期刊论文

Biological Function bZIP-Type Transcription Factor CfAp1 in Colletotrichum fructicola

中文

林业科学

1001-7488

2020

56

9

30-39

10.11707/j.1001-7488.20200904

湖南省自然科学基金项目(2019JJ40531) 中南林业科技大学大学生科技创新基金(2018-10)。

本校为第一机构

【目的】研究油茶炭疽病的主要流行致病菌果生刺盘孢菌bZIP转录因子CfAp1的生物学功能,以期阐明果生刺盘孢菌致病的分子机制,为油茶炭疽病的防治提供理论依据。【方法】提取果生刺盘孢菌全基因组DNA,根据同源重组原理构建CfAP1基因敲除载体片段,采用PEG介导法把该片段转化至果生刺盘孢菌野生型菌株的原生质体中,验证筛选突变体菌株; PCR扩增果生刺盘孢菌含有启动子的CfAP1基因回补片段,构建回补载体pYF11: : CfAP1;采用PEG介导法把回补载体pYF11: : CfAP1转化至果生刺盘孢菌突变体菌株的原生质体中,获得回补菌株ΔCfap1 /AP1。测定野生型菌株、突变体菌株ΔCfap1-8及基因回补菌株ΔCfap1 /AP1在...

Objectve: Colletotrichum fructicola is one of the major causal pathogens of Camellia oleifera. This experiment aims to study the biological functions of a leucine zipper (bZIP) type transcription factor CfAp1 in order to elucidate the molecular mechanism of C. fructicola, providing theoretical basis for controlling oil-tea tree anthracnose. Method: The whole genomic DNA of C. fructicola was extracted, and the CfAP1 gene knockout vector fragment was constructed based on the principle of homologous recombination. The fragment was transformed into...