of great significance in better understanding the relationship between the antioxidant properties and structural design of antioxidants, and should also be beneficial for promoting the utilization of resveratrol derivatives.
摘要：
The purpose of this study was to improve the solubility of resveratrol (Res) by a self-microemulsifying drug-delivery system (SMEDDS). Through a solubility experiment, the pseudoternary phase diagram and ternary phase diagram were used to optimize the Res SMEDDS formula. The optimum formulation consisted of 5% IPM, 20% PEG400, and 65% Cremophor RH40. The water solubility, stability, in vitro release and antioxidant activity of the Res SMEDDS were characterized. The Res solubility in the SMEDDS was at least 1,000 times compared to that in water. The average droplet size of the microemulsion was 28.00+/-1.67 nm and uniform distribution. The Res SMEDDS should be stored at low temperature and in the dark to avoid light conditions. Res SMEDDS was able to improve the in vitro release rate of Res, and the in vitro release of Res from Res SMEDDS was significantly faster that of Res powder and unaffected by pH value of media. Antioxidant assays showed that antioxidant activities of Res in Res SMEDDS were unaffected compared to Res powder. Cytotoxicity study indicated that Res SMEDDS at the concentration of less than 100 muM was safe. These results demonstrated the potential use of Res SMEDDS for oral administration of Res.
作者机构：
[Zeng Z.; 李湘洲; 张胜] College of Materials Science and Engineering of Central South University of Forestry and Technology, Changsha, 410004, China;State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation), Hunan University of Chinese Medicine, Changsha, 410208, China;[黄丹] College of Materials Science and Engineering of Central South University of Forestry and Technology, Changsha, 410004, China<&wdkj&>State Key Laboratory of Chinese Medicine Powder and Medicine Innovation in Hunan (Incubation), Hunan University of Chinese Medicine, Changsha, 410208, China
通讯机构：
College of Materials Science and Engineering of Central South University of Forestry and Technology, Changsha, China
摘要：
We isolated and purified polysaccharide from the Camellia oleifera Abel. seed cake (CCP) and studied its hypoglycemic activity in vitro. The molecular weight of CCP was 4736 Da and the monosaccharide molar composition ratio was: Xylose:Glucuronic acid:Galactosamine:Mannose = 10.9:4.4:2.6:1.8. The CCP contained conjugated proteins and belonged to acidic polysaccharide, which can promote the consumption of the glucose in the medium by HepG2 cells within the range of 0.125-0.500 mg/mL Relative consumption rate (RCR) of the glucose was the highest at 0.5 mg/ml., exceeded that of the metformin hydrochloride, as well as that of Tea Polysaccharide under the same concentration. A meaningful structure activity relationship (SAR) of hypoglycemia polysaccharides had established. This result suggests that CCP can be used as hypoglycemic medicine or health food. (C) 2018 Elsevier B.V. All rights reserved.
