Characterization of the key aroma compounds of a sweet rice alcoholic beverage fermented with Saccharomycopsis fibuligera
作者：
Yang, Yurong;Zhong, Haiyan;Yang, Tao* ;Lan, Caihong;Zhu, He
期刊：
Journal of Food Science and Technology ,2021年58(10):3752-3764 ISSN：0022-1155
通讯作者：
Yang, Tao
作者机构：
[Lan, Caihong; Yang, Tao; Yang, Yurong; Zhu, He; Zhong, Haiyan] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Byprod Deep Proc, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Yang, Tao] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Rice & Byprod Deep Proc, Changsha 410004, Hunan, Peoples R China.
关键词：
Drug products;Mass spectrometry;Odors;Volatile organic compounds;Wine;Yeast;Gas chromatography-mass spectrometry;Head-space solid-phase microextraction;Key aroma compounds;Non-saccharomyces yeasts;Odour activity value (OAV);Quantitative descriptive analysis;Quantitative result;Volatile compounds;Gas chromatography
摘要：
This study aims to examine the effect of the non-Saccharomyces yeast Saccharomycopsis fibuligera on the sensory quality and flavour characteristics of a sweet rice alcoholic beverage. The strain S. fibuligera was isolated from a traditional Chinese hand-made starter with the purpose to improving sweet rice wine fragrance. Here, sweet rice wines were produced by six combinations of three species of fermentation strains, including S. fibuligera, Rhizopus and Saccharomyces cerevisiae, for evaluation. The study results showed significant diversities within these rice wines based on indicators including the score of quantitative descriptive analysis and volatile variety and content as well as odour activity value (OAV). Quantitative results showed that 43 volatile compounds were identified by headspace-solid phase microextraction with gas chromatography-mass spectrometry among samples. Based on the principal component analysis and OAV calculation, the two samples (S-2 and S-3) fermented with S. fibuligera and Rhizopus possessed high scores and were distinguished from the others, and ethyl butanoate, ethyl hexanoate, β-phenylethyl alcohol and 1-octen-3-one with high OAVs were responsible for the key aroma of sweet rice wine fermented with S. fibuligera. Co-inoculating S. fibuligera, Rhizopus or/and S. cerevisiae generated more pleasant aroma compounds in a sweet rice alcoholic beverage than when inoculated individually. © 2020, Association of Food Scientists & Technologists (India).
语种：
英文
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响应面法优化风味米曲制备工艺
作者：
吴健;何伟;王建成;杨玉蓉;蓝彩红;...
期刊：
中国酿造 ,2020年39(7):120-124 ISSN：0254-5071
作者机构：
中南林业科技大学食品科学与工程学院;[何伟] 长沙市食品药品信息与审评认证中心;[王建成] 四川邛崃金六福崖谷生态酿酒有限公司;[蓝彩红; 吴健; 杨涛; 刘盛钢; 杨玉蓉] 中南林业科技大学
关键词：
产香酵母;米根霉;风味米曲;制备工艺;优化
摘要：
用异常维克汉逊酵母(Wickerhamomyces anomalus)、扣囊复膜酵母(Saccharomycopsis fibuligera)、克鲁维毕赤酵母(Pichia kudriazevii)、酿酒酵母(Saccharomyces cerevisiae)制备复配酵母,与纯种米根霉菌共培养制备风味米曲,采用单因素试验及响应面法优化制曲工艺.并以市售小曲为对照,对风味米曲进行了质量指标检测与半固态发酵制备米香型白酒验证.结果 表明,最优制备工艺为酵母接种量1.0％,培养时间73 h,培养温度30℃.在此优化条件下,试饭的酯含量为3.65 g/L.最优工艺制备的风味米曲水分含量为10.58％,糖化力为423.77 U/g,发酵力为2.24 g/(g·72 h).经半固态法实际酿酒后,酒体总酯含量达到了6.87 g/L,总酸含量与感官评分分别为1.63 g/L、92分,具备米香型白酒典型风格,且风味明显提升.
语种：
中文
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Systematic evaluation of the physicochemical properties and the volatile flavors of yak meat during chilled and controlled freezing-point storage
作者：
Sun, Shuguo;Zhao, Juanhong;Luo, Zhang;Lin, Qinlu;Luo, Feijun;...
期刊：
Journal of Food Science and Technology ,2020年57(4):1351-1361 ISSN：0022-1155
通讯作者：
Yang, Tao
作者机构：
[Luo, Feijun; Yang, Tao; Zhao, Juanhong; Sun, Shuguo; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha Engn Res Ctr Food Storage & Preservat,Hu, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;[Luo, Zhang] Tibet Agr & Anim Husb Univ, Coll Food Sci, Lingzhi 860000, Tibet, Peoples R China.;[Luo, Feijun] Univ Cambridge, Addenbrookes Hosp, Dept Pathol, Cambridge, England.
通讯机构：
[Yang, Tao] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha Engn Res Ctr Food Storage & Preservat,Hu, Hunan Key Lab Processed Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.
关键词：
Yak meat;Deterioration index;Volatile compounds;Headspace solid-phase microextraction
摘要：
In this study, the physicochemical properties (total volatile basic nitrogen (TVB-N), pH, and peroxide value) and the volatile flavors of yak meat were systematically evaluated during chilled (0 degrees C) and controlled freezing-point (- 2 degrees C) storage. The TVB-N reached 15.21 mg/100 g after 18 days of storage at 0 degrees C, which exceeded the secondary freshness value according to the Chinese national standard. For storage at - 2 degrees C, the TVB-N did not exceed 15 mg/100 g until 24 days. Compared with storage at 0 degrees C, the samples stored at - 2 degrees C had a slower rate of increase in TVB-N, pH, and peroxide values. The changes in volatile compounds in yak meat during storage at - 2 degrees C and 0 degrees C for 24 days were investigated using headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS). The correlations between the changes in the volatile compound contents and meat quality deterioration revealed significant negative correlations (r(min) = 0.902, p < 0.05) between some aldehyde flavor components (nonanal, heptanal, benzaldehyde, decanal, and myristal) and TVB-N in the samples stored at controlled freezing-point and chilled temperatures. The decreases in nonanal, benzaldehyde, and myristal contents in yak meat followed zero order reaction kinetics. This result indicated, because of the highly selective and sensitive colorimetric detection method, that volatile compounds can effectively predict the decay in quality of yak meat stored at low temperature in advance. Thus, based on physicochemical and volatile flavor analyses, a new method is proposed to investigate the storage and preservation of yak meat.
语种：
英文
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Advances in Nutritional Function of Extracellular Polysaccharides from Lactic Acid Bacteria
作者：
Zhu, He;Yang, Tao;Xing, Yanxia;Chang, Guifang*
期刊：
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS ,2020年30(1):345-353 ISSN：0798-2259
通讯作者：
Chang, Guifang
作者机构：
[Yang, Tao; Zhu, He] Cent South Univ Forestry & Technol, Dept Food Sci & Engn, Changsha 410000, Peoples R China.;[Xing, Yanxia; Zhu, He; Chang, Guifang] Shandong Agr & Engn Univ, Dept Food Sci & Engn, Jinan 250100, Peoples R China.
通讯机构：
[Chang, Guifang] S;Shandong Agr & Engn Univ, Dept Food Sci & Engn, Jinan 250100, Peoples R China.
关键词：
Lactic Acid Bacteria;Extracellular Polysaccharide;Nutritional Effect;Promoting Transformation
摘要：
Lactic acid bacteria are recognized as food safety microorganisms, extracellular polysaccharide is a kind of carbohydrate produced and secreted by Lactobacillus during its growth and metabolism. There are many probiotic functions, such as immune regulation, anti-cancer, anti-oxidation, regulation of intestinal microecological balance and so on. In this paper, the research progress on the probiotic function of extracellular polysaccharides from Lactobacillus was reviewed; the mechanism of action of extracellular polysaccharides was briefly summarized. To study the transformation of splenic lymphocyte in vitro by six polysaccharide samples of EPSc, EPSw, EpSL, Epss, EPSm and EPSF. The results showed that a certain concentration of polysaccharide could promote the transformation of resting lymphocytes and ConA-activated lymphocytes, and the effect of 6 polysaccharide samples on lymphocyte transformation was consistent with their inhibition on 5180. EPS has no inhibitory effect on immune cells; it has the function of improving cellular immunity in vitro. The immune activity of 6 polysaccharide samples in vitro was still dose-dependent at the concentration of 10100 g/mL. The inhibitory effect of MFL02-8 extracellular polysaccharide co-culture supernatant with resting and ConA-activated splenic lymphocytes on 5180 tumor cells was enhanced, these results suggest that polysaccharides can promote the secretion of cytokines by lymphocytes, plays an anti-cancer role. The amount of cytokines increased in co-culture of polysaccharides and lymphocytes activated by ConA. At the same time, the existing problems and future research directions are discussed, and the aim of this study is to provide theoretical reference for further research and production application of Lactobacillus extracellular polysaccharides in animal nutrition.
语种：
英文
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Regularity and Molecular Mechanism of the Effect of Pueraria Lobata on Alcoholic Headache
作者：
Zhu, He;Xing, Yanxia;Yang, Tao* ;Chang, Guifang*
期刊：
Acta Microscopica ,2020年29(1):229-238 ISSN：0798-4545
通讯作者：
Yang, Tao;Chang, Guifang
作者机构：
[Yang, Tao; Zhu, He] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Changsha 410000, Peoples R China.;[Xing, Yanxia; Zhu, He; Chang, Guifang] Shandong Agr & Engn Univ, Dept Food Sci & Engn, Jinan 250100, Peoples R China.
通讯机构：
[Yang, Tao] C;[Chang, Guifang] S;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & By Prod Deep Proc, Changsha 410000, Peoples R China.;Shandong Agr & Engn Univ, Dept Food Sci & Engn, Jinan 250100, Peoples R China.
关键词：
Alcoholic Headache;Molecular Mechanism;Pueraria Hangover;Pueraria Research
摘要：
At present, there is still no effective therapeutic drug for the treatment of alcoholic headache in the domestic and international markets. The research on the effect of pueraria hangover is the determination of conventional indicators such as anti-oxidation, hypolipidemic and liver function, and lack of in-depth discussion on its mechanism of action. The purpose of this paper is to analyze the effects of puerarin on relieving alcoholic headache by observing the neurobehavioral changes and changes in blood alcohol concentration in mice, and to understand the basic situation of alcoholic headache and alcoholic liver disease and the different radix puerariae concentration on mouse blood alcohol. The effects of concentration, enzyme concentration and mouse behavior were compared to study the effects of puerarin on oxidative stress, lipid metabolism and inflammatory signaling in drunken mice, and to explore the mechanism of puerarin. Using the method of this paper, through the analysis of experimental data, we learned the role and molecular mechanism of Pueraria lobata in relieving alcoholic headache, and found that pueraria lobata plays an important role in relieving alcoholic headache. The high concentration of Pueraria germination ability is 80%, through theory and experiment. The data were combined to analyze the high concentration of puerarin dose group for headache and blood lipid reduction of 53%. The results showed that puerarin has an important role in relieving alcoholic headache. It can be used for alcohol hangover, hangover ability and increase with puerarin concentration. To provide a reference for further effective treatment of alcoholic liver disease and the development of effective therapeutic drugs. © 2020, Interamerican Society for Electron Microscopy (CIASEM). All rights reserved.
语种：
英文
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酿酒酵母与产香酵母发酵特性及其相互作用规律
作者：
吴健;何伟;王建成;杨玉蓉;蓝彩红;...
期刊：
食品与发酵工业 ,2020年46(20):78-87 ISSN：0253-990X
作者机构：
中南林业科技大学 食品科学与工程学院,湖南 长沙,410000;长沙市食品药品信息与审评认证中心,湖南 长沙,410000;四川邛崃金六福崖谷生态酿酒有限公司,四川 成都,611530;广东德庆无比养生酒业有限公司,广东 肇庆,526600;[蓝彩红; 吴小霞; 吴健; 杨涛; 刘盛钢; 杨玉蓉] 中南林业科技大学
关键词：
酿酒酵母;产香酵母;发酵特性;相互作用;风味
摘要：
为提高米香型白酒的品质,将筛选得到的克鲁维毕赤酵母(Pichia kudriazevii)、异常维克汉逊酵母(Wickerhamomyces anomalus)、扣囊复膜酵母(Saccharomycopsis fibuligera) 3株产香酵母与1株酿酒酵母(Saccharomyces cerevisiae)作为供试菌株,研究了其菌株形态、生长周期、抗逆性以及发酵特性,并探索了菌株间的相互作用对菌体生长与代谢的影响规律。结果显示,各菌株进入稳定期的时间存在明显差异。在抗逆性方面,4株菌均表现出良好的耐热性、耐酸性、乙醇耐受性与糖耐受性。产酸、产酯、产乙醇能力最强的菌株分别是S.fibuligera、P.kudriazevii、S.cerevisiae。相互作用方面,S.cerevisiae会抑制其余3株产香酵母的生长(P <0.05),而产香酵母对S.cerevisiae的生长无明显影响;此外,P.kudriazevii会显著抑制W.anomalus(P <0.05)。4株菌最优的接种比例为106 ∶106 ∶106 ∶106 CFU/mL,在接种3株产香酵母8 h后再接种S.cerevisiae,风味物质的含量最优。研究结果表明,供试菌株具备生物增香、提高酒体品质的良好潜力。
语种：
中文
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A portable device enabling fluorescent-to-electric resistant transduction for selective Cr3+ detection based on its slow ligand bind kinetics
作者：
Li, Wang;Chen, Zihao;Ren, Jiali;Yang, Tao* ;Lin, Qinlu* ;...
期刊：
Sensors and Actuators B-Chemical ,2020年304:127283 ISSN：0925-4005
通讯作者：
Yang, Tao;Lin, Qinlu;Liu, Juewen
作者机构：
[Chen, Zihao; Yang, Tao; Lin, Qinlu; Ren, Jiali; Li, Wang] Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;[Li, Wang] Hunan Univ, State Key Lab Chemobiosensing & Chemometr, Changsha 410082, Hunan, Peoples R China.;[Yang, Tao; Lin, Qinlu] Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;[Liu, Juewen] Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G1, Canada.;[Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Waterloo, ON N2L 3G1, Canada.
通讯机构：
[Yang, T; Lin, QL] C;[Liu, Juewen] U;Cent South Univ Forestry & Technol, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G1, Canada.
关键词：
Nanoceria;Cr(3+)detection;Electric resistant;Multimeter;3D printed portable device
摘要：
Using simple and commonly available devices for signal reading has attracted much attention recently for DNA-based sensors. Most previous works focused on electrochemical or colorimetric signals. In this work, a portable device was structured by simple electronic and 3D-printed components to convert fluorescence signal to electric resistance with reading by a multimeter. To demonstrate its application, we took advantage of the slow ligand bind kinetics of Cr3+ to promote DNA adsorption on metal oxide nanoparticles (MONPs). Nanoceria (CeO2), screened from a total of 13 kinds of MONPs, possesses high specificity for Cr3+ from other metal ions. EDTA can mask other metal ions, while it cannot bind Cr3+ quickly so that Cr3+ promotes DNA adsorption and thus fluorescence quenching. The linear range is from 0 to 2 mu M Cr3+ with a LOD of 0.02 mu M. Using common electronic components and the 3D-printed miniaturized device, the sensor to transfer fluorescence to electric resistant signal was also measured by a digital multimeter. In this case, the LOD is about 1 mu M Cr3+. Such a strategy may provide a simple and general approach for fluorescent portable device fabrication and may find applications for other onsite sensing applications.
语种：
英文
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基于“食品营养与卫生学”课程思政教育的改革策略研究
作者：
邢艳霞;祝贺;常桂芳;田莹莹;杨涛
期刊：
农产品加工 ,2020年(1):108-109,112 ISSN：1671-9646
作者机构：
山东农业工程学院食品科学与工程学院,山东济南 250100;中南林业科技大学稻谷及副产物深加工国家工程实验室,湖南长沙 410000;[田莹莹; 祝贺; 常桂芳; 邢艳霞] 山东农业工程学院;[杨涛] 中南林业科技大学
关键词：
课程思政;道德素质;食品营养与卫生学;教育改革
摘要：
在多元价值体系逐渐渗透、交织的现状下,在高校开展课程思政教育是提升食品专业学生道德素质的有效途径之一。基于食品专业学生的教学特点,初步探究了"食品营养与卫生学"课程思政教学的策略及方法。
语种：
中文
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Protic Ionic Liquids as Efficient Solvents in Microwave-Assisted Extraction of Rhein and Emodin from Rheum palmatum L
作者：
Fan, Yunchang* ;Niu, Zeyu;Xu, Chen;Yang, Lei;Yang, Tuojie
期刊：
Molecules ,2019年24(15):2770 ISSN：1420-3049
通讯作者：
Fan, Yunchang
作者机构：
[Yang, Lei; Yang, Tuojie; Xu, Chen; Fan, Yunchang] Henan Polytech Univ, Coll Chem & Chem Engn, Jiaozuo 454003, Henan, Peoples R China.;[Niu, Zeyu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Fan, Yunchang] H;Henan Polytech Univ, Coll Chem & Chem Engn, Jiaozuo 454003, Henan, Peoples R China.
关键词：
anthraquinone derivative;emodin;imidazole derivative;ionic liquid;mesylic acid derivative;plant extract;rhein;solvent;chemistry;factor analysis;high performance liquid chromatography;human;isolation and purification;liquid liquid extraction;microwave radiation;procedures;Rheum;Anthraquinones;Chromatography, High Pressure Liquid;Emodin;Factor Analysis, Statistical;Humans;Imidazoles;Ionic Liquids;Liquid-Liquid Extraction;Mesylates;Microwaves;Plant Extracts;Rheum;Solvents
摘要：
Rheum palmatum L. (R. palmatum L.) is a traditional Chinese herb and food, in which rhein and emodin are the main bioactive components. The extraction of the two compounds from R. palmatum L. is, thus, of great importance. In this work, protic ionic liquids (PILs) were applied in the microwave-assisted extraction (MAE) of rhein and emodin from R. palmatum L., which avoids the toxicity of organic solvents. The results of the present study indicate that PILs possessing higher polarity exhibit higher extraction ability due to their stronger absorption ability for microwave irradiation. Compared with conventional solvents, such as methanol, trichloromethane, and deep eutectic solvents (DESs), the PIL, 1-butyl-3-himidazolium methanesulfonate ([BHim]MeSO3) reported herein is more efficient. The selected extraction conditions of liquid-solid ratio, microwave irradiation time, microwave irradiation power, and PIL concentration were 40 g·g-1, 50 s, 280 W, and 80%, respectively. Under the selected conditions, the extraction yields of rhein and emodin were 7.8 and 4.0 mg·g-1, respectively. These results suggest that PILs are efficient extraction solvents for the separation of active components from natural products.
语种：
英文
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响应面法优化纯种根霉米粉种曲制作工艺
作者：
吴健;祝贺;蓝彩红;刘盛钢;贺燕波;...
期刊：
中国酿造 ,2019年38(9):86-90 ISSN：0254-5071
作者机构：
中南林业科技大学 食品科学与工程学院,湖南 长沙,410000;中南林业科技大学 食品科学与工程学院,湖南 长沙 410000;山东农业工程学院食品科学与工程学院,山东 济南 250100;华泽集团有限公司,云南 迪庆,674400;华泽集团有限公司,云南 迪庆 674400
关键词：
根霉;米粉;试饭糖分;种曲;制作工艺;优化
摘要：
针对纯种根霉（Rhizopus）米粉种曲制备过程中缺乏系统控制、质量难以稳定的问题,研究其关键影响因素,优化种曲的制备工艺。以一级籼米米粉为原料,接种纯种根霉制备种曲,在单因素试验的基础上,以种曲的试饭糖分值为响应指标,利用Box-Behnken响应面设计试验优化种曲制作工艺,确定纯种根霉米粉种曲的最佳制备工艺为试管菌种培养时间72 h、二级种曲培养温度32℃、米粉水分含量25%。在此最佳工艺条件下进行验证试验,实际测得种曲的试饭糖分平均值为27.18 g/100 g,与模型的理论预测值非常接近,拟合成功;该模型优化工艺可为纯种根霉曲的生产提供参考。
语种：
中文
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Biomimetic mineralization of the carbonates regulated by using hydroxypropyl methylcellulose macromolecules as organic templates
作者：
Yang, Tianli;He, Ren;Omeoga, Uche;Wang, Lin;Sun, Renrui;...
期刊：
Journal of Crystal Growth ,2019年508:72-81 ISSN：0022-0248
通讯作者：
Wang, Wenlei
作者机构：
[Wang, Wenlei; Omeoga, Uche; Yang, Tianli; He, Ren; Zhang, Ning] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Sun, Renrui; Wang, Lin] Cent South Univ Forestry & Technol, Sch Mat Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Xu, Dong] Cent South Univ Forestry & Technol, Sch Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Zeng, Dewen] Cent S Univ, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China.
通讯机构：
[Wang, Wenlei] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.
关键词：
Binding energy;Biomimetics;Calcite;Calcium carbonate;Carbonate minerals;Carbonates;Carbonation;Mineralogy;Morphology;Polymorphism;Biomimetic mineralization;Crystal growth mechanism;HPMC;Hydroxypropyl methylcellulose;Magnesium concentrations;Mineralization process;Molar ratio;Polarizing microscopes;Magnesium compounds
摘要：
Control of the morphology of carbonate crystals plays an indispensable role in mineralization process. We investigated the relationship between HPMC concentration and different molar ratios of Mg/Ca on the carbonate crystallization by monitoring crystal polymorph, crystal size, and morphology. In this experiment, the total molars of [Mg 2+ ] and [Ca 2+ ] were 50 mM. The results demonstrated that different molar ratio of Ma/Ca primarily can help different morphology form in multidimensional spline space, for instance, lens-like calcite, spindle shaped aragonite and polyhedron nesquehonite. Moreover, the metastable aragonite phase of CaCO 3 can come into being with the existence of HPMC. Meanwhile, the Mg 2+ would inhibit the growth of calcite and facilitate the production of the aragonite when the Mg 2+ concentration is lower than 25 mM in solution. With increasing the molar ratio of Mg/Ca, the nesquehonite precipitation would be induced when the magnesium concentration is sufficiently high. The nesquehonite grew along the same (4 0 0) orientation in that the HPMC can be controlled by the basic units during the aggregation and reorientation process. The results of polarizing microscope also provided forceful support for the crystal growth mechanism. The thermodynamical stable phase of anhydrous calcium carbonate (CaCO 3 ) was nucleated preferentially after the combination between Ca 2+ and CO 3 2− , then, the Mg 2+ can bond with the oxygen atoms in HPMC due to electrostatic adherence and combine with CO 3 2− and H 2 O to form a nesquehonite crystal phase. The results illustrated that the extent of binding affinity during HPMC with Ca 2+ or Mg 2+ was suggested to be in connection with the reconstructuring process and the morphology of final carbonate crystals. © 2018
语种：
英文
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正交试验优化酿酒高粱振荡蒸煮工艺
作者：
祝贺;杨涛;杨玉蓉;郝哲兵;姚鹏;...
期刊：
中国酿造 ,2019年38(9):91-95 ISSN：0254-5071
作者机构：
中南林业科技大学 稻谷及副产物深加工国家工程实验室,湖南 长沙 410000;中南林业科技大学 食品科学与工程学院,湖南 长沙 410000;华泽集团有限公司,云南 迪庆 674400;华泽集团有限公司,云南 迪庆,674400;[蓝彩红; 吴健; 祝贺; 杨涛; 刘盛钢; 杨玉蓉] 中南林业科技大学
关键词：
高粱;振荡蒸煮工艺;正交试验;优化;感官评分;糊化焓值;酚类
摘要：
该研究在单因素试验的基础上,以蒸煮后高粱的感官评分、糊化焓值及总酚含量为评价指标,采用正交试验对高粱振荡蒸煮工艺进行优化.结果表明,最佳振荡蒸煮工艺为蒸煮温度90℃,蒸煮时间60 min,振荡频率100 r/min.采用最优振荡蒸煮工艺对高粱处理后,感官评分为16.21分,糊化焓值为13.47 J/g,总酚含量为0.44 mg/g,与传统工艺的感官评分(16.37分)、糊化焓值(13.92 J/g)、总酚含量(0.41 mg/g)无显著差异(P>0.05).振荡蒸煮工艺保留总黄酮类(0.22 mg/g)效果显著高于传统蒸煮工艺(0.13 mg/g)(P<0.05),保留原花色素(0.12 mg/g)能力显著低于传统工艺(0.19 mg/g)(P<0.05),与传统蒸煮工艺处理后的酚类组成有显著差异(P<0.05).
语种：
中文
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课程思政教育的改革探究——以《食品安全与卫生学》为例
作者：
祝贺;常桂芳;邢艳霞;田莹莹;杨涛
期刊：
教育现代化 ,2019年(88):182-183 ISSN：2095-8420
作者机构：
1. 山东农业工程学院食品科学与工程学院;2. 中南林业科技大学食品科学与工程学院
关键词：
课程思政;道德素质;食品安全与卫生学
摘要：
在多元价值体系逐渐渗透、交织的现状下,在高校开展课程思政教育是提升食品专业学生道德素质的有效途径之一。本文基于食品专业学生的教学特点,初步探究了《食品安全与卫生学》"课程思政"教学的策略及方法。
语种：
中文
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葡萄酒中酚类物质的研究进展
作者：
回学宽;王艳丽;祝贺;杨涛;邢艳霞
期刊：
中国果菜 ,2019年39(11):65-71+79 ISSN：1008-1038
作者机构：
山东农业工程学院食品科学与工程学院;[王艳丽; 邢艳霞] 中南林业科技大学;[祝贺; 杨涛; 回学宽] 山东农业工程学院
关键词：
葡萄酒;酚类物质;品质;功能
摘要：
酚类物质是葡萄酒中重要的品质贡献及功能性物质,一直以来都是葡萄酒研究的重点。酚类物质不仅可以影响葡萄酒的气味、颜色、口感等品质指标,且具有抗氧化的功能。但并非所有酚类物质对葡萄酒品质或功能具有积极作用,部分酚类物质可能会对葡萄酒品质或功能会产生负面影响。本文对葡萄酒中的酚类物质来源、化学组成等进行了综述,为研究葡萄酒酚类物质的品质、功能贡献及葡萄酒酚类的组成控制提供思路。
语种：
中文
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Preservation mechanism of high concentration carbon dioxide controlled atmosphere for paddy rice storage based on quality analyses and molecular modeling tools
作者：
Sun, Shuguo;Li, Beiping;Yang, Tao;Luo, Feijun;Zhao, Juanhong;...
期刊：
Journal of Cereal Science ,2019年85:279-285 ISSN：0733-5210
通讯作者：
Lin, Qinlu
作者机构：
[Luo, Feijun; Yang, Tao; Zhao, Juanhong; Cao, Jianzhong; Li, Beiping; Sun, Shuguo; Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Natl Engn Lab Deep Proc Rice & Byprod, Changsha 410004, Hunan, Peoples R China.;[Lin, Qinlu] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Lin, Qinlu] C;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.
关键词：
Controlled atmosphere storage;Mechanism;Molecular modeling tools;Paddy rice
摘要：
Use of carbon dioxide controlled atmospheres has proven to be effective for the preservation of cereal grain during storage, but its preservation mechanisms remain to be fully elucidated. In this study, preservation mechanism of high concentration carbon dioxide controlled atmosphere for paddy rice storage was evaluated based on quality analyses and a novel method using molecular modeling tools. During 90 days storage, the fat acidity, cooked rice hardness, and lipoxygenase activity of paddy rice were gradually increased and alpha-amylase activity was decreased rapidly, but the degree of change of the paddy rice stored in controlled atmosphere was lower than that in non-controlled atmosphere. The rate constants for hydration water disaggregation from biological macromolecules, such as proteins and starch in paddy rice, and isothermal kinetic parameters were calculated according to simple first-order kinetics and the Arrhenius's law. The hydration water of paddy rice stored in non-controlled atmosphere presented the higher rate constant for degradation, especially at higher temperatures. Values of activation energies were 28.27 and 15.80 kJ/mol, respectively, for hydration water of paddy rice stored in controlled and non-controlled atmosphere. The mechanism of controlled atmospheres for paddy rice preservation may be the higher of activation energies for hydration water or biological macromolecules. © 2018
语种：
英文
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酚类对酒的风味影响及白酒酚类控制策略
作者：
祝贺;杨涛;郝哲兵;姚鹏;吴健;...
期刊：
食品工业科技 ,2019年40(18):361-367 ISSN：1002-0306
作者机构：
[蓝彩红; 吴小霞; 吴健; 祝贺; 杨涛; 刘盛钢] 中南林业科技大学;中南林业科技大学食品科学与工程学院;华泽集团有限公司;[郝哲兵; 姚鹏] 华泽集团
关键词：
酚类;含量;组成;白酒风味品质
摘要：
酚类物质是存在于大部分酒中的风味物质,对酒风味品质控制具有重要意义。酒体中的酚类物质组成及含量存在很大区别,本文对酒中酚类物质的来源、含量、化学组成对酒品质的影响及控制策略进行综述,为研究白酒酚类差异的产生原因及控制方法提供思路。
语种：
中文
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δ-Tocotrienol, Isolated from Rice Bran, Exerts an Anti-Inflammatory Effect via MAPKs and PPARs Signaling Pathways in Lipopolysaccharide-Stimulated Macrophages
作者：
Shen, Junjun;Yang, Tao;Xu, Youzhi;Luo, Yi;Zhong, Xinyue;...
期刊：
International Journal of Molecular Sciences ,2018年19(10):3022 ISSN：1661-6596
通讯作者：
Luo, Feijun;Lin, Qinlu
作者机构：
[Hu, Tao; Luo, Feijun; Yang, Tao; Guo, Tianyi; Zhong, Xinyue; Shi, Limin; Luo, FJ; Lin, Qinlu; Shen, Junjun; Nie, Ying; Xu, Youzhi] Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha 410004, Hunan, Peoples R China.;[Luo, Yi] Cent S Univ, Xiangya Sch Med, Dept Clin Med, Changsha 410008, Hunan, Peoples R China.
通讯机构：
[Luo, FJ; Lin, QL] C;Cent South Univ Forestry & Technol, Hunan Key Lab Proc Food Special Med Purpose, Hunan Key Lab Grain Oil Deep Proc & Qual Control, Coll Food Sci & Engn,Natl Engn Lab Deep Proc Rice, Changsha 410004, Hunan, Peoples R China.
关键词：
alpha tocopherol;antiinflammatory agent;cytokine;immunoglobulin enhancer binding protein;lipopolysaccharide;mitogen activated protein kinase 1;mitogen activated protein kinase 3;mitogen activated protein kinase kinase 4;nitric oxide;peroxisome proliferator activated receptor;tocotrienol, delta;analogs and derivatives;animal;cell line;chemistry;drug effect;macrophage;MAPK signaling;metabolism;mouse;Oryza;Animals;Anti-Inflammatory Agents;Cell Line;Cytokines;Lipopolysaccharides;Macrophages;MAP Kinase Kinase 4;MAP Kinase Signaling System;Mice;Mitogen-Activated Protein Kinase 1;Mitogen-Activated Protein Kinase 3;NF-kappa B;Nitric Oxide;Oryza;Peroxisome Proliferator-Activated Receptors;Vitamin E
摘要：
δ-Tocotrienol, an important component of vitamin E, has been reported to possess some physiological functions, such as anticancer and anti-inflammation, however their molecular mechanisms are not clear. In this study, δ-tocotrienol was isolated and purified from rice bran. The anti-inflammatory effect and mechanism of δ-tocotrienol against lipopolysaccharides (LPS) activated pro-inflammatory mediator expressions in RAW264.7 cells were investigated. Results showed that δ-tocotrienol significantly inhibited LPS-stimulated nitric oxide (NO) and proinflammatory cytokine (TNF-α, IFN-γ, IL-1β and IL-6) production and blocked the phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular regulated protein kinases 1/2 (ERK1/2). δ-Tocotrienol repressed the transcriptional activations and translocations of nuclear factor-kappa B (NF-κB) and activator protein-1 (AP-1), which were closely related with downregulated cytokine expressions. Meanwhile, δ-tocotrienol also affected the PPAR signal pathway and exerted an anti-inflammatory effect. Taken together, our data showed that δ-tocotrienol inhibited inflammation via mitogen-activated protein kinase (MAPK) and peroxisome proliferator-activated receptor (PPAR) signalings in LPS-stimulated macrophages. © 2018 by the authors. Licensee MDPI, Basel, Switzerland.
语种：
英文
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Preparation and Evaluation of Smart Nanocarrier Systems for Drug Delivery Using Magnetic Nanoparticle and Avidin-Iminobiotin System
作者：
Sun, Shuguo;Li, Beiping;Yang, Tao* ;Ma, Meihu* ;Lin, Qinlu;...
期刊：
Journal of Nanomaterials ,2018年2018:1-11 ISSN：1687-4110
通讯作者：
Yang, Tao;Ma, Meihu
作者机构：
[Luo, Feijun; Yang, Tao; Zhao, Juanhong; Li, Beiping; Sun, Shuguo; Lin, Qinlu] Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Proc, Changsha 410004, Hunan, Peoples R China.;[Ma, Meihu; Sun, Shuguo] Huazhong Agr Univ, Natl R&D Ctr Egg Proc, Wuhan 430070, Hubei, Peoples R China.;[Luo, Feijun] Univ Cambridge, Addenbrookes Hosp, Dept Pathol, Cambridge, England.
通讯机构：
[Yang, Tao] C;[Ma, Meihu] H;Cent South Univ Forestry & Technol, Natl Engn Lab Rice & Byprod Proc, Changsha 410004, Hunan, Peoples R China.;Huazhong Agr Univ, Natl R&D Ctr Egg Proc, Wuhan 430070, Hubei, Peoples R China.
摘要：
<jats:p>Therapeutic efficacy and the regulation of drug release can be improved by using selective targeting drug delivery systems. In this paper, we have demonstrated avidin-immobilized magnetic nanoparticles (AMNPs) as a novel targeted drug delivery system to deliver iminobiotinylated daunomycin (IDAU). TEM, XRD, VSM, and FTIR were employed for the physicochemical characterization of the drug-loaded MNPs. The binding of IDAU had little effect on sizes of AMNPs (~35 nm), but the stability and dispersibility of the nanoparticles were improved. The study also found that the loading capacity and efficiency of nanoparticles were mainly dependent on affinity interaction between IDAU and AMNPs. The optimal loading capacity and efficiency of MNPs for IDAU were 0.408 ± 0.012 mg/g and 94.18 ± 2.64% according to the reversed-phase high-performance liquid chromatography (RP-HPLC) data, respectively. Under the conditions of pH 6.8 and 1 mmol/L of biotin, the drug-loaded MNPs released rapidly at beginning and then maintained at a certain controllable release level. The effect of IDAU on DLKP proliferation was tested, and the results showed that IC50 was (1.60 ± 0.05) × 10<jats:sup>−3</jats:sup> mg/mL. Our findings indicated that AMNPs hold tremendous potential as an effective drug delivery system.</jats:p>
语种：
英文
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陶瓷容器贮酒与酒体品质的关联性研究
作者：
黄慧芬;刘俊;吴小霞;吴健;杨涛
期刊：
酿酒科技 ,2018年(6):65-71 ISSN：1001-9286
作者机构：
中南林业科技大学, 食品科学与工程学院, 稻谷及副产物深加工国家工程实验室, 湖南 长沙 410000;[黄慧芬; 吴小霞; 吴健; 杨涛; 刘俊] 中南林业科技大学
关键词：
贮存容器;酯类物质;感官分析;贮存环境
摘要：
经发酵、蒸馏而得的新酒,必须经过一段时间的贮存,以保证白酒风味品质。白酒贮存容器种类很多,有陶瓷容器、不锈钢容器及铺有陶瓷板的地下酒池,不同的贮酒容器对白酒品质影响不同。相对于不锈钢酒罐和地下池贮酒,陶瓷容器贮存的酒样中主要酯类物质变化较快,新酒气味明显减弱,硫化物等异味物质减轻,燥辣感减弱,口感变得醇和,甜度略有增强。外部环境条件选择对酒质的优劣也至关重要,露天式贮存环境下白酒酒样质量损耗大,地窖封闭式环境贮存酒样口感更稳定,酒体更加丰满。
语种：
中文
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A DNA as a Substrate and an Enzyme: Direct Profiling of Methyltransferase Activity by Cytosine Methylation of a DNAzyme
作者：
Li, Wang;Chen, Siyi;Xu, Dong;Wen, Qian;Yang, Tao* ;...
期刊：
CHEMISTRY-A EUROPEAN JOURNAL ,2018年24(54):14500-14505 ISSN：0947-6539
通讯作者：
Yang, Tao;Liu, Juewen
作者机构：
[Chen, Siyi; Yang, Tao; Li, Wang; Xu, Dong; Wen, Qian] Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;[Li, Wang; Liu, Juewen] Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G7, Canada.;[Li, Wang; Liu, Juewen] Univ Waterloo, Waterloo Inst Nanotechnol, Waterloo, ON N2L 3G7, Canada.
通讯机构：
[Yang, Tao] C;[Liu, Juewen] U;Cent South Univ Forestry & Technol, Coll Food Sci & Engn, Hunan Key Lab Proc Food Special Med Purpose, Changsha 410004, Hunan, Peoples R China.;Univ Waterloo, Dept Chem, Water Inst, Waterloo, ON N2L 3G7, Canada.;Univ Waterloo, Waterloo Inst Nanotechnol, Waterloo, ON N2L 3G7, Canada.
关键词：
aptamers;biosensors;cancer;DNAzymes;inhibitors
摘要：
Inhibiting DNA methyltransferase (MTase) activity is crucial for cancer treatment. Evaluating drug candidates as inhibitors of MTase requires the measurement of its activity. However, direct profiling of MTase activity remains an analytical challenge, since a complicated hydrolysis step using methylation-sensitive restriction enzymes (msRE) was inevitable in almost all previously reported methods. Taking advantage that DNA is the substrate of MTase and certain DNA sequences, known as DNAzymes, can also have enzyme-like activities, we herein developed an enzyme-free and label-free route for direct assaying MTase activity. Specifically, adding a cytosine at the meta-position of a peroxidase-mimicking DNAzyme can improve the catalytic activity of DNAzyme up to 5-fold. After methylation of the cytosine cap, the activity is further doubled. Based on these findings, direct assaying of MTase inhibitors was performed without the extra and complicated hydrolysis step. These new findings provide a helpful tool for screening drugs for cancer therapy. The idea of using the same DNA as a substrate and an enzyme could be a general way for assaying other enzymes. © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
语种：
英文
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