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cDNA Cloning, Sequence Analysis and Homology Modeling of S20-RNase in Chinese White Pear (Pyrus bretschneideri Rehd.)

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成果类型:
期刊论文、会议论文
作者:
Yuan Deyi*;Zhang Lin;Tan Xiaofeng;Shen Yan
通讯作者:
Yuan Deyi
作者机构:
[Tan Xiaofeng; Yuan Deyi; Zhang Lin] Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.
[Shen Yan] Cent South Univ Forestry & Technol, Sch Adult Educ, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Yuan Deyi] C
Cent South Univ Forestry & Technol, Coll Forestry, Forestry Minist, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.
语种:
英文
关键词:
Chinese white pear;gametophytic self-incompatibility;rapid amplification of cDNA ends;S-20-RNase;three-dimension (3D) structure
期刊:
2009 3RD INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICAL ENGINEERING, VOLS 1-11
ISSN:
2151-7614
年:
2009
页码:
1-4
会议名称:
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
会议论文集名称:
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
会议时间:
June 2009
会议地点:
Beijing, China
出版者:
IEEE
ISBN:
978-1-4244-2901-1
基金类别:
agricultural achievement transforming programs of science and technology department [2008GB2D200219]; Scientific Research Fund of Central South University of Forestry Technology [2008007B]
机构署名:
本校为第一且通讯机构
院系归属:
林学院
材料科学与工程学院
摘要:
To elucidate the function of S-allele and the possible molecular mechanism of gametophytic self-incompatibility (GSI) in Chinese white pear (Pyrus bretschneideri Rehd.), full length cDNA encoding S 20 -RNase was cloned by rapid amplification of cDNA ends (RACE) approach from the cultivar 'Donghuang' (S 8 S 20 ), and analyzed by bioinformatics method. S 20 -RNase displayed typical sequence features of pear S 20 -RNases, i.e. five conserved regions and a hypervariable (HV) region. The predicted three-dimension (3D) structure of the S 20 -RNase was similar to that of 53-RNase of Japanese pear (P....

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