期刊论文

中文

林业科学

1001-7488

2008

44

10

42-48

10.3321/j.issn:1001-7488.2008.10.008

Foundation project： Supported by the Key Project of State Forestry Administration （2006 - 12） and Scientific Research Fund of Central South University of Forestry ＆ Technology （2008007B）.

本校为第一机构

砂梨是重要的经济树种,表现出典型的配子体自交不亲和性,在生产和育种上需鉴定品种的S基因犁以确定品种间的亲和性.选取7个砂梨品种为试验材料,使用梨S-RNase(S基因)通用引物进行基因组PCR扩增,产物通过1.8%的琼脂糖凝胶电泳分析.结果表明:‘楚比香'等4个品种中产生了预期的2条电泳条带,而其他3个品种都只产生1条带产物,通过6%的聚丙烯酰胺电泳对该3个品种的PCR产物进一步分析,结果产物被成功分离.将7个品种中分离到的14个条带分别回收、克隆、测序及序列分析,从中鉴别出10个具有梨S-RNase基因序列特征的S基因,其中‘政和大雪梨'中494 bp的基因片段被鉴定为新的S基因,暂命名为S43-RNase(GenBank...

Pyrus pyrifolia is a commercially important fruit tree which exhibits gametophytic seLf-incompatibility （GSI）. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, seven cuhivars of P. pyrifolia were used for S-genotype analysis by PCR-based molecular method with primers designed from conserved sequences of known pear S-RNases. Amplified products were analyzed by 1.8 % agarose gel electrophoresis. In each of the four cul...