There are two tough problems in tissue culture of plants. One is high cost, another is low survival rate of transplant. In order to reduce the cost of tissue culture with agar, and to increase survival rate of transplant, another kind of support in culture medium instead of agar is needed, which is cheap and can be washed down easily. We respectively put the buds of Gerbera jamesonii in nutrient solution (MS+BA 15 mg.L-1 +IBA 0.2 mg.L-1) with three kinds of medium in vitro to induce to root, which are grit, sponge, and agar. And then rooted plantlets were transplanted from culture bottle to fl...
