油茶花粉数量及4 ℃贮藏萌发率特性研究
作者:
邹锋;谭晓风;袁德义;谢鹏;袁军
期刊:
江西农业大学学报 ,2009年31(5):892-895 ISSN:1000-2286
作者机构:
[邹锋; 袁德义; 袁军; 谢鹏; 谭晓风] 中南林业科技大学
关键词:
油茶;花粉数量;萌发率;生活力
摘要:
以长尾红山茶、溆浦大红花、扁果红山茶、石果红山茶、多齿红山茶、长毛红山茶、广宁红山茶、攸县油茶、厚叶红山茶的新鲜花粉为试材,研究花药内花粉数量、花粉离体培养萌发率及4 ℃贮藏下的生活力变化.结果表明:(1)9种油茶单粒花药的花粉平均数量分别为2 168.89、1 711.11、2 648.89、1 191.11、1 555.56、5 128.89、2 560.00、2 595.55、1 751.11粒.(2)在最佳培养基中,9种油茶的萌发率分别为84.9%、99.0%、73.8%、75.3%、81.4%、94.4%、73.8%、72.0%、95.3%.(3)花粉生活力随冷藏时间的延长呈下降的趋势.攸县油茶、广宁红山茶、长尾红山茶、多齿红山茶可以有效保存7 d;石果红山茶、厚叶红山茶、扁果红山茶可以有效保存14 d;长毛红山茶和溆浦大红花可以有效保存21 d.
语种:
中文
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拟南芥悬浮细胞超低温保存试验
作者:
李建安;胡芳名;谭晓风;孙颖
期刊:
中南林业科技大学学报 ,2009年29(6):40-44 ISSN:1673-923X
作者机构:
[胡芳名; 孙颖; 谭晓风; 李建安] 中南林业科技大学
关键词:
生物技术;拟南芥;悬浮细胞;超低温保存
摘要:
试验探索了一般实验室条件下拟南芥悬浮细胞超低温保存具有较高TTC检测活性的简便方法.结果表明:选择继代后2 d的拟南芥悬浮细胞,洗涤1次,细胞沉淀重悬浮于6%蔗糖培养基,于4℃低温下锻炼6 h;浓缩细胞使之有较高的细胞密度(0.25~0.30 g/mL),加入160 g/L甘油作为冰冻保护剂,直接置于-80℃深冷冰箱保存;使用时以37℃快速解冻,离心后除去保护剂,然后用去离子水洗涤后进行TTC活性检测,保存30 d后的细胞相对活性达0.8左右,可以满足实验室一般研究用.
语种:
中文
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中国白梨PbSFBB13-gamma基因的分子克隆与序列特征
作者:
张琳;谭晓风;胡姣;乌云塔娜;袁德义;...
期刊:
林业科学 ,2009年45(11):36-43 ISSN:1001-7488
作者机构:
[张琳; 谭晓风; 胡姣; 乌云塔娜; 袁德义; 龙洪旭] 中南林业科技大学资源与环境学院;[何小勇] 浙江省丽水市科普工作指导站;[李秀根] 中国农业科学研究院郑州果树研究所
关键词:
白梨;配子体自交不亲和性;花粉S基因;S位点F-box基因;进化树
摘要:
以5个已知S基因型的中国白梨品种为试材,设计分别对应于梨SFBB-alpha,SFBB-beta和SFBB-gamma 基因的3对引物对这5个品种进行基因组PCR扩增.结果仅引物组合PSFBG-F/PSFBG-R从‘金花'(S_(13)S_(18)),‘金花四号'((S_(13)S_(18))和‘鹅梨'((S_(13)S_(34))中扩增出一约1 300 bp的条带,经回收、克隆、测序后,鉴定其为SFBB-gamma基因,命名为PbSFBB13-gamma(Pyrus bretschneideri SFBB13-gamma),GenBank登录号为EU081892.逆转录PCR结果表明PbSFBB13-gamma仅在花粉中特异表达;序列分析表明该基因不含内含子,编码区含1 191个核苷酸,编码396个氨基酸,预测分子质量与等电点分别为45.4 ku、4.63.PbSFBB13-gamma表现出典型的花粉SFB/SLF基因的基本结构特征,即1个F-box结构域及4个可变区;在推导氨基酸水平上,其与蔷薇科SFB/SLF基因的相似性为17.8%-97.7%.试验结果充分表明PbSFBB13-gamma应为花粉S基因的候选基因.选取蔷薇科34个SFB/SLF基因的全长氨基酸序列,构建进化树研究基因间的进化关系.结果表明,34个SFB/SLF基因形成2个亚科特异的类群,但不形成种特异的类群,其进化规律与蔷薇科S-RNase基因一致,即花粉SFB/SLF基因的形成也是在亚科形成之后、种形成之前.研究结果有助于从分子水平上揭示中国白梨的自交不亲和性机制.
语种:
中文
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cDNA cloning, sequence analysis and homology modeling of S-20-RNase in Chinese white pear (Pyrus bretschneideri Rehd.)
作者:
Yuan Deyi* ;Zhang Lin;Tan Xiaofeng;Shen Yan
期刊:
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 ,2009年:413-+
通讯作者:
Yuan Deyi
作者机构:
[Tan Xiaofeng; Yuan Deyi; Zhang Lin] Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.;[Shen Yan] Cent South Univ Forestry & Technol, Sch Adult Educ, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Yuan Deyi] C;Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.
关键词:
Chinese white pear;gametophytic self-incompatibility;rapid amplification of cDNA ends;S-20-RNase;three-dimension (3D) structure
摘要:
To elucidate the function of S-allele and the possible molecular mechanism of gametophytic self-incompatibility (GSI) in Chinese white pear (Pyrus bretschneideri Rehd.), full length cDNA encoding S20-RNase was cloned by rapid amplification of cDNA ends (RACE) approach from the cultivar 'Donghuang' (S8S20), and analyzed by bioinformatics method. S20-RNase displayed typical sequence features of pear S-RNases, i.e. five conserved regions and a hypervariable (HV) region. The predicted three-dimension (3D) structure of the S20-RNase was similar to that of S3-RNase of Japanese pear (P. pyrifolia), indicating that the S20-RNase gene behaves as RNase rejecting pollen tube growth with the same S-haplotype as S3-RNase does in GSI response. At the amino acid level, S20-RNase showed 31.5% to 90.8% similarities with other rosaceous S-RNases. Phylogenetic analysis revealed that rosaceous S-RNases occurred before divergence of species, but after divergence of subfamilies Maloideae and Amygdaloideae. ©2009 IEEE.
语种:
英文
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cDNA cloning and bioinformatic analysis of self-incompatible S(34)-allele from Chinese Pears
作者:
Zeng, Yanling* ;Tan, Xiaofeng;Zhang, Dangquan;Hu, Xiaoyi;Chen, Hongpeng;...
期刊:
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 ,2009年:808-811
通讯作者:
Zeng, Yanling
作者机构:
[Zeng, Yanling; Hu, Xiaoyi; Yuan, Deyi; Tan, Xiaofeng; Zhang, Dangquan; Chen, Hongpeng; Zhang, Lin] Cent S Univ Forestry & Technol, Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Zeng, Yanling] C;Cent S Univ Forestry & Technol, Nonwood Forest Prod State Forestry Adm, Changsha 410004, Hunan, Peoples R China.
会议名称:
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)
会议时间:
2009-06-11
会议地点:
北京
会议论文集名称:
The 3rd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2009)(第三届生物信息与生物医学工程国际会议)论文集
关键词:
self-incompatibility;pear;full-length cDNA;S-allele;bioinformatic analysis
摘要:
The full-length cDNA of a new S-RNase (S34-RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The S34-RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of S34-RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning of S34-RNase with other 39 S-RNases of pears revealed the question about unifying naming system. ©2009 IEEE.
语种:
英文
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Cloning and Sequence Characterization of SFBB (S-Locus F-Box Brothers)-Gamma Genes in Chinese Sand Pear (Pyrus pyrifolia Nakai.)
作者:
Tan Xiaofeng* ;Zhang Lin;Long Hongxu;Hu Jiao;He Gongxiu
期刊:
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11 ,2009年:453-456
通讯作者:
Tan Xiaofeng
作者机构:
[Tan Xiaofeng; Long Hongxu; Zhang Lin; Hu Jiao] Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.;[He Gongxiu] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Tan Xiaofeng] C;Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.
会议名称:
iCBBE 2009
会议时间:
2009-01-01
会议地点:
Beijing, China
会议论文集名称:
The 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2009). [v.1]
关键词:
Chinese sand pear;Gametophytic self-incompatibility;S-genotype;S-haplotype;SFBB gene
摘要:
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. It is controlled by a single multi-allelic Slocus which contains two separate genes: one stylar S-RNase gene and one pollen S-gene. In gametophytic self-incompatible Japanese pear (Pyrus pyrifolia Nakai.), SFBB-gamma genes have been identified as good candidates for pollen S-genes. To investigate sequence characters of the possible pollen S-gene in Chinese sand pear, degenerate primers PSFBG-F and PSFBG-R were designed for cloning the SFBB-gamma homologies in eight Chinese sand pear cultivars. By genomic PCR, sequencing and sequence analysis, a total of nine distinct sequences were identified which displayed the basic structural characterization of SFB/SLF genes, i.e. an F-box motif and four variable regions. Of the nine sequences, five equaled to reported Japanese pear SFBB-gamma genes (S1, S2, S4, S5 and S7) and the other four were considered to be new ones which were named SFBB12-gamma, SFBB13-gamma, SFBB15-gamma and SFBB22-gamma. The four new SFBB-gamma genes were expressed specifically in pollen grains. At the deduced amino acid level, they shared 20.4% to 97.7% similarities with other SFB/SLFs of rosaceous plants. The four SFBB-gamma genes cloned in this work will be useful for studying the interaction between SFBs and S-RNases, and clarifying the mechanism of GSI at the molecular level in Chinese pear. ©2009 IEEE.
语种:
英文
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油桐种子EST文库构建及主要表达基因的分析
作者:
谭晓风;谢禄山;张琳
作者机构:
[谭晓风; 谢禄山; 张琳] 中南林业科技大学经济林育种与栽培国家林业局重点实验室;[谭晓风; 谢禄山; 张琳] 中南林业科技大学林学院
会议名称:
第二届中国林业学术大会——S9 木本粮油产业化
会议时间:
2009-11-07
会议地点:
中国广西南宁
关键词:
油桐;桐酸;cDNA文库;EST文库;表达基因
摘要:
油桐是中国的高品质工业油料树种,种子含油率52%-64%,桐油是一种优良的干性植物油,其主要成分是一种共轭三烯酸即桐酸(9,11,13-十八碳三烯酸),桐酸遇空气氧化成膜。为了研究与桐油合成相关的基因表达和分离基因,我们以发育中的对年桐种子为材料构建了cDNA文库,并随机挑取3107个克隆进行5’-端测序构建表达序列标签EST文库,并进一步应用生物信息学方法在NCBI数据库中与其他植物核酸非冗余数
语种:
中文
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太行山山前坡地不同土地利用方式下土壤水分的时空变异特征
作者:
王齐瑞;谭晓风;高峻
期刊:
水土保持学报 ,2008年22(4):100-103,110 ISSN:1009-2242
作者机构:
[王齐瑞; 谭晓风] 中南林业科技大学;[高峻] 中国林业科学研究院林业研究所
关键词:
土地利用方式;土壤水分;时空变异
摘要:
以济源试区坡耕地、苹果园、果农复合系统、退耕还林地、刺槐林地5个邻接农林模式为研究对象,用时域反射仪(TDR)测定坡面不同农林模式雨季前、雨季中和雨季后表层土壤(0-30 cm)含水量,分析表层土壤水分分布特征及其时空变异特征。结果表明:由于受不同坡位、不同植被类型的影响,土壤表层含水量从坡顶到坡底逐渐增加;雨季前后土壤表层含水量的半方差理论函数均呈球状模型,块金值变化并不明显,其值分别为0.25和0.30,独立间距分别为99.7 m和87.6 m,土壤水分具有强烈的空间自相关性,存在很好的分形特征,分形维数分别为1.71和1.74,变异均主要是由空间自相关部分引起。雨季中土壤表层含水量的半方差函数呈直线模型,土壤水分空间变异表现出随距离增加而增加,分形维数为1.40。
语种:
中文
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应用ISSR分析油茶无性系的遗传多样性
作者:
王保明;陈永忠;谭晓风;彭邵锋;石明旺
期刊:
东北林业大学学报 ,2008年36(6):19-23,36 ISSN:1000-5382
作者机构:
经济林育种与栽培国家林业局重点实验室(中南林业科技大学),长沙,410004;[陈永忠; 彭邵锋] 湖南省林业科学院;[王保明; 谭晓风; 石明旺] 中南林业科技大学
关键词:
油茶;优良无性系;遗传多样性
摘要:
以23个油茶无性系叶片的DNA作为实验材料,从60个随机引物中筛选出10个最优引物,并扩增出102条谱带,其中多态性谱带70条,多态性比率为68.6%,谱带大小为200~2000 bp.Shannon遗传信息指数为0.4793,平均观察等位基因数和有效等位基因数分别为2.000和1.519;遗传距离为0.1542~0.6931,油茶无性系之间具有较高多态性.运用平均聚类法分析23个油茶无性系的亲缘关系及多样性,在遗传距离为0.392时,23个油茶无性系聚为4类:湘林156、湘林210和湘林4号等18个油茶无性系为第1类;岑软2号和湘林97号为第2类;湘林34号和湘林11为第3类;湘林81号单独为1类.其中,湘林4号与湘林29的亲缘关系最近,湘林34与湘林78的亲缘关系最远.在此基础上,分析了油茶ISSR聚类结果与油茶农艺学性状的关系,从而为油茶优良无性系甄别、亲缘关系分析以及种质资源利用,在分子水平上积累了有效证据.
语种:
中文
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The cDNA cloning and characteristic of stearoyl-ACP desaturase gene of Camellia oleifera
作者:
Zhang, Dang-Quan* ;Tan, Xiao-Feng;Xie, Lu-Shan;Chen, Hong-Peng;Qiu, Jian;...
期刊:
Acta Horticulturae ,2008年769:55-61 ISSN:0567-7572
通讯作者:
Zhang, Dang-Quan
作者机构:
[Zhang, Dang-Quan; Tan, Xiao-Feng; Hu, Fang-Ming; Chen, Hong-Peng; Qiu, Jian; Xie, Lu-Shan] Cent S Univ Forestry & Technol, Key Lab Nonwood Forest Prod Forestry Minist, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Zhang, Dang-Quan] C;Cent S Univ Forestry & Technol, Key Lab Nonwood Forest Prod Forestry Minist, Changsha 410004, Hunan, Peoples R China.
关键词:
Bioinformatic analysis;Camellia oleifera;SAD;Structural characteristic;Unsaturated fatty acids
摘要:
As the most important and special edible oil tree in China, tea-oil tree (Camellia oleifera) has the oil-yielded rate of about 55% from its kernel. The recent researches suggested that the oiliness of tea-oil should be one of the best plant oils, for tea-oil consists of ∼90% unsaturated fatty acids (∼80% oleic acid, ∼10% linoleic acid and linolenic acid). As the enzyme controlling the first-step desaturation during the biosynthesis of plant unsaturated fatty acids, plant SAD (Stearoyl-ACP desaturase) directly regulates the proportion of saturated fatty acids and unsaturated fatty acids by dehydrogenating saturated fatty acids bonded to ACP (Acyl carrier protein) to form oleic acids. However, recent researches on Camellia oleifera were mostly focused on the conventional planting and breeding and characteristic analysis of its products, and lacked those to find out the molecular mechanism of controlling the forming of high content of oleic acids. Therefore, we attempted to clone the full-length cDNA of Camellia oleifera SAD (CoSAD) gene. We successfully constructed a full-length cDNA library using the nearly matured seeds of elite cultivar Xianglin-4 planted widely in China, and then randomly selected hundreds of clones for sequencing from the 5′-end to construct a good representational EST library. Basing on the homological analyses of this EST library, we obtained 3 ESTs of CoSAD, and then obtained the full-length cDNA of CoSAD from the full-length cDNA library by PCR. The result of bioinformatic analyses including three-dimensional homological modeling on the CoSAD cDNA suggested that CoSAD displays high similarity to SADs of different plants, and is more advantageous than other plant SADs to catalyzing saturated fatty acids into oleic acids. This result could explain the higher content of oleic acids than those of other oil plants, presenting theoretical element for the application of CoSAD genes and genetic improvement on other oil plants.
语种:
英文
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杏-紫花苜蓿生草栽培系统根系分布及生长动态
作者:
王齐瑞;樊巍;谭晓风
期刊:
林业科学 ,2008年44(8):141-144 ISSN:1001-7488
作者机构:
[王齐瑞; 谭晓风] 中南林业科技大学;[樊巍] 河南省林业科学研究院
关键词:
杏;紫花苜蓿;生草栽培;根系;空间分布特征;生长动态
摘要:
果园生草栽培是在果树行间或全园种植草本植物作为土壤覆盖的一种生态果园培育模式。二次大战后生草栽培在国外得到迅速发展,目前,欧美及日本生草果园面积占果园总面积的55%~70%以上(姚胜蕊等,1999)。实际上,种植和利用绿肥是我国农业生产的传统经验之一:早在公元6世纪人们就认识到果园种植豆科作物有好处(吴光林,1992).
语种:
中文
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植物亲环素基因的结构、功能及表达调控
作者:
王保明;谭晓风
期刊:
中南林业科技大学学报 ,2008年28(1):168-174 ISSN:1673-923X
作者机构:
[王保明; 谭晓风] 中南林业科技大学
关键词:
生物技术与工程;亲环素;基因;结构;功能;表达;综述
摘要:
亲环素是一类多基因蛋白质家族,广泛分布于脊椎动物、无脊椎动物、其他后生动物、植物、真菌及原核生物等几乎所有生命体中,存在于细胞液和各个细胞器中.亲环紊在结构上高度保守,具有肽脯氨酰顺反异构酶的活性,参与免疫抑制、蛋白质折叠以及细胞信号传导等多种生物学过程.植物亲环素与其他生物亲环素的结构具有一定的相似性,但含有特有的7个氨基酸插入序列KS/MGKPLH,该插入序列的功能及重要性尚不清楚.植物亲环素基因除具有一般亲环素的功能外,还在植物生物学、胁迫应答、抗病原体免疫等方面发挥重要作用.植物亲环素的基因表达和积累受各种生物因素和非生物因素等逆境条件的影响和调节.但是,许多植物亲环素的功能研究仍然停留在推导和假设水平,还需要开展进一步的实验研究加以证明.
语种:
中文
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撒哈拉沙漠蔓延区生态恢复先锋树种选择试验
作者:
朱宁华;谭晓风
期刊:
中南林业科技大学学报 ,2008年28(6):66-70 ISSN:1673-923X
作者机构:
[朱宁华; 谭晓风] 中南林业科技大学,林学院
关键词:
林学;森林培育学;沙漠化地区;先锋树种;试验研究
摘要:
先锋树种选择是沙漠化地区生态恢复工作的关键技术之一.根据试验地区的具体条件,选择5种抗旱树种作为先锋树种,通过对其种苗、造林技术、森林培育措施等环节的试验,对其树高、胸径、冠幅和存活率连续4年监测数据进行了统计分析.对先锋树种林分对林地环境因子的影响进行了试验研究.结果表明:刺伞相思、巨大戟、赤桉、东非车桑于4个试验树种可以作为该地区的先锋树种;在旱季先锋树种林分对林地土壤温度和林地土壤湿度两方面能起到良性作用,能为后续树种创造较优越的生长环境条件.
语种:
中文
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Cloning and sequence analysis of full length cDNA encoding S16-RNase from 'Xuehua' pear (Pyrus bretschneideri Rehd. )
作者:
谭晓风;张琳;袁德义;曾艳玲;姜傲芳
期刊:
浙江大学学报(农业与生命科学版) ,2008年34(2):149-157 ISSN:1008-9209
作者机构:
[谭晓风; 张琳; 袁德义; ZENG Yanling; 姜傲芳] Central South University of Forestry and Technology
关键词:
配子体自交不亲和;雪花梨;快速扩增cDNA末端;S16-RNase基因
摘要:
通过逆转录PCR(RT-PCR)及快速扩增cDNA末端(RACE)技术从中国白梨品种‘雪花’梨中克隆到S16-RNase基因的全长cDNA序列(GenBank接受号为DQ991388).该cDNA克隆总长1101bp,包含1个完整的开放阅读框,编码228个氨基酸.S16-RNase表现出梨S-RNase基因的基本结构特征,即其具有5个保守区(C1,C2,C3,RC4及C5)和1个高变区.在推导氨基酸水平上,S16-RNase与梨其他S-RNase基因的相似性为63%至74%,但与苹果S16-RNase的相似性高达95%.通过多序列比对构建进化树,分析梨S16-RNase与蔷薇科植物其他S-RNase基因的遗传进化关系.结果表明,S16-RNase与苹果亚科S-RNase基因形成一个亚科特异而非种特异的S-RNase基因类群;且不同S-RNase基因间存在属内种间遗传距离远于属间种间遗传距离现象.
语种:
中文
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Determination of S-Genotypes of Seven Cultivars and Identification of a Novel S-RNase Allele in Pyrus pyrifolia
作者:
张琳;谭晓风;周建;何小勇;袁德义;...
期刊:
林业科学 ,2008年44(10):42-48 ISSN:1001-7488
作者机构:
[张琳; 谭晓风; 袁德义] College of Resources and Environment, Central South University of Forestry and Technology;[周建] Botanical Garden Institute,Henan Institute of Science and Technology;[何小勇] Lishui Popular Science Station of Zhejiang Province;[胡姣; 龙洪旭] Co
关键词:
砂梨;配子体自交不亲和性;自交不亲和基因型
摘要:
砂梨是重要的经济树种,表现出典型的配子体自交不亲和性,在生产和育种上需鉴定品种的S基因犁以确定品种间的亲和性.选取7个砂梨品种为试验材料,使用梨S-RNase(S基因)通用引物进行基因组PCR扩增,产物通过1.8%的琼脂糖凝胶电泳分析.结果表明:‘楚比香'等4个品种中产生了预期的2条电泳条带,而其他3个品种都只产生1条带产物,通过6%的聚丙烯酰胺电泳对该3个品种的PCR产物进一步分析,结果产物被成功分离.将7个品种中分离到的14个条带分别回收、克隆、测序及序列分析,从中鉴别出10个具有梨S-RNase基因序列特征的S基因,其中‘政和大雪梨'中494 bp的基因片段被鉴定为新的S基因,暂命名为S43-RNase(GenBank接受号EF566873).RT-PCR试验证明S43-RNase仅在化柱中特异表达,符合S-RNase的表达特征.通过比对S43-RNase的基因组序列和cDNA序列,确定其内含子大小为294 bp.在推导氨基酸水平上,S43-RNase与苹果亚科其他S-RNase表现出65%~92%的相似性.
语种:
中文
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Study on the rooting of gerbera jamesonii in vitro in media without agar
作者:
Wang, X. -H.* ;Tan, X. -F.;Hu, F. -M.
期刊:
Acta Horticulturae ,2008年771(771):239-242 ISSN:0567-7572
通讯作者:
Wang, X. -H.
作者机构:
[Tan, X. -F.; Hu, F. -M.; Wang, X. -H.] Ctr S Univ Forest & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha, Hunan, Peoples R China.
通讯机构:
[Wang, X. -H.] C;Ctr S Univ Forest & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha, Hunan, Peoples R China.
关键词:
Gerbera jamesonii;Non-organic medium;Seedling;Survival rate of transplant;Tissue culture
摘要:
There are two tough problems in tissue culture of plants. One is high cost, another is low survival rate of transplant. In order to reduce the cost of tissue culture with agar, and to increase survival rate of transplant, another kind of support in culture medium instead of agar is needed, which is cheap and can be washed down easily. We respectively put the buds of Gerbera jamesonii in nutrient solution (MS+BA 15 mg.L-1 +IBA 0.2 mg.L-1) with three kinds of medium in vitro to induce to root, which are grit, sponge, and agar. And then rooted plantlets were transplanted from culture bottle to flower bed. At the same time, rooting rate and survival rate of transplant were counted. Results were showed: the rooting rate was highest in nutrient solution with grit (which were respectively 96.55, 89.47, 94.11% with grit, sponge, and agar), and the panorama of growth was also best; Furthermore, its survival rate of transplant was highest (which were respectively 97.63, 96.25, 78.18% with grit, sponge, and agar). Moreover, there were some details affecting the results such as the volume of liquid, and the way to treat support materials. The success in the rooting culture of Gerbera jamesonii without agar showed that grit and sponge could be used instead of agar. If the technique can be widely used in tissue culture of other plants, it will be great meaningful in the field of plants.
语种:
英文
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油茶花粉特性及其不同贮藏条件下生活力的研究
作者:
袁德义;谭晓风;胡青素;邹峰
期刊:
浙江林业科技 ,2008年28(5):66-69 ISSN:1001-3776
作者机构:
中南林业科技大学,国家林业局经济林育种与栽培重点实验室,湖南,长沙,410004
关键词:
攸县油茶;黄蛋红花茶;花粉萌发率;生活力;培养基
摘要:
以攸县油茶和黄蛋红花茶的花粉为试验材料,研究了其花药内花粉数量、花粉离体培养萌发率及不同贮藏条件下的花粉活力。结果表明:攸县油茶和黄蛋红花茶的单花药花粉平均数量分别约为5 400粒与6 700粒;二者花粉均在5%蔗糖+0.01%硼酸+1%琼脂的培养基上萌发率最高,萌发率分别为70.9%与73.1%;在4℃冰箱、-20℃冰冻、室温和25℃干燥状态下贮藏1周,攸县油茶和黄蛋红花茶的萌发率分别为35%、2.1%、21.0%、27.5%和36.7%、13.3%、26.7%、21.5%。
语种:
中文
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桉树ISSR-PCR反应体系的建立及优化
作者:
曾艳玲;谢鹏;谢耀坚;谭晓风
期刊:
中南林业科技大学学报 ,2008年28(1):44-48 ISSN:1673-923X
作者机构:
[谢耀坚; 曾艳玲; 谭晓风; 谢鹏] 中南林业科技大学
关键词:
分子生物学;桉树;叶片基因组DNA;优化
摘要:
模板DNA、引物、dNTPs、Mg~(2+)的浓度,Taq DNA聚合酶的用量以及退火温度是影响简单序列重复区间扩增(ISSR-PCR)结果的主要因素.以桉树叶片基因组DNA为试材,系统地测试了这6个因素对桉树ISSR反应结果的影响.结果表明;最优化的反应体系即20μL反应体系中,含20 ng模板DNA、0.4 μmol·L~(-1)随机引物、0.15 mmol·L~(-1) dNTPs,2.0 mmol·L~(-1)Mg~(2+)、1.25 U Taq DNA聚合酶.最佳退火温度为55℃;PCR反应程序为94 ℃预变性5 min; 94 C变性45 s,55 ℃退火45 s,72℃延伸1.5 min,35个循环;72℃再延伸7 min.
语种:
中文
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基于基因芯片的梨品种S基因型鉴定的技术方法
作者:
江南;谭晓风
期刊:
中南林业科技大学学报 ,2007年27(1):104-108 ISSN:1673-923X
作者机构:
[谭晓风] 中南林业科技大学;[江南] 湖南工业大学
关键词:
生物科学与技术;基因芯片;梨;自交不亲和性;S基因型;鉴定
摘要:
梨是典型的配子体自交不亲和植物,梨品种自交不亲和基因型(S基因型)确定的研究对于梨的栽培和遗传改良具有重要的意义.综合分析了运用杂交授粉试验、PCR-RFLP技术、DNA序列分析、cDNA克隆等技术在确定梨品种S基因型方面的优点和不足之处,提出了运用基因芯片技术确定梨品种S基因型的新方法,并分析了该技术的优势以及在梨品种S基因型确定方面的应用前景
语种:
中文
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尾叶桉遗传资源引进及其生物学入侵风险评估
作者:
谢耀坚;谭晓风
期刊:
中南林业科技大学学报 ,2007年27(6):169-173 ISSN:1673-923X
作者机构:
[谢耀坚; 谭晓风] 中南林业科技大学
关键词:
林木遗传育种;尾叶桉;引种;生物学入侵;风险评估
摘要:
尾叶桉为高大乔木,其生长快、干形通直、材性好,原始分布于印度尼西亚.我国自1976年开始引种尾叶桉,分别在广东、广西、海南等地建立了种源/家系试验林,保存了大量的遗传资源并营造了大面积的人工林,现已成为我国推广栽培面积最大的桉树树种之一.桉属树种全部为外来引进树种,我国没有自然分布,不能与我国本土树种发生杂交,不存在与我国本地树种发生基因交流或杂种化的风险.桉树在我国能正常开花结实,但种子特别小,很难自然发芽成活,不能自然更新和扩张,不会出现自发入侵本地生态系统、占据其它树种栖息地、造成生物入侵危害的现象.桉树与其它植物的水肥竞争或化感作用导致其林下植被贫乏还存在争论,但我国华南地区年降雨量多,林下植被一般都很丰富,也有多种动物栖息.大量引种栽培试验也证明,桉树在我国华南一带种植对其它植物的化感作用不明显,桉树人工林的发展完全可以人为控制.
语种:
中文
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