通讯机构：
The Key Laboratory for Non-wood Forest Cultivation and Conservation of Ministry of Education, Central South University of Forestry and Technology, China
作者机构：
[李河; 周国英; 章怀云; 刘君昂; 彭宽] Central South University of Forestry and Technology
关键词：
油茶苗圃;炭痕病菌;抗药性;研究
摘要：
The fungicide resistance of anthracnose in Camellia oleifera nurseries was investigated in Liuyang,Changning,and other regions in Hunan Province.The Colletotrichum gloeosporioides strains from the former two regions were highly resistant to carbendazim.After subcultured for 10 generations on fungicide-free medium,the resistant strains grew well on the medium containing carbendazim 450 μg/mL and suggesting that its resistance was stable.The β-tubulin genes from the resistant and susceptible strains were cloned and sequenced.The coding region was 1 344 bp nucleotides and predicted to encode a protein with 447 amino acids.Comparison of the β-tubulin amino acid sequences between resistant and susceptible strains of Colletotrichum gloeosporioides revealed that a mutation leading to an amino acid substitution at the position 198 from glutamic acid in the susceptible strain to alanine in the resistant strain.Finally,the main morphological characteristics of C.gloeosporioides were descripted,but it could not be used to determine the resistance to carbendazim.
作者机构：
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.;[Liu Junang; Zhou Guoying; Guo Liang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
通讯机构：
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Hunan, Peoples R China.
会议名称：
International Symposium on Manufacturing Systems Engineering (ISMSE 2011)
会议时间：
SEP 17-18, 2011
会议地点：
Hong Kong, PEOPLES R CHINA
会议主办单位：
[Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha, Peoples R China.^[Zhou Guoying;Guo Liang;Liu Junang] Cent South Univ Forestry & Technol, Coll Forestry, Changsha, Peoples R China.
会议论文集名称：
Advanced Materials Research
关键词：
L.deliciosus;GAPDH;Bioinformatics analysis
摘要：
Glyceraldehyde 3-phosphate dehydrogenase is an enzyme that catalyzes the sixth step of glycolysis and thus serves to break down glucose for energy and carbon molecules. In the present study, some characters of the amino acid sequence of GAPDH of L.deliciosus were predicted and analyzed with the tools of bioinformatics. These results showed that the protein was composed of 20 kinds of amino acid; the theoretical pI of GAPDH was 7.08 and the theoretical molecular weight of GAPDH was 26165.9 Da; the total number of atoms was 3714. It was a stable protein. There were 7 glycosylation sites and it was a tetrameric NAD-binding enzyme involved in glycolysis and glyconeogenesis. N-terminal domain is a Rossmann NAD (P) binding fold. C-terminal domain is a mixed alpha/antiparallel beta fold.
关键词：
Control method;Pathogenic mechanism;Pathogeny;Pine;Pine wilt disease
摘要：
The pine is an important tree species in China, while the pine wilt disease is a devastating disease of pine treesPine wood nematode(Bursaphelechus xylophilus Steiner & Buhere, 1934 Nickle, 1981) is a
摘要：
Chinese fir (Cunninghamia Lanceolata) is a unique fast growing merchantable timber species in China with good materials, and is a major fast growing timber species in provinces of southern China. With the increase in Chinese fir plantation areas and the improvement in the degree of pure forests, the diseases of Chinese fir are increasing and their damages are getting worse, which have caused great losses and affected the sustainable, rapid and healthy development of forestry in China. This article gives a description of pathogenies, symptoms and occurrence regularities of four important diseases of Chinese fir, including Glomerella cingulata, Pestalotia Sp., Pseudomonas cunninghamiae and Chlorosis, and puts forward the corresponding control measures of these diseases, which provides an important basis for disease control of Chinese fir plantations.
期刊：
African Journal of Biotechnology,2011年10(30):5719-5724 ISSN：1684-5315
通讯作者：
Li Lin
作者机构：
[Liu Jun-Ang; Li Lin; Zhou Guo-Ying] Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China.;[Liu Jun-Ang; Li Lin; Zhou Guo-Ying; Li He] Cent S Univ Forestry & Technol, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
通讯机构：
[Li Lin] C;Cent S Univ Forestry & Technol, Changsha 410004, Hunan, Peoples R China.
关键词：
Chinese fir;Mycorrhizal fungi;Mycorrhizal inocula;Vesicle-abuscular
摘要：
Chinese fir is one of the most important commercial timber species in south China. Mycorrhizal fungi has been applied in many trees and showed important effect on enhancing the productive forces and the soil activities, but there is little report about the mycorrhizal fungi on Chinese fir. So this paper investigated the mycorrhizal fungi resources of Chinese fir in Hunan province and analyzed the relation of community with the environment factors. The results showed that, abuscular mycorrhizal fungi (AMF) existed universally in the roots of Chinese fir. 75 specimen of AMF spores were isolated by wet-sieved method from this collection of the rhizosphere soils. They were identified by morphologic method to 4 genera and 9 species of AMF, which 5 species belong to Glomus, 1 of Gigaspora, 2 of Acaulospora and 1 of Scutellospora. In the isolated AMF, the importance value of Glomus mosseae, Glomus intraradices and Acaulospora laevis were more than 50% and the spore number of them was significantly higher than other species. These 3 species of AMF were considered as the dominate species in rhizosphere soil of Chinese fir in Hunan province. This research also indicated that, mycorrhiza forming was associated with the environment factors. The AMF spores density has the extremely remarkable correlational dependence with vegetation quantity in Chinese Fir forest (r2=0.943, p < 0.05). Mycorrhiza distribution also associated with seasons and forest age; the most active season of mycorrhiza was between spring and summer and the natural AMF infection rate was increased along with the increasing of forest age. The results will not only provide the theory basis for exploitation and using of mycorrhizal resource, but also enhance the survival rate of seedling and prevent the soil degradation and conserve of soil against erosion.Key words: Mycorrhizal fungi, vesicle-abuscular, Chinese fir, mycorrhizal inocula.
作者机构：
[Zhou Guoying; Zhang Huaiyun; Li He] Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.;[Liu Yadi; Liu Wenjin] Cent South Univ Forestry & Technol, Coll Furniture & Art Design, Changsha, Peoples R China.
通讯机构：
[Li He] C;Cent South Univ Forestry & Technol, Biotechnol Core Facil, Coll Forestry, Changsha 410004, Hunan, Peoples R China.
会议名称：
International Conference on Micro Nano Devices, Structure and Computing Systems
会议时间：
NOV 06-07, 2010
会议地点：
Int Sci & Engn Ctr, Singapore, SINGAPORE
会议主办单位：
Int Sci & Engn Ctr
会议论文集名称：
Advanced Materials Research
关键词：
Bamboo fiber;Bleach;Crude fiber;Pretreatment
摘要：
Bamboo fiber is a new natural green environmental protection fiber material with good characteristics of good permeability, unique elasticity, transient absorption, antibacterial, odor resistance and stronger vertical and horizontal intensity. Bamboo pretreatment can improve the biological degumming efficiency of bamboo lignin, reduce microbial degumming time, and reduce production costs. The results show that the removal efficiency of pectin, hemicelluloses and lignin are better after the pretreatment of bamboo mercerization. Through orthogonal experiments, it can be got the optimum combination of bamboo fiber alkali cooking process is A2-B2-C2-D1, namely: concentration of NaOH is 6.0g / L, its time is 60min, temperature 100 °C, and bath ratio 1:20. In these four factors, the greatest impact on the removal of lignin is temperature, followed by is degumming time, then the concentration of alkali, and the bath ratio is the smallest. The best conditions of bamboo fiber bleaching is: hydrogen peroxide 25 ~ 35g / L, temperature 30 ~ 40 °C, treatment 45 ~ 60min. The process of the pretreatment of bamboo is: clip → Roll → high-pressure steaming → roll→ immersion separation → alkali leaching treatment→ scouring → rolling combing →washing→ drying →crude fiber → bleaching.
摘要：
Based on the important position and the special role of experimental teaching in the training process of application-oriented undergraduate talents as well as the complexity of training process and the diversity of training mode, it is required to highlight the cultivation of the practical ability, the innovation sense, the innovation spirit and the innovation ability of students. This research has combined with the construction reform practice of Hunan practical teaching demonstration center which has pointed out the drawbacks of the opening experimental teaching of the traditional forestry microbiology and analyzed the meaning and the characteristics of opening experimental teaching mode. At the same time, it has also proposed the specific implementation methods and the experimental and practical evaluation system as well as explored an opening experimental teaching mode of forestry microbiology which has been attempted in the teaching practice.
摘要：
The basidiomycete Craterellus tubaeformis (Fries) Quélet is an important widespread ectomycorrhizal basidio-mycete found in the Northern Hemisphere. In this study, 12 samples of C. tubaeformis from North America and Europe were analyzed using internal transcribed spacer (ITS) sequences to reveal the correlation between ITS genotypes and geographic locations and to provide molecular evidence for the identification of C. tubaeformis from different habitats in North America and Europe. The analyses identified abundant sequence variations within C. tubaeformis. The length of the ITS region varied from 571 to 640 bp. The proportion of variable sites was 17.6%, and the proportion of parsimony information sites was 16.7%. Phylogenetic analysis showed some correlations between the ITS genotypes and geographic locations of C. tubaeformis; however, some discrepancies between geographical location and affinity were also found. The results indicated that C. tubaeformis from different habitats in North America and Europe underwent genetic drifting and evolved into 2 different species. nrDNA ITS could be a good markers for distinguishing among C. tubaeformis from different habitats, but rational affinity should be determined by associating the available ITS data with other information sources.
摘要：
Lactarius hatsudake is a type of ectomycorrhizal fungus that significantly influences the growth of pine trees. It is widely prevalent in Asian countries and has a high economic value. Artificial cultivation of this fungus has not been achieved as yet; therefore, excessive manual harvesting may cause serious damages to the site of its production. In this study, we analyzed 41 samples of L. hatsudake from south China using internal transcribed spacer (ITS) sequences. By comparing the differences among ITS sequences to identify the haplotype diversity within each population, the relationships among local populations, the relationship between the level of genetic differentiation and geographical separation, and the contributions of local and regional geographical separations to the overall ITS haplotype variation were analyzed. Genetic analysis indicates that ITS sequences obtained from these 41 L. hatsudake samples could be identified as 18 haplotypes, of which 13 haplotypes were contained in only a single sample, whereas the remaining sequence types all were contained in two or more samples. The most common sequence type, haplotype 6, was found in 16 samples and was distributed across nearly every region. The Mantel test demonstrated that there is no significant linear relationship between geographical distance and the FST value of genetic difference. Results of this research illustrates that there exists a certain degree of genetic intermixing among natural populations of L. hatsudake. From the group genetic analysis, it appears that there exists genetic differentiation of lower frequencies in natural populations of L. hatsudake; however, the linear relationship between the degree of genetic differentiation and geographical distance is not distinctly apparent.
