期刊论文

Molecular Characterization and Expression Analysis of Long Chain Fatty Acid Acyl-CoA Synthetase Gene 1(CoLACS1) from Oil Camellia(Camellia oleifera C. Abel)

中文

植物遗传资源学报

1672-1810

2017

18

1

139-147

10.13430/j.cnki.jpgr.2017.01.018

国家林业公益性行业科研专项重大项目（201404702） 湖南省自然科学基金项目（2016JJ5031） 国家自然科学基金项目（31470684）

本校为第一机构

长链脂肪酰基CoA合成酶(LACS)在脂肪酸的合成与分解代谢中起着重要作用。本研究以油茶(Camellia oleifera Abel)国家审定品种华硕(Camellia oleifera Huashuo)种仁转录组数据为基础,根据LACS基因Unigene序列设计引物,分离克隆了油茶LACS1基因全长cDNA序列,命名为CoLACS1(GeneBank登录号:KJ960228),全长2114 bp,开放阅读框2088 bp,编码695个氨基酸;生物信息学分析显示CoLACS1具有3个B1ock,从分子特征可判断CoLACS1属于LACS家族;氨基酸同源比对显示与其他物种的LACS氨基酸序列具有较高的相似性,其中与拟南芥LACS7相似性为78%,与麻风树、大豆、毛果杨等物种LACS6(peroxisomal)相似性可达80%以上;对...

Long chain fatty acid acyl-CoA synthetase （LACS）plays an important role in fatty acid anabolism and catabolism. Based on seeds transcriptome Unigene sequence of oil Camellia cultivar Camellia oleifera C. Abel Huashuo（ ＇HS＇ ） ,the cDNA encoding LACS was isolated and cloned from the developing seed,which was designated as CoLACS1 （GeneBank accession number： KJ960228）. CoLACS1 with complete sequence of 2114 bp had an open reading frame with 2088 bp encoding 695 amino acids. Sequence analysis demonstrated that the CoLACS1 possessed three b...