植物ACCase 基因的结构功能及表达调控研究
作者：
王保明;颜士华;谭晓风
期刊：
安徽农学通报 ,2021年27(01):17-24 ISSN：1007-7731
作者机构：
湖南应用技术学院农林科技学院;[颜士华] 临沂科技职业学院现代农业学院;[谭晓风] 中南林业科技大学经济林培育与利用湖南省高校“2011”协同创新中心;[王保明] 湖南应用技术学院农林科技学院<&wdkj&>临沂科技职业学院现代农业学院<&wdkj&>中南林业科技大学经济林培育与利用湖南省高校“2011”协同创新中心
关键词：
植物;基因;结构功能;表达调控
摘要：
乙酰辅酶A羧化酶（Acetyl-CoA carboxylase,ACCase）是催化脂肪酸合成的关键和限速步骤。该文介绍了ACCase的分类及结构特征,阐述了其在脂肪合成代谢中的作用和在除草剂中的应用,分析了它的表达调控及反馈机理,揭示了ACCase基因的克隆及表达鉴定,并展望了ACCase在植物育种中的应用前景。
语种：
中文
展开
In-depth understanding of Camellia oleifera self-incompatibility by comparative transcriptome, proteome and metabolome
作者：
Zhou, Junqin;Lu, Mengqi;Yu, Shushu;Liu, Yiyao;Yang, Jin;...
期刊：
International Journal of Molecular Sciences ,2020年21(5):1600 ISSN：1661-6596
通讯作者：
Tan, Xiaofeng
作者机构：
[Lu, Mengqi; Yang, Jin; Liu, Yiyao; Zhou, Junqin; Tan, Xiaofeng; Yu, Shushu] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410001, Peoples R China.
通讯机构：
[Tan, Xiaofeng] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410001, Peoples R China.
关键词：
ABC transporter;mitogen activated protein kinase;phytohormone;ubiquitin;mitogen activated protein kinase;phytohormone;plant protein;proteome;transcriptome;Article;Camellia oleifera;comparative metabolomics;comparative proteomics;comparative transcriptomics;controlled study;cross pollination;cytology;gene expression;genetic incompatibility;MAPK gene;MAPK signaling;metabolomics;nonhuman;PCD gene;pistil;plant gene;plant genetics;protein degradation;protein expression;self-incompatibility (plant);signal transduction;transcriptomics;trend study;apoptosis;Camellia;China;flower;gene expression profiling;gene expression regulation;genetics;metabolism;metabolome;physiology;pollen tube;pollination;proteomics;ubiquitination;Apoptosis;Camellia;China;Flowers;Gene Expression Profiling;Gene Expression Regulation, Plant;Metabolic Networks and Pathways;Metabolome;Mitogen-Activated Protein Kinases;Plant Growth Regulators;Plant Proteins;Pollen Tube;Pollination;Proteome;Proteomics;Transcriptome;Ubiquitination
摘要：
Oil-tea tree (Camellia oleifera) is the most important edible oil tree species in China with late-acting self-incompatibility (LSI) properties. The mechanism of LSI is uncertain, which seriously hinders the research on its genetic characteristics, construction of genetic map, selection of cross breeding parents and cultivar arrangement. To gain insights into the LSI mechanism, we performed cytological, transcriptomic, proteomic and metabolomic studies on self-and cross-pollinated pistils. The studies identified 166,591 transcripts, 6851 proteins and 6455 metabolites. Transcriptomic analysis revealed 1197 differentially expressed transcripts between self-and cross-pollinated pistils and 47 programmed cell death (PCD)-control transcripts. Trend analysis by Pearson correlation categorized nine trend graphs linked to 226 differentially expressed proteins and 38 differentially expressed metabolites. Functional enrichment analysis revealed that the LSI was closely associated with PCD-related genes, mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, ATP-binding cassette (ABC) transporters and ubiquitin-mediated proteolysis. These particular trends in transcripts, proteins and metabolites suggested the involvement of PCD in LSI. The results provide a solid genetic foundation for elucidating the regulatory network of PCD-mediated self-incompatibility in C. oleifera. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
展开
Complete Chloroplast Genome Sequences and Phylogenetic Analysis of Three Camellia oleifera Cultivars
作者：
Wu, Lingli;Jian, Jian'an;Gu, YiYang;Zhang, Fanhang;Li, Ze* ;...
期刊：
International Journal of Agriculture and Biology ,2020年24(4):743-750 ISSN：1560-8530
通讯作者：
Li, Ze;Tan, Xiaofeng
作者机构：
[Li, Ze; Gu, YiYang; Zhang, Fanhang; Wu, Lingli; Jian, Jian'an; Li, Z; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;[Li, Ze; Gu, YiYang; Zhang, Fanhang; Wu, Lingli; Jian, Jian'an; Li, Z; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Peoples R China.;[Li, Ze; Gu, YiYang; Zhang, Fanhang; Wu, Lingli; Jian, Jian'an; Li, Z; Tan, Xiaofeng] Cent South Univ Forestry & Technol, Engn Technol Res Ctr Southern Hilly & Mt Ecol Non, Changsha 410004, Peoples R China.
通讯机构：
[Li, Z; Tan, XF] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Peoples R China.;Cent South Univ Forestry & Technol, Engn Technol Res Ctr Southern Hilly & Mt Ecol Non, Changsha 410004, Peoples R China.
关键词：
Camellia oleifera;Chloroplast genome;Phylogenetic relationship;Repeat analysis
摘要：
Camellia oleifera is an economically important edible oil tree that is cultivated over an area of about 4.3 million hectares in southern China. To elucidate the molecular and evolutionary characteristics of C. oleifera chloroplasts, we sequenced and characterized its genome using Illumina high-throughput sequencing (HiSeq) combined with third-generation PacBio sequencing technology. The chloroplast genomes ranged from 156,965 to 156,975 bp, containing 133 genes including 8 rRNAs, 37 tRNAs and 88 protein-coding genes, and had a typical quadripartite structure with conserved genome arrangement. Approximately 50 simple sequence repeats (SSRs) and 37 repeat sequences were identified in the C. oleifera chloroplast genome. A comparison with seven other Camellia complete chloroplast genomes detected only slight differences in the coding regions of some genes, including psbN and ycf1; highly divergent regions were observed in the intergenic spacers (IGS) of the 10 Camellia chloroplast genomes. Phylogenetic analysis suggested a sister relationship among three C. oleifera cultivars ('Huashuo', 'Huajin' and 'Huaxin') and C. oleifera, whereas C. oleifera from Hainan was identified as sister to C. azalea. Based on our evaluation of the chloroplast genomic resources of three C. oleifera cultivars, we assembled the complete chloroplast genomes; the development of abundant C. oleifera genetic resources will contribute to further studies of genetic engineering, phylogeny, and evolution among family Theaceae species. (C) 2020 Friends Science Publishers
语种：
英文
展开
油茶自交、异交雌蕊脯氨酸含量及相关基因克隆表达分析
作者：
周俊琴;渠心静;卢梦琪;余姝姝;刘懿瑶;...
期刊：
植物生理与分子生物学学报 ,2020年56(3):520-528 ISSN：1671-3877
通讯作者：
Tan, X.
作者机构：
中南林业科技大学经济林培育与保护教育部重点实验室,长沙410004;[刘懿瑶; 周俊琴; 渠心静; 卢梦琪; 谭晓风; 余妹妹; 杨进] 中南林业科技大学
通讯机构：
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, China
关键词：
油茶;自交不亲和;脯氨酸;雌蕊;杂交
摘要：
为研究脯氨酸在油茶（Camellia oleifera）授粉受精过程中的生理作用及相关基因的表达状况,本研究以油茶‘华鑫’为材料,通过反转录PCR （RT-PCR）方法分别克隆出油茶脯氨酸合成途径相关的2个关键基因,即Δ1-吡咯啉-5-羧酸合成酶（P5CS）基因和Δ1-吡咯啉-5-羧酸还原酶（P5CR）基因,以及脯氨酸分解途径相关的2个关键基因,即脯氨酸脱氢酶（ProDH）基因和Δ1-吡咯啉-5-羧酸脱氢酶（P5CDH）基因。克隆的4个基因分别被命名为Co P5CS、Co P5CR、Co Pro DH和Co P5CDH,基因编码区长度分别为2 154、834、1 656和1 473 bp,分别编码538、277、511和490个氨基酸。进化树分析表明, 4个蛋白彼此间的同源性较低,而与茶树（C. sinensis）对应蛋白亲缘关系最近。实时荧光定量PCR结果表明:CoP5CS和CoP5CR在自异交2～96 h期间表达趋势基本一致,在授粉后36h达到最高值,自交授粉表达量明显高于异交授粉; CoP5CDH和CoProDH在授粉后0～36 h期间呈现明显下降的趋势,在36～60 h期间表达量基本没有变化,授粉后60 h有明显上升的趋势。自异交雌蕊游离脯氨酸的含量都在授粉后36 h达到最高值,自交雌蕊脯氨酸含量是异交的1.56倍,表明脯氨酸可能参与了油茶自交花粉管死亡的过程。该研究结果不仅为油茶自交不亲和机制提供新的参考材料,还拟为提高油茶坐果率提供理论支撑。
语种：
中文
展开
油茶ABI5基因的克隆及其表达分析
作者：
杨进;周俊琴;卢梦琪;余姝姝;刘懿瑶;...
期刊：
植物生理与分子生物学学报 ,2020年56(7):1583-1592 ISSN：1671-3877
通讯作者：
Tan, X.
作者机构：
[刘懿瑶; 周俊琴; 余姝姝; 卢梦琪; 谭晓风; 杨进] 中南林业科技大学
通讯机构：
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
关键词：
油茶;自交不亲和;ABI5基因;原核表达;亚细胞定位;表达模式
摘要：
以油茶（Camellia oleifera）主栽品种‘华硕’为试验材料,通过RT-RCR的方法克隆出油茶脱落酸不敏感蛋白5 （abscisic acid-insensitive 5, ABI5）全长cDNA序列,命名为CoABI5,利用不同的在线网站对CoABI5基因进行生物信息学分析,通过原核表达、亚细胞定位和实时荧光定量PCR分别对该基因的表达情况、表达部位和表达模式进行研究分析。结果表明:该序列CDS长度为795 bp,编码264个氨基酸,其中与茶树（Camellia sinensis）的ABI5相似性最高,达到97.73%。原核表达实验发现融合蛋白pCold-CoABI5-TF在37℃诱导4 h后,在85 kDa处出现一条蛋白条带,与理论融合蛋白的分子量一致。亚细胞定位显示该基因定位于细胞核。实时荧光定量PCR结果表明, CoABI5基因在油茶花粉中的表达量最高,自交24 h花柱中的表达量大于‘华硕’ב华鑫’异交,异交48 h子房中的表达量大于自交。本研究对进一步挖掘该基因在油茶后期自交不亲和过程中发挥的作用提供研究基础,对油茶自交不亲和分子机制解析具有重要的意义。
语种：
中文
展开
New Insights of Salicylic Acid Into Stamen Abortion of Female Flowers in Tung Tree (Vernicia fordii)
作者：
Liu, Meilan;Li, Wenying;Zhao, Guang;Fan, Xiaoming;Long, Hongxu;...
期刊：
Frontiers in Genetics ,2019年10(APR):316 ISSN：1664-8021
通讯作者：
Zhang, Lin
作者机构：
[Li, Wenying; Zhao, Guang; Fan, Yanru; Liu, Meilan; Long, Hongxu; Fan, Xiaoming; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;[Li, Wenying; Zhao, Guang; Fan, Yanru; Liu, Meilan; Long, Hongxu; Fan, Xiaoming; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha, Hunan, Peoples R China.;[Shi, Mingwang] Henan Inst Sci & Technol, Xinxiang, Peoples R China.
通讯机构：
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha, Hunan, Peoples R China.
关键词：
flower development;programmed cell death;salicylic acid;stamen abortion;tung tree;unisexual flowers
摘要：
Tung tree (Vernicia fordii), an economically important woody oil plant, is a monoecious and diclinous species with male and female flowers on the same inflorescence. The extremely low proportion of female flowers leads to low fruit yield in tung orchards. The female flower normally develops along with stamen abortion; otherwise sterile ovules will be produced. However, little knowledge is known about the molecular basis of the female flower development in tung tree. In this study, integrated analyses of morphological and cytological observations, endogenous phytohormone assay and RNA-seq were conducted to understand the molecular mechanism of the female flower development in tung tree. Cytological observation suggested that the abortion of stamens in female flowers (SFFs) belongs to the type of programmed cell death (PCD), which was caused by tapetum degeneration at microspore mother cell stage. A total of 1,366 differentially expressed genes (DEGs) were identified in female flowers by RNA-seq analysis, of which 279 (20.42%) DEGs were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction. Stage-specific transcript identification detected dynamically expressed genes of important transcription regulators in female flowers that may be involved in PCD and floral organ development. Gene expression patterns revealed that 17 anther and pollen development genes and 37 PCD-related genes might be involved in the abortion of SFF. Further analyses of phytohormone levels and co-expression networks suggested that salicylic acid (SA) accumulation could trigger PCD and inhibit the development of SFF in tung tree. This study provides new insights into the role of SA in regulating the abortion of SFF to develop normal female flowers.
语种：
英文
展开
不同规格容器对油桐幼苗生长及光合特性的影响
作者：
张帆航;顾伊阳;李泽;谭晓风;李文
期刊：
中南林业科技大学学报 ,2019年39(10):71-75,122 ISSN：1673-923X
作者机构：
中南林业科技大学, 经济林培育与保护教育部重点实验室, 湖南, 长沙, 410004;中南林业科技大学森林培育实验室, 湖南, 长沙, 410004;[李泽; 张帆航; 顾伊阳; 李文; 谭晓风] 中南林业科技大学
关键词：
油桐;不同规格容器;生长特性;光合特性
摘要：
为了筛选出适宜油桐幼苗生长的育苗容器规格,为油桐盆栽试验的开展提供理论依据,为油桐育苗、造林速度及造林质量的提高提供理论参考依据。选取1年生油桐幼苗作为试验材料,采用盆栽试验的方法,研究不同规格的育苗容器对油桐幼苗生长及光合特性的影响。结果表明,随着容器规格的降低,油桐幼苗的株高、地径、叶绿素a含量(Chl a)、叶绿素b含量(Chl b)、叶绿素总量(Chl a+b)、净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、气孔限制值(L_s)、最大净光合速率(P_(nmax))和光饱和点(LSP)逐步下降,差异显著(P < 0.05),胞间二氧化碳浓度(C_i)、光补偿点(LCP)和暗呼吸速率(R_d)逐渐升高,差异显著(P < 0.05),而叶绿素a/b(Chl a/b)变化不显著(P > 0.05)。研究表明,大规格容器利于油桐幼苗的生长,在油桐栽培试验中,可选择规格Ⅰ及以上规格的容器,但在容器育苗中,选择规格Ⅱ的容器,既能保证苗木质量,还有利于控制育苗成本。
语种：
中文
展开
Boosting C16 fatty acid biosynthesis of Escherichia coli, yeast and tobacco by tung tree (Vernicia fordii Hemsl.) beta-hydroxyacyl-acyl carrier protein dehydratase gene
作者：
Liu, Meilan;Long, Hongxu;Li, Wengying;Shi, Mingwang;Cao, Heping;...
期刊：
Industrial Crops and Products ,2019年127:46-54 ISSN：0926-6690
通讯作者：
Zhang, Lin;Tan, Xiaofeng
作者机构：
[Zhang, Lin; Tan, Xiaofeng; Liu, Meilan; Long, Hongxu; Li, Wengying] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.;[Shi, Mingwang] Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.;[Cao, Heping] ARS, USDA, Southern Reg Res Ctr, New Orleans, LA USA.
通讯机构：
[Zhang, L; Tan, XF] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.
关键词：
Fatty acid synthase;Gene expression pattern;Heterologous expression;Subcellular localization;Tung tree;β-Hydroxyacyl-acyl carrier protein dehydratase
摘要：
Beta-hydroxyacyl-acyl carrier protein dehydratase (HAD) catalyzes the formation of enoyl-[ACP] from beta -hydroxylacyl-[ACP] and plays a key role in determining fatty acid composition during the process of oil biosynthesis. In this study, only one HAD gene (GenBank KU358903) was identified and isolated from tung tree (Vernicia fordii Hemsl.). The VfHAD contained an open reading frame (ORF) of 663 bp, encoding a polypeptide of 220 amino acid residues. It shared 86, 85, and 83% amino acid identities with the HADs of Hevea brasiliensis, Jatropha curcas, and Ricinus communis, respectively. The VfHAD exhibited all essential catalytic sites and the characteristic “hot-dog” structure of FabZ protein. Quantitative real-time PCR (qRT-PCR) analysis showed that VfHAD mRNA was detected in all tung tree organs, but its transcript was the most abundant in tung seeds at 20 weeks after flowering when oil rapidly accumulated. The VfHAD was localized in chloroplast. Heterologous expression of VfHAD significantly increased C16 fatty acids content in Escherichia coli, yeast, and tobacco. This study suggested that VfHAD may play a key role in fatty acid biosynthesis in tung seeds and could be a useful target for genetic improvement of oil quality. © 2018 Elsevier B.V.
语种：
英文
展开
油茶转运蛋白基因ABCB26的克隆及表达分析
作者：
余姝姝;周俊琴;卢梦琪;刘懿瑶;杨进;...
期刊：
植物生理与分子生物学学报 ,2019年55(11):1664-1672 ISSN：1671-3877
通讯作者：
Tan, X.-F.
作者机构：
[刘懿瑶; 周俊琴; 余姝姝; 卢梦琪; 谭晓风; 杨进] 中南林业科技大学
通讯机构：
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
关键词：
油茶;基因克隆;表达;亚细胞定位
摘要：
以油茶(Camellia oleifera) ‘华硕'品种为研究对象,通过RT-PCR克隆得到腺苷三磷酸结合盒转运蛋白ABCB基因家族的一个成员,命名为CoABCB26。该基因的开放阅读框(ORF)为1 491 bp,共编码496个氨基酸。序列比对发现,油茶CoABCB26基因与茶树(Camellia sinensis)的CsABCB26基因亲缘关系最近,相似性达99%,且具有ABC转运蛋白3个特殊结构域: Walker A结构域、ABC标签序列和Walker B结构域。CoABCB26转运蛋白为稳定的疏水性蛋白,相对分子质量为55.88 kDa,理论等电点为8.69,具有2个跨膜结构域,但不具有信号肽;其二级结构主要由α螺旋和无规卷曲两种元件构成。实时定量PCR结果表明, CoABCB26基因在芽和花粉中的表达量较高,且在授粉78 h时, ‘华硕'ב华金'异交组合的花柱中表达量最高,表明CoABCB26基因在油茶后期自交不亲和过程中发挥了重要作用。亚细胞定位发现油茶CoABCB26蛋白主要分布在细胞膜上,可能参与物质的跨膜运输。
语种：
中文
展开
青钱柳组织培养及快速繁殖
作者：
吴玲利;韩航;曾艳玲;谭晓风;李建安;...
期刊：
植物生理与分子生物学学报 ,2019年55(1):61-68 ISSN：1671-3877
通讯作者：
Li, Z.
作者机构：
中南林业科技大学经济林培育与保护教育部重点实验室,长沙410004;中南林业科技大学经济林培育与利用湖南省协同创新中心,长沙410004;中南林业科技大学经济林育种与栽培国家林业局重点实验室,长沙410004;[李泽; 韩航; 曾艳玲; 谭晓风; 吴玲利; 李建安] 中南林业科技大学
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Education Ministry, Central South University of Forestry and Technology, Changsha, China
关键词：
青钱柳;增殖;组织培养;壮苗
摘要：
以青钱柳(Cyclocarya paliurus)种胚萌发的无菌苗为外植体,研究植物生长调节剂对试管苗增殖及植株再生的影响,结果表明:较适宜青钱柳增殖的基本培养基为1/2MS培养基;最佳继代增殖培养基为1/2MS+3.0 mg·L~(-1) 6-苄氨基腺嘌呤(6-BA)+0.05 mg·L~(-1)吲哚-3-乙酸(IAA),增殖系数可达7.5;最佳壮苗伸长培养基为1/2MS+0.5 mg·L~(-1) IAA+5.0 mg·L~(-1)赤霉素(GA3);最佳生根培养基为1/2MS+0.5 mg·L~(-1)吲哚-3-丁酸(IBA)+0.5 mg·L~(-1) (萘乙酸) NAA,生根率可达32.0%。
语种：
中文
展开
Expression Profiles of a Tung Tree Phosphate Transporter cDNA and Structural Characteristics of the Encoded Protein
作者：
Zhou, Junqin* ;Yuan, Jun* ;Long, Hongxu* ;Tan, Xiaofeng*
期刊：
Cytology and Genetics ,2019年53(2):162-168 ISSN：0095-4527
通讯作者：
Zhou, Junqin;Yuan, Jun;Long, Hongxu;Tan, Xiaofeng
作者机构：
[Zhou, JQ; Yuan, Jun; Long, Hongxu; Tan, Xiaofeng; Zhou, Junqin] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Nurturing & Protect, Natl Minist Educ, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhou, JQ; Yuan, J; Long, HX; Tan, XF] C;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Nurturing & Protect, Natl Minist Educ, Changsha 410004, Hunan, Peoples R China.
关键词：
1, gene cloning, gene expression;Vernicia fordii, phosphate transporter, VfPT1
摘要：
Plant phosphate transporters mediate phosphate acquisition, translocation, and recycling. A Vernicia fordii cDNA encoding a phosphate transporter was cloned using a reverse transcription polymerase chain reaction (PCR) and rapid amplification of cDNA ends. The resulting cDNA named Vernicia fordii Phosphate Transporter-1;1 (VfPht1;1) encodes a predicted protein consisting of 533 amino acids. This protein, which was predicted to contain 12 transmembrane domains, is most similar (89.9%) to a Ricinus communis predicted protein. The quantitative real-time PCR data revealed that VfPht1;1 is most highly expressed in mature leaf petioles. It is also highly expressed in seeds collected in June, when developing fruits require a large amount of phosphates. Our observations suggest that VfPht1;1 may have diverse functions related to phosphate uptake and translocation.
语种：
英文
展开
不同光照对油桐幼苗生长、光合日变化及叶绿素荧光参数的影响
作者：
石凯;李泽;张伟建;何潇;曾艳玲;...
期刊：
中南林业科技大学学报 ,2018年38(8):35-42,50 ISSN：1673-923X
作者机构：
中南林业科技大学 经济林培育与保护省部共建教育部重点实验室,湖南 长沙 410004;中南林业科技大学 经济林育种与栽培国家林业局重点实验室,湖南 长沙 410004;[石凯; 李泽; 张伟建; 何潇; 曾艳玲; 谭晓风] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室, 湖南, 长沙, 410004 中南林业科技大学, 经济林育种与栽培国家林业局重点实验室, 湖南, 长沙, 410004
关键词：
油桐;净光合速率;光合特性;叶绿素荧光
摘要：
为探讨不同光照强度对油桐幼苗生长、叶片气体交换及叶绿体荧光参数的影响,采用盆栽试验,设置了4种光照条件光强度100%(L1)、光强度75%(L2)、光强度50%(L3)、光强度20%(L4),分别在遮阴2个月、3个月测定了幼苗的生长、叶绿素含量、光合日变化及叶绿素荧光参数的变化。结果表明:(1)在L3的透光条件下,油桐幼苗苗高显著增加,但地径随着遮阴强度的增加而逐渐降低(P < 0.05);(2)随着光照强度的降低,叶绿素a(Chl a)、叶绿素b(Chl b)和叶绿素总量(Chl a+b)先增加后降低,说明适当的遮阴有利于叶绿素的合成;(3)随着光照强度的降低,油桐的净光合速率(P_n)、气孔导度(G_s)、最大光化学效率(F_v/F_m)、表现光合电子传递速率(ETR)及蒸腾速率(T_r)逐渐减小,且在L3的透光条件下显著低于对照(P < 0.05),胞间CO2浓度(Ci)无明显变化规律,在L2的透光条件下,P_n的降低主要是气孔因素引起的,在L3的透光条件下,P_n的降低主要是由气孔因素和非气孔因素共同引起的,而在L4的透光条件下,P_n的降低主要是由光合机构活性损伤的非气孔因素引起的。
语种：
中文
展开
升级打造湖南千亿元油茶产业调查研究
作者：
谭晓风;管天球;袁军
期刊：
经济林研究 ,2018年36(3):1-4 ISSN：1003-8981
作者机构：
中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学经济林育种与栽培国家林业局重点实验室;中南林业科技大学经济林培育与利用湖南省高校“2011”协同创新中心;[管天球] 湖南应用技术学院;[袁军; 谭晓风] 中南林业科技大学
关键词：
湖南;油茶产业;存在问题;产业升级;发展措施
摘要：
本研究从国家政策支持、湖南省油茶栽培规模、栽培品种潜力、产品开发前景、市场需求、技术能力等方面综合分析了湖南打造千亿元油茶产业的可能性;全面解析了当前湖南油茶产业发展存在的品种良莠不齐、老残油茶林面积大、栽培管理技术应用不到位、茶油及副产物深度利用不够、地方茶油品牌缺乏、财政支持力度不够、产业发展模式创新不到位、产业发展环境有待改善等问题;系统提出了切实制定湖南千亿元产业发展目标和政策措施、全面优化品种、规范油茶优质丰产栽培技术、全面实施油茶低产林改造、充分发挥深加工产品的增值效益、提升机械作业水平、着力打造湖南油茶产品、创新产业发展模式、加大培养人才力度等加快实施转型升级、规划打造湖南千亿元油茶产业的综合措施。
语种：
中文
展开
油桐4个优良家系主要经济性状分析
作者：
李文莹;刘美兰;张琳;谭晓风;张帆航;...
期刊：
经济林研究 ,2018年36(2):29-34 ISSN：1003-8981
作者机构：
[李文莹; 刘美兰; 张琳; 谭晓风; 张帆航] 中南林业科技大学经济林培育与保护省部共建教育部重点试验室,湖南长沙,410004;[王自重] 湖南省永州市森林资源监测中心,湖南永州,425000
关键词：
油桐;优良家系;经济性状;桐油;理化性质
摘要：
为给油桐良种选育提供参考依据,采用田间调查的方法,从湖南省湘西自治区永顺县青坪镇油桐种质资源收集保存库,选取4个油桐优良家系P005、P007、P009、P012作为试验材料,采用化学分析法、索式提油法、色谱分析等方法,对此4个优良家系的果实性状、种仁性状、桐油油脂脂肪酸成分及油脂的理化性质进行了比较分析.测定结果表明:家系P005的不饱和脂肪酸含量最高;家系P007的桐酸含量最高;家系P009的桐油品质最好;家系P012的出油率最高,其综合性状最好.4个优良家系各性状指标值各有高低,综合分析各项指标后认为,优良家系P005和P012均有较高的生产应用价值,不论是作为良种选育的原始材料还是直接用于生产繁殖,都是较好的选择.
语种：
中文
展开
油茶钙调素基因的cDNA克隆与表达分析
作者：
王保明;谭晓风;胡孝义;石明旺;莫华
期刊：
中南林业科技大学学报 ,2018年38(5):70-78 ISSN：1673-923X
作者机构：
湖南应用技术学院农林科技学院, 湖南, 常德, 415000;中南林业科技大学, 经济林培育与保护教育部重点实验室, 湖南, 长沙, 410004;中南林业科技大学, 经济林培育与利用湖南省高校“2011”协同创新中心, 湖南, 长沙, 410004;[王保明] 湖南应用技术学院农林科技学院, 湖南, 常德, 415000 中南林业科技大学, 经济林培育与保护教育部重点实验室, 湖南, 长沙, 410004 中南林业科技大学, 经济林培育与利用湖南省高校“2011”协同创新中心, 湖南, 长沙, 410004;[谭晓风; 胡孝义; 石明旺] 中南林业科技大学, 经济林培育与保护教育部重点实验室, 湖南, 长沙, 410004 中南林业科技大学, 经济林培育与利用湖南省高校“2011”协同创新中心, 湖南, 长沙, 410004
关键词：
油茶;钙调素;理化性质;荧光定量PCR;表达模式
摘要：
在构建油茶近成熟种子cDNA文库的基础上,分离鉴定了油茶钙调素基因CoCaM1、CoCaM2的cDNA序列(GenBank登录号:EU856536和FJ649316),它们的长度分别为953 bp和1024 bp,均含有447 bp的开放读码框,编码的149个氨基酸完全相同;它们编码区的核苷酸序列高度一致,仅有25个碱基替代,证实了“多个基因编码一种蛋白”的假设。该蛋白含有19种氨基酸,属于酸性亲水性蛋白,理论等电点4.10,相对分子量16.83 kDa;它含有EF-手臂、半胱氨酸等结构域。该蛋白的亲水性/疏水性、柔性、抗原性具有较好的一致性,并表现高度的柔性。Blast及遗传进化分析表明:该蛋白的氨基酸序列与其它植物钙调蛋白的氨酸序列同源性较高。油茶CoCaM1在花芽到子房形成中的表达量较高;而CoCaM2在果实形成、膨大、油脂积累过程中的表达量较多,而在叶片和成熟种子中较少。这表明它们在花芽发育、果实形成、种子油脂合成积累中发挥着不同的调控作用。
语种：
中文
展开
采后晾晒对油茶种仁油脂产量及组分的影响
作者：
曾艳玲;颜亚丹;谭晓风;何超银;杨睿
期刊：
植物生理与分子生物学学报 ,2018年54(2):316-324 ISSN：1671-3877
通讯作者：
Zeng, Y.-L.
作者机构：
[曾艳玲; 颜亚丹; 谭晓风; 何超银; 杨睿] 中南林业科技大学, 经济林培育与保护教育部重点实验室;[曾艳玲; 颜亚丹; 谭晓风; 何超银; 杨睿] 经济林育种与栽培国家林业局重点实验室;[曾艳玲; 颜亚丹; 谭晓风; 何超银; 杨睿] 经济林培育与利用湖南省协同创新中心, 长沙, 410004
关键词：
油茶;晾晒;光质;油脂含量;脂肪酸
摘要：
油茶(Camellia oleifera)是我国大力推广的优质油料树种。本研究分别取花后150和170 d油茶果实,比较新鲜提取、自然晾晒及不同光质晾晒后种仁含油率及脂肪酸含量,旨在为合理采收处理提供科学依据。结果表明,晾晒能够显著提高花后150 d油茶种仁出油率,其中‘华鑫'种仁出油率的增加达5.35%。覆盖蓝色膜晾晒最有利于花后150 d的‘华硕'油茶油脂采后合成,种仁出油率高达52.42%。晾晒对花后170 d油茶种仁出油率影响不大。无论晾晒与否,脂肪酸成分不变,花后170 d较花后150 d油茶种仁中硬脂酸和油酸含量均有增加,但是品种间差异不显著。
语种：
中文
展开
油茶主栽品种光合特性的比较
作者：
吴方圆;李泽;刘繁灯;王慧;石凯;...
期刊：
经济林研究 ,2018年36(2):7-13 ISSN：1003-8981
作者机构：
中南林业科技大学经济林育种与栽培国家林业局重点实验室;中南林业科技大学经济林培育与保护省部共建教育部重点实验室;中南林业科技大学经济林培育与利用湖南省协同创新中心;江西省林业科学研究院;[吴延旭] 赣州市林业科学研究所
会议名称：
中国林学会经济林分会2017年学术年会
会议时间：
2017-08-24
会议地点：
哈尔滨
会议论文集名称：
中国林学会经济林分会2017年学术年会论文集
关键词：
油茶;品种;光合特性
摘要：
为筛选高光效的油茶主栽品种,从而为油茶品种评价和引种栽培提供理论依据。利用Li-6400xt便携式光合仪对15个油茶主栽品种的光合日变化、光响应曲线、CO2响应曲线进行测定比较。结果表明:（1）油茶品种间日变化净光合速率均值、光响应净光合速率均值、最大净光合速率、暗呼吸速率、表观量子效率、光补偿点、光呼吸速率、羧化效率、CO2补偿点存在差异;（2）相关性分析表明最大净光合速率与表观量子效率呈显著正相关;暗呼吸效率与表观量子效率呈显著正相关、与光补偿点呈极显著正相关。光呼吸速率与羧化效率呈极显著正相关。羧化效率与CO2补偿点呈显著负相关。所以,在15个油茶主栽品种中,‘华鑫’‘华金’‘赣兴48号’‘湘林210号’‘长林4号’5个品种光合效率较高。
语种：
中文
展开
不同温度下油菜素内酯对油桐幼苗生长及生理指标的影响
作者：
张帆航;李泽;谭晓风;李文莹;杨超臣;...
期刊：
经济林研究 ,2018年36(3):17-24 ISSN：1003-8981
作者机构：
中南林业科技大学 经济林培育与保护教育部重点实验室,湖南长沙 410004;中南林业科技大学 经济林培育与利用湖南省协同创新中心,湖南长沙 410004;[杨超臣; 李泽; 张帆航; 李文莹; 李艳丽; 谭晓风] 中南林业科技大学
关键词：
千年桐;三年桐;油菜素内酯;温度胁迫;栅栏组织
摘要：
选取1年生油桐幼苗为试验材料,研究不同温度下油菜素内酯（BR）对油桐幼苗生长及生理特性的影响。结果表明：8℃和38℃2种不同温度胁迫下,2个油桐物种幼苗生长减缓,叶片及栅栏组织变薄,叶绿素含量和SPAD值下降,过氧化物酶（POD）和超氧化物歧化酶（SOD）活性升高,可溶性糖和可溶性蛋白含量增加,丙二醛（MDA）含量上升。相同温度下,叶片喷施BR可以使油桐幼苗生长量增加,叶片及叶片栅栏组织加厚,叶绿素含量和SPAD值上升、POD和SOD活性增加,可溶性糖和可溶性蛋白含量提升,叶片相对含水率（RWC）增加,MDA含量降低。说明BR可以减轻温度胁迫对油桐叶片的抑制程度,增强油桐叶片的生理功能,提高叶片内各种抗氧化酶活性,降低MDA对细胞膜的毒害作用,进而增加油桐幼苗的抗热性和抗寒性,加快油桐幼苗有机物的积累,最终使生物量增加。
语种：
中文
展开
Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L.
作者：
Peng, Dan;Tan, Xiaofeng;Zhang, Lin;Yuan, Deyi;Lin, Jianzhong;...
期刊：
Breeding Science ,2018年68(3):326-335 ISSN：1344-7610
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Liu, Xuanming; Lin, Jianzhong] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] C;[Zhou, Bo] H;[Zhou, Bo] F;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
关键词：
branching;seed yield;OsPAT15;B. napus L.;sulfur acyl transferase
摘要：
Branching is a predominant element in the plant architecture of Brassica napus L. and represents an important determinant of seed yield. OsPAT15 (OsDHHC1), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the OsPAT15 gene from the monocot rice into the dicot B. napus L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene OsPAT15 was determined to have sulfur acyl transferase activity in the akr1Δ yeast mutant in a complementation experiment. Heterologously expressing OsPAT15 transgenic B. napus L. plants were obtained using the Agrobacterium-mediated floral-dip transformation method. As anticipated, OsPAT15 transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, OsPAT15 transgenic plants had increased primary branches (1.58–1.76-fold) and siliques (1.86–1.89-fold), resulting in a significant increase in seed yield (around 2.39– 2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene OsPAT15 in B. napus L. could be used to increase seed yield and produce excellent varieties. © 2018, Japanese Society of Breeding. All rights reserved.
语种：
英文
展开
Enhancing freezing tolerance of Brassica napus L. by overexpression of a stearoyl-acyl carrier protein desaturase gene (SAD) from Sapium sebiferum (L.) Roxb.
作者：
Peng, Dan;Zhou, Bo* ;Jiang, Yueqiao;Tan, XiaoFeng;Yuan, DeYi;...
期刊：
Plant Science ,2018年272:32-41 ISSN：0168-9452
通讯作者：
Zhou, Bo
作者机构：
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
通讯机构：
[Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.
关键词：
Freezing tolerance;Sapium sebiferum (L.) Roxb.;Stearoyl-acyl carrier protein desaturase;Unsaturated fatty acid
摘要：
Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W10 by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L. © 2018 Elsevier B.V.
语种：
英文
展开
