摘要：
Leucine-rich repeat-containing genes (LRRs) have been reported to play important roles in responses to diseases. However, we poorly understood the response of LRRs to Fusarium wilt infection in tung tree (Vernicia fordii), which is an important economic tree. Here, 437 LRR-containing proteins containing nine types of LRR domains were identified in V. fordii genome. Phylogenetic analysis suggested that nine types of LRR domains could not be divided into separate classes, implying that these LRR domains had a common origin. Totally, 27 LRRs were related to possible resistance to Fusarium wilt after 2, 8, and 13 days post-inoculation. We further found that Vf06G1605 was up-regulate under Fusarium wilt infection after these three time points, Vf10G1602 and Vf02G1413 were up-regulated at 8, and 13 dpi, while Vf07G2320 was down-regulated at these three time points. The WGCNA and promoter elements suggested that WRKY possibly regulate the responses of LRRs to Fusarium wilt infection. This study highlighted the phylogeny and function of LRRs in V. fordii and provided a systematic analysis of these genes in the V. fordii genome. Our results presented here might clearly illustrate physiological mechanisms of resistance to Fusarium wilt infection and the target of marker-assisted breeding in V. fordii. (C) 2020 Elsevier B.V. All rights reserved.
通讯机构：
Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Key Laboratory of Non-wood Forest Products of Forestry Ministry, Central South University of Forestry and Technology, Changsha, China
关键词：
油桐;OFP基因家族;低温胁迫;响应分析
摘要：
OVATE Family Protein(OFP)蛋白是一类在非生物胁迫方面发挥着重要作用的新型植物转录调节因子,目前油桐中尚未报道。本文利用生物信息学的方法对油桐OFP基因家族成员的系统进化、基因结构、启动子元件等进行了预测和分析,并利用转录组数据和qRT-PCR技术研究了该家族成员的组织表达特性及低温响应模式。结果表明,油桐OFP基因家族有12个成员,可划分为4类,都存在OVATE 2结构域;染色体定位发现11个成员不均匀地分布在8条染色体上,且VfOFP6和VfOFP10存在共线性关系;启动子区共含有24类顺式作用元件,其中丰度较大的主要有光响应元件、厌氧诱导元件、激素响应相关元件、低温响应元件等;组织表达特性分析结果表明在根、叶、雌花和两性花、雄花、种子中主要表达的分别是VfOFP10、VfOFP6、VfOFP7、VfOFP12和VfOFP1;在低温胁迫条件下,VfOFP7在根、叶柄、叶中都显著诱导表达,而VfOFP16的表达量则显著下调,推测VfOFP7和VfOFP16可能在油桐响应低温胁迫调控中发挥重要作用,将为下一步开展基因的功能研究和油桐的遗传改良提供参考。
摘要：
Tung tree (Vernicia fordii), an economically important woody oil plant, is a monoecious and diclinous species with male and female flowers on the same inflorescence. The extremely low proportion of female flowers leads to low fruit yield in tung orchards. The female flower normally develops along with stamen abortion; otherwise sterile ovules will be produced. However, little knowledge is known about the molecular basis of the female flower development in tung tree. In this study, integrated analyses of morphological and cytological observations, endogenous phytohormone assay and RNA-seq were conducted to understand the molecular mechanism of the female flower development in tung tree. Cytological observation suggested that the abortion of stamens in female flowers (SFFs) belongs to the type of programmed cell death (PCD), which was caused by tapetum degeneration at microspore mother cell stage. A total of 1,366 differentially expressed genes (DEGs) were identified in female flowers by RNA-seq analysis, of which 279 (20.42%) DEGs were significantly enriched in phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction. Stage-specific transcript identification detected dynamically expressed genes of important transcription regulators in female flowers that may be involved in PCD and floral organ development. Gene expression patterns revealed that 17 anther and pollen development genes and 37 PCD-related genes might be involved in the abortion of SFF. Further analyses of phytohormone levels and co-expression networks suggested that salicylic acid (SA) accumulation could trigger PCD and inhibit the development of SFF in tung tree. This study provides new insights into the role of SA in regulating the abortion of SFF to develop normal female flowers.
关键词：
Vernicia fordii;SWEET genes;Expression;Internal repeat;Sucrose transport
摘要：
As a macromolecular substance, sucrose contributes to the plant growth and development. SWEET genes, a group of sugar transporters, are a recently found plant gene family and play important roles in sugar efflux, pollen nutrition, nectar secretion, phloem transport, and seed development. The SWEET genes have been identified and characterized in some plants, but the systematic study in tung tree (Vernicia fordii) was limited. Here, we identified 121 SWEETs in five Euphorbiaceae, and could be divided into four classes with 20 different motifs. Multiple sequence alignment revealed seven transmembrane helixes (TMHs) in the SWEET proteins which were created by an internal duplication of an ancestral three-TMHs unit, connected by TMH4. This study provides direct evidence for the first time for internal duplication in Euphorbiaceae. The large-scale duplication events represented the main driving force for SWEET family expansion in Euphorbiaceae. In addition, we determined the key VfSWEETs for sucrose transport from source to sink tissues in V. fordii and proposed a possible sucrose transport model, which would be helpful for understanding the mechanism of sucrose transport in V. fordii. This study provided a new insight into the evolution, expression and structural variations of SWEETs in V. fordii and four other Euphorbiaceae. (C) 2019 Published by Elsevier B.V.
关键词：
Anticodon;Lipid biosynthesis;tRNA;tRNA-derived RNA fragments;Tung tree
摘要：
Tung tree (Vernicia fordii Hemsl.) is a potential commercial source of biodiesel which is widely planted in China and many other countries. Investigation of the regulatory mechanism of lipid biosynthesis is an essential prerequisite to improve tung tree varieties by genetic engineering. In this study, the transfer RNA (tRNA) genes were identified in four genome-sequenced species (tung tree, rubber tree, castor bean and physic nut) in Euphorbiaceae. Gene clusters were observed for the four species tRNAs. Though tRNAs were highly conserved, contrasting patterns of nucleotide diversity were observed among the tRNA family. A total of 86 differentially expressed tRNA-derived RNA fragments (tRFs) were identified of which the target genes were enriched in lipid biosynthesis by Gene Ontology (GO) enrichment analysis. Interestingly, 11 lipid genes previously reported were identified and found to be regulated by 17 tRFs. A tRF-mRNA regulatory network was constructed, which provides a new insight into the genetic mechanism of lipid biosynthesis in tung tree.
作者：
李泽 LI Ze;谭晓风 TAN Xiaofeng;卢锟 LU Kun;张琳 ZHANG Lin;龙洪旭 LONG Hongxu;...
期刊：
生态学报,2017年37(5):1515-1524 ISSN：1000-0933
通讯作者：
Tan, X.F.
作者机构：
[李泽 LI Ze; 谭晓风 TAN Xiaofeng; 卢锟 LU Kun; 张琳 ZHANG Lin; 龙洪旭 LONG Hongxu; 吕佳斌 LÜ Jiabin; 林青 LIN Qing] 中南林业科技大学, 经济林培育与保护湖南省省部共建教育部重点实验室;[李泽 LI Ze; 谭晓风 TAN Xiaofeng; 卢锟 LU Kun; 张琳 ZHANG Lin; 龙洪旭 LONG Hongxu; 吕佳斌 LÜ Jiabin; 林青 LIN Qing] 经济林培育与利用湖南省协同创新中心, 长沙, 410004
通讯机构：
Key Lab of Non-wood Forest Products of State Forestry Administration, Cooperative Innovation Center of Cultivation and Utilization for Non-wood Forest Trees of Hunan Province, Central South University of Forestry and Technology, Changsha, China
摘要：
Horticulture English as an important English style is quite different from non-technical English style. The characteristics of horticulture specialty English was summarized in this paper. On this basi
期刊：
The Open Biotechnology Journal,2017年11(1):9-15 ISSN：1874-0707
通讯作者：
Yuan, D.
作者机构：
[Zou F.; Xiong H.; Yuan D.; Zhang L.] The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China;[Duan J.] Chinese Academy of Forestry, Beijing, 100091, China;Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States;[Niu G.] The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China<&wdkj&>Texas A&M AgriLife Research Center at El Paso, Texas A&M University System, 1380 A&M Circle, El Paso, TX 79927, United States
通讯机构：
The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan, China
摘要：
Tung tree (Vernicia fordii) is an economically important tree widely cultivated for industrial oil production in China. To better understand the molecular basis of tung tree chloroplasts, we sequenced and characterized its genome using PacBio RS II sequencing platforms. The chloroplast genome was sequenced with 161,528 bp in length, composed with one pair of inverted repeats (IRs) of 26,819 bp, which were separated by one small single copy (SSC; 18,758 bp) and one large single copy (LSC; 89,132 bp). The genome contains 114 genes, coding for 81 protein, four ribosomal RNAs and 29 transfer RNAs. An expansion with integration of an additional rps19 gene in the IR regions was identified. Compared to the chloroplast genome of Jatropha curcas, a species from the same family, the tung tree chloroplast genome is distinct with 85 single nucleotide polymorphisms (SNPs) and 82 indels. Phylogenetic analysis suggests that V. fordii is a sister species with J. curcas within the Eurosids I. The nucleotide sequence provides vital molecular information for understanding the biology of this important oil tree.
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016年64(18):3712-3731 ISSN：0021-8561
通讯作者：
Zhang, Lin;Cao, Heping
作者机构：
[Fu, Jianmin; Wang, Lu; Feng, Yanzhi; Li, Fangdong] State Forestry Adm, Paulownia Res & Dev Ctr, Zhengzhou 450003, Henan, Peoples R China.;[Feng, Yanzhi; Tan, Xiaofeng; Zhang, Lin] Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;[Mo, Wenjuan] Chinese Acad Forestry, Forestry Expt Ctr North China, Beijing 102300, Peoples R China.;[Cao, Heping] USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA.
通讯机构：
[Zhang, Lin] C;[Cao, Heping] U;Cent South Univ Forestry & Technol, Key Lab Cultivat & Protect Nonwood Forest Trees, Minist Educ, Changsha 410004, Hunan, Peoples R China.;USDA ARS, So Reg Res Ctr, POB 19687, New Orleans, LA 70124 USA.
