丝裂原活化蛋白激酶基因CfMKK1调控果生炭疽菌的生长发育和致病力
作者：
肖宇;李河
期刊：
微生物学报 ,2021年61(1):141-151 ISSN：0001-6209
作者机构：
南方人工林病虫害防控国家林草局重点实验室,森林有害生物防控湖南省重点实验室,经济林培育与保护教育部重点实验室,中南林业科技大学,湖南长沙410004;[肖宇; 李河] 中南林业科技大学
关键词：
油茶;果生炭疽菌;丝裂原活化蛋白激酶;致病力
摘要：
【目的】由果生炭疽菌引起的炭疽病是油茶的主要病害,造成油茶产量下降。本文研究果生炭疽菌中丝裂原活化蛋白激酶CfMkk1的生物学功能,旨在为解析油茶炭疽病菌的致病机理提供依据。【方法】根据同源重组原理构建CfMKK1基因敲除载体片段,采用PEG介导法将载体导入原生质体中筛选获得突变体菌株DCfmkk1;PCR扩增果生炭疽菌含有启动子的CfMKK1基因回补片段,构建回补载体pYF11::CfMKK1;采用PEG介导法把回补载体转化至突变体的原生质体中,荧光筛选回补菌株??Cfmkk1-C。测定野生型菌株、突变体菌株DCfmkk1及基因回补菌株?Cfmkk1-C在营养生长、附着胞形成、胁迫应答和致病力等生物学表型。【结果】与野生型和回补菌株相比,CfMKK1基因敲除突变体?Cfmkk1菌丝生长速率明显减缓;在含刚果红的PDA培养基上菌丝生长受到明显抑制,无法穿透玻璃纸,丧失了侵染寄主的能力;而且无法形成附着胞。【结论】研究结果表明CfMKK1基因参与调控油茶果生炭疽菌的生长发育、附着胞形成、致病力以及响应外界胁迫过程。
语种：
中文
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Genome-wide transcriptome analysis of Colletotrichum fructicola CfHAC1 regulation of the response to dithiothreitol stress [果生刺盘孢 CfHAC1 调控应答二硫苏糖醇胁迫的转录组分析]
作者：
李司政;李河
期刊：
菌物学报 ,2020年39(10):1886-1896 ISSN：1672-6472
作者机构：
[李司政; 李河] Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
关键词：
油茶;炭疽病;果生刺盘孢;内质网胁迫应答;差异表达基因
摘要：
果生刺盘孢Colletotrichum fructicola是油茶炭疽病优势病原菌。前期研究发现bZIP转录因子CfHac1参与调控该菌的生长发育和致病性。为了揭示转录因子CfHac1调控果生刺盘孢响应内质网压力和致病机理,本研究测定了ΔCfhac1突变体对内质网压力胁迫剂的敏感性,发现突变体对二硫苏糖醇（dithiothreitol,DTT）的耐受性下降,说明CfHAC1基因可能参与调控果生刺盘孢响应内质网压力胁迫过程。进一步利用高通量RNA-seq技术对该病菌野生型菌株和CfHAC1敲除突变体菌株在DTT胁迫下的转录组进行了比较分析,结果表明差异表达基因共有2 680个,其中上调表达基因有1 181个,下调表达基因有1 499个。Gene Ontology功能分析结果显示,差异表达基因主要参与催化活性、结合、代谢过程、细胞过程、细胞成分合成、生物过程调控和应激反应等生物学过程。KEGG功能富集分析表明,上调表达基因主要被富集到核糖体、真核细胞的核糖体生物合成、RNA转运和氰基氨基酸代谢通路中;下调表达基因显著富集在内质网蛋白质加工、N-聚糖生物合成、类固醇合成和蛋白质分泌等通路中。分析发现转录因子CfHac1调控内质网胁迫应答和致病相关基因的表达。本研究提供了在全基因组水平上对CfHAC1基因与内质网压力胁迫应答之间关联的新认识,为阐明果生刺盘孢响应内质网压力胁迫和致病机制奠定了基础。
语种：
中文
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Colletotrichum fructicola STE50 is required for vegetative growth, asexual reproduction, appressorium formation, pathogenicity and the response to external stress
作者：
Chen, Yan-ying;Liu, Jun-ang;Jiang, Shi-qiang;Li, He;Zhou, Guo-ying*
期刊：
Journal of Plant Pathology ,2020年102(2):335-342 ISSN：1125-4653
通讯作者：
Zhou, Guo-ying
作者机构：
[Li, He; Chen, Yan-ying; Jiang, Shi-qiang; Zhou, Guo-ying; Liu, Jun-ang] Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Art, Changsha 410004, Hunan, Peoples R China.;[Li, He; Chen, Yan-ying; Jiang, Shi-qiang; Zhou, Guo-ying; Liu, Jun-ang] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.;[Li, He; Chen, Yan-ying; Jiang, Shi-qiang; Zhou, Guo-ying; Liu, Jun-ang] Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Cultivat & Conservat Minis, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhou, Guo-ying] C;Cent South Univ Forestry & Technol, Key Lab Natl Forestry & Grassland Adm Control Art, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.;Cent South Univ Forestry & Technol, Key Lab Nonwood Forest Cultivat & Conservat Minis, Changsha 410004, Hunan, Peoples R China.
关键词：
Anthracnose;Gene function;Fungus pathogen;Tea oil camellia
摘要：
Colletotrichum fructicola is a fungal pathogen that causes Camellia oleifera anthracnose. Mitogen-activated protein kinase cascade signaling regulates plants defenses and is modulated by Ste50, a scaffold protein that mediates the interaction of Ste11 and Ste7. In this study, the Saccharomyces cerevisiae STE50 homolog CfSTE50 in C. fructicola was identified and disrupted. CfSTE50 encodes a 482 amino acid protein. The mutants showed significant differences in conidial yields and appressorium failed to form. Vegetative growth also decreased compared to wild-type strains. Inoculation with conidial suspensions showed that the virulence of the mutant on wounded leaves was significantly impaired. Further analysis showed that the targeted deletion of CfSTE50 resulted in enhanced sensitivity to external peroxide stress, but reduced sensitivity to cell wall stress. These results provide evidence that CfSte50 regulates the growth and development of C. fructicola and regulates asexual reproduction, appressorium formation, pathogenicity and the response to external stresses. © 2019, Società Italiana di Patologia Vegetale (S.I.Pa.V.).
语种：
英文
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调控油茶果生刺盘孢bZIP转录因子CfAp1的生物学功能
作者：
高亚兰;何苑皋;李河
期刊：
林业科学 ,2020年56(9):30-39 ISSN：1001-7488
作者机构：
[高亚兰; 何苑皋; 李河] Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Changsha, 410004, China
关键词：
油茶;果生刺盘孢菌;bZIP转录因子;致病力
摘要：
【目的】研究油茶炭疽病的主要流行致病菌果生刺盘孢菌bZIP转录因子CfAp1的生物学功能,以期阐明果生刺盘孢菌致病的分子机制,为油茶炭疽病的防治提供理论依据。【方法】提取果生刺盘孢菌全基因组DNA,根据同源重组原理构建CfAP1基因敲除载体片段,采用PEG介导法把该片段转化至果生刺盘孢菌野生型菌株的原生质体中,验证筛选突变体菌株; PCR扩增果生刺盘孢菌含有启动子的CfAP1基因回补片段,构建回补载体pYF11: : CfAP1;采用PEG介导法把回补载体pYF11: : CfAP1转化至果生刺盘孢菌突变体菌株的原生质体中,获得回补菌株ΔCfap1 /AP1。测定野生型菌株、突变体菌株ΔCfap1-8及基因回补菌株ΔCfap1 /AP1在生长发育、附着胞形成、外界胁迫和致病力等生物学表型。【结果】果生刺盘孢菌中具有1个与灰色大角间座壳(稻瘟菌) bZIP转录因子MoAp1直系同源的基因,命名为CfAP1;该基因全长1 804 bp,编码566个氨基酸,该蛋白含有1个碱性亮氨酸链( bZIP)结构域、2个PAP1结构域和2个未知功能的结构域;与野生型和回补菌株相比,突变体ΔCfap1-8生长速率没有明显影响,但气生菌丝显著减少;通过测量产孢量,发现突变体ΔCfap1-8的分生孢子显著减少; ΔCfap1-8在含2.5、5 mmol·L~(-1) H_2O_2和0.7 mol·L~(-1)NaCl的PDA平板上菌丝生长受到明显抑制;致病力测试结果表明,果生刺盘孢菌基因敲除突变体ΔCfap1-8对无伤和有伤油茶叶片的致病力下降明显,与野生型和回补菌株的致病力差异显著;突变体分生孢子产生附着胞数量减少且膨压降低。【结论】转录因子CfAp1参与调控油茶果生刺盘孢菌的生长发育、产孢、致病力以及响应外界氧压胁迫和渗透压胁迫过程。
语种：
中文
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First Report of Colletotrichum siamense Causing Anthracnose on Rosa chinensis in China
作者：
Feng, F. S.;Zhou, G. Y.;Li, H.*
期刊：
PLANT DISEASE ,2019年103(6):1422-1422 ISSN：0191-2917
通讯作者：
Li, H.
作者机构：
[Feng, F. S.; Zhou, G. Y.; Li, H.] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Nonwood Forest Cultivat & Conservat, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li, H.] C;Cent South Univ Forestry & Technol, Minist Educ, Key Lab Nonwood Forest Cultivat & Conservat, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
关键词：
fungi;herbaceous/flowering plants;disease management;pathogen detection
语种：
英文
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Functional analysis of CfSnf1 in the development and pathogenicity of anthracnose fungus Colletotrichum fructicola on tea-oil tree
作者：
Zhang, Shengpei;Guo, Yuan;Li, Sizheng;Zhou, Guoying;Liu, Junang;...
期刊：
Bmc Genetics ,2019年20(1):1-9 ISSN：1471-2156
通讯作者：
Li, He
作者机构：
[Zhang, Shengpei; Liu, Junang; Li, He; Guo, Yuan; Zhou, Guoying; Li, Sizheng] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.;[Zhang, Shengpei; Liu, Junang; Li, He; Guo, Yuan; Zhou, Guoying; Li, Sizheng] Key Lab Natl Forestry & Grassland Adm Control Art, Changsha, Peoples R China.;[Zhang, Shengpei; Liu, Junang; Li, He; Guo, Yuan; Zhou, Guoying; Li, Sizheng] Minist Educ, Hunan Prov Key Lab Control Forest Dis & Pests, Key Lab Nonwood Forest Cultivat & Conservat, Changsha, Peoples R China.;[Xu, Jianping] McMaster Univ, Dept Biol, Hamilton, ON, Canada.
通讯机构：
[Li, He] C;[Li, He] K;[Li, He] M;Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.;Key Lab Natl Forestry & Grassland Adm Control Art, Changsha, Peoples R China.
关键词：
Conidiation;Appressorium formation;Pathogenicity;C. fructicola
摘要：
Tea-oil tree (Camellia oleifera) is a unique edible-oil tree in China, and anthracnose occurs in wherever it is cultivated, causing great economic losses each year. We have previously identified the Ascomycete fungus Colletotrichum fructicola as the major pathogen of anthracnose in Ca. oleifera. The purpose of this study was to characterize the biological function of Snf1 protein, a key component of the AMPK (AMP-activated protein kinase) pathway, for the molecular pathogenic-mechanisms of C. fructicola. We characterized CfSnf1 as the homolog of Saccharomyces cerevisiae Snf1. Targeted CfSNF1 gene deletion revealed that CfSnf1 is involved in the utilization of specific carbon sources, conidiation, and stress responses. We further found that the ΔCfSnf1 mutant was not pathogenic to Ca. oleifera, resulting from its defect in appressorium formation. In addition, we provided evidence showing crosstalk between the AMPK and the cAMP/PKA pathways for the first time in filamentous fungi. This study indicate that CfSnf1 is a critical factor in the development and pathogenicity of C. fructicola and, therefore, a potential fungicide target for anthracnose control.
语种：
英文
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Transcriptome sequencing and comparative analysis between male and female floral buds of the persimmon (Diospyros kaki Thunb.)
作者：
Li, Shuzhan;Sun, Peng* ;Du, Gaigai;Wang, Liyuan;Li, Huawei;...
期刊：
Scientia Horticulturae ,2019年246:987-997 ISSN：0304-4238
通讯作者：
Sun, Peng;Fu, Jianmin
作者机构：
[Du, Gaigai; Mai, Yini; Fu, Jianmin; Wang, Liyuan; Diao, Songfeng; Suo, Yujing; Sun, Peng; Han, Weijuan; Li, Shuzhan; Li, Fangdong] Chinese Acad Forestry, Key Lab Nontimber Forest Germplasm Enhancement &, Nontimber Forest Res & Dev Ctr, 3 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China.;[Li, Huawei; Li, Shuzhan] Cent South Univ Forestry & Technol, Minist Educ, Key Lab Cultivat & Protect Nonwood Forest Trees, 498 Shaoshan South Rd, Changsha 410004, Hunan, Peoples R China.;[Sun, P; Fu, JM] Chinese Acad Forestry, Nontimber Forest Res & Dev Ctr, 3 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China.
通讯机构：
[Sun, P; Fu, JM] C;Chinese Acad Forestry, Nontimber Forest Res & Dev Ctr, 3 Weiwu Rd, Zhengzhou 450003, Henan, Peoples R China.
关键词：
Diospyros kaki;Sex differentiation;Transcriptome;RNA sequencing;Crucial genes;Gene expression patterns
摘要：
Although the OGI/MeGI system was reported to be essential in the sex determination of persimmons (Diospyros kaki Thunb.), the detailed regulatory mechanism of sex differentiation requires further investigation. In this study, RNA sequencing and comparative analysis between male and female floral buds collected from the same D. kaki ‘Zenjimaru’ tree were conducted to gain insight into gene expression profiles. In total, 1228 genes were differentially expressed between male and female floral buds. Based on gene ontology, Kyoto Encyclopedia of Genes and Genomes pathway analysis, and BLAST analysis, 25 differentially expressed genes involved functionally in phytohormone biosynthesis and signaling transduction, development of floral organs, transcription regulation, programmed cell death (PCD), and other processes were identified as crucial in the regulation of sex differentiation. Among the 25 genes, 15 were upregulated in male floral buds compared with female floral buds, whereas the other 10 were downregulated. Subsequently, the expression patterns of six crucial genes homologous to MeGI, GA20OX2, ACO, IAA32, beta-amyrin 28-oxidase-like, and HEC1 were determined in male and female floral buds during development with the use of real-time quantitative PCR. The results revealed that the genes homologous to IAA32, beta-amyrin 28-oxidase-like, and HEC1 showed male promoting effects from early June to the following late March. The gene homologous to GA20OX2 may stimulate the development of male floral buds in April. In contrast, the genes homologous to MeGI and ACO showed female promoting effects from early April to early May. The transcriptome datas provide comprehensive gene profiles for sex differentiation in D. kaki, as well as new directions and information for further studies in this field. © 2018
语种：
英文
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油茶苗圃炭疽病原菌鉴定及抗药性
作者：
李河;李司政;王悦辰;刘君昂;徐建平;...
期刊：
林业科学 ,2019年55(5):85-94 ISSN：1001-7488
通讯作者：
Zhou, G.
作者机构：
[李河; 李司政; 王悦辰; 刘君昂; 徐建平; 周国英] Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, China
通讯机构：
Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Changsha, China
关键词：
油茶;炭疽病;病原种类;抗药性
摘要：
【目的】探讨中国油茶苗圃炭疽病原菌种类,揭示油茶苗圃炭疽病菌抗药性,为其防治提供依据和指导。 【方法】分离油茶苗圃炭疽病菌,并对分离菌株的ApMat基因进行PCR扩增和测序,通过对序列系统发育分析鉴定炭疽病原菌种类;采用区分剂量法测定所有菌株对杀菌剂多菌灵、乙霉威、戊唑醇和咪鲜胺的抗药性。【结果】湖南、江西、海南和广东4个省份分离的95株的油茶苗圃炭疽病病原菌主要是果生炭疽菌(Colletotrichum fructicola) 、暹罗炭疽菌(C. siamense) 、山茶炭疽菌(C. camelliae)和胶胞炭疽菌(C. gloeosporioides) 4个种,其中炭疽病菌分布范围最广、分离数量最多(83株,占总分离菌株的87.4%) 。抗药性测定结果表明,对多菌灵产生抗性的菌株有31个,占总分离菌株的32.6%;对戊唑醇产生了抗性的菌株有31株,占总分离菌株的32.6%。其中对多菌灵和戊唑醇这2种杀菌剂均具有抗性的菌株有6株,占总分离菌株的6.3%;对多菌灵、乙霉威和戊唑醇3种杀菌剂均有抗性的菌株有2株,占所有分离菌株的2.1%。但所有95个菌株对咪鲜胺敏感,说明目前苗圃油茶炭疽病菌尚未对咪鲜胺产生抗药性。29个高抗多菌灵而对乙霉威敏感菌株第198位氨基酸由谷氨酸(Glu)突变为丙氨酸(Ala),2株对多菌灵和乙霉威具有双重抗药性的菌株第200位氨基酸由苯丙氨酸(Phe)突变为酪氨酸(Tyr) 。 【结论】我国油茶苗圃炭疽病菌已对多菌灵/乙霉威和戊唑醇产生严重的抗药性,但对咪鲜胺尚未检测到抗药性。
语种：
中文
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First Report of Fusarium proliferatum Causing Trunk Canker on Ilex cornuta in China
作者：
Feng, F. S.;Li, H.*
期刊：
PLANT DISEASE ,2019年103(1):155-155 ISSN：0191-2917
通讯作者：
Li, H.
作者机构：
[Feng, F. S.; Li, H.] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Key Lab Nonwood Forest Cultivat & Conservat, Minist Educ, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li, H.] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Key Lab Nonwood Forest Cultivat & Conservat, Minist Educ, Changsha 410004, Hunan, Peoples R China.
语种：
英文
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bZIP转录因子CfHac1参与调控果生刺盘孢菌的生长发育和致病力
作者：
姚权;郭源;魏丰园;李司政;张盛培;...
期刊：
菌物学报 ,2019年38(10):1643-1652 ISSN：1672-6472
作者机构：
[姚权; 郭源; 魏丰园; 李司政; 张盛培; 李河] Key Laboratory of National Forestry and Grassland Administration for Control of Diseases and Pests of South Plantation, Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, Hunan, Changsha, 410004, China
关键词：
油茶;果生刺盘孢菌;致病力
摘要：
油茶炭疽病是油茶Camellia oleifera上最重要病害之一,引起该病害的主要致病菌为果生刺盘孢菌Colletotrichum fructicola.本研究以果生刺盘孢菌bZIP类转录因子CfHac1为研究对象,研究其在果生刺盘孢菌的营养生长、产孢量、附着胞形成、致病力及耐受性等方面的生物学功能,为油茶炭疽病的防控提供理论依据.研究结果表明,果生刺盘孢菌中具有一个与灰色大角间座壳(稻瘟菌)bZIP转录因子MoHac1直系同源的基因,命名为CfHAC1.该基因全长1 627bp,编码526个氨基酸,该蛋白含有一个碱性亮氨酸链(bZlP)结构域和3个未知功能结构域.CfHAC1基因敲除突变体的菌丝生长速度显著变慢,分生孢子产量显著减少且不能正常形成附着胞,并对山梨糖醇和KCI渗透压胁迫敏感性增加;致病力测试结果表明,果生刺盘孢菌基因敲除突变体ΔCfhac1对油茶的致病力显著下降.转录因子CfHac1参与调控果生刺盘孢菌的生长、产孢、附着胞的形成、致病力以及响应外界渗透压胁迫过程.
语种：
中文
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Comparing height–age and height–diameter modelling approaches for estimating site productivity of natural uneven-aged forests
作者：
Fu, Liyong;Lei, Xiangdong;Sharma, Ram P.;Li, Haikui;Zhu, Guangyu;...
期刊：
FORESTRY ,2018年91(4):419-433 ISSN：0015-752X
通讯作者：
Tang, Shouzheng
作者机构：
[Tang, Shouzheng; Duan, Guangshuang; Lei, Yuancai; Guo, Hong; Fu, Liyong; Lei, Xiangdong; Hong, Lingxia; Li, Haikui] Chinese Acad Forestry, Res Inst Forest Resource Informat Tech, Beijing 100091, Peoples R China.;[Fu, Liyong] Penn State Univ, Ctr Stat Genet, Loc T3436,Mailcode CH69,500 Univ Dr, Hershey, PA 17033 USA.;[Sharma, Ram P.] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Prague 6, Suchdol, Czech Republic.;[Zhu, Guangyu] Cent South Univ Forestry & Technol, Coll Forestry, 498 Shaoshan Nanlu, Changsha 410004, Hunan, Peoples R China.;[Li, Yutang] Acad Forest Inventory & Planning Jilin Prov, Changchun 130022, Jilin, Peoples R China.
通讯机构：
[Tang, Shouzheng] C;Chinese Acad Forestry, Res Inst Forest Resource Informat Tech, Beijing 100091, Peoples R China.
摘要：
Accurate estimates of forest site productivity are an essential part of forest management. Only a few approaches exist for estimating site productivity of natural uneven-aged forests. This study compared two approaches: dominant height (DH)-dominant diameter (DD) modelling and DH-stand age (A) modelling in terms of their prediction accuracy for site productivity. We developed the models based on both the algebraic difference approach (ADA) and ADA with mixed-effects modelling. Data originated from four sets of continuous measurements on natural Mongolian Oak (Quercus mongolica Fisch.) and Korean Larch (Larix olgensis Henry.) in northeastern China. The leave-one-out cross-validation was applied to evaluate the models. The results showed that the prediction accuracies of the DH-DD models were significantly higher than that of the DH-A models. The inclusion of block-level random effects significantly increased the accuracies of both the DH-A models and DH-DD models. Compared with the DH-A models, the DH-DD models did not require age measurements which is time-consuming and difficult, but the DD and DH measurements are available from the routine inventories and compatible with the existing forest inventory data. Therefore, the nonlinear mixed-effects DH-DD models are recommended to estimate site productivity of the natural forests. © Institute of Chartered Foresters, 2017. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
语种：
英文
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海南台农芒采后果实炭疽病病原鉴定
作者：
陈亭妤;李聪;周国英;李河
期刊：
热带作物学报 ,2018年39(7):1396-1401 ISSN：1000-2561
作者机构：
[陈亭妤; 李聪; 周国英; 李河] 中南林业科技大学林学院, 森林有害生物防控湖南省重点实验室, 湖南, 长沙, 410004
关键词：
芒果;炭疽病;刺盘孢属真菌;多基因系统发育
摘要：
刺盘孢属（Colletotrichum）真菌是芒果的重要病原菌。本研究采用形态和分子生物学鉴定了海南省台农芒果实采收后炭疽病的病原菌。从台农芒发病果实上总共分离纯化获得38株刺盘孢属真菌。基于形态学特征和多基因序列（核糖体转录间隔区、钙调蛋白、3-磷酸甘油醛脱氢酶、谷氨酰胺合成酶、肌动蛋白、微管蛋白）,确定引起台农芒采后炭疽病的病原为暹罗炭疽菌（C.siamense）、亚洲炭疽菌（C.asianum）和果生炭疽菌（C.fructicola）。其中果生炭疽菌是我国芒果炭疽病原的首次报道。
语种：
中文
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First Report of Leaf Anthracnose Caused by Colletotrichum karstii on Tea-Oil Trees (Camellia oleifera) in China
作者：
Jiang, S. Q.* ;Li, H.
期刊：
PLANT DISEASE ,2018年102(3):674 ISSN：0191-2917
通讯作者：
Jiang, S. Q.
作者机构：
[Jiang, S. Q.; Li, H.] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Jiang, S. Q.] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
语种：
英文
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First Report of Colletotrichum henanense Causing Anthracnose on Tea-Oil Trees in China
作者：
Li, H.;Zhou, G. Y.* ;Qi, X. Y.;Jiang, S. Q.
期刊：
PLANT DISEASE ,2018年102(5):1040-1040 ISSN：0191-2917
通讯作者：
Zhou, G. Y.
作者机构：
[Jiang, S. Q.; Li, H.; Zhou, G. Y.; Qi, X. Y.] Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Zhou, G. Y.] C;Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410004, Hunan, Peoples R China.
语种：
英文
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湖南省油茶炭疽病病原鉴定
作者：
李河;李杨;蒋仕强;刘君昂;周国英
期刊：
林业科学 ,2017年53(8):43-53 ISSN：1001-7488
通讯作者：
Zhou, Guoying
作者机构：
[李河; 李杨; 蒋仕强; 刘君昂; 周国英] 中南林业科技大学, 森林有害生物防控湖南省重点实验室;[李河; 李杨; 蒋仕强; 刘君昂; 周国英] 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004
关键词：
油茶;炭疽病;病原菌
摘要：
【目的】研究湖南省油茶炭疽病病原,为该病害的防控提供依据。【方法】采集湖南省长沙、株洲、浏阳、永州、怀化、常德、常宁等油茶主产区9个样地的典型油茶炭疽病叶,分离纯化叶片病健交界处的真菌菌株,观察菌株在（PDA）培养基上的菌落、分生孢子及附着孢的形态特征,确定为炭疽属真菌。进一步对炭疽属菌株进行柯赫氏法则验证,确定油茶炭疽病病原菌菌株。对获得病原菌的r DNA-ITS、CAL和GAPDH 3个基因进行PCR扩增和测序,得到的基因序列与GenBank中已有模式炭疽属菌株序列按照3个基因一致的顺序拼接后采用PAUP和MrBayes软件构建系统发育树。【结果】从湖南省9个采样地共分离获得62株炭疽属菌株,致病性测试表明这62株菌均能对油茶嫩叶和果致病,但不同菌株的发病时间具有一定差异;根据形态特征及多基因系统发育分析,最终明确湖南省油茶炭疽病的病原种类。【结论】湖南省油茶炭疽病的病原菌包含果生炭疽菌、暹罗炭疽菌、胶孢炭疽菌、山茶炭疽菌和哈锐炭疽菌5种炭疽属真菌,其中果生炭疽菌分布范围最广,分离率最高,达到64.5%。
语种：
中文
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油茶暹罗刺盘孢菌群体遗传结构分析
作者：
李杨;李河;周国英;蒋越西;蒋仕强;...
期刊：
植物保护 ,2017年43(3):49-54 ISSN：0529-1542
作者机构：
中南林业科技大学, 森林有害生物防控湖南省重点实验室;[李杨; 李河; 周国英; 刘君昂] 经济林培育与保护湖南省省部共建教育部重点实验室, 长沙, 410004;中南林业科技大学, 森林有害生物防控湖南省重点实验室, 长沙, 410004;[李杨; 李河; 周国英; 刘君昂] 中南林业科技大学, 森林有害生物防控湖南省重点实验室;[蒋越西; 蒋仕强] 中南林业科技大学, 森林有害生物防控湖南省重点实验室, 长沙, 410004
关键词：
暹罗刺盘孢菌;地理种群;多基因序列;群体遗传结构
摘要：
暹罗刺盘孢菌是油茶炭疽病病原之一,在我国多个油茶产区均有分布。研究油茶暹罗刺盘孢菌群体遗传结构可为全面、有效防治油茶炭疽病害提供理论依据。本研究对分离自海南、江西、湖南、广西4省(自治区)6个地区暹罗刺盘孢菌菌株的ITS、CAL和GAPDH 3个基因的序列进行群体遗传结构分析。根据拼接的上述3个基因的序列,57个暹罗刺盘孢菌菌株可定义为13个单倍型,其中单倍型H7为主要单倍型,分布于本研究所涉及的所有地区。病菌不同地理种群间的遗传分化较大,AMOVA分析显示,遗传变异主要发生在种群内,病菌未经历过大规模的种群扩张。研究结果表明油茶炭疽病原暹罗刺盘孢菌种群具有丰富的遗传多样性。
语种：
中文
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土沉香根结线虫病的病原鉴定
作者：
苏圣淞;周国英;李河;王姣;何苑皞;...
期刊：
植物保护 ,2017年43(4):185-189,194 ISSN：0529-1542
作者机构：
[苏圣淞; 周国英; 李河; 王姣; 何苑皞; 刘君昂] 中南林业科技大学, 经济林培育与保护教育部重点实验室;[苏圣淞; 周国英; 李河; 王姣; 何苑皞; 刘君昂] 森林有害生物防控湖南省重点实验室, 长沙, 410004
关键词：
土沉香;象耳豆根结线虫
摘要：
本研究对海南省澄迈县土沉香根结线虫病危害与发生情况进行了调查与研究,并采用比较形态学结合rDNA-ITS序列分析的方法对病原进行了分离鉴定,确定了发生在澄迈县土沉香苗木上的根结线虫为象耳豆根结线虫Meloidogyne enterolobii Yang & Eisenback。这是首次报道海南省澄迈地区土沉香上发生象耳豆根结线虫,为进一步分析土沉香病原根结线虫种群及该病的防治研究奠定了基础。
语种：
中文
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绿色荧光蛋白标记枯草芽孢杆菌Y13~(UV)在油茶体内的定殖
作者：
金勤;朱丹雪;周国英;李河;何苑皞;...
期刊：
林业科学 ,2017年53(7):111-117 ISSN：1001-7488
通讯作者：
Zhou, Guoying
作者机构：
[何苑皞; 金勤; 张茜; 朱丹雪; 周国英; 李河] Hunan Provincal Key Laboratoty for Control of Forest Disease and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forest and Technology, Changsha, 410004, China;[张茜] Hunan Automotive Engineering Vocational College, Zhuzhou, 412001, China
通讯机构：
Hunan Provincal Key Laboratoty for Control of Forest Disease and Pests, Key Laboratory for Non-Wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forest and Technology, Changsha, China
关键词：
绿色荧光蛋白;枯草芽孢杆菌;油茶炭疽病;定殖
摘要：
【目的】枯草芽孢杆菌Y13~(UV)对油茶炭疽病具有良好的防治效果,研究其在油茶体内的定殖动态,为油茶炭疽病的防治提供依据。【方法】通过原生质体转化法引入绿色荧光蛋白质粒,构建荧光标记菌株。通过喷叶、灌根、喷叶灌根结合处理多种接种方式及重复接种,测定菌株在油茶体内不同组织部位的定殖数量,分析其定殖规律及能力。【结果】标记菌株可以在油茶根、茎和叶内定殖,单次接种当天,根内回收的标记菌株数量为1. 07 × 10~5 cfu·g~(-1);喷叶灌根结合处理7天后,油茶根、茎和叶内标记菌株的数量分别为8. 70 × 10~2、5. 00 × 10~2和7. 30 × 10~2 cfu·g~(-1),均高于喷叶、灌根单独处理。重复接种时,油茶根茎叶内标记菌株的定殖量在接种3 ~ 5天内达到高峰,然后呈现稳定趋势,20天时定殖量开始大幅下降,第30天喷叶灌根处理的油茶根内的定殖量仅为5. 30 × 10~2 cfu· g - 1。荧光标记菌株生长较好,稳定表达,对炭疽病菌具有良好的抑制效果。【结论】枯草芽孢杆菌Y13~(UV)能通过喷叶灌根方式接种在油茶体内定殖并传导,有较好的定殖能力。
语种：
中文
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A Basal Area Increment-Based Approach of Site Productivity Evaluation for Multi-Aged and Mixed Forests
作者：
Fu, Liyong;Sharma, Ram P.;Zhu, Guangyu;Li, Haikui;Hong, Lingxia;...
期刊：
Forests ,2017年8(4):119- ISSN：1999-4907
通讯作者：
Tang, Shouzheng
作者机构：
[Tang, Shouzheng; Duan, Guangshuang; Lei, Yuancai; Guo, Hong; Fu, Liyong; Hong, Lingxia; Lei, Xiangdong; Li, Haikui] Chinese Acad Forestry, Res Inst Forest Resource Informat Tech, Beijing 100091, Peoples R China.;[Fu, Liyong] Penn State Univ, Ctr Stat Genet, Loc T3436,Mail Code CH69,500 Univ Dr, Hershey, PA 17033 USA.;[Sharma, Ram P.] Czech Univ Life Sci Prague, Fac Forestry & Wood Sci, Prague 16521 6, Suchdol, Czech Republic.;[Zhu, Guangyu] Cent South Univ Forestry & Technol, Coll Forestry, 498 ShaoshanNanlu, Changsha 410004, Hunan, Peoples R China.;[Shen, Chenchen] Univ Idaho, Dept Forest Rangeland & Fire Sci, Moscow, ID 83844 USA.
通讯机构：
[Tang, Shouzheng] C;Chinese Acad Forestry, Res Inst Forest Resource Informat Tech, Beijing 100091, Peoples R China.
关键词：
basal area realized productivity;basal area potential productivity;mixed forests;site quality evaluation
摘要：
Accurate estimates of forest site productivity are essential for environmental planning and forest management. In this study, we developed a new productivity index, hereafter termed basal area potential productivity index (BAPP), to estimate site productivity for irregular and complex forests characterized by multi-aged, multi-species, and multi-layer stands. We presented the biological relevance of BAPP with its computational details. We also compared BAPP against basal area realized productivity (BARP) in order to verify the practicability and reliability of BAPP. Time-series data of the national forest inventory on 1912 permanent sample plots that were located in two main forest types and consisted of oak-dominated mixed forests and other broadleaf forests in northeast China were used to demonstrate the application of BAPP. The results showed that the value of BAPP for each sample plot was larger than or equal to the corresponding BARP value for each forest type. For appropriately managed stands with relatively better site conditions, the values of both BARP and BAPP were almost identical. The values of the difference between BAPP and BARP could therefore be used to effectively assess forest site productivity. Meanwhile, BAPP also provides much reliable and valuable information that can aid decision-making in forest management.
语种：
英文
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降香黄檀黑痣病菌的快速分子检测
作者：
刘倩丽;周国英;李河;董文统;刘成锋;...
期刊：
植物病理学报 ,2016年46(1):135-139 ISSN：0412-0914
作者机构：
[刘倩丽; 周国英; 李河; 董文统; 刘成锋; 刘君昂] 中南林业科技大学, 经济林培育与保护教育部重点实验室, 长沙, 410004
关键词：
降香黄檀黑痣病菌;快速;分子检测
摘要：
黄檀黑痣菌(Phyllachora dalbergiicola)能引起降香黄檀黑痣病,该病菌在世界分布十分广泛,且寄主植物种类繁多,除了降香黄檀以外,还可侵染大果紫檀、檀香紫檀等多种紫檀属植物。该菌所致病害会在寄主表面形成黑色凸起的盾片,严重影响寄主光合作用,致使其提前落叶[1]。通过对黄檀黑痣菌进行早期检测,可以及时采取防治措施,控制病害的进一步发展。而黄檀黑痣菌是活体营养真菌,不能离体培养,使用常规的病害诊断技术难以准确快速鉴定该病原菌。因此,建立一种快速、灵敏、准确的黄檀黑痣菌分子检测技术具有重要的实际意义。
语种：
中文
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