New Insights of Salicylic Acid into Stamen Abortion of Female Flowers in Tung Tree (Vernicia fordii)
作者:
Liu, Meilan;Li, Wenying;Zhao, Guang;Fan, Xiaoming;Long, Hongxu;...
期刊:
Frontiers in Genetics ,2019年10(APR):316 ISSN:1664-8021
通讯作者:
Zhang, Lin
作者机构:
[Li, Wenying; Zhao, Guang; Fan, Yanru; Liu, Meilan; Long, Hongxu; Fan, Xiaoming; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;[Li, Wenying; Zhao, Guang; Fan, Yanru; Liu, Meilan; Long, Hongxu; Fan, Xiaoming; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha, Hunan, Peoples R China.;[Shi, Mingwang] Henan Inst Sci & Technol, Xinxiang, Peoples R China.
通讯机构:
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Coll Forestry, Key Lab Nonwood Forest Prod State Forestry Adm, Changsha, Hunan, Peoples R China.
关键词:
flower development;programmed cell death;salicylic acid;stamen abortion;tung tree;unisexual flowers
摘要:
Tung tree (Vernicia fordii), an economically important woody oil plant, is a monoecious and diclinous species with male and female flowers on the same inflorescence. The extremely low proportion of female flowers leads to low fruit yield in tung orchards. The female flower normally develops along with stamen abortion; otherwise sterile ovules will be produced. However, little knowledge is known about the molecular basis of the female flower development in tung tree. In this study, integrated analyses of morphological and cytological observations, endogenous phytohormone assay and RNA-seq were conducted to understand the molecular mechanism of the female flower development in tung tree. Cytological observation suggested that the abortion of stamens in female flowers (SFFs) belongs to the type of programmed cell death (PCD), which was caused by tapetum degeneration at microspore mother cell stage. A total of 1,366 differentially expressed genes (DEGs) were identified in female flowers by RNA-seq analysis, of which 279 (20.42%) DEGs were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction. Stage-specific transcript identification detected dynamically expressed genes of important transcription regulators in female flowers that may be involved in PCD and floral organ development. Gene expression patterns revealed that 17 anther and pollen development genes and 37 PCD-related genes might be involved in the abortion of SFF. Further analyses of phytohormone levels and co-expression networks suggested that salicylic acid (SA) accumulation could trigger PCD and inhibit the development of SFF in tung tree. This study provides new insights into the role of SA in regulating the abortion of SFF to develop normal female flowers.
语种:
英文
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Boosting C16 fatty acid biosynthesis of Escherichia coli, yeast and tobacco by tung tree (Vernicia fordii Hemsl.) beta-hydroxyacyl-acyl carrier protein dehydratase gene
作者:
Liu, Meilan;Long, Hongxu;Li, Wengying;Shi, Mingwang;Cao, Heping;...
期刊:
Industrial Crops and Products ,2019年127:46-54 ISSN:0926-6690
通讯作者:
Zhang, Lin;Tan, Xiaofeng
作者机构:
[Zhang, Lin; Tan, Xiaofeng; Liu, Meilan; Long, Hongxu; Li, Wengying] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.;[Shi, Mingwang] Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.;[Cao, Heping] ARS, USDA, Southern Reg Res Ctr, New Orleans, LA USA.
通讯机构:
[Zhang, L; Tan, XF] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.
关键词:
Fatty acid synthase;Gene expression pattern;Heterologous expression;Subcellular localization;Tung tree;β-Hydroxyacyl-acyl carrier protein dehydratase
摘要:
Beta-hydroxyacyl-acyl carrier protein dehydratase (HAD) catalyzes the formation of enoyl-[ACP] from beta -hydroxylacyl-[ACP] and plays a key role in determining fatty acid composition during the process of oil biosynthesis. In this study, only one HAD gene (GenBank KU358903) was identified and isolated from tung tree (Vernicia fordii Hemsl.). The VfHAD contained an open reading frame (ORF) of 663 bp, encoding a polypeptide of 220 amino acid residues. It shared 86, 85, and 83% amino acid identities with the HADs of Hevea brasiliensis, Jatropha curcas, and Ricinus communis, respectively. The VfHAD exhibited all essential catalytic sites and the characteristic “hot-dog” structure of FabZ protein. Quantitative real-time PCR (qRT-PCR) analysis showed that VfHAD mRNA was detected in all tung tree organs, but its transcript was the most abundant in tung seeds at 20 weeks after flowering when oil rapidly accumulated. The VfHAD was localized in chloroplast. Heterologous expression of VfHAD significantly increased C16 fatty acids content in Escherichia coli, yeast, and tobacco. This study suggested that VfHAD may play a key role in fatty acid biosynthesis in tung seeds and could be a useful target for genetic improvement of oil quality. © 2018 Elsevier B.V.
语种:
英文
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Integrative analysis reveals evolutionary patterns and potential functions of SWEET transporters in Euphorbiaceae
作者:
Cao, Yunpeng;Liu, Wenjuan;Zhao, Qiuyuan;Long, Hongxu;Li, Ze;...
期刊:
International Journal of Biological Macromolecules ,2019年139:1-11 ISSN:0141-8130
通讯作者:
Zhang, Lin
作者机构:
[Li, Ze; Cao, Yunpeng; Zhao, Qiuyuan; Liu, Wenjuan; Long, Hongxu; Liu, Meilan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Li, Ze; Cao, Yunpeng; Zhao, Qiuyuan; Liu, Wenjuan; Long, Hongxu; Liu, Meilan; Zhang, Lin] Cent South Univ Forestry & Technol, Coll Forestry, State Forestry Adm, Key Lab Nonwood Forest Prod, Changsha 410004, Hunan, Peoples R China.;[Zhou, Xu] Cent South Univ Forestry & Technol, Coll Landscape Architecture, Changsha 410004, Hunan, Peoples R China.
通讯机构:
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.
关键词:
Vernicia fordii;SWEET genes;Expression;Internal repeat;Sucrose transport
摘要:
As a macromolecular substance, sucrose contributes to the plant growth and development. SWEET genes, a group of sugar transporters, are a recently found plant gene family and play important roles in sugar efflux, pollen nutrition, nectar secretion, phloem transport, and seed development. The SWEET genes have been identified and characterized in some plants, but the systematic study in tung tree (Vernicia fordii) was limited. Here, we identified 121 SWEETs in five Euphorbiaceae, and could be divided into four classes with 20 different motifs. Multiple sequence alignment revealed seven transmembrane helixes (TMHs) in the SWEET proteins which were created by an internal duplication of an ancestral three-TMHs unit, connected by TMH4. This study provides direct evidence for the first time for internal duplication in Euphorbiaceae. The large-scale duplication events represented the main driving force for SWEET family expansion in Euphorbiaceae. In addition, we determined the key VfSWEETs for sucrose transport from source to sink tissues in V. fordii and proposed a possible sucrose transport model, which would be helpful for understanding the mechanism of sucrose transport in V. fordii. This study provided a new insight into the evolution, expression and structural variations of SWEETs in V. fordii and four other Euphorbiaceae. (C) 2019 Published by Elsevier B.V.
语种:
英文
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Identification and analysis of tRNA genes provide new insights into oil biosynthesis in tung tree (Vernicia fordii Hemsl.)
作者:
Chen, Jun;Liu, Wenjuan;Fan, Yanru;Zhou, Xu;Tang, Xinwei;...
期刊:
Industrial Crops and Products ,2019年137:74-80 ISSN:0926-6690
通讯作者:
Zhang, Lin
作者机构:
[Tang, Xinwei; Liu, Wenjuan; Fan, Yanru; Chen, Jun; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.;[Chen, Jun] East China Univ Technol, Nanchang 330013, Jiangxi, Peoples R China.;[Zhou, Xu] Cent South Univ Forestry & Technol, Coll Landscape Architechture, Changsha 410001, Hunan, Peoples R China.
通讯机构:
[Zhang, Lin] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410001, Hunan, Peoples R China.
关键词:
Tung tree;tRNA;tRNA-derived RNA fragments;Anticodon;Lipid biosynthesis
摘要:
Tung tree (Vernicia fordii Hemsl.)is a potential commercial source of biodiesel which is widely planted in China and many other countries. Investigation of the regulatory mechanism of lipid biosynthesis is an essential prerequisite to improve tung tree varieties by genetic engineering. In this study, the transfer RNA (tRNA)genes were identified in four genome-sequenced species (tung tree, rubber tree, castor bean and physic nut)in Euphorbiaceae. Gene clusters were observed for the four species tRNAs. Though tRNAs were highly conserved, contrasting patterns of nucleotide diversity were observed among the tRNA family. A total of 86 differentially expressed tRNA-derived RNA fragments (tRFs)were identified of which the target genes were enriched in lipid biosynthesis by Gene Ontology (GO)enrichment analysis. Interestingly, 11 lipid genes previously reported were identified and found to be regulated by 17 tRFs. A tRF-mRNA regulatory network was constructed, which provides a new insight into the genetic mechanism of lipid biosynthesis in tung tree. © 2019 Elsevier B.V.
语种:
英文
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油桐4个优良家系主要经济性状分析
作者:
李文莹;刘美兰;张琳;谭晓风;张帆航;...
期刊:
经济林研究 ,2018年36(2):29-34 ISSN:1003-8981
作者机构:
[李文莹; 刘美兰; 张琳; 谭晓风; 张帆航] 中南林业科技大学经济林培育与保护省部共建教育部重点试验室,湖南长沙,410004;[王自重] 湖南省永州市森林资源监测中心,湖南永州,425000
关键词:
油桐;优良家系;经济性状;桐油;理化性质
摘要:
为给油桐良种选育提供参考依据,采用田间调查的方法,从湖南省湘西自治区永顺县青坪镇油桐种质资源收集保存库,选取4个油桐优良家系P005、P007、P009、P012作为试验材料,采用化学分析法、索式提油法、色谱分析等方法,对此4个优良家系的果实性状、种仁性状、桐油油脂脂肪酸成分及油脂的理化性质进行了比较分析.测定结果表明:家系P005的不饱和脂肪酸含量最高;家系P007的桐酸含量最高;家系P009的桐油品质最好;家系P012的出油率最高,其综合性状最好.4个优良家系各性状指标值各有高低,综合分析各项指标后认为,优良家系P005和P012均有较高的生产应用价值,不论是作为良种选育的原始材料还是直接用于生产繁殖,都是较好的选择.
语种:
中文
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Enhancing freezing tolerance of Brassica napus L. by overexpression of a stearoyl-acyl carrier protein desaturase gene (SAD) from Sapium sebiferum (L.) Roxb.
作者:
Peng, Dan;Zhou, Bo* ;Jiang, Yueqiao;Tan, XiaoFeng;Yuan, DeYi;...
期刊:
Plant Science ,2018年272:32-41 ISSN:0168-9452
通讯作者:
Zhou, Bo
作者机构:
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
通讯机构:
[Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.
关键词:
Freezing tolerance;Sapium sebiferum (L.) Roxb.;Stearoyl-acyl carrier protein desaturase;Unsaturated fatty acid
摘要:
Sapium sebiferum (L.) Roxb. is an important woody oil tree and traditional herbal medicine in China. Stearoyl-acyl carrier protein desaturase (SAD) is a dehydrogenase enzyme that plays a key role in the transformation of saturated fatty acids into unsaturated fatty acids in oil; these fatty acids greatly influence the freezing tolerance of plants. However, it remains unclear whether freezing tolerance can be regulated by the expression level of SsSAD in S. sebiferum L. Our research indicated that SsSAD expression in S. sebiferum L. increased under freezing stress. To further confirm this result, we constructed a pEGAD-SsSAD vector and transformed it into B. napus L. W10 by Agrobacterium tumefaciens-mediated transformation. Transgenic plants that overexpressed the SsSAD gene exhibited significantly higher linoleic (18:2) and linolenic acid (18:3) content and advanced freezing tolerance. These results suggest that SsSAD overexpression in B. napus L. can increase the content of polyunsaturated fatty acids (PUFAs) such as linoleic (18:2) and linolenic acid (18:3), which are likely pivotal in improving freezing tolerance in B. napus L. plants. Thus, SsSAD overexpression could be useful in the production of freeze-tolerant varieties of B. napus L. © 2018 Elsevier B.V.
语种:
英文
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Increasing branch and seed yield through heterologous expression of the novel rice S-acyl transferase gene OsPAT15 in Brassica napus L.
作者:
Peng, Dan;Tan, Xiaofeng;Zhang, Lin;Yuan, Deyi;Lin, Jianzhong;...
期刊:
Breeding Science ,2018年68(3):326-335 ISSN:1344-7610
通讯作者:
Zhou, Bo
作者机构:
[Zhou, Bo; Jiang, Yueqiao; Peng, Dan] Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Liu, Xuanming; Lin, Jianzhong] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Zhou, Bo] C;[Zhou, Bo] H;[Zhou, Bo] F;Cent South Univ Forestry & Technol, Fac Life Sci & Technol, Changsha 410004, Hunan, Peoples R China.;Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
关键词:
branching;seed yield;OsPAT15;B. napus L.;sulfur acyl transferase
摘要:
Branching is a predominant element in the plant architecture of Brassica napus L. and represents an important determinant of seed yield. OsPAT15 (OsDHHC1), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the OsPAT15 gene from the monocot rice into the dicot B. napus L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene OsPAT15 was determined to have sulfur acyl transferase activity in the akr1Δ yeast mutant in a complementation experiment. Heterologously expressing OsPAT15 transgenic B. napus L. plants were obtained using the Agrobacterium-mediated floral-dip transformation method. As anticipated, OsPAT15 transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, OsPAT15 transgenic plants had increased primary branches (1.58–1.76-fold) and siliques (1.86–1.89-fold), resulting in a significant increase in seed yield (around 2.39– 2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene OsPAT15 in B. napus L. could be used to increase seed yield and produce excellent varieties. © 2018, Japanese Society of Breeding. All rights reserved.
语种:
英文
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本科生导师制实施现状调查与对策研究
作者:
曾艳玲;曾晓峰;张琳
期刊:
长江大学学报(自科版)医学卷 ,2018年15(18):79-81,85 ISSN:1673-1409
作者机构:
中南林业科技大学林学院,湖南长沙,410004;[曾晓峰; 曾艳玲; 张琳] 中南林业科技大学
关键词:
本科生;导师制;现状;对策
摘要:
本科生导师制是实施个性化教育、提高本科生教育质量的重要措施.为了加强本科生导师制的规范化实施,以中南林业科技大学林学专业在校本科生为调查对象,采取问卷调查及个人访谈的方式,针对本科生导师制认知度、满意度、执行情况以及对大学生学习生活的影响等方面进行调查.结果表明,大多数本科生不了解本科生导师制,班主任和科研导师还没有充分履行职责,存在导学断层现象.针对上述问题,提出具体解决对策,即强化导师职能和推行“1+2+1”导师制教学模式.
语种:
中文
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C:N:P stoichiometry of Ericaceae species in shrubland biomes across Southern China: influences of climate, soil and species identity
作者:
Zhang, Qiang;Liu, Qing;Yin, Huajun;Zhao, Chunzhang;Zhang, Lin;...
期刊:
植物生态学报(英文版) ,2018年12(2):346-357 ISSN:1752-9921
通讯作者:
Xie, Zongqiang;Tang, Zhiyao
作者机构:
[Zhang, Qiang; Xu, Wenting; Xiong, Gaoming; Xie, Zongqiang] Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, 20 Nanxincun, Beijing 100093, Peoples R China.;[Zhang, Qiang; Xie, Zongqiang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China.;[Zhao, Chunzhang; Liu, Qing; Yin, Chunying; Yin, Huajun] Chinese Acad Sci, Chengdu Inst Biol, Key Lab Mt Ecol Restorat & Bioresource Utilizat &, Key Lab Sichuan Prov, Chengdu 610041, Sichuan, Peoples R China.;[Zhang, Lin] Chinese Acad Sci, Inst Tibetan Plateau Res, Key Lab Alpine Ecol & Biodivers, Beijing 100101, Peoples R China.;[Zhou, Guoying] Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China.
通讯机构:
[Xie, Zongqiang] C;[Tang, Zhiyao] P;Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, 20 Nanxincun, Beijing 100093, Peoples R China.;Peking Univ, Dept Ecol, Coll Urban & Environm Sci, 5 Yiheyuan Road, Beijing 100871, Peoples R China.;Peking Univ, Key Lab Earth Surface Proc, 5 Yiheyuan Road, Beijing 100871, Peoples R China.
关键词:
biogeographic pattern;phylogenetic effect;closely related species;Ericoid Mycorrhiza;shrub
摘要:
Aims: Carbon (C), nitrogen (N) and phosphorus (P) stoichiometry strongly affect functions and nutrient cycling within ecosystems. However, the related researches in shrubs were very limited. In this study, we aimed to investigate leaf stoichiometry and its driving factors in shrubs, and whether stoichiometry significantly differs among closely related species. Methods: We analyzed leaf C, N and P concentrations and their ratios in 32 species of Ericaceae from 161 sites across southern China. We examined the relationships of leaf stoichiometry with environmental variables using linear regressions, and quantified the interactive and independent effects of climate, soil and species on foliar stoichiometry using general linear models (GLM). Important Findings: The foliar C, N and P contents of Ericaceae were 484.66, 14.44 and 1.06 mg g -1 , respectively. Leaf C, N and P concentrations and their ratios in Ericaceae were significantly related with latitude and altitude, except the N:P insignificantly correlated with latitude. Climate (mean annual temperature and precipitation) and soil properties (soil C, N and P and bulk density) were significantly influenced element stoichiometry. The GLM analysis showed that soil exerted a greater direct effect on leaf stoichiometry than climate did, and climate affected leaf traits mainly via indirect ways. Further, soil properties had stronger influences on leaf P than on leaf C and N. Among all independent factors examined, we found species accounted for the largest proportion of the variation in foliar stoichiometry. These results suggest that species can largely influence foliar stoichiometry, even at a lower taxonomic level. © 2018 The Author(s).
语种:
英文
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油茶长链脂肪酰基CoA合成酶基因1(CoLACS1)分子特征与表达分析
作者:
江南;谭晓风;张琳;李泽;蒋瑶;...
期刊:
植物遗传资源学报 ,2017年18(1):139-147 ISSN:1672-1810
作者机构:
中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[江南; 谭晓风; 张琳; 蒋瑶] 经济林育种与栽培国家林业局重点实验室;[江南; 谭晓风; 张琳; 蒋瑶] 经济林培育与利用湖南省2011协同创新中心, 长沙, 410004;[李泽; 黄丽媛] 经济林育种与栽培国家林业局重点实验室, 长沙, 410004;[江南; 谭晓风; 张琳; 李泽; 蒋瑶; 黄丽媛] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室
关键词:
油茶;长链脂肪酰基CoA合成酶;基因表达;油脂积累
摘要:
长链脂肪酰基CoA合成酶(LACS)在脂肪酸的合成与分解代谢中起着重要作用。本研究以油茶(Camellia oleifera Abel)国家审定品种华硕(Camellia oleifera Huashuo)种仁转录组数据为基础,根据LACS基因Unigene序列设计引物,分离克隆了油茶LACS1基因全长cDNA序列,命名为CoLACS1(GeneBank登录号:KJ960228),全长2114 bp,开放阅读框2088 bp,编码695个氨基酸;生物信息学分析显示CoLACS1具有3个B1ock,从分子特征可判断CoLACS1属于LACS家族;氨基酸同源比对显示与其他物种的LACS氨基酸序列具有较高的相似性,其中与拟南芥LACS7相似性为78%,与麻风树、大豆、毛果杨等物种LACS6(peroxisomal)相似性可达80%以上;对CoLACS1进行原核表达分析,构建的pET30a-CoLACS1载体成功转化至BL21 (DE3)中经1 mmol /L IPTG诱导表达,菌液检测获得预测的目的蛋白(分子量约为76 kD);分析转录组数据中CoLACS1的Unigene序列RTKM值并对CoLACS1进行实时荧光定量PCR分析,结果表明CoLACS1在油茶华硕种子发育各时期平稳表达,表达丰度变化不大,荧光定量结果变化规律与转录组数据分析一致;同时分析华硕种仁不同时期含油率和脂肪酸成分变化,双变量统计分析发现CoLACS1表达模式与油茶油脂积累规律呈显著相关性。本研究为进一步研究油茶油脂积累与代谢的基因调控提供理论依据。
语种:
中文
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干旱胁迫对两种油桐幼苗生长、气体交换及叶绿素荧光参数的影响
作者:
李泽;谭晓风;卢锟;张琳;龙洪旭;...
期刊:
生态学报 ,2017年37(5):1515-1524 ISSN:1000-0933
通讯作者:
Tan, X.F.
作者机构:
[李泽; 谭晓风; 卢锟; 张琳; 龙洪旭; 吕佳斌; 林青] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[李泽; 谭晓风; 卢锟; 张琳; 龙洪旭; 吕佳斌; 林青] 经济林培育与利用湖南省协同创新中心, 长沙, 410004
关键词:
三年桐;千年桐;干旱胁迫;光合特性;光饱和点;叶绿素荧光
摘要:
为了探究干旱胁迫对两种油桐(三年桐和千年桐)幼苗光合生理特性的变化及响应,采用盆栽试验,研究不同水分处理(正常供水、轻度干旱、中度干旱、重度干旱)对油桐幼苗生长、叶片气体交换及叶绿素荧光参数的影响。结果表明:与对照相比,轻度干旱胁迫对两种油桐生长、气体交换及叶绿素荧光参数无明显影响(P>0.05);中度干旱及重度干旱使两种油桐的叶绿素SPAD值、生长量、净光合速率(Pn)、气孔导度(G_s)、蒸腾速率(T_r)、气孔限制值(L_s)、最大净光合速率(P_(nmax))、光饱和点(LSP)、表观量子效率(AQY)、暗呼吸速率(R_d)、最大光化学效率(F_v/F_m)、实际光化学量子效率(Ф_(PSⅡ))、电子传递速率(ETR)及光化学猝灭系数(q_p)显著下降(P<0.05),且在重度干旱胁迫下迅速下降,胞间CO_2浓度(C_i)、水分利用效率(WUE)、光补偿点(LCP)、初始荧光(F_o)、非光化学猝灭系数(NPQ)显著升高(P<0.05);中度干旱胁迫下油桐幼苗P_n的降低是由气孔因素及光合机构活性降低的非气孔因素共同引起的,而重度干旱胁迫下光合作用的下降主要是由光合机构活性降低的非气孔因素引起的。三年桐的光合机构活性及光合效率高于千年桐,对干旱胁迫的适应性较千年桐强。
语种:
中文
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油茶CoPDAT基因的克隆与表达分析
作者:
赵广;宋志波;刘美兰;龙洪旭;李泽;...
期刊:
植物生理与分子生物学学报 ,2017年53(9):1619-1628 ISSN:1671-3877
通讯作者:
Zhang, L.
作者机构:
[赵广; 宋志波; 刘美兰; 龙洪旭; 李泽; 张琳] 中南林业科技大学, 经济林培育与保护教育部重点实验室;[赵广; 宋志波; 刘美兰; 龙洪旭; 李泽; 张琳] 经济林育种与栽培国家林业局重点实验室;[赵广; 宋志波; 刘美兰; 龙洪旭; 李泽; 张琳] 经济林培育与利用湖南省2011协同创新中心, 长沙, 410004
通讯机构:
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
关键词:
基因克隆;生物信息学分析;亚细胞定位;表达模式;灰色关联度
摘要:
磷脂:二酰甘油酰基转移酶(PDAT)能够催化磷脂酰胆碱(PC) sn-2位的酰基转移至二酰甘油(DAG)上,形成三酰甘油(TAG)和溶血磷脂酰胆碱,这是另外一条不同于Kennedy途径的三酰甘油合成方式,故明确油茶(Camellia oleifera) PDAT基因的功能对阐明油茶种子油脂合成机制具有重要意义。本研究通过cDNA末端快速扩增(RACE)技术克隆油茶CoPDAT基因的全长cDNA序列,该序列长2 773 bp,开放阅读框为2 022 bp,编码673个氨基酸。亚细胞定位分析确定CoPDAT基因所编码蛋白质定位于内质网上。实时荧光定量PCR分析结果表明, CoPDAT在油茶不同组织/器官中均有表达,在种子中不同发育时期的相对表达量呈"上升-下降-上升-下降-上升"的表达特征,在花后42周表达量最高。CoPDAT基因与油茶种子油脂含量的灰色关联度值为0.8356,表明该基因与油茶种子油脂的合成积累关系密切。本研究为阐明CoPDAT基因在油茶油脂合成过程中的功能以及为油茶的分子遗传改良提供参考。
语种:
中文
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Studies on Teaching Technologies of Specialty English for Horticulture Major
作者:
Zhang, Lin* ;Tang, Li;Tan, Xiao-feng
作者机构:
[Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Tang, Li] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
会议名称:
2013 International Conference on Advanced Education Technology and Management Science(AETMS2013)
会议时间:
2013-12-01
会议地点:
Hong Kong
会议主办单位:
[Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.^[Tang, Li] Cent South Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
关键词:
Specialty English;Horticulture major;Teaching reform;Teaching methods
摘要:
Horticulture English as an important English style is quite different from non-technical English style. The characteristics of horticulture specialty English was summarized in this paper. On this basi
语种:
英文
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Flower Bud Differentiation and Development of ‘Jinsi No.4’ Jujube ( Mill.) in Hunan Province of Southern China
作者:
Feng Zou;Jinghua Duan;Huan Xiong;Deyi Yuan;Lin Zhang;...
期刊:
The Open Biotechnology Journal ,2017年11(1):9 -15 ISSN:1874-0707
通讯作者:
Yuan, D.
作者机构:
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;Chinese Academy of Forestry, Beijing, 100091, China;Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States
通讯机构:
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China
关键词:
Bud differentiation;Floral organ;fruit crop;Ziziphus jujuba ‘Jinsi No.4’.
摘要:
Ziziphus jujuba Mill. is one of the most important fruit crops and has been cultivated in China for more than 4000 years. Z. jujuba fruit is rich in nutritional and medicinal values. Compared to other wood fruits, Z. jujuba is unique in its flowering and fruiting characteristics. Floral buds differentiation and formation of Z. jujuba is an essential process that affects yield. Z. jujuba ‘Jinsi No.4' blooms profusely, yet its final yield is low. In this study, the floral bud differentiation and development of ‘Jinsi No.4' were examined by paraffin section. Results showed that the floral buds of ‘Jinsi No.4' differentiated in the current year and started from early April. The duration of a single flower differentiation was short, taking only 7 days for maturation of flowers buds. Floral bud differentiation of Jinsi No.4' can be divided into six stages, i.e., pre-differentiation, initial differentiation, sepal differentiation, petal differentiation, stamen differentiation, and pistil differentiation. Flower development experienced seven stages, i.e., alabastrum, alabastrum break, sepal flattening, petal flattening, stamen flattening, filament withering, and ovule swelling. Dysplasia was observed in some floral organs in Z. jujuba Jinsi No.4', suggesting that the dysplasia of floral organs may be one of the main reasons for low yields. Our findings on flower bud development in ‘Jinsi No.4' will contribute to its production and flowering management in Hunan area of southern China. © 2017 Zou et al.
语种:
英文
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The complete chloroplast genome sequence of tung tree (Vernicia fordii): Organization and phylogenetic relationships with other angiosperms
作者:
Li, Ze;Long, Hongxu;Zhang, Lin;Liu, Zhiming;Cao, Heping;...
期刊:
Scientific Reports ,2017年7(1):1869 ISSN:2045-2322
通讯作者:
Tan, Xiaofeng;Shi, Mingwang
作者机构:
[Li, Ze; Liu, Zhiming; Long, Hongxu; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;[Li, Ze; Liu, Zhiming; Long, Hongxu; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilizat Nonwood, Changsha 410004, Hunan, Peoples R China.;[Liu, Zhiming] Eastern New Mexico Univ, Dept Biol, Portales, NM 88130 USA.;[Cao, Heping] ARS, USDA, Southern Reg Res Ctr, New Orleans, LA 70124 USA.;[Shi, Mingwang] Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.
通讯机构:
[Tan, Xiaofeng] C;[Shi, Mingwang] H;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Cooperat Innovat Ctr Cultivat & Utilizat Nonwood, Changsha 410004, Hunan, Peoples R China.;Henan Inst Sci & Technol, Xinxiang 453003, Henan, Peoples R China.
摘要:
Tung tree (Vernicia fordii) is an economically important tree widely cultivated for industrial oil production in China. To better understand the molecular basis of tung tree chloroplasts, we sequenced and characterized its genome using PacBio RS II sequencing platforms. The chloroplast genome was sequenced with 161,528 bp in length, composed with one pair of inverted repeats (IRs) of 26,819 bp, which were separated by one small single copy (SSC; 18,758 bp) and one large single copy (LSC; 89,132 bp). The genome contains 114 genes, coding for 81 protein, four ribosomal RNAs and 29 transfer RNAs. An expansion with integration of an additional rps19 gene in the IR regions was identified. Compared to the chloroplast genome of Jatropha curcas, a species from the same family, the tung tree chloroplast genome is distinct with 85 single nucleotide polymorphisms (SNPs) and 82 indels. Phylogenetic analysis suggests that V. fordii is a sister species with J. curcas within the Eurosids I. The nucleotide sequence provides vital molecular information for understanding the biology of this important oil tree.
语种:
英文
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Increasing seed oil content and altering oil quality of Brassica napus L. by over-expression of diacylglycerol acyltransferase 1 (SsDGAT1) from Sapium sebiferum (L.) Roxb.
作者:
Peng, Dan;Zhang, Lin;Tan, Xiaofeng;Yuan, Deyi;Liu, Xuanming;...
期刊:
Molecular Breeding ,2016年36(10):1-14 ISSN:1380-3743
通讯作者:
Zhou, Bo
作者机构:
[Zhou, Bo; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, Changsha 410018, Hunan, Peoples R China.;[Zhou, Bo; Yuan, Deyi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Cent Cultivat & Utilizat Nonwo, Changsha 410018, Hunan, Peoples R China.;[Liu, Xuanming] Hunan Univ, Key Lab Plant Funct Genom Dev & Regulat, Changsha 410082, Hunan, Peoples R China.;[Zhou, Bo; Peng, Dan] Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
通讯机构:
[Zhou, Bo] F;Forestry Biotechnol Hunan Key Labs, Changsha 410018, Hunan, Peoples R China.
关键词:
Sapium sebiferum L.;SsDGAT1;Clone;Function;Brassica napus L.;Oleic acid content
摘要:
Sapium sebiferum (L.) Roxb. [S. sebiferum] is one of the most important woody oil trees and traditional herbal medicines in China. Diacylglycerol acyltransferases (DGATs) esterify sn-1,2-diacylglycerol with a long-chain fatty acyl-CoA acting as a key enzyme at the last and rate-limiting step of triacylglycerol (TAG) biosynthesis in eukaryotic organisms. Although at least 61 DGAT1 sequences from 48 organisms have been identified, until now there have been almost no reports on the DGAT1 gene in S. sebiferum (SsDGAT1). In an attempt to clarify SsDGAT1’s function, we cloned the CDS (coding sequence) of SsDGAT1 by RACE (rapid amplification of cDNA ends) technology. The full-length CDS of SsDGAT1 contains 1524 bp, encoding a protein of 507 amino acids. Even recombinant SsDGAT1 was able to restore TAG biosynthesis in the yeast strain S. cerevisiae H1246 TAG-deficient mutant. Moreover, when SsDGAT1 was placed under the control of 35S promoter in Brassica napus L. W10, the total oil content of transgenic rape plants showed an increase of 12.3–14.7 % compared with the wild type. In addition, the transgenic rapeseed with heterologous expression of SsDGAT1 showed an alteration in seed oil composition overall: a significant decrease in oleic acid levels but a tendency towards an increase in linolenic acid levels. The result confirmed that SsDGAT1 may be involved in flux control of oil biosynthesis and could be used specifically to manipulate and improve oil content and composition in plants. These experimental findings suggest that we might be able to develop a plant high in industrial oils by over-expression of SsDGAT1 in S. sebiferum. © 2016, Springer Science+Business Media Dordrecht.
语种:
英文
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Characterization of Glycolytic Pathway Genes Using RNA-Seq in Developing Kernels of Eucommia ulmoides
作者:
Feng, Yanzhi;Zhang, Lin* ;Fu, Jianmin;Li, Fangdong;Wang, Lu;...
期刊:
Journal of Agricultural and Food Chemistry ,2016年64(18):3712-3731 ISSN:0021-8561
通讯作者:
Zhang, Lin;Cao, Heping
作者机构:
[Fu, Jianmin; Wang, Lu; Feng, Yanzhi; Li, Fangdong] State Forestry Adm, Paulownia Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.;[Feng, Yanzhi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Mo, Wenjuan] Chinese Acad Forestry, Forestry Expt Ctr North China, Beijing 102300, Peoples R China.;[Cao, Heping] USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA.
通讯机构:
[Zhang, Lin] C;[Cao, Heping] U;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA.
关键词:
Eucommia ulmoides;RNA-seq;glycolytic pathway;quantitative real-time PCR (qPCR);transcriptome;unigene;alpha-linolenic acid
摘要:
Eucommia ulmoides Oliver, the only member of the Eucommiaceae family, is a rare and valuable tree used to produce a highly valued traditional Chinese medicine and contains α-linolenic acid (ALA) up to 60% of the total fatty acids in the kernels (embryos). Glycolysis provides both cellular energy and the intermediates for other biosynthetic processes. However, nothing was known about the molecular basis of the glycolytic pathway in E. ulmoides kernels. The purposes of this study were to identify novel genes of E. ulmoides related to glycolytic metabolism and to analyze the expression patterns of selected genes in the kernels. Transcriptome sequencing based on the Illumina platform generated 96,469 unigenes in four cDNA libraries constructed using RNAs from 70 and 160 days after flowering kernels of both low- and high-ALA varieties. We identified and characterized the digital expression of 120 unigenes coding for 24 protein families involved in kernel glycolytic pathway. The expression levels of glycolytic genes were generally higher in younger kernels than in more mature kernels. Importantly, several unigenes from kernels of the high-ALA variety were expressed more than those from the low-ALA variety. The expression of 10 unigenes encoding key enzymes in the glycolytic pathway was validated by qPCR using RNAs from six kernel stages of each variety. The qPCR data were well consistent with their digital expression in transcriptomic analyses. This study identified a comprehensive set of genes for glycolytic metabolism and suggests that several glycolytic genes may play key roles in ALA accumulation in the kernels of E. ulmoides. ©2016 American Chemical Society.
语种:
英文
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Overexpression of SsDGAT2 from Sapium sebiferum (L.) Roxb Increases Seed Oleic Acid Level in Arabidopsis
作者:
Wang, YiQiang;Peng, Dan;Zhang, Lin;Tan, XiaoFeng;Yuan, DeYi;...
期刊:
Plant Molecular Biology Reporter ,2016年34(3):638-648 ISSN:0735-9640
通讯作者:
Zhou, Bo
作者机构:
[Zhou, Bo; Wang, YiQiang; Peng, Dan] Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Peng, Dan; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;[Zhou, Bo; Tan, XiaoFeng; Yuan, DeYi; Zhang, Lin] Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.;[Liu, XuanMing] Hunan Univ, Key Lab Plant Funct Gnomon Dev & Regulat, Changsha 410082, Hunan, Peoples R China.
通讯机构:
[Zhou, Bo] C;Cent South Univ Forestry & Technol, Coll Biosci & Biotechnol, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Tree, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Collaborat Innovat Ctr Cultivat & Utilizat Nonwoo, Changsha 410004, Hunan, Peoples R China.
关键词:
Arabidopsis;Clone;Function;Oleic acid content;S. sebiferum (L.) Roxb;SsDGAT2
摘要:
Sapium sebiferum (L.) Roxb is one of the most important oil trees in China. Diacylglycerol acyltransferases (DGATs) esterify sn-1, 2-diacylglycerol with a long-chain fatty acyl-CoA, the last step and the rate-limiting step of triacylglycerol (TAG) biosynthesis in prokaryotic and eukaryotic organisms. At least 74 DGAT2 sequences from 61 organisms have been identified, but the SsDGAT2 gene had not been reported to date. To clarify the function of SsDGAT2, we cloned the CDS (rapid amplification of cDNA end) of SsDGAT2 by RACE technology. The full-length CDS of SsDGAT2 contains 1011 bp and encodes a protein of 336 amino acids. Recombinant SsDGAT2 restored TAG biosynthesis to the yeast strain Saccharomyces cerevisiae H1246 TAG-deficient mutant and preferentially incorporated unsaturated C18 fatty acids into lipids. To investigate the biotechnological potential of SsDGAT2, it was expressed under the control of the 35S promoter in Arabidopsis Col-4. The oleic acid content increased by 50 % in transgenic plants relative to the control. The results indicated that most of the oleic acid increase was at the expense of linolenic acid (18:3) content, which suggests that high-oleic-acid-content seeds can be created by the overexpression of SsDGAT2 in S. sebiferum (L.) Roxb.
语种:
英文
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油茶2个甘油-3-磷酸酰基转移酶基因的克隆与组织表达特异性分析
作者:
魏琦琦;赵广;何潇;龙洪旭;李泽;...
期刊:
植物生理与分子生物学学报 ,2016年52(7):1099-1109 ISSN:1671-3877
通讯作者:
Zhang, L.
作者机构:
[魏琦琦; 赵广; 何潇; 龙洪旭; 李泽; 谭晓风; 张琳] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[魏琦琦; 赵广; 何潇; 龙洪旭; 李泽; 谭晓风; 张琳] 经济林育种与栽培国家林业局重点实验室;[魏琦琦; 赵广; 何潇; 龙洪旭; 李泽; 谭晓风; 张琳] 经济林培育与利用湖南省2011协同创新中心, 长沙, 410004
关键词:
油茶;甘油-3-磷酸酰基转移酶;蛋白结构;系统进化树;表达分析
摘要:
甘油-3-磷酸酰基转移酶(glycerol-3-phosphate acyltransferase, GPAT)是三酰甘油生物合成Kennedy途径中的关键酶,在植物的油脂合成与抗逆等方面起着重要作用。本研究在转录组数据基础上,通过RACE技术,从我国重要木本食用油料树种油茶中克隆了2个GPAT基因CoATS1和CoGPAT6的全长cDNA序列,编码序列长度分别为1 353和1 488 bp,分别编码450和495个氨基酸。蛋白结构预测发现2个蛋白C-端都含有1个酰基转移酶结构域, CoGPAT6的N-端还具有1个类卤酸脱卤酶结构域。多序列比对和系统进化分析结果表明, CoATS1与茶树的CsGPAT聚为一类, CoGPAT6与芝麻的SiGPAT6聚为一类。实时荧光定量PCR分析表明, CoATS1和CoGPAT6基因在检测的各样本中均有表达,其中CoATS1在叶片中的表达量显著高于其他组织,在种子的发育过程中,表达呈递减变化; CoGPAT6在花瓣中表达量最高,在种子中呈“降低-升高-降低”的表达特征。本研究为阐明GPAT类基因在油茶种子油脂合成途径中的功能奠定了基础。
语种:
中文
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枣抑制消减cDNA文库构建与分析
作者:
魏琦琦;段经华;冯延芝;李芳东;张琳
期刊:
经济林研究 ,2016年34(4):20-24 ISSN:1003-8981
作者机构:
1. 中南林业科技大学经济林育种与栽培国家林业局重点实验室;2. 中南林业科技大学经济林培育与保护省部共建教育部重点实验室;3. 中南林业科技大学经济林培育与利用湖南省2011协同创新中心;4. 中国林业科学研究院经济林研究开发中心;[李芳东; 冯延芝; 段经华] 中国林业科学研究院
关键词:
枣;抑制消减杂交;cDNA文库;生物信息学分析;功能注释
摘要:
枣树具有独特的开花结果特性,为获得枣花发育基因,以金丝4号枣不同发育时期的花蕾和同时期的叶片cDNA互为试验方和驱动方,利用抑制消减杂交技术分别构建了枣花和枣叶抑制消减杂交cDNA文库(SSH文库)。枣花和枣叶SSH文库重组率分别是92.05%和90.26%,插入片段长度均介于2002 000 bp之间,主要集中于1 000 bp左右。分别从枣花和枣叶SSH文库中随机挑选1 000个阳性克隆进行DNA测序、组装拼接,结果显示枣花SSH文库中获得96条unigene,其中contigs 43个,singletons 53个,96条unigene预测得到73个ORF,经过同源比对分析获得有注释的序列20条,按功能分为7类;枣叶SSH文库中获得86条unigene,其中contigs 45个,singletons 41个,86条unigene预测得到70个ORF,经过同源比对分析获得有23条注释的序列,按功能分为6类。
语种:
中文
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