期刊论文

Xie, Lianwu(xiecsu@126.com)

中文

食品科学

1002-6630

2017

38

20

125-130

10.7506/spkx1002-6630-201720018

2015-192:人社部留学回国人员择优资助重点项目 2014FJ3011:湖南省重点实验室专项 2016XGJSYB01:湖南省重点实验室专项 2015TP4021-5:湖南省重点实验室专项 2012-266:专业综合改革试点项目

本校为第一机构

目的:运用高效液相色谱(high performance liquid chromatography,HPLC)法研究湖南安化黑茶指纹图谱,并对安化黑茶的主要成分进行定量分析,为湖南安化黑茶的成分分析与鉴别提供理论依据。方法:以22个安化黑茶样品与4种外省黑茶(云南普洱茶、湖北青砖茶、广西六堡茶、四川土司黑茶)为实验材料,采用梯度洗脱(A相为0.17%乙酸溶液,B相为乙腈),柱温30 ℃,检测波长280 nm,流速1.0 mL/min,建立安化黑茶HPLC指纹图谱,并进行了相似度评价与主成分分析,确定共有色谱峰。结果:22个安化黑茶样品具有19个共有色谱峰,这些黑茶样品在共有模式下的相似度为0.783~0.958,而广西六堡茶、四川土司黑茶、湖北青砖茶以...

Objective: To fngerprint dark tea from Anhua, Hunan using high performance liquid chromatography (HPLC) and to quantitatively determine its major water-soluble constituents for the purpose of providing theoretical evidence for the identifcation of Anhua black tea. Methods: A total of 22 dark tea samples from Anhua, Hunan and 4 dark tea samples from other provinces (Liupu tea from Guangxi, Tusi tea from Sichuan, brick tea from Hubei and Pu'er tea from Yunnan) were fngerprinted by HPLC. The chromatographic conditions were as follows: gradient elution using 0.17% acetic acid aqueous solution as m...