期刊论文

中文

林业科学

1001-7488

2009

45

11

36-43

10.3321/j.issn:1001-7488.2009.11.007

Supported by the Youth Foundation of Education Bureau of Hunan Province（1014586） the Key Project of State Forestry Administration（2006-12） the Important Project of Ministry of Education

本校为第一机构

以5个已知S基因型的中国白梨品种为试材,设计分别对应于梨SFBB-alpha,SFBB-beta和SFBB-gamma 基因的3对引物对这5个品种进行基因组PCR扩增.结果仅引物组合PSFBG-F/PSFBG-R从‘金花'(S_(13)S_(18)),‘金花四号'((S_(13)S_(18))和‘鹅梨'((S_(13)S_(34))中扩增出一约1 300 bp的条带,经回收、克隆、测序后,鉴定其为SFBB-gamma基因,命名为PbSFBB13-gamma(Pyrus bretschneideri SFBB13-gamma),GenBank登录号为EU081892.逆转录PCR结果表明PbSFBB13-gamma仅在花粉中特异表达;序列分析表明该基因不含内含子,编码区含1 191个核苷酸,编码396个氨基酸,预测分子质量与等电点分别为45.4 ku、4.63.PbSFBB13-ga...

Five Chinese White Pear（Pyrus bretschneideri）cultivars,with known S-genotypes,were used in this study.The genomic DNAs were extracted and amplified by PCR with three pairs of primers corresponding to pear SFBB-alpha,SFBB-beta and SFBB-gamma genes,respectively.Results showed that only one primer pair PSFBG-F/PSFBG-R successfully amplified a fragment of approximately 1 300 bp from ＇Jinhua＇（S_ 13S_ 18）,＇Jinhuasihao＇（S_ 13S_ 18）and ＇Eli＇（S_ 13S_ 34）corresponding to pear SFBB-gamma gene.This gene was named PbSFBB13-gamma（Pyrus bretsch...