期刊论文

Zhang, L.

中文

植物生理与分子生物学学报

1671-3877

2017

53

9

1619-1628

10.13592/j.cnki.ppj.2017.0030

(31470684). This work was supported by the National Natural Science Foundation of China (Grant 31470684):国家自然科学基金

本校为第一且通讯机构

磷脂:二酰甘油酰基转移酶(PDAT)能够催化磷脂酰胆碱(PC) sn-2位的酰基转移至二酰甘油(DAG)上,形成三酰甘油(TAG)和溶血磷脂酰胆碱,这是另外一条不同于Kennedy途径的三酰甘油合成方式,故明确油茶(Camellia oleifera) PDAT基因的功能对阐明油茶种子油脂合成机制具有重要意义。本研究通过cDNA末端快速扩增(RACE)技术克隆油茶CoPDAT基因的全长cDNA序列,该序列长2 773 bp,开放阅读框为2 022 bp,编码673个氨基酸。亚细胞定位分析确定CoPDAT基因所编码蛋白质定位于内质网上。实时荧光定量PCR分析结果表明, CoPDAT在油茶不同组织/器官中均有表达,在种子中不同发育时期的相对表达量呈"上升-下降-上升-下降...

Phospholipid: diacylglycerol acyltransferase (PDAT) can catalyze the sn-2 acyl of phosphatidylcholine (PC) to transfer to the diacylglycerol (DAG), forming triacylgilcerol (TAG) and lysophosphatidyl choline. PDAT-based TAG synthesis pathway is different from the well-known Kennedy pathway, therefore it is of great significance to clarify the function of PDAT gene in Camellia oleifera. In this study, a specific band of about 800 bp was amplified from C. oleifera seeds by reverse transcription PCR. The full-length cDNA sequence of PDAT from C. ol...