期刊：
FRONTIERS IN PLANT SCIENCE,2024年15:1396635 ISSN：1664-462X
通讯作者：
Zhou, Junqin;Tan, XF;Zhou, JQ
作者机构：
[Lu, Mengqi; Xiao, Zhun; Yang, Liying; Zeng, Yanling; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zhou, JQ] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Peoples R China.;[Lu, Mengqi; Xiao, Zhun; Yang, Liying; Zeng, Yanling; Zhou, Junqin; Tan, Xiaofeng; Gu, Yiyang; Zhou, JQ] Cent South Univ Forestry & Technol, Acad Camellia Oil Tree, Changsha, Peoples R China.;[Yang, Liying] Hunan Acad Agr Sci, Hunan Hort Res Inst, Changsha, Peoples R China.;[Zhou, Junqin; Zhou, JQ] Cent South Univ Forestry & Technol, Coll Landscape Architecture, Changsha, Peoples R China.;[Xiao, Zhun] Changsha Social Work Coll, Sch Foreign Languages, Changsha, Peoples R China.
通讯机构：
[Tan, XF ; Zhou, JQ; Zhou, JQ ] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Peoples R China.;Cent South Univ Forestry & Technol, Acad Camellia Oil Tree, Changsha, Peoples R China.;Cent South Univ Forestry & Technol, Coll Landscape Architecture, Changsha, Peoples R China.
关键词：
Camellia;Camellia oleifera;gene cluster;mitochondrial genome;nad5-nads tandem genes
摘要：
Camellia oleifera is a kind of high-quality oil supply species. Its seeds contain rich unsaturated fatty acids and antioxidant active ingredients, which is a kind of high-quality edible oil. In this study, we used bioinformatics methods to decipher a hexaploid Camellia oil tree's mitochondrial (mt) genome based on second-generation sequencing data. A 709,596 bp circular map of C. oleifera mt genome was found for the first time. And 74 genes were annotated in the whole genome. Mt genomes of C. oleifera and three Theaceae species had regions with high similarity, including gene composition and gene sequence. At the same time, five conserved gene pairs were found in 20 species. In all of the mt genomes, most of nad genes existed in tandem pairs. In addition, the species classification result, which, according to the gene differences in tandem with nad5 genes, was consistent with the phylogenetic tree. These initial results provide a valuable basis for the further researches of Camellia oleifera and a reference for the systematic evolution of plant mt genomes.
通讯机构：
[Xu, D ] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Byproducts Further Proc, Changsha 410004, Peoples R China.
摘要：
Cytoarchitectural staining is of great importance in disease diagnosis and cell biology research. This study developed user-friendly multifunctional red-emissive carbon dots (R-CDs) for rapid cell nucleus staining via targeting nuclear proteins. R-CDs, simply prepared by electrochemical treatment of 1,2,4-benzenetriamine, exhibit strong emission at 635 nm when excited at 507 nm. The R-CDs can rapidly stain the nucleus of human SH-SY5Y, HepG2, and HUH-7 cells with a high signal-to-noise ratio owing to fluorescence enhancement after entering the nucleus. Compared to conventional cytosolic dyes such as Hoechst and DAPI, R-CDs are cheaper, more highly dispersed in water, and more stable (requiring no stringent storage conditions). The R-CDs show stable optical properties with insignificant photobleaching over 7 days and salt resistance up to 2 M of NaCl. More importantly, R-CDs, possessing a positive charge, allow rapid staining of live cells (3 min) and dead cells (10 s) in saline. According to kinetic variation, R-CDs can distinguish live cells from dead cells. Staining exhibits high efficiency in onion epidermal cells, Aspergillus niger, Caenorhabditis elegans, and human spermatozoa. The mechanism for efficient staining is based on their fast accumulation in the nucleus due to their small size and positive charge and strong interaction with nuclear proteins at amino acid residues of histidine and arginine, resulting in fluorescence enhancement by dozens of times. The developed R-CDs do not bind to DNA and would not cause genetic damage and will find various safe applications in biological and medical fields.
摘要：
In this study, we developed a method for synthesizing silver nanoparticles (AgNPs) using aqueous assembly assay and anchoring them with camellia seed cake polyphenols (CPs). Zeta potential analysis demonstrated a higher binding affinity of CP-AgNPs to Penicillium digitatum, which could be attributed to the interaction between the hydroxyl group of CP-AgNPs and the polysaccharide present on the fungal cell wall. Compared to AgNPs, the CP-AgNPs outperformed conventional AgNPs in antifungal activity against P. digitatum. The mechanism underlying this antimicrobial effect involves raising the level of oxidative stress by regulating the expression levels of oxidative stress response genes. In addition, CP-AgNPs/CS showed highly effective in reducing citrus decay levels during chamber storage. In conclusion, the use of CPs as anchoring agent in the in-situ aqueous assembly of CP-AgNPs improved their biocompatibility and antimicrobial properties. CP-AgNPs hold great promise for applications in fruit preservation, especially citrus preservation.
通讯机构：
[Junqin Zhou; Xiaofeng Tan] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China<&wdkj&>Academy of Camellia Oil Tree, Central South University of Forestry and Technology, Changsha, Hunan, China
关键词：
ABA;Camellia oleifera;FLA;Pollen tube growth
摘要：
Fasciclin-like arabinogalactan proteins (FLAs) are a class of highly glycosylated glycoproteins that perform crucial functions in plant growth and development. This study was carried out to further explore their roles in pollen tube growth. The results showed that seven members (CoFLA1/2/3/4/7/8/17) of the CoFLAs family were identified by sequence characteristics, and they all possessed the fasciclin 1 (FAS1) domain and H1 and H2 conserved domains. They were all located on the plasma membranes of tobacco epidermal cells, and the GPI-anchor sequences of CoFLA1/2/3/4 determined the membrane localization. In flower tissues, CoFLA2 and CoFLA8 were not expressed in the pollen tube but were expressed in the unpollinated style and ovary; the others were all expressed in the pollen tube. In the pollination-compatible style and ovary, they exhibited different expression patterns. Furthermore, all CoFLAs promoted pollen germination in vitro, while only CoFLA7 significantly promoted pollen tube elongation, and the expression of CoFLA1/3/4/7/17 in pollen tubes was regulated by CoFLA proteins. The ABA and ABA synthetic inhibitor (sodium tungstate, ST) both inhibited pollen tube elongation; however, only ST downregulated the expression of CoFLA1/7/17 and upregulated the expression of CoFLA4. Taken together, these results demonstrate that CoFLAs may be significant in pollen tube growth in C. oleifera and that some CoFLAs may participate in the regulation of ABA signaling.
通讯机构：
[Junqin Zhou; Xiaofeng Tan] K;Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, 410001 China
摘要：
Salicylic acid (SA) is a plant hormone involved in a series of growth, development, and stress responses in plants. Nonexpressor of pathogenesis-related genes 1 (NPR1) is the core regulatory gene in the process of SA-mediated systemic acquired resistance (SAR). Whether NPR1 is involved in pollen tube growth mediated by SA and its derivative MeSA (methyl salicylate) remains to be explored. Here, we found that the contents of endogenous SA and MeSA in self- or cross-pollinated pistils changed significantly, and exogenous SA and MeSA significantly promoted pollen germination and pollen tube elongation of Camellia oleifera at lower concentrations. CoNPR1, CoNPR3.1, CoNPR3.2, and CoNPR5 were identified, and they were all located in the nucleus. A high level of consistency was observed across the phylogenetic relationships, gene structures, and functional domains, indicating a clear division of function, as observed in other species. The expression levels of CoNPR1, CoNPR3.1, CoNPR3.2, and CoNPR5 in self- and cross-pollinated pistils had certain regularity. Furthermore, they exhibited tissue-specific expression pattern. CoNPR1 and CoNPR3.1 were expressed in pollen tubes, whose expression was regulated by SA or MeSA, and their expression patterns were basically consistent with the trend of pollen germination. These results indicate that SA and MeSA are involved in the pollen tube growth of C. oleifera, and CoNPRs may play an important role therein.
摘要：
【目的】探讨NaCl对油茶花粉生活力、花粉萌发和花粉管生长的影响,以及Ca~(2+)是否有缓解作用,旨在探索在盐渍化地区引种油茶的可能性,为油茶的引种和栽培提供参考资料。【方法】以油茶‘华硕'花粉为试验材料,采用MTT染色法和花粉液体培养法,研究NaCl胁迫以及在NaCl胁迫环境中添加Ca~(2+)后对油茶花粉生活力、萌发率和花粉管生长的影响。【结果】1)NaCl对油茶花粉生活力、花粉萌发和花粉管伸长均有抑制作用,且抑制效果随NaCl浓度的提高而增强,而高浓度NaCl可完全抑制花粉萌发。20 mM NaCl的抑制效果显著(P < 0.05),此时花粉生活力和花粉萌发率分别为36.22%和42.84%,分别是CK的49.97%和57.33%,花粉管长度为23.75 μm,是CK的73.54%。在NaCl胁迫环境中,部分花粉管的顶端有膨大现象。2)在20 mM NaCl的胁迫下,外源Ca~(2+)对花粉生活力有抑制作用,但能部分缓解NaCl对油茶花粉萌发和花粉管伸长的抑制作用。2 mM Ca~(2+)对花粉萌发率的缓解效果最佳,此时花粉萌发率显著提高,为60.5%,而Ca~(2+)浓度超过2 mM时,花粉萌发率显著下降,维持在29%左右,出现抑制作用。花粉管长度随着Ca~(2+)浓度的增加而缓慢增长,25 mM Ca~(2+)对花粉管伸长的促进作用最明显,此时花粉管最长,为35.05 μm。当Ca~(2+)浓度超过3 mM时,在刚萌发出来的花粉管或者较短的花粉管中顶端破裂现象严重。【结论】在花粉的离体培养过程中,NaCl会对油茶花粉生活力、花粉萌发和花粉管伸长造成胁迫,所以,在油茶的栽培和管理过程中,应避免将油茶处在盐胁迫环境中。 Ca~(2+)参与了油茶花粉适应盐环境的过程,可为提高油茶耐盐性的研究奠定理论基础。
摘要：
Camellia oleifera is an economically important edible oil tree that is cultivated over an area of about 4.3 million hectares in southern China. To elucidate the molecular and evolutionary characteristics of C. oleifera chloroplasts, we sequenced and characterized its genome using Illumina high-throughput sequencing (HiSeq) combined with third-generation PacBio sequencing technology. The chloroplast genomes ranged from 156,965 to 156,975 bp, containing 133 genes including 8 rRNAs, 37 tRNAs and 88 protein-coding genes, and had a typical quadripartite structure with conserved genome arrangement. Approximately 50 simple sequence repeats (SSRs) and 37 repeat sequences were identified in the C. oleifera chloroplast genome. A comparison with seven other Camellia complete chloroplast genomes detected only slight differences in the coding regions of some genes, including psbN and ycf1; highly divergent regions were observed in the intergenic spacers (IGS) of the 10 Camellia chloroplast genomes. Phylogenetic analysis suggested a sister relationship among three C. oleifera cultivars ('Huashuo', 'Huajin' and 'Huaxin') and C. oleifera, whereas C. oleifera from Hainan was identified as sister to C. azalea. Based on our evaluation of the chloroplast genomic resources of three C. oleifera cultivars, we assembled the complete chloroplast genomes; the development of abundant C. oleifera genetic resources will contribute to further studies of genetic engineering, phylogeny, and evolution among family Theaceae species. (C) 2020 Friends Science Publishers
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
通讯机构：
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
摘要：
Tung tree (Vernicia fordii), an economically important woody oil plant, is a monoecious and diclinous species with male and female flowers on the same inflorescence. The extremely low proportion of female flowers leads to low fruit yield in tung orchards. The female flower normally develops along with stamen abortion; otherwise sterile ovules will be produced. However, little knowledge is known about the molecular basis of the female flower development in tung tree. In this study, integrated analyses of morphological and cytological observations, endogenous phytohormone assay and RNA-seq were conducted to understand the molecular mechanism of the female flower development in tung tree. Cytological observation suggested that the abortion of stamens in female flowers (SFFs) belongs to the type of programmed cell death (PCD), which was caused by tapetum degeneration at microspore mother cell stage. A total of 1,366 differentially expressed genes (DEGs) were identified in female flowers by RNA-seq analysis, of which 279 (20.42%) DEGs were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction. Stage-specific transcript identification detected dynamically expressed genes of important transcription regulators in female flowers that may be involved in PCD and floral organ development. Gene expression patterns revealed that 17 anther and pollen development genes and 37 PCD-related genes might be involved in the abortion of SFF. Further analyses of phytohormone levels and co-expression networks suggested that salicylic acid (SA) accumulation could trigger PCD and inhibit the development of SFF in tung tree. This study provides new insights into the role of SA in regulating the abortion of SFF to develop normal female flowers.
通讯机构：
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
