Digital image colorimetry on smartphone for chemical analysis: A review
作者：
Fan, Yajun;Li, Jiawei;Guo, Yaping;Xie, Lianwu* ;Zhang, Gui*
期刊：
Measurement ,2021年171:108829 ISSN：0263-2241
通讯作者：
Xie, Lianwu;Zhang, Gui
作者机构：
[Fan, Yajun; Zhang, Gui; Li, Jiawei; Xie, Lianwu; Guo, Yaping] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.;[Zhang, Gui] Cent South Univ Forestry & Technol, Key Lab Digital Dongting Lake Basin Hunan Prov, Changsha 410004, Peoples R China.
通讯机构：
[Xie, LW; Zhang, G] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.
关键词：
Cameras;Color;Colorimeters;Image analysis;Lighting;Medical imaging;Metal analysis;mHealth;Optimization;Smartphones;Analytical procedure;Basic procedure;Camera technology;Continuous development;Continuous optimization;Current status;Lighting conditions;Natural compounds;Colorimetry
摘要：
Digital image colorimetry (DIC) on smartphone is regarded as a powerful, fast and low-cost analysis method to measure target analyte with color changes of digital image obtained by the built-in camera. We summarized the basic procedure of DIC, the color spaces (RGB, CMYK, HSB/HSL, CIE XYZ, L*a*b*, and YUV), the principal architectures (tools for capturing image, lighting conditions, and color quantification APPs and DIC APPs), and current status of DIC on smartphone in analysis of metals/heavy metals, herbicides, pesticides, antibiotics, biological and medical indicators, natural compounds, and bacteria/viruses. The advantages and disadvantages of DIC are also revealed. Nowadays, DIC on smartphone must be further refined with controlled geometry and standard lighting sources to become robust and reliable analytical procedures. And it will be improved in the near future with the continuous development of smartphones owing to the rapid development of smartphone's camera technology and the continuous optimization of related software. © 2020 Elsevier Ltd
语种：
英文
展开
Rapid and comprehensive profiling of α-glucosidase inhibitors in Buddleja Flos by ultrafiltration HPLC–QTOF-MS/MS with diagnostic ions filtering strategy
作者：
Xie, Lianwu* ;Fu, Qiachi;Shi, Shuyun* ;Li, Jiawei;Zhou, Xinji
期刊：
Food Chemistry ,2021年344:128651 ISSN：0308-8146
通讯作者：
Xie, Lianwu;Shi, Shuyun
作者机构：
[Shi, Shuyun; Xie, Lianwu; Shi, SY; Li, Jiawei; Zhou, Xinji] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.;[Shi, Shuyun; Fu, Qiachi] Cent South Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China.
通讯机构：
[Xie, LW; Shi, SY] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Peoples R China.
关键词：
Diagnostic ions filtering;Flavonoid;HPLC–QTOF-MS/MS;Phenylethanoid glycoside;Ultrafiltration;α-Glucosidase inhibitor
摘要：
Buddleja Flos is used as yellow rice colorant and a well-known traditional Chinese medicine. But its biochemical profiling is still lack due to complex matrix. Here, ultrafiltration high-performance liquid chromatograph–quadrupole time-of-flight tandem mass spectrometry (HPLC–QTOF-MS/MS) with diagnostic ions filtering strategy was proposed for rapid and comprehensive investigation of its α-glucosidase inhibitors. As a result, 33 bioactive compounds (13 phenylethanoid glycosides and 20 flavonoids) were successfully screened and identified. In addition, α-glucosidase inhibitory activities of twenty-two references were verified. Six flavonoid aglycones (4, 28, and 30–33) showed excellent α-glucosidase inhibitory activities (IC50, from 5.11 ± 0.85 to 32.49 ± 9.76 μg/mL), much higher than that of acarbose (IC50, 195.49 ± 10.05 μg/mL). Five flavonoid-monoglycosides (7, 12, 13, 20, and 22) presented moderate inhibitory activities with IC50 from 160.98 ± 23.19 to 249.37 ± 35.83 μg/mL. Results showcased the high efficiency of proposed strategy in profiling of bioactive compounds from natural products. © 2020 Elsevier Ltd
语种：
英文
展开
智能手机数字图片比色分析法概述
作者：
范亚军;李嘉伟;郭亚平;谢练武
期刊：
食品安全质量检测学报 ,2020年11(5):1651-1658 ISSN：2095-0381
作者机构：
中南林业科技大学理学院,长沙 410004;[谢练武; 范亚军; 郭亚平; 李嘉伟] 中南林业科技大学
关键词：
比色分析;智能手机;数字图片;颜色模型;图像传感器;食品安全;快速检测
摘要：
为保护环境与食品安全,有毒有害物质的现场快速检测以及分析数据即时反馈显得越发重要。随着智能手机的广泛普及与其应用程序的不断完善,智能手机数字图片比色分析法得到了快速发展。本文简述了智能手机数字图片比色法的基本原理、图片采集设备及其传感器类型、图片颜色模型、图片数字化软件、分析数据处理与网络共享,以及该方法在食品安全、医疗诊断、生物检测等领域的研究与应用,对数字图片比色分析法未来的发展也做出了展望。得益于智能手机软硬件的不断完善,数字图片比色分析法将得到广泛应用。
语种：
中文
展开
Vinyl Phosphate-Functionalized, Magnetic, Molecularly-Imprinted Polymeric Microspheres' Enrichment and Carbon Dots' Fluorescence-Detection of Organophosphorus Pesticide Residues
作者：
Wu, Mao;Fan, Yajun;Li, Jiawei;Lu, Danqing;Guo, Yaping;...
期刊：
Polymers ,2019年11(11):1770 ISSN：2073-4360
通讯作者：
Xie, Lianwu;Wu, Yiqiang
作者机构：
[Fan, Yajun; Wu, Mao; Lu, Danqing; Li, Jiawei; Xie, Lianwu; Guo, Yaping] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Wu, Yiqiang] Cent South Univ Forestry & Technol, Coll Mat Sci & Technol, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Xie, Lianwu; Wu, Yiqiang] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Mat Sci & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
fluorescence detection;magnetic molecularly imprinted polymeric microspheres;organophosphorus pesticides;pesticide residues;sensor
摘要：
The rapid detection of organophosphorus pesticide residues in food is crucial to food safety. One type of novel, magnetic, molecularly-imprinted polymeric microsphere (MMIP) was prepared with vinyl phosphate and 1-octadecene as a collection of dual functional monomers, which were screened by Gaussian09W molecular simulation. MMIPs were used to enrich organic phosphorus, which then detected by fluorescence quenching in vinyl phosphate-modified carbon dots ([email protected]) originated from anhydrous citric acid. MMIPs and [email protected] were characterized by TEM, particle size analysis, FT-IR, VSM, XPS, adsorption experiments, and fluorescence spectrophotometry in turn. Through the fitting data from experiment and Gaussian quantum chemical calculations, the molecular recognition properties and the mechanism of fluorescence detection between organophosphorus pesticides and [email protected] were also investigated. The results indicated that the MMIPs could specifically recognize and enrich triazophos with the saturated adsorption capacity 0.226 mmol g-1, the imprinting factor 4.59, and the limit of recognition as low as 0.0006 mmol L-1. Under optimal conditions, the [email protected] sensor has shown an extensive fluorescence property with a LOD of 0.0015 mmol L-1 and the linear range from 0.0035 mmol L-1 to 0.20 mmol L-1 (R2 = 0.9988) at 390 nm. The mechanism of fluorescence detection of organic phosphorus with [email protected] sensor might be attributable to hydrogen bonds formed between heteroatom O, N, S, or P, and the O-H group, which led to fluorescent quenching. Meanwhile, HN-C=O and Si-O groups in [email protected] system might contribute to cause excellent blue photoluminescence. The fluorescence sensor was thorough successfully employed to the detection of triazophos in cucumber samples, illustrating its tremendous value towards food sample analysis in complex matrix. © 2019 by the authors.
语种：
英文
展开
Cetyltrimethyl ammonium mediated enhancement of the red emission of carbon dots and an advanced method for fluorometric determination of iron(III)
作者：
Li, Te;Xie, Lianwu;Long, Ruiqing;Tong, Chaoying;Guo, Ying;...
期刊：
Microchimica Acta ,2019年186(12):791 ISSN：0026-3672
通讯作者：
Shi, Shuyun;Lin, Qinlu
作者机构：
[Shi, Shuyun; Tong, Chaoying; Tong, Xia; Li, Te; Long, Ruiqing] Cent S Univ, Coll Chem & Chem Engn, Key Lab Hunan Prov Water Environm & Agr Prod Safe, Changsha 410083, Hunan, Peoples R China.;[Shi, Shuyun; Shi, SY; Lin, Qinlu; Xie, Lianwu] Cent South Univ Forestry & Technol, Coll Sci, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;[Shi, Shuyun; Guo, Ying] Cent S Univ, Xiangya Hosp, Dept Clin Pharmacol, Hunan Key Lab Pharmacogenet, Changsha 410078, Hunan, Peoples R China.
通讯机构：
[Shi, Shuyun; Shi, SY; Lin, QL] C;Cent S Univ, Coll Chem & Chem Engn, Key Lab Hunan Prov Water Environm & Agr Prod Safe, Changsha 410083, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Sci, Coll Food Sci & Engn, Changsha 410004, Hunan, Peoples R China.;Cent S Univ, Xiangya Hosp, Dept Clin Pharmacol, Hunan Key Lab Pharmacogenet, Changsha 410078, Hunan, Peoples R China.
关键词：
Fluorometric assay;Human plasma;Metal ion;Nanomaterial;Quenching mechanism;Surface functionalization;Surfactant
摘要：
Red-emissive carbon dots (CDs) were synthesized by one-step hydrothermal technique using citric acid (CA), and urea in N,N-dimethylformamide (DMF) solution. The CDs has an average diameter of 2.3 nm, excitation/emission maxima at 553/606 nm, and a low photoluminescence quantum yield (4%). Fluorescence is weakly quenched by the ions Fe(3+), Hg(2+), Cu(2+), Co(2+), Zn(2+), Ca(2+), Ni(2+), and Pb(2+). After addition of cetyltrimethyl ammonium ion (CTAB), electrostatic interaction between negatively charged CDs and CTAB causes the CDs to self-aggregate. The formation of CD/CTAB increases the average particle diameter to around 13 nm and enhances the quantum yield to 24%. The hydrophobic segments of CTAB twined into a network structure can selectively trap Fe(3+) and then interact with surface groups of the CDs to cause quenching. The CD/CTAB nanoprobe enables fluorometric determination of Fe(3+) with a linear response in the 0.10-10 muM concentration range and a 0.03 muM limit of detection. The probe was utilized for determination of Fe(3+) in human serum samples, and satisfactory results were obtained. Graphical abstractSchematic representation of fluorometric analysis of Fe(III) ion by cetyltrimethyl ammonium ion (CTAB) mediated red emission carbon dots (CDs). The hydrophobic segments of CTAB twined into a network structure can selectively trap Fe(III) and then interact with surface groups of the CDs to cause quenching.
语种：
英文
展开
Rapidly colorimetric detection of caffeine in beverages by silver nanoparticle sensors coupled with magnetic molecularly imprinted polymeric microspheres
作者：
Deng, Huiyun;Wang, Bin;Wu, Mao;Deng, Bin;Xie, Lianwu* ;...
期刊：
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY ,2019年54(1):202-211 ISSN：0950-5423
通讯作者：
Xie, Lianwu;Guo, Yaping
作者机构：
[Deng, Huiyun; Wu, Mao; Xie, Lianwu; Wang, Bin; Guo, Yaping] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Deng, Bin] Xiangnan Univ, Coll Chem Biol & Environm Engn, Chenzhou 423043, Peoples R China.;[Xie, Lianwu] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Xie, LW; Guo, YP; Xie, Lianwu] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
Caffeine;colorimetric analysis;molecularly imprinted polymeric microspheres;silver nanoparticles
摘要：
Excessively intaking caffeine can harm human health, and it is imperative to develop a rapid method to identify caffeine for food safety. The magnetic molecularly imprinted polymeric microspheres (MMIPs) were prepared to pretreat samples before silver nanoparticles (AgNPs) colorimetric detection of caffeine. Using Fe3O4 as supporting core, mesoporous SiO2 as intermediate shell, α-methylacrylic acid as functional monomer and caffeine as template, the prepared MMIPs were characterised by transmission electron microscopy, Fourier transform infrared spectroscopy and vibrating sample magnetometer. Adsorption process followed Langmuir adsorption isotherm with maximum adsorption capacity at 38.8mgg−1 under 298K. The MMIPs extract caffeine from beverages, then the AgNPs colorimetric method rapidly screened and semi-quantified caffeine ≥5mgL−1 by naked eye, and accurately quantified caffeine ranging 0.1–5mgL−1 by UV-vis spectroscopy at 393nm, which were consistent with HPLC analytical results. Thus, this method might be used to rapidly enrich and analyse caffeine in beverages. © 2018 Institute of Food Science and Technology
语种：
英文
展开
以《无机及分析化学》为例浅议学生创新能力培养
作者：
郭鑫;袁寒梦;罗秋艳;肖滕;王文磊;...
期刊：
广州化工 ,2019年47(19):146-147,150 ISSN：1001-9677
作者机构：
中南林业科技大学理学院,湖南 长沙,410004;中南林业科技大学材料学院,湖南 长沙,410004;[谢练武; 肖滕; 罗秋艳; 袁寒梦; 王文磊; 郭鑫] 中南林业科技大学
关键词：
教学改革;翻转课堂;化学课程;创新能力
摘要：
针对当前高等林业院校化学课程教学方法单一的现状,以学生创新能力培养为导向,利用"翻转课堂"教学法、"问题式"教学法等多元教学法,进行 《无机及分析化学》 课程教学改革,构建"启发式"、"互动式"、"重基础"与"重创新"四位一体课程教学体系.通过招聘单位对应聘者的满意程度、 大大上升的深造比率、 毕业生对学校培养模式的满意程度、 显著提高的学生创新能力等方面,证明 《无机及分析化学》 课程教学改革方案确实可行.
语种：
中文
展开
基于翻转课堂的《无机及分析化学》教学改革
作者：
郭鑫;袁寒梦;罗秋艳;肖滕;谢练武;...
期刊：
广州化工 ,2019年47(20):144-145,160 ISSN：1001-9677
作者机构：
中南林业科技大学理学院,湖南 长沙,410004;中南林业科技大学材料学院,湖南 长沙,410004;[谢练武; 肖滕; 罗秋艳; 袁寒梦; 王文磊; 郭鑫] 中南林业科技大学
关键词：
翻转课堂;无机及分析化学;教学改革
摘要：
根据 《无机及分析化学》 课程的教学要求与学生的实际情况,以高等林业院校林产化工及相关专业无机及分析化学的教学情况为依据,采用新的教学模式对公共基础课无机及分析化学进行变革与探索.以 《无机及分析化学》 重要知识点"金属指示剂"一章为例,论述 《无机及分析化学》 教学课堂独特的教学模式及其优势,证实了翻转课堂教学模式能够显著提高学生课堂效率和加强学生科研创新和思辨能力.
语种：
中文
展开
没食子酸磁性表面分子印迹聚合物的制备与选择性识别性能
作者：
邓慧芸;王斌;吴茂;文瑞芝;马强;...
期刊：
应用化学 ,2018年35(5):600-608 ISSN：1000-0518
作者机构：
中南林业科技大学理学院,应用化学研究所, 长沙, 410004;湘南学院化学生物与环境工程学院, 湖南, 郴州, 423043;[邓慧芸; 王斌; 吴茂; 文瑞芝; 马强; 郭亚平; 谢练武] 中南林业科技大学理学院,应用化学研究所, 长沙, 410004;[邓斌] 湘南学院化学生物与环境工程学院, 湖南, 郴州, 423043
关键词：
表面分子印迹;选择性识别;羟基苯甲酸类化合物;吸附;磁性Fe_3O_4
摘要：
羟基苯甲酸类化合物用途广泛,极性较强,在复杂水溶液体系中这些类似物的分离纯化与分析非常困难.本文以磁性Fe_3O_4纳米颗粒为载体,没食子酸(GA)为模板分子,制备了磁性表面分子印迹聚合物(MIP).利用透射电子显微镜、红外光谱、磁强测定等检测手段对MIP进行了结构表征.并对其吸附性能进行研究,比较了该MIP对GA及其它3种结构类似物的吸附性能差异.结果表明,制备的以GA为模板的磁性分子印迹聚合物为核壳球形结构,键合牢固,对GA的吸附动力学符合准二级动力学方程模型,吸附过程属于Langmuir单分子层吸附.该聚合物对GA表现出优异的选择性识别能力,其吸附量(318 K时37.736 mg /g)远远高于结构类似物.该磁性分子印迹聚合物对模板分子不仅具有特异识别能力,而且能够磁控分离,分离效率高,可用于固相萃取.
语种：
中文
展开
有机磷农药残留分子印迹聚合物研究现状与展望
作者：
吴茂;范亚军;郭亚平;谢练武
期刊：
农药学学报 ,2018年20(6):689-703 ISSN：1008-7303
作者机构：
[吴茂; 范亚军; 郭亚平; 谢练武] 中南林业科技大学理学院, 长沙, 410004
关键词：
有机磷农药;残留;分子印迹聚合物;计算化学;传感器;仿生催化;样品前处理
摘要：
为解决有机磷农药在土壤及水体中的残留污染问题,分子印迹技术在该类农药残留检测中的应用研究已得到迅速发展。文章就有机磷农药分子印迹聚合物(molecularly imprinted polymers,MIPs)的研究背景、分子印迹技术基本原理及分类、计算化学在筛选优化虚拟模板及功能单体中的应用,以及有机磷农药残留MIPs的制备与应用研究等内容进行了综述。 MIPs因具有结构可预测、特异识别和应用普遍的特性,可广泛用于光学传感、化学传感、电化学传感、光电传感、质量敏感传感、仿生催化及样品前处理等众多领域。可通过Discovery Studio、HyperChem及Gaussian 3种分子模拟软件对(虚拟)模板、功能单体以及二者所形成的加合物进行计算模拟以获得最低能量的优势构象,通过计算加合物的结合能来优化筛选(虚拟)模板和功能单体。将计算化学与实验研究相结合,相信在未来几年,有机磷农药残留MIPs有可能在原位显色传感、农产品农药残留高通量筛查及计算机自动化检测等领域得到长足发展。
语种：
中文
展开
Magnetic surface molecularly imprinted polymeric microspheres using gallic acid as a segment template for excellent recognition of ester catechins
作者：
Wang, Bin;Deng, Huiyun;Wu, Mao;Xiang, Shu;Ma, Qiang;...
期刊：
ANALYTICAL METHODS ,2018年10(27):3317-3324 ISSN：1759-9660
通讯作者：
Xie, Lianwu;Guo, Yaping
作者机构：
[Ma, Qiang; Shi, Shuyun; Deng, Huiyun; Wu, Mao; Xiang, Shu; Xie, Lianwu; Wang, Bin; Guo, Yaping] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Shi, Shuyun; Xie, Lianwu] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Xie, LW; Guo, YP; Xie, Lianwu] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
摘要：
Magnetic surface molecularly imprinted polymeric microspheres (MMIPs) were prepared by using gallic acid (GA) as a segment template for the solid extraction of ester catechins as a whole from complex samples. MMIPs were characterized by Fourier transform infrared spectroscopy, transmission electron microscopy, and vibrating sample magnetometry. The results of the adsorption experiments indicated that the saturated adsorption capacity of GA on MMIPs was 0.21 mmol g-1, and the imprinting factor was 5.86; MMIPs had higher adsorption capacity for ester catechins than benzoic acid, salicylic acid, and 2,4-dihydroxybenzoic acid. The adsorption process of MMIPs could be better described by pseudo secondary rate equation, and it was consistent with the Langmuir adsorption model. In addition to GA, MMIPs could selectively recognize ester catechins in a tea solution without column packing. Thus, MMIPs prepared using GA as a segment template can be used in selective separation and enrichment of ester catechins from tea samples for functional food production. © 2018 The Royal Society of Chemistry.
语种：
英文
展开
Rapid colorimetric detection of cartap residues by AgNP sensor with magnetic molecularly imprinted microspheres as recognition elements
作者：
Wu, Mao;Deng, Huiyun;Fan, Yajun;Hu, Yunchu;Guo, Yaping;...
期刊：
Molecules ,2018年23(6):1443 ISSN：1420-3049
通讯作者：
Xie, Lianwu
作者机构：
[Fan, Yajun; Hu, Yunchu; Deng, Huiyun; Wu, Mao; Xie, Lianwu; Guo, Yaping] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;[Xie, Lianwu] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
通讯机构：
[Xie, Lianwu] C;Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
carbamothioic acid, S,S'-(2-(dimethylamino)-1,3-propanediyl) ester;magnetite;metal nanoparticle;microsphere;silicon dioxide;silver;thiocarbamic acid derivative;chemical structure;chemistry;colorimetry;molecular imprinting;procedures;tea;Colorimetry;Ferrosoferric Oxide;Metal Nanoparticles;Microspheres;Molecular Imprinting;Molecular Structure;Silicon Dioxide;Silver;Tea;Thiocarbamates
摘要：
The overuse of cartap in tea tree leads to hazardous residues threatening human health. A colorimetric determination was established to detect cartap residues in tea beverages by silver nanoparticles (AgNP) sensor with magnetic molecularly imprinted polymeric microspheres (Fe3O4@mSiO2@MIPs) as recognition elements. Using Fe3O4 as supporting core, mesoporous SiO2 as intermediate shell, methylacrylic acid as functional monomer, and cartap as template, Fe3O4@mSiO2@MIPs were prepared to selectively and magnetically separate cartap from tea solution before colorimetric determination by AgNP sensors. The core-shell Fe3O4@mSiO2@MIPs were also characterized by FT-IR, TEM, VSM, and experimental adsorption. The Fe3O4@mSiO2@MIPs could be rapidly separated by an external magnet in 10 s with good reusability (maintained 95.2% through 10 cycles). The adsorption process of cartap on Fe3O4@mSiO2@MIPs conformed to Langmuir adsorption isotherm with maximum adsorption capacity at 0.257 mmol/g and short equilibrium time of 30 min at 298 K. The AgNP colorimetric method semi-quantified cartap5 mg/L by naked eye and quantified cartap 0.1-5 mg/L with LOD 0.01 mg/L by UV-vis spectroscopy. The AgNP colorimetric detection after pretreatment with Fe3O4@mSiO2@MIPs could be successfully utilized to recognize and detect cartap residues in tea beverages. © 2018 by the authors.
语种：
英文
展开
安化黑茶水溶性成分高效液相色谱指纹图谱及定量分析
作者：
王斌;邓慧芸;吴茂;郭亚平;邓斌;...
期刊：
食品科学 ,2017年38(20):125-130 ISSN：1002-6630
通讯作者：
Xie, Lianwu(xiecsu@126.com)
作者机构：
[马强; 谢练武; 邓慧芸; 邓斌; 郭亚平; 王斌; 吴茂] Institute of Applied Chemistry, College of Sciences, Central South University of Forestry and Technology, Changsha, 410004, China;[邓斌] School of Chemical Biology and Environmental Engineering, Xiangnan University, Chenzhou, 423043, China
关键词：
安化黑茶;水溶性成分;指纹图谱;高效液相色谱;化学模式识别
摘要：
目的:运用高效液相色谱(high performance liquid chromatography,HPLC)法研究湖南安化黑茶指纹图谱,并对安化黑茶的主要成分进行定量分析,为湖南安化黑茶的成分分析与鉴别提供理论依据。方法:以22个安化黑茶样品与4种外省黑茶(云南普洱茶、湖北青砖茶、广西六堡茶、四川土司黑茶)为实验材料,采用梯度洗脱(A相为0.17%乙酸溶液,B相为乙腈),柱温30 ℃,检测波长280 nm,流速1.0 mL/min,建立安化黑茶HPLC指纹图谱,并进行了相似度评价与主成分分析,确定共有色谱峰。结果:22个安化黑茶样品具有19个共有色谱峰,这些黑茶样品在共有模式下的相似度为0.783~0.958,而广西六堡茶、四川土司黑茶、湖北青砖茶以及云南普洱茶与安化黑茶相似度很低(<0.3);定量分析表明,安化黑茶中没食子酸、表没食子儿茶素没食子酸酯的含量明显高于4种外省黑茶,而广西六堡茶中的咖啡因和云南普洱茶中的没食子儿茶素含量明显高于安化黑茶;此外,茯砖素A仅在安化黑茶中检出,可作为安化黑茶的标志性成分。结论:根据所构建的安化黑茶HPLC指纹图谱及找到的标志性成分茯砖素A,可用于安化黑茶的鉴定与评价。
语种：
中文
展开
林业高校化学学科“四层次一体化”研究生创新培养模式
作者：
谢练武;郭亚平;皮少锋;郭鑫;王文磊;...
期刊：
教育教学论坛 ,2017年(41):238-239 ISSN：1674-9324
作者机构：
中南林业科技大学 理学院,长沙,410004;[谢练武; 郭亚平; 黄自知; 王文磊; 郭鑫; 皮少锋] 中南林业科技大学
关键词：
研究生教育;四层次一体化;创新;培养模式
摘要：
为适应创新驱动发展新常态，我院在林业高校化学类研究生课程建设、教学改革理论研究、实验教学内容改革及平台建设、教学团队建设等方面进行系统研究与实践，通过构建新的实践课程体系，进行化学实验课程绿色化改进，逐步形成了林业高校化学学科研究生“四层次一体化”培养新模式。
语种：
中文
展开
氨基吡啶修饰的高交联树脂合成与吸附性能
作者：
王斌;秦海蛟;皮少锋;谢练武;肖谷清
期刊：
化学研究与应用 ,2016年28(4):525-529 ISSN：1004-1656
作者机构：
中南林业科技大学理学院应用化学研究所, 湖南, 长沙, 410004;湖南城市学院化学与环境工程学院, 湖南, 益阳, 413000;[王斌; 秦海蛟; 皮少锋; 谢练武] 中南林业科技大学理学院应用化学研究所, 湖南, 长沙, 410004;[肖谷清] 湖南城市学院化学与环境工程学院, 湖南, 益阳, 413000
关键词：
氨基吡啶;超高交联树脂;水杨酸;吸附
摘要：
以氯球为原料,二氯乙烷为溶剂,FeCl_3为催化剂,利用Friedel-Crafts反应制备甲苯超高交联树脂,测其氯含量为6.38%。为降低氯含量和提高吸附性能,采用2-氨基吡啶对该高交联树脂进行化学修饰,获得的高交联树脂(记为GQ-08)氯含量下降为1.36%,然后详细研究了GQ-08对水杨酸的吸附性能。实验结果表明, GQ-08在pH 2.75时对水杨酸的吸附容量最大,达到310.7 mg·g~(-1);GQ-08对水杨酸的吸附是放热过程,吸附等温线服从Freundlich方程;当溶液中NaCl含量由0%到2%变化时,GQ-08对水杨酸的吸附量明显减少,说明氯离子与水杨酸存在竞争吸附作用,增加盐度不利于水杨酸吸附。因此,在利用GQ-08树脂进行水杨酸富集分离或处理生产废水回收水杨酸时应优先考虑酸度、盐度等工艺参数。
语种：
中文
展开
一种氨基吡啶修饰的甲苯超高交联大孔树脂的制备及性能研究
作者：
谢星雨;王斌;秦海蛟;肖谷清;谢练武
期刊：
化学与生物工程 ,2016年33(1):26-29 ISSN：1672-5425
作者机构：
中南林业科技大学理学院应用化学研究所,湖南长沙410004;长沙市第一中学,湖南长沙410005;湖南城市学院化学与环境工程学院,湖南益阳413000;中南林业科技大学理学院应用化学研究所,湖南长沙,410004;湖南城市学院化学与环境工程学院,湖南益阳,413000
关键词：
氨基吡啶;甲苯超高交联大孔树脂;水杨酸;吸附
摘要：
通过傅克烷基化反应,以氯甲基化聚苯乙烯（CMP）与甲苯反应制备氯含量为6.38%的甲苯超高交联大孔树脂GQ-06,然后采用2-氨基吡啶对GQ-06进行化学修饰,制备氯含量为1.36%的甲苯超高交联大孔树脂GQ-08,并比较了CMP、GQ-06与GQ-08对水杨酸吸附性能的差异。结果表明,相较于CMP与GQ-06,GQ-08对水杨酸的吸附大大增强,并在pH=2.75时对水杨酸的吸附量达最大值310.7mg·g（-1）,分别是CMP与GQ-06的10.1倍与4.1倍;GQ-08对水杨酸的吸附是放热过程,当溶液中NaCl含量增加时,GQ-08对水杨酸的吸附量明显减少,说明氯离子与水杨酸存在竞争吸附作用。2-氨基吡啶修饰的甲苯超高交联大孔树脂吸附水杨酸存在离子交换吸附和酸碱化学吸附两大吸附机理,吸附容量大,可利用GQ-08树脂处理含水杨酸的生产废水来富集回收水杨酸。
语种：
中文
展开
农林院校基于专业特色化学实验课程垂直体系构建研究
作者：
王文磊;谢练武;皮少锋;张宁;王元兰
期刊：
发现 ,2016年(6):43-44 ISSN：1004-5023
作者机构：
中南林业科技大学理学院
摘要：
《国家中长期教育改革和发展规划纲要（2010-2020年）》明确指出，提高质量是高等教育的核心任务，是建设高等教育强国的基本要求。化学实验是对化学相关学科类专业学生进行科学实验基本训练的一门独立的必修基础课程，有助于帮助学生更好地理解化学基本概念和基础理论，培养学生良好的实验技能，激发学生学习化学的兴趣，并且培养学生实事求是、严肃认真的科学态度和科学的学习方法[1]。农林院校主要围绕专业特色鲜明的农学、林学等主要学科及相关学科进行学科建设及学生培养。化学实验中心承担全校无机及分析化学实验、有机化学实验、物理化学实验、仪器分析实验、波谱分析实验、色谱分析实验和光谱分析实验等多门课程实验教学，面向木材科学与技术、材料化学、化学工程与工艺、材料科学与技术、林产化工、食品质量与安全、食品科学与工程、粮食工程、生态、园林、生物技术、生物工程、环境工程、环境科学、林学、园艺近20个专业全日制本科生以及化学工程与技术学科的硕士研究生。每学年进入实验中心实验的学生约为3000人次，实验教学学时5000学时，实验室管理工作144000人次流量。目前，学校所采取的化学实验课程体系存在实验化学内容与专业学科结合不紧密，不同学科实验内容一样，与其专业课程的横向联系较少，没有体现农林院校的专业特色等问题[2,3]。因此，针对我校地方院校性质和以林业为主的专业特点，提出打破现有化学学科传统实验课程教学体系，探索农林院校本科化学实验创新型教学模式，有利于推进地方高等农林院校化学课程体系的改革与创新。
语种：
中文
展开
肠外翻囊法研究淫羊藿肠吸收成分及其吸收特征
作者：
秦海蛟;柳梦婷;唐力英;战皓;方婧;...
期刊：
中国实验方剂学杂志 ,2015年21(22):163-168 ISSN：1005-9903
作者机构：
[秦海蛟; 王斌; 谢练武] 中南林业科技大学理学院;[柳梦婷; 唐力英; 战皓; 方婧; 杨洪军; 付梅红; 吴宏伟] 中国中医科学院中药研究所
关键词：
淫羊藿;外翻肠囊法;朝藿定A;淫羊藿苷;淫羊藿次苷Ⅱ
摘要：
目的:研究淫羊藿肠吸收成分及其吸收特性。方法:采用大鼠外翻肠囊模型,收集高、中、低质量浓度淫羊藿提取物给药后不同时间的肠囊液,采用HPLC检测肠吸收液样品中5种黄酮类成分的含量,计算累计吸收量和吸收速率常数,比较淫羊藿提取物与肠吸收液中指标成分的比例变化情况。结果:提取物中主要黄酮类成分均可在肠吸收液中检测到,针对其中5种主要成分朝藿定A,朝藿定B,朝藿定C,淫羊藿苷与淫羊藿次苷Ⅱ成功建立了定量分析方法。提取液中、低质量浓度时,这5种成分分别在空肠前、中段的累积吸收量最大,而高质量浓度则后移至回肠段; 3种给药质量浓度下,5种成分的肠吸收均为线性吸收(R~2 > 0.9),符合零级吸收速率;吸收速率常数随着给药浓度的增加而增大,提示各成分的体外肠吸收为被动运输;不同质量浓度淫羊藿肠吸收液的成分间比例与原提取物中各成分间比例存在差异。结论:淫羊藿中朝藿定A,朝藿定B,朝藿定C,淫羊藿次苷Ⅱ与淫羊藿苷5种成分均可被小肠吸收,外翻肠囊法可有效地评价各成分的吸收特征。
语种：
中文
展开
木材腐朽真菌定向降解木质素为苯系芳香类化学品
作者：
秦海蛟;付云;文瑞芝;马强;胡云楚;...
期刊：
化工进展 ,2015年(A01):173-177 ISSN：1000-6613
作者机构：
中南林业科技大学理学院，应用化学研究所，湖南 长沙 410004;[谢练武; 秦海蛟; 胡云楚; 马强; 付云; 瑞芝] 中南林业科技大学
关键词：
芳香类化学品;碱木质素;中性木质素;降解;底物依赖性
摘要：
对2种木材腐朽真菌降解木质素为一系列苯系芳香类化学品进行了初步研究,并与黄孢原毛平革菌进行了比较。从木材腐朽土壤样品中分离获得具有降解木质素活性的真菌11株,将其中活性最强的菌株ZS1与ZSH用于降解工业碱木质素与中性木质素。通过发酵条件的优化、降解产物种类与浓度的 HPLC分析,结果发现,在初始底物浓度1.0~20.0g/L变化时,ZS1对碱木质素的降解率达到40.7%,比 ZSH 的大；而 ZSH 对中性木质素的降解率为32.3%,与黄孢原毛平革菌的降解能力相当,但比 ZS1的大些。木质素能被定向降解为香草酸、苯甲酸、香草醛、苯酚、紫丁香醛、苯、对甲基苯甲醚、对甲基苯乙醚、甲苯与对二甲苯等芳香类化学品,而且不同真菌降解不同木质素的产物种类数量与浓度有所差异。这说明不同真菌对木质素的降解具有一定的底物依赖性,底物结构差异影响了降解率不同与产物种类差异,而且不同真菌降解木质素具有一定的产物定向选择性。
语种：
中文
展开
Hydrophilic gallic acid–imprinted polymers over magnetic mesoporous silica microspheres with excellent molecular recognition ability in aqueous fruit juices
作者：
Hu, Xin;Xie, Lianwu;Guo, Junfang;Li, Hui;Jiang, Xinyu;...
期刊：
Food Chemistry ,2015年179:206-212 ISSN：0308-8146
通讯作者：
Shi, Shuyun
作者机构：
[Shi, Shuyun; Zhang, Yuping; Jiang, Xinyu; Li, Hui; Xie, Lianwu; Hu, Xin; Guo, Junfang] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.;[Xie, Lianwu] Cent South Univ Forestry & Technol, Coll Sci, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Shi, Shuyun] C;Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China.
关键词：
Fruit juice;Gallic acid;Hydrophilic molecularly imprinted polymer;Polydopamine;Selective extraction
摘要：
Hydrophilic molecularly imprinted polymers (MIPs) for gallic acid (GA) were prepared with excellent recognition ability in an aqueous solution. The proposed MIPs were designed by self-polymerization of dopamine (DA) on magnetic mesoporous silica (Fe<inf>3</inf>O<inf>4</inf>@SiO<inf>2</inf>@mSiO<inf>2</inf>, MMS) using GA as template. Resulting Fe<inf>3</inf>O<inf>4</inf>@SiO<inf>2</inf>@mSiO<inf>2</inf>@MIPs (MMS-MIPs) were characterized by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), thermo-gravimetric analysis (TGA), Brunauer-Emmett-Teller (BET), vibrating sample magnetometer (VSM), and evaluated by adsorption isotherms/kinetics and competitive adsorption. The adsorption behavior between GA and MMS-MIPs followed Langmuir and Sips adsorption isotherms with a maximum adsorption capacity at 88.7 mg/g and pseudo-second-order reaction kinetics with fast binding (equilibrium time at 100 min). In addition, MMS-MIPs showed rapid magnetic separation (10 s) and stability (retained 95.2% after six cycles). Subsequently, MMS-MIPs were applied for the selective extraction and determination of GA from grape, apple, peach and orange juices (4.02, 3.91, 5.97, and 0.67 μg/g, respectively). Generally, the described method may pave the way towards rationally designing more advanced hydrophilic MIPs. ©2015 Elsevier Ltd. All rights reserved.
语种：
英文
展开
